Vol. 8 No. 1 (2022): January
Open Access
Peer Reviewed

Characterization of PVA-Enzyme Coated Indicator Electrodes GA coated again with PVC-KTpClPB-o-NPOE SEM-EDS, FTIR and XRD analysis

Authors

Abd Hakim S

DOI:

10.29303/jppipa.v8i1.1265

Published:

2022-01-08

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Abstract

This study aims to characterize the tungsten-urea analyte indicator electrode. The method used is biosensor potentiometry with urease enzyme immobilization technique. This indicator electrode was coated with PVA-enzyme coated with glutaraldehyde (GA) 2.9% coated with PVC-KTpClPB- o-NPOE with o-NPOE variation of 61% and 66%. Characterization of coated indicator electrodes using SEM-EDS, FTIR and XRD analysis. A1-4 61% indicator electrode sample coated PVA-enzyme 1x coated with glutaraldehyde (GA) 2.9% 1x coated PVC-KTpClPB- o-NPOE 1x, with o-NPOE 61%. A3-4 61% indicator electrode sample coated PVA-enzyme 3x coated with glutaraldehyde (GA) 2.9% 1x coated PVC-KTpClPB- o-NPOE 1x, with o-NPOE 61%. Likewise, the reasoning of samples A1-4 66% and A3-4 66%. There are four indicator electrodes made with the notation A1-4 61%, A1-4 66%, A3-4 61% and A3-4 66%. The best results were obtained at the indicator electrode sample A1-4 61%, contributing to the urea sensor of the potentiometer cell

Keywords:

Tungsten indicator electrodes GA and o-NPOE SEM-EDS FTIR and XRD Biosensor Potentiometry Immobilization

References

Alarfaj, N. A., & El-Tohamy, M. F. (2020). New Functionalized Polymeric Sensor Based NiO/MgO Nanocomposite for Potentiometric Determination of Doxorubicin Hydrochloride in Commercial Injections and Human Plasma. In Polymers 12(12). https://doi.org/10.3390/polym12123066.

Alharthi, S. S., Fallatah, A. M., & Al-Saidi, H. M. (2021). Design and Characterization of Electrochemical Sensor for the Determination of Mercury(II) Ion in Real Samples Based upon a New Schiff Base Derivative as an Ionophore. In Sensors 21(9). https://doi.org/10.3390/s21093020.

Al-Mohaimeed, A. M., Mostafa, G. A. E., & El-Tohamy, M. F. (2021). New Construction of Functionalized CuO/Al2O3 Nanocomposite-Based Polymeric Sensor for Potentiometric Estimation of Naltrexone Hydrochloride in Commercial Formulations. In Polymers 13(24). https://doi.org/10.3390/polym13244459.

Amr, A., Kamel, A. H., Almehizia, A. A., Sayed, A., & Abd-Rabboh, H. (2021). Solid-Contact Potentiometric Sensors Based on Main-Tailored Bio-Mimics for Trace Detection of Harmine Hallucinogen in Urine Specimens. Molecules (Basel, Switzerland), 26(2), 324. https://doi.org/10.3390/molecules26020324.

Badakhshan, S., Ahmadzadeh, S., Mohseni-Bandpei, A., Aghasi, M., & Basiri, A. (2019). Potentiometric sensor for iron (III) quantitative determination: experimental and computational approaches. BMC Chemistry, 13(1), 131. https://doi.org/10.1186/s13065-019-0648-x.

Elbehery, N. H. A., Amr, A. E.-G. E., Kamel, A. H., Elsayed, E. A., & Hassan, S. S. M. (2019). Novel Potentiometric 2,6-Dichlorophenolindo-phenolate (DCPIP) Membrane-Based Sensors: Assessment of Their Input in the Determination of Total Phenolics and Ascorbic Acid in Beverages. In Sensors 19(9). https://doi.org/10.3390/s19092058.

El-Naby, E. H. (2019). Potentiometric Signal Transduction for Selective Determination of 1-(3-Chlorophenyl)piperazine “Legal Ecstasy†Through Biomimetic Interaction Mechanism. In Chemosensors 7(3). https://doi.org/10.3390/chemosensors7030046.

Golcs, Ã., Horváth, V., Huszthy, P., & Tóth, T. (2018). Fast Potentiometric Analysis of Lead in Aqueous Medium under Competitive Conditions Using an Acridono-Crown Ether Neutral Ionophore. In Sensors 18(5). https://doi.org/10.3390/s18051407.

Hakim S, A., Sebayang, K., Situmorang, M., Tarigan, K., Sembiring, K., Mihardi, S., & Waruwu, E. (2021). Characterization of indicator electrodes using the potentiometric biosensor method as urea sensor with XRD, SEM-EDS, UV-Vis and FTIR. Journal of Physics: Conference Series, 1816, 12088. https://doi.org/10.1088/1742-6596/1816/1/012088.

Hakim, S.A., Sembiring, T., Tarigan, K., Sebayang, K., Situmorang, M., & Noer, N.M. (2019). Characterization of Membrane PVA-Enzyme Coated PVC-KTpClPB as Urea Sensor with Potentiometric Method. Rasayan Journal of Chemistry, 12, 780–786. https://doi.org/10.31788/RJC.2019.1225143

Hakim, S.A., Tarigan, K., Situmorang, M., & Sembiring, T. (2018). Synthesis of Urea Sensors using Potentiometric Methods with Modification of Electrode Membranes Indicators of ISE from PVA-Enzymes Coating PVC-KTpClPB. Journal of Physics: Conference Series, 1120, 12024. https://doi.org/10.1088/1742-6596/1120/1/012024.

Hassan, S. S. M., H. Kamel, A., Amr, A. E.-G. E., Abd-Rabboh, H. S. M., Al-Omar, M. A., & Elsayed, E. A. (2020). A New Validated Potentiometric Method for Sulfite Assay in Beverages Using Cobalt(II) Phthalocyanine as a Sensory Recognition Element. In Molecules 25(13). https://doi.org/10.3390/molecules25133076.

Hassan, S., Abdelbasir, S. M., Fathy, M. A., Amr, A., Al-Omar, M. A., & Kamel, A. H. (2019). Gold Plate Electrodes Functionalized by Multiwall Carbon Nanotube Film for Potentiometric Thallium(I) Detection. Nanomaterials (Basel, Switzerland), 9(8), 1160. https://doi.org/10.3390/nano9081160.

Hassan, S., H Kamel, A., Amr, A., Hashem, H. M., & Bary, E. (2020). Imprinted Polymeric Beads-Based Screen-Printed Potentiometric Platforms Modified with Multi-Walled Carbon Nanotubes (MWCNTs) for Selective Recognition of Fluoxetine. Nanomaterials (Basel, Switzerland), 10(3), 572. https://doi.org/10.3390/nano10030572.

Huang, S., Luo, F., & Lai, X. (2014), Novel Potentiometric Sensors of Ion Imprinted Polymers for Specific Binding of Yttrium (III), Asian Journal of Chemistry; 26(20). 6787-6790. https://doi.org/10.14233/ajchem.2014.16795

Kamel, A. H., Amr, A. E.-G. E., Ashmawy, N. H., Galal, H. R., Almehizia, A. A., Youssef, T. A., Al-Omar, M. A., & Sayed, A. Y. A. (2020). Validation of a Novel Potentiometric Method Based on a Polymeric PVC Membrane Sensor Integrated with Tailored Receptors for the Antileukemia Drug Cytarabine. In Polymers 12(6). https://doi.org/10.3390/polym12061343.

Kaur, H., Chhibber, M., & Mittal, S. K. (2017). Acyclic Arylamine-Based Ionophores as Potentiometric Sensors for Zn2+ and Ni2+ Ions. In C. 3(4). https://doi.org/10.3390/c3040034.

Mir, M., Lugo, R., Tahirbegi, I. B., & Samitier, J. (2014). Miniaturizable Ion-Selective Arrays Based on Highly Stable Polymer Membranes for Biomedical Applications. In Sensors 14(7). https://doi.org/10.3390/s140711844.

Pomećko, R., Luboch, E., & Jeszke, M. (2021). Dipodal Tetraamide Derivatives of 1,10-Diaza-18-Crown-6 and Alkylmalonic Acids-Synthesis and Use as Ionophores in Ion Selective Membrane Electrodes. Sensors (Basel, Switzerland), 21(15), 4984. https://doi.org/10.3390/s21154984.

Rahman, M. A., Kumar, P., Park, D.-S., & Shim, Y.-B. (2008). Electrochemical Sensors Based on Organic Conjugated Polymers. Sensors, 8(1), 118–141. https://doi.org/10.3390/s8010118.

Rizk, N. M., Abbas, S. S., Hamza, S. M., & Abd El-Karem, Y. M. (2009). Thiopental and Phenytoin as Novel Ionophores for Potentiometric Determination of Lead (II) Ions. Sensors (Basel, Switzerland), 9(3), 1860–1875. https://doi.org/10.3390/s90301860.

Shawky, A. M., & El-Tohamy, M. F. (2021). Highly Functionalized Modified Metal Oxides Polymeric Sensors for Potentiometric Determination of Letrozole in Commercial Oral Tablets and Biosamples. Polymers, 13(9), 1384. https://doi.org/10.3390/polym13091384.

Ulianas, A., Heng, L. Y., & Ahmad, M. (2011). A biosensor for urea from succinimide-modified acrylic microspheres based on reflectance transduction. Sensors (Basel, Switzerland), 11(9), 8323–8338. https://doi.org/10.3390/s110908323

Author Biography

Abd Hakim S, Universitas Negeri Medan

Author Origin : Indonesia

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How to Cite

S, A. H. (2022). Characterization of PVA-Enzyme Coated Indicator Electrodes GA coated again with PVC-KTpClPB-o-NPOE SEM-EDS, FTIR and XRD analysis. Jurnal Penelitian Pendidikan IPA, 8(1), 103–108. https://doi.org/10.29303/jppipa.v8i1.1265