Characterization of Orchid Root Fungal Isolates as Leaflet Teaching Material to Improve Learning Outcomes of Grade X Vocational High School Students on the Subtopic of Kingdom Fungi
DOI:
10.29303/jppipa.v11i5.10934Published:
2025-05-25Issue:
Vol. 11 No. 5 (2025): MayKeywords:
Kingdom fungi, Learning outcomes, Orchid rootsResearch Articles
Downloads
How to Cite
Downloads
Metrics
Abstract
This study aimed to identify mycorrhizal fungi, primarily from the genera Rhizoctonia and Tulasnella, on orchid roots and develop leaflet-based teaching materials using the Research and Development (R&D) model with the 4D approach (Define, Design, Develop, Disseminate). Fungal identification was conducted through microscopic examination and molecular techniques (ITS rDNA sequencing) to ensure accurate classification. The 4D model involved defining student needs, designing leaflet content focused on fungal symbiosis, developing draft materials, and disseminating the final leaflet for classroom use. The effectiveness of the leaflet was evaluated with 40 students through pre- and post-tests measuring knowledge of fungal roles in orchid growth. Results showed the average pre-test score increased from 48.5 to 85.2 in the post-test, with an N-Gain score of 0.722 (72.29%), indicating moderate effectiveness. Improvements were most notable in students’ understanding of fungal identification and symbiotic functions. These findings demonstrate that leaflet-based teaching materials developed from precise fungal identification can significantly enhance student comprehension of Kingdom Fungi concepts.
References
Aisha, H., Kresnadi, H., & Bistari, B. (2023). Pengembangan Leaflet Dikaji dalam Dimensi Bernalar Kritis di Kelas IV SD Negeri 39 Pontianak Kota. As-Sabiqun, 5(4), 931–947. https://doi.org/10.36088/assabiqun.v5i4.3518
Akbar, S. (2013). Instrumen Perangkat Pembelajaran. Bandung: PT Rosdakarya Remaja.
Apriana, D. (2023). Pengembangan Media Pembelajaran Booklet Materi Sistem Gerak pada Manusia Kelas XI MA Ma’arif 9 Kotagajah (Undergraduate Thesis). IAIN Metro. Retrieved from https://repository.metrouniv.ac.id/id/eprint/8238/
Aulya, S. A. N. (2020). Eksplorasi Jamur Endofit pada Akar dan Daun Anggrek Dendrobium (Dendrobium sp.) dan Potensi Antagonismenya Terhadap Penyakit Bercak Daun Cecospora (Cercospora sp.). Universitas Brawijaya Malang. Retrieved from https://repository.ub.ac.id/id/eprint/181140
Aziz, M. R. R. S. (2017). Uji Aktivitas Antibakteri Fungi Endofit dari Buah Tanaman Nangka Muda (Artocarpus heterophylus Lamk) Terhadap Staphylococcus aureus, Shigella dysentriae, dan Escherichia coli (Thesis). UIN Syarif Hidayatullah Jakarta: Fakultas Kedokteran dan Ilmu Kesehatan. Retrieved from https://repository.uinjkt.ac.id/dspace/bitstream/123456789/37353/1/m.%20rizal%20rosyidi%20sulistyo%20aziz-fkik.pdf
Basir, R. S., & Rohmawati, I. (2023). Penerapan Model Pembelajaran Discovery Learning Berbantuan Leaflet untuk Meningkatkan Hasil Belajar Kognitif Siswa Kelas VIII. Educatoria: Jurnal Ilmiah Ilmu Pendidikan, 3(4), 262–269. https://doi.org/10.36312/educatoria.v3i4.221
Erliza, M. (2023). Karakterisasi dan Uji Potensi Jamur Endofit pada Daun Bawang Merah (Allium ascalonicum L.) sebagai Pengendali Patogen Fusarium sp. dan Alternaria sp. (Doctoral Dissertation). UIN Ar-Raniry Fakultas Sains dan Teknologi. Retrieved from https://journal.arraniry.ac.id/index. hp/kenanga/article/download/3120/1520
Grabka, R., d’Entremont, T. W., Adams, S. J., Walker, A. K., Tanney, J. B., Abbasi, P. A., & Ali, S. (2022). Fungal Endophytes and Their Role in Agricultural Plant Protection Against Pests and Pathogens. Plants, 11(3), 384. https://doi.org/10.3390/plants11030384
Hamzah, P., Subandiyah, S., Wibowo, A., & Farhanah, A. (2021). Variabilitas Morfologi Rhizoctonia solani Penyebab Penyakit Hawar Pelepah Padi di Sulawesi Selatan: Morphological Variability of Rhizoctonia solani Causes of Rice Sheath Blight Disease in South Sulawesi. Jurnal Agrisistem, 17(1), 40-45. https://doi.org/10.52625/j-agr.v17i1.192
Jagat, L. M. S. S., Darmayasa, I. B. G., & Wijana, I. M. S. (2021). Potensi Rhizopus sp. dalam Mengendalikan Pertumbuhan Aspergillus flavus FNCC6109 pada Pakan Konsentrat Ayam Broiler. Jurnal Biologi Udayana, 25(2), 147-156. Retrieved from https://scholar.archive.org/work/4vfsofxutjgovkbzzugg2bjv64/access/wayback/https://ojs.unud.ac.id/index.php/bio/article/download/71565/42343
Jalil, M. (2021). Perencanaan dan Strategi Pembelajaran Biologi. Farha Pustaka. Retrieved from http://opac.iainkudus.ac.id/slims/index.php?p=show_detail&id=552686&keywords=
Kamil, A. L. (2023). Pengembangan Buku Saku Fungi untuk Peserta Didik Kelas X MA Darul A’Mal (Undergraduate Thesis). IAIN Metro. Retrieved from https://repository.metrouniv.ac.id/id/eprint/9271/
Kurniawan, A. B., & Hidayah, R. (2020). Kepraktisan Permainan Zuper Abase Berbasis Android Sebagai Media Pembelajaran Asam Basa. UNESA Journal of Chemical Education, 9(3), 317–323. https://doi.org/10.26740/ujced.v9n3.p317-323
Kuway, N. P., Muhajir, M., & Wahid, A. (2023). Meningkatkan Minat Peserta Didik dalam Mata Pelajaran IPAS Melalui Pembelajaran Diferensiasi Menggunakan Bahan Ajar Digital. Jurnal Basicedu, 7(6), 3869-3877. https://doi.org/10.31004/basicedu.v7i6.6490
Liao, C., Doilom, M., Jeewon, R., Hyde, K. D., Manawasinghe, I. S., Chethana, K. W., ... & Dong, W. (2025). Challenges and Update on Fungal Endophytes: Classification, Definition, Diversity, Ecology, Evolution and Functions. Fungal Diversity, 131(1), 301-367. https://doi.org/10.1007/s13225-025-00550-5
Lubis, A. A. P. (2024). Pengembangan Bahan Ajar Berbasis Leaflet pada Pembelajaran Teks Puisi dalam Menghasilkan Buku Antologi Puisi Kelas XI MA Islamic Centre (Undergraduate Thesis). Fakultas Keguruan & Ilmu Pendidikan, Universitas Islam Sumatera Utara. Retrieved from https://repository.uisu.ac.id/handle/123456789/3673
Magdalena, I., Prabandani, R. O., Rini, E. S., Fitriani, M. A., & Putri, A. A. (2020). Analisis Pengembangan Bahan Ajar. Nusantara, 2(2), 180-187. https://doi.org/10.36088/nusantara.v2i2.805
Maulana, M. A. (2017). Pengembangan Media Pembelajaran Berbasis Leaflet pada Materi Sistem Sirkulasi Kelas XI MAN I Makassar (Undergraduate Thesis). Universitas Islam Negeri Alauddin Makassar. Retrieved from http://repositori.uin-alauddin.ac.id/8440/1/MARWAH%20AHMAD%20MAULANA.pdf
Michael, M., Sukarno, N., Mursidawati, S., Sandra, E., & Rahayu, N. D. (2023). Isolasi dan Identifikasi Cendawan Endofit Akar Anggrek Epifit dan Hemiepifit. Jurnal Sumberdaya Hayati, 9(4), 152–163. https://doi.org/10.29244/jsdh.9.4.152-163
Mizana, D. K., Suharti, N., & Amir, A. (2016). Identifikasi Pertumbuhan Jamur Aspergillus sp. Pada Roti Tawar yang Dijual di Kota Padang Berdasarkan Suhu dan Lama Penyimpanan. Jurnal Kesehatan Andalas, 5(2). https://doi.org/10.25077/jka.v5i2.521
Murdiyah, S. (2017). Endophytic Fungi of Various Medicinal Plants Collected from Evergreen Forest Baluran National Park and Its Potential as Laboratory Manual for Mycology Course. JPBI (Jurnal Pendidikan Biologi Indonesia), 3(1), 64–71. https://doi.org/10.22219/jpbi.v3i1.3977
Munarti, M., Retnowati, R., & Putri, S. A. (2023). Keanekaragaman Jamur Makroskopis sebagai Bahan Ajar Pengayaan Biologi. Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning, 20(1), 49-53. Retrieved from https://jurnal.uns.ac.id/prosbi/article/view/82556
Nurliana, N., & Anggraini, N. (2018). Eksplorasi dan Identifikasi Trichoderma sp. Lokal dari Rizosfer Bambu dengan Metode Perangkap Media Nasi. Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan, 2(2), 41-44. http://dx.doi.org/10.31604/jap.v2i2.516
Prajapati, C., Rai, S. N., Singh, A. K., Chopade, B. A., Singh, Y., Singh, S. K., ... & Ashraf, G. M. (2025). An Update of Fungal Endophyte Diversity and Strategies for Augmenting Therapeutic Potential of their Potent Metabolites: Recent Advancement. Applied Biochemistry and Biotechnology, 1-68. Retrieved from https://link.springer.com/article/10.1007/s12010-024-05098-9
Purnama, I., Rusmiyanto, E., Wardoyo, P., & Linda, R. (2016). Jenis-Jenis Anggrek Epifit di Hutan Bukit Luncit Kecamatan Anjongan Kabupaten Mempawah. Protobiont, 5(2), 1–10. https://doi.org/10.26418/protobiont.v5i2.1693
Ramadhani, Q. R. (2022). Pengembangan Lembar Kerja Peserta Didik (LKPD) Materi Penjumlahan untuk Meningkatkan Kemampuan Menyelesaikan Soal Cerita Peserta Didik Kelas I SDN Pendem 1 (Undergraduate Thesis). Universitas Islam Negeri Maulana Malik Ibrahim. Retrieved from http://etheses.uin-malang.ac.id/68715/
Rindayana, R. (2024). Pengembangan Bahan Ajar Matematika Berbentuk Leaflet Berdasarkan Teori Bruner untuk Meningkatkan Minat Belajar Siswa Kelas X MAN 1 Luwu Timur (Doctoral Dissertation). Institut Agama Islam Negeri Palopo. Retrieved from https://repository.iainpalopo.ac.id/id/eprint/9833/
Rodriguez, R. J., White Jr, J. F., Arnold, A. E., & Redman, R. S. (2009). Fungal Endophytes: Diversity and Functional Roles. Annual Review of Microbiology, 62, 567-588. https://doi.org/10.1146/annurev.micro.62.081307.162918
Saldan, N. C., Almeida, R. T., Avincola, A., Porto, C., Galuch, M. B., Magon, T. F., ... & Oliveira, C. C. (2018). Development of an Analytical Method for Identification of Aspergillus flavus Based on Chemical Markers Using HPLC-MS. Food Chemistry, 241, 113-121. https://doi.org/10.1016/j.foodchem.2017.08.065
Salsabila, N. R. (2024). Kapang Endofit Akar Anggrek Tanah di Taman Nasional Gunung Gede Pangrango (TNGGP) sebagai Pemacu Pertumbuhan Tanaman dan Penghambat Phytophthora capsica (Undergraduate Thesis). Universitas Islam Negeri Syarif Hidayatullah. Retrieved from https://repository.uinjkt.ac.id/dspace/bitstream/123456789/77061/1/nadhira%20rasya%20salsabila-fst.pdf
Sari, P. M. (2019). Pengembangan Bahan Ajar Leaflet pada Materi Bangun Ruang Sisi Lengkung Siswa SMP (Undergraduate Thesis). UIN Raden Intan Lampung. Retrieved from https://repository.radenintan.ac.id/7592/
Sari, Y., Sulaiman, S., & Vitoria, L. (2023). Penggunaan Media Leafleat Terhadap Kreativitas Belajar Peserta Didik pada Pembelajaran PKn di Kelas V SDN 70 Banda Aceh. Elementary Education Research, 8(4), 338–349. Retrieved from https://jim.usk.ac.id/pgsd/article/download/24754/12652
Shakila, S., Taufik, A. N., & Nestiadi, A. (2024). Pengembangan E-Leaflet untuk Menumbuhkan Minat Belajar Siswa SMP Kelas VIII pada Tema Selamatkan Pernapasanku dari Asap Rokok. Jurnal Pendidikan MIPA, 14(4), 1001-1011. https://doi.org/10.37630/jpm.v14i4.2071
Sufa, M. R. W. (2023). Pengembangan Media Pembelajaran PowerPoint Interaktif untuk Meningkatkan Motivasi Belajar Siswa Kelas III pada Pembelajaran Tematik di SD Plus Al-Kautsar Kota Malang (Undergraduate Thesis). Universitas Islam Negeri Maulana Malik Ibrahim. Retrieved from http://etheses.uin-malang.ac.id/54772/
Susana, D. (2017). Pengaruh Penggunaan Bahan Ajar Leaflet Terhadap Penguasaan Materi Biologi Siswa Kelas X SMA Negeri 16 Bandar Lampung Tahun Pelajaran 2016/2017 (Undergraduate Thesis). UIN Raden Intan Lampung. Retrieved from https://repository.radenintan.ac.id/645/
Thiagarajan, S. (1974). Instructional Development for Training Teachers of Exceptional Children: A Sourcebook. Retrieved from https://eric.ed.gov/?id=ED090725
Utomo, E. P. (2018). Pengembangan LKPD Berbasis Komik untuk Meningkatkan Literasi Ekonomi Peserta Didik. Jurnal Penelitian Pendidikan, 35(1), 1– 9. https://doi.org/10.15294/jpp.v35i1.14015
Wahab, A., Junaedi, J., & Azhar, M. (2021). Efektivitas Pembelajaran Statistika Pendidikan Menggunakan Uji Peningkatan N-Gain di PGMI. Jurnal Basicedu, 5(2) 1039–1045. https://doi.org/10.31004/basicedu.v5i2.845
Waruwu, M. (2024). Metode Penelitian dan Pengembangan (R&D): Konsep, Jenis, Tahapan dan Kelebihan. Jurnal Ilmiah Profesi Pendidikan, 9(2), 1220-1230. https://doi.org/10.29303/jipp.v9i2.2141
Winarso, W., & Yuliyanti, D. D. (2017). Pengembangan Bahan Ajar Matematika Berbentuk Leaflet Berbasis Kemampuan Kognitif Siswa Berdasarkan Teori Bruner. JIPM (Jurnal Ilmiah Pendidikan Matematika), 6(1), 11. https://doi.org/10.25273/jipm.v6i1.1287
Author Biographies
Fitriya Ayu Wulandari, PGRI Argopuro University
Dwi Sucianingtyas Sukamto, PGRI Argopuro University
Hanif Rafika Putri, PGRI Argopuro University
License
Copyright (c) 2025 Fitriya Ayu Wulandari, Dwi Sucianingtyas Sukamto, Hanif Rafika Putri

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).