Identifikasi Dan Uji Daya Hambat Isolat Bakteri Endofit Bunga Tanaman Cengkeh (Syzygium aromaticum L.) Terhadap Bakteri Patogen

Autor(s): Citra Sepriana, Eti Sumiati, Dwi Soelistya Dyah Jekti, Lalu Zulkifli
DOI: 10.29303/jppipa.v6i1.340

Abstract

This research was conducted to find out the capabilities of endophytic bacteria isolated from flowers of the clove plants in inhibiting the growth of bacteria Streptococcus mutans, Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli and to identify endophytic bacteria that potensial to produce an antibacterial. Stages of this research include the isolation of endophytic bacteria from flowers of the clove plants, antibacterial test, and molecular identification based on 16S rRNA. Isolates of endophytic bacterial of clove plants flower produce 5 isolates, 4 isolate inhibited the bacteria S. aureus. Based on 16S rRNA molecular identification, endophytic bacterial isolates of clove plants flower which have inhibitory closely related to Bacillus amyloliquefasiens, Staphylococcus epidermidis 1034 MPA and Bacillus cereus JL.

Keywords

flowers of the clove plant; endophytic bacterial; molecular identification.

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References

Ali, H.S., M. Kamal and S.B. Mohamed. 2009. Invitro Clove Oil Activity Againts Periodontopathic Bacteria. Journal of Science Technology 10 (1) : 1-7.

Andries, J.R., Gunawan, P.N., Supit, A. 2014. Uji Efek Bakteri Bunga Cengkeh terhadap Bakteri Streptococcus mutans Secara In Vitro. Jurnal e-GIGI (eG). Vol.2 (2): 1-8

Aris, M., Sukenda, Harris, E., Sukadi, MF., Yuhana, M. 2013. Identifikasi molekular bakteri patogen dan desain primer PCR. Budidaya Perairan. Vol. 1, No. 3: 43-50.

Ayoola, G.A., Lawore, F.M., Adelowotan, T., Aibinu, I.E., Adenipekun, E., Coker, H.A.B., Odugbemi, T.O,. 2008. Chemical Analysis and Antimicrobial Activity of The Essential Oil Syzigium aromaticum (Clove). Afr. J. Microbiol. Res. Vol.(2) pp. 162-166.

Backman PA, dan Sikora RA. 2008. Endophytes: an emerging tool for biological control. Biol Control. 46(1):1-3. doi:10.1016/j.bio control.2008.03.009

Balosi F, Lakani I, Panggeso J. 2014. Eksplorasi Bakteri Endofit Sebagai Agens Pengendalian Hayati Terhadap Penyakit Darah Pada Tanaman Pisang Secara In-Vitro. e-J. Agrotekbis. 2 (6) : 579-586.

Brooks, Geo, Janet S. Butel, L. Nicholas Ornston. 2005. Mikrobilologi Kedokteran. Jakarta: EGC.

Handini ZVT dan Nawangsih AA., 2014. Keefektifan bakteri endofit dan bakteri perakaran pemacu pertumbuhan tanaman dalam menekan penyakit layu bakteri pada tomat. Jurnal fitopatologi Indonesia. ISSN: 2339-2479. Vol. 10 No. 2

Herliana, E. 2013. Penyakit Asam Urat Kandas Berkat Herbal. FMedia (Imprint Agro Media Pustaka). Jakarta.

Herzner, A. M., J. Dischinger, C. Szekat, M. Josten, S. Schmitz, A. Yake ́le ́ba, R. Reinartz, A. Jansen, H. G. Sahl, J. Piel and G. Bierbaum . 2011. Expression of the Lantibiotic Mersacidin in Bacillus amyloliquefaciens FZB42. Plos one Journal, 6(1): 1-8.

Kumala, S.dan Indriani, D., 2008. Efek Antibakteri Ekstrak Etanol Daun Cengkeh (Eugenia aromaticum L). Jurnal Farmasi Indonesia. Vol.4. (2): 82-87.

Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC, Hiom SJ, Wade WG. 1998. Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ. Microbiol 64:795-9.

Pramod, K., Ansari, S.H., Ali., J. 2010. Eugenol: A Natural Compound with Versatile Pharmacological Actions. J. Natural Product Communications 5(12) : 199-206.

Prayoga, E. 2013.Perbandingan Efek Ekstrak Daun Sirih Hijau (Piper betle L) Dengan Metode Difusi Disk Dan Sumuran Terhadap Pertumbuhan Bakteri Staphylococcus aureus.Fakultas Kedokteran dan Ilmu Kesehatan Universitas Islam Negeri Syarif Hidayatullah. Jakarta.

Rochyani, L., Aprilia dan M.W. Astuti. .2007. Daya Anti Bakteri Bahan Tumpatan Sementara Zinc Oxide Eugenol. DENTAL Jurnal Kedokteran Gigi FKG-UHT 1(2) : 96-99.

Sepriana, C., jekti, DSD., Zulkifli,L. 2017. Bakteri Endofit Kulit Batang Dan Bunga Tanaman Cengkeh (Syzygium aromaticum L.) Dan Kemampuannya Sebagai Antibakteri. Jurnal Penelitian Pendidikan IPA. Vol.3 (2).

Simarmata R, Lekatompessy S, Sukiman H. 2007. Isolasi Mikroba Endofitik Dari Tanaman Obat Sambung Nyawa (Gymura procumbens) Dan Analisis Potensinya Sebagai Antimikroba. Berk. Penel. Hayati: 13 (85-90).

Strobel, G.A., 2003. Endophytes as Sources of Bioactive Products, Microb.Infect., 5, 535–544.

Sumampouw, M., Bara R., Awaloei H., Posangi J., 2014. Uji Efek Antibakteri Jamur Endofit Akar Bakau Rhizophora stylosa Terhadap Bakteri Staphylococcus aureus dan Escherichia coli. Bagian Farmakologi Dan Terapi Fakultas Kedokteran Universitas Sam Ratulangi.

Sumiati, E., 2014. Uji Aktivitas Antibakteri Ekstrak Kloroform dan Ekstrak Etanol Bii Bidara Laut (Strichnos ligustrina BI) terhadap Staphylococcuc aureus ATCC 25923 dan Salmonella thypi. Jurnal Ilmiah Biogenesis. Vol. 2 (1): 1-10.

Suryadi Y, Samudra IM, Priyatno TP, Susilowati, DN, Lestari P, Sutoro. 2015. Aktivitas Anticendawan Bacillus cereus 11UJ terhadap. Jurnal Fitopatologi. ISSN: 0215-7950 Volume 11, Nomor 2

Susanto, D. Sudrajat dan R. Ruga. 2012. Studi kandungan bahan aktif tumbuhan meranti merah (Shorea leprosula Miq) sebagai sumber senyawa antibakteri. Mulawarmnan Scientifie 11(2): 181-190.

Tan, R.X and Zou, W.X. (2001). Endophytes: a Such Source of Functional Metabolites. Natural Product Rep. 18:448-459.

Ward, D.M. 1998. A natural species concepts for procaryotes. Current Opinion in Microbiology 1: 271-277.

Yuan J, Raza W, Shen Q, Huang Q. 2012. Antifungal activity of Bacillus amyloliquefaciens NJN-6 volatile compounds against Fusarium oxysporum f. sp. cubense. Appl Environ Microbiol.78(16):5942-5944 DOI: http://dx.doi.org/10.1128/AEM.01357-12

Zinniel DK, Lambrecht P, Beth Harris N, Feng Z, Kuczmarski D, Higley P, Ishimaru CA, Arunakumari A, Barletta RG, Vidaver AK. 2002. Isolation and characterization of endophytic colonizing bacteria from agronomic crops and prairie plants. Appl Environ Microbiol. 68(5):2198-2208.

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