TY - JOUR AU - Anarkhis, Nuranti AU - Prajitno, Arief AU - Maftuch, Maftuch AU - Karimah, Karimah PY - 2023/02/28 Y2 - 2024/03/29 TI - Activity Test of Antibacterial Compounds of Aloe Vera Extract Against Edwardsiella Tarda Bacterial Infection in Vitro (technique) JF - Jurnal Penelitian Pendidikan IPA JA - jppipa, pendidikan ipa, fisika, biologi, kimia VL - 9 IS - 2 SE - Research Articles DO - 10.29303/jppipa.v9i2.2610 UR - https://jppipa.unram.ac.id/index.php/jppipa/article/view/2610 SP - 563-567 AB - <p><em>Edwardsiella tarda </em>is a type of gram-negative intracellular pathogen that can cause mass demise in fish farming. The development of edwarsiellosis due to <em>E.tarda </em>infection causes significant economic losses for cultivators. Aloe vera is a perennial succulent plant like a cactus, drought withstand, and belongs to the Liliaceae family, of which there are more than 360 known species (Wijaya and Masfufatun, 2022). <em>Aloe vera </em>is one of the plants known to contain active ingredients that can inhibit the growth of bacteria. The results of phytochemical analysis of a crude extract of <em>Aloe vera </em>showed the presence of active compounds, specifically flavonoids, tannins, triterpenoids, and saponins. MIC analysis showed that the lowest absorbance value of <em>Aloe vera </em>was obtained at a concentration of 50 mg/L with a value of 0.316, indicating a decrease at each concentration. The concentration of 50 mg/L is close to the positive control which indicates that at a concentration of 50 mg/L, it can be the reference for the lowest concentration in determining the dose of <em>Aloe vera </em>extract which is known that the presence of antibacterial compounds in <em>Aloe vera </em>extract can inhibit the growth of <em>E.tarda</em> bacteria. The inhibition zone test was carried out with graded concentrations of 50 mg/L, 100 mg/L, 150 mg/L, 200 mg/L and 250 mg/L with an observation time of 24 hours post-incubation and 48 hours post-incubation. Inhibition zone analysis with the highest diameter was at 200 mg/L. SEM analysis by distributing a crude extract of <em>Aloe vera </em>affected <em>E.tarda </em>bacteria specifically, lysis occurring in bacterial cells</p> ER -