Determination of SPF (Sun Protection Factor) Value in Sunscreen Formulation of Vetiveria Zizanioides L. Essential Oil
DOI:
10.29303/jppipa.v11i4.10893Published:
2025-04-25Issue:
Vol. 11 No. 4 (2025): AprilKeywords:
Formulation, In vitro, Spectrophotometry, Sun protection factor (SPF), Sunscreen, Vetiver essential oil (Vetiveria zizanioides)Research Articles
Downloads
How to Cite
Downloads
Metrics
Abstract
This research aimed to determine the in vitro Sun Protection Factor (SPF) value of sunscreen formulations containing vetiver essential oil (Vetiveria zizanioides) at various concentrations. Vetiver essential oil is known to possess various biological activities, including potential as a UV protective agent. Oil-in-water emulsion-based sunscreen formulations were prepared with varying concentrations of vetiver essential oil (1%, 3%, and 5% v/v). The SPF value was determined spectrophotometrically using the in vitro method. The physicochemical characteristics of the formulations (viscosity and pH) were also evaluated. The results showed that the addition of vetiver essential oil tended to significantly increase the SPF value of the sunscreen formulations with increasing concentration. The formulation with a 5% concentration of vetiver essential oil exhibited the highest SPF value. The physicochemical characteristics of the formulations were still within the acceptable range for topical preparations. This research indicates the potential of vetiver essential oil as a natural UV protective agent in sunscreen formulations.
References
Amorati, R., Foti, M. C., & Valgimigli, L. (2013). Antioxidant Activity of Essential Oils. Journal of Agricultural and Food Chemistry, 61(46), 10835–10847. https://doi.org/10.1021/jf403496k
Berry, E. G., Bezecny, J., Acton, M., Sulmonetti, T. P., Anderson, D. M., Beckham, H. W., Durr, R. A., Chiba, T., Beem, J., Brash, D. E., Kulkarni, R., Cassidy, P. B., & Leachman, S. A. (2022). Slip versus Slop: A Head-to-Head Comparison of UV-Protective Clothing to Sunscreen. Cancers, 14(3), 542. https://doi.org/10.3390/cancers14030542
Camara, A. K. S., Lesnefsky, E. J., & Stowe, D. F. (2010). Potential Therapeutic Benefits of Strategies Directed to Mitochondria. Antioxidants & Redox Signaling, 13(3), 279–347. https://doi.org/10.1089/ars.2009.2788
Chauhan, A., Yadav, A., Chauhan, A., Chauhan, A., Mishra, S., Rai, J., & Jena, J. (2023). a Review: Sun Protecting Factor. Ymer, 22, 13. Retrieved from https://shorturl.at/KhsxJ
Cole, C. (2016). Sunscreen Formulation: Optimizing Efficacy of UVB and UVA Protection. In Principles and Practice of Photoprotection (Vol. 38, Issue 1, pp. 275–287). Springer International Publishing. https://doi.org/10.1007/978-3-319-29382-0_15
D’Orazio, J., Jarrett, S., Amaro-Ortiz, A., & Scott, T. (2013). UV radiation and the skin. International Journal of Molecular Sciences, 14(6), 12222–12248. https://doi.org/10.3390/ijms140612222
Diffey, B., & Osterwalder, U. (2017). Labelled sunscreen SPFs may overestimate protection in natural sunlight. Photochemical & Photobiological Sciences, 16(10), 1519–1523. https://doi.org/10.1039/c7pp00260b
Diniz do Nascimento, L., Moraes, A. A. B. de, Costa, K. S. da, Pereira Galúcio, J. M., Taube, P. S., Costa, C. M. L., Neves Cruz, J., de Aguiar Andrade, E. H., & Faria, L. J. G. de. (2020). Bioactive Natural Compounds and Antioxidant Activity of Essential Oils from Spice Plants: New Findings and Potential Applications. Biomolecules, 10(7), 988. https://doi.org/10.3390/biom10070988
González, S., Aguilera, J., Berman, B., Calzavara-Pinton, P., Gilaberte, Y., Goh, C.-L., Lim, H. W., Schalka, S., Stengel, F., Wolf, P., & Xiang, F. (2022). Expert Recommendations on the Evaluation of Sunscreen Efficacy and the Beneficial Role of Non-filtering Ingredients. Frontiers in Medicine, 9. https://doi.org/10.3389/fmed.2022.790207
Grover, M., Behl, T., Virmani, T., Bhatia, S., Al-Harrasi, A., & Aleya, L. (2021). Chrysopogon zizanioides—a review on its pharmacognosy, chemical composition and pharmacological activities. Environmental Science and Pollution Research, 28(33), 44667–44692. https://doi.org/10.1007/s11356-021-15145-1
Li, L., Chong, L., Huang, T., Ma, Y., Li, Y., & Ding, H. (2023). Natural products and extracts from plants as natural UV filters for sunscreens: A review. Animal Models and Experimental Medicine, 6(3), 183–195. https://doi.org/10.1002/ame2.12295
Liu, S., Huang, W., Yang, J., Xiong, Y., Huang, Z., Wang, J., Cai, T., Dang, Z., & Yang, C. (2023). Formation of environmentally persistent free radicals on microplastics under UV irradiations. Journal of Hazardous Materials, 453, 131277. https://doi.org/10.1016/j.jhazmat.2023.131277
Mejía‐Giraldo, J. C., Gallardo, C., & Puertas‐Mejía, M. A. (2022). Selected Extracts from High Mountain Plants as Potential Sunscreens with Antioxidant Capacity. Photochemistry and Photobiology, 98(1), 211–219. https://doi.org/10.1111/php.13490
Meullemiestre, A., Kamal, I., Maache-Rezzoug, Z., Chemat, F., & Rezzoug, S. A. (2014). Antioxidant Activity and Total Phenolic Content of Oils Extracted from Pinus pinaster Sawdust Waste Screening of Different Innovative Isolation Techniques. Waste and Biomass Valorization, 5(2), 283–292. https://doi.org/10.1007/s12649-013-9237-8
Ng, S. Y., Eh Suk, V. R., & Gew, L. T. (2022). Plant polyphenols as green sunscreen ingredients: A systematic review. Journal of Cosmetic Dermatology, 21(11), 5409–5444. https://doi.org/10.1111/jocd.15170
Pandey, A., & Tiwari, S. C. (2024). A Review on Chemical Composition, Oil Quality, and Bioactivity of Vetiver Essential Oil. Indian Journal of Pharmaceutical Sciences, 86(4). https://doi.org/10.36468/pharmaceutical-sciences.1381
Sayre, R. M., Agin, P. P., LeVee, G. J., & Marlowe, E. (1979). A Comparison of in Vivo and in Vitro Testing of Sunscreening Formulas. Photochemistry and Photobiology, 29(3), 559–566. https://doi.org/10.1111/j.1751-1097.1979.tb07090.x
Shrivastava, R., & Agnihotri, R. K. (2024). Revealing the shielding effect of vetiver (Chrysopogon zizanioides) essential oil and their possible use in cosmeceuticals. Medicinal Plants - International Journal of Phytomedicines and Related Industries, 16(1), 38–45. https://doi.org/10.5958/0975-6892.2024.00004.2
Thomas, O., & Brogat, M. (2022). Organic constituents. In UV-Visible Spectrophotometry of Waters and Soils (pp. 95–160). Elsevier. https://doi.org/10.1016/B978-0-323-90994-5.00006-X
Xanthis, V., Fitsiou, E., Voulgaridou, G.-P., Bogadakis, A., Chlichlia, K., Galanis, A., & Pappa, A. (2021). Antioxidant and Cytoprotective Potential of the Essential Oil Pistacia lentiscus var. chia and Its Major Components Myrcene and α-Pinene. Antioxidants, 10(1), 127. https://doi.org/10.3390/antiox10010127
Yeşildağ, A., Erdoğan, M., Medetalibeyoğlu, H., & Horoz, S. (2022). Synthesis of benzidine-based conjugated organic materials bearing donor-acceptor groups: DFT studies and photovoltaic applications. Journal of Molecular Structure, 1251, 131939. https://doi.org/10.1016/j.molstruc.2021.131939
Author Biographies
Waode Suiyarti, Universitas Muhammadiyah Palopo
Ervianingsih, Universitas Muhammadiyah Palopo
Chitra Astari, Universitas Muhammadiyah Palopo
Riska, Universitas Muhammadiyah Palopo
Sunarto S, Universitas Muhammadiyah Palopo
License
Copyright (c) 2025 Waode Suiyarti, Ervianingsih, Chitra Astari, Riska, Sunarto S

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).