Vol. 11 No. 7 (2025): July
Open Access
Peer Reviewed

The Effect of NaCl Content Reduces Diarrhea Problems as Well as the Efficacy of Citrus Aurantifolia in It

Authors

Srinatalia Silaen , Cheryl Grace Pratiwi Rumahorbo

DOI:

10.29303/jppipa.v11i7.11246

Published:

2025-07-25

Downloads

Abstract

Lime peel contains saponins, flavonoids, alkaloids, tannins, and essential oils that have antibacterial activity. This study aims to explain the relationship between the use of lime peel as a traditional medicine and its bioactivity. Antibacterial activity testing used a Completely Randomized Design (CRD) spray method. The concentrations of lime peel extract used were 12.5, 25, 37.5, and 50%. The positive control used ciprofloxacin and the negative control was 10% DMSO. The results obtained were analyzed by Quasi-Experimental test. The use of plants as traditional medicine is related to their bioactivity and secondary metabolite content. Various researchers have successfully proven the bioactivity of lime peel, namely anticholesterol, antimicrobial, antioxidant, antihypertensive, antiosteoporosis, and anticancer. The concentration and length of contact time are directly proportional to the inhibitory power of CA water against Staphylococcus aureus bacteria. The bioactivity of CA extract as an antimicrobial is influenced by various factors, including the type of microbe, concentration, and contact time. This new study shows that lime peel has the benefit of killing harmful bacteria, such as Escherichia coli, which causes diarrhea. The vitamin C and antioxidants in lime peel are believed to be able to ward off these bacteria.

Keywords:

Citrus aurantifolia Diare NaCL Reduces diarrhea problem

References

Becerril-Sánchez, A. L., Quintero-Salazar, B., Dublán-García, O., & Escalona-Buendía, H. B. (2021). Phenolic Compounds in Honey and Their Relationship with Antioxidant Activity, Botanical Origin, and Color. Antioxidants, 10(11), 1700. https://doi.org/10.3390/antiox10111700

Bešlo, D., Golubić, N., Rastija, V., Agić, D., Karnaš, M., Šubarić, D., & Lučić, B. (2023). Antioxidant Activity, Metabolism, and Bioavailability of Polyphenols in the Diet of Animals. Antioxidants, 12(6), 1141. https://doi.org/10.3390/antiox12061141

Chassagne, F., Butaud, J.-F., Torrente, F., Conte, E., Ho, R., & Raharivelomanana, P. (2022). Polynesian Medicine Used to Treat Diarrhea and Ciguatera: An Ethnobotanical Survey in Six Islands from French Polynesia. Journal of Ethnopharmacology, 292, 115186. https://doi.org/10.1016/j.jep.2022.115186

Chen, H., Zeng, J., Wang, B., Cheng, Z., Xu, J., Gao, W., & Chen, K. (2021). Structural Characterization and Antioxidant Activities of Bletilla striata Polysaccharide Extracted by Different Methods. Carbohydrate Polymers, 266, 118149. https://doi.org/10.1016/j.carbpol.2021.118149

Christodoulou, M. C., Palacios, J. C. O., Hesami, G., Jafarzadeh, S., Lorenzo, J. M., Domínguez, R., Moreno, A., & Hadidi, M. (2022). Spectrophotometric Methods for Measurement of Antioxidant Activity in Food and Pharmaceuticals. Antioxidants, 11(11), 2213. https://doi.org/10.3390/antiox11112213

Cui, R., Zhang, C., Pan, Z., Hu, T., & Wu, H. (2024). Probiotic‐Fermented Edible Herbs as Functional Foods: A Review of Current Status, Challenges, and Strategies. Comprehensive Reviews in Food Science and Food Safety, 23(2), e13305. https://doi.org/10.1111/1541-4337.13305

Elachouri, M., Chaachouay, N., Zidane, L., Ouasti, I., & Bussmann, R. W. (2023). Citrus × Aurantium L.Citrus × sinensis (L.) OsbeckRutaceae. In Ethnobotany of Northern Africa and Levant (bll 1–13). Springer. https://doi.org/10.1007/978-3-031-13933-8_78-1

Gupta, S., Rahman, M. A., & Sundaram, S. (2021). Citrus Fruit as a Potential Source of Phytochemical, Antioxidant and Pharmacological Ingredients. JSHE, 2581, 8473. http://dx.doi.org/10.2019/JSHE.2581.8473/202101001

Hafeez, A., Ahmad, I., Naz, S., Alonaizan, R., Al-Akeel, R. K., Khan, R. U., & Tufarelli, V. (2023). Effect of Lemon (Citrus limon, L.) Peel Powder on Oocyst Shedding, Intestinal Health, and Performance of Broilers Exposed to E. tenella Challenge. Animals, 13(22), 3533. https://doi.org/10.3390/ani13223533

Ishaq, A. N., Sani, D., Abdullahi, S. A., Jolayemi, K. O., Ebbo, A. A., Jatau, I. D., & Gadzama, I. M. K. (2022). Evaluation of Anticoccidial Activity of Citrus aurantium L. Ethanolic Leaf Extract Against Experimental Eimeria tenella Infection in Broiler Chickens (Gallus gallus domesticus). Pharmacological Research - Modern Chinese Medicine, 4, 100138. https://doi.org/10.1016/j.prmcm.2022.100138

Karthikeyan, V., & Karthikeyan, J. (2014). Citrus aurantium (Bitter Orange): A Review of Its Traditional Uses, Phytochemistry and Pharmacology. International Journal of Drug Discovery and Herbal Research (IJDDHR), 4(4), 766-772. Retrieved from https://www.researchgate.net/publication/304404659

Komolafe, K., Komolafe, T., Crown, O., Ajiboye, B., Noubissi, F., Ogungbe, I., & Graham, B. (2025). Natural Products in the Management of Gastroesophageal Reflux Disease: Mechanisms, Efficacy, and Future Directions. Nutrients, 17(6), 1069. https://doi.org/10.3390/nu17061069

Liu, N., Yang, W., Li, X., Zhao, P., Liu, Y., Guo, L., Huang, L., & Gao, W. (2022). Comparison of Characterization and Antioxidant Activity of Different Citrus Peel Pectins. Food Chemistry, 386, 132683. https://doi.org/10.1016/j.foodchem.2022.132683

Liu, Y., Li, X., Chen, C., Leng, A., & Qu, J. (2021). Effect of Mineral Excipients on Processing Traditional Chinese Medicines: An Insight into the Components, Pharmacodynamics and Mechanism. Chinese Medicine, 16(1), 143. https://doi.org/10.1186/s13020-021-00554-8

Lu, X., Gu, X., & Shi, Y. (2022). A Review on Lignin Antioxidants: Their Sources, Isolations, Antioxidant Activities and Various Applications. International Journal of Biological Macromolecules, 210, 716–741. https://doi.org/10.1016/j.ijbiomac.2022.04.228

Lubis, N. D. A., Amelia, S., Yusraini, E., Rahmi, Z., & Balatif, R. (2023). Comparison of Antimicrobial Effectiveness of Orange and Lime’s Extracts on Aeromonas sobria and Aeromonas hydrophila. J Pure Appl Microbiol., 17(4), 2431-2436. https://doi.org/10.22207/JPAM.17.4.38

Muflihah, Y. M., Gollavelli, G., & Ling, Y.-C. (2021). Correlation Study of Antioxidant Activity with Phenolic and Flavonoid Compounds in 12 Indonesian Indigenous Herbs. Antioxidants, 10(10), 1530. https://doi.org/10.3390/antiox10101530

Munteanu, I. G., & Apetrei, C. (2021). Analytical Methods Used in Determining Antioxidant Activity: A Review. International Journal of Molecular Sciences, 22(7), 3380. https://doi.org/10.3390/ijms22073380

Ojha, S., Pandey, A. K., & Singh, P. (2023). Citrus aurantifolia Essential Oil Composition, Bioactivity, and Antibacterial Mode of Action on Salmonella Enterica, a Foodborne Pathogen. Food Science and Engineering, 75–88. https://doi.org/10.37256/fse.4120231615

Paniagua-Zambrana, N. Y., Bussmann, R. W., & Kikvidze, Z. (2024). Citrus medica L. Citrus sinensis (L.) Osbeck Rutaceae. In Ethnobotany of the Mountain Regions of Eastern Europe: Carpathians (bll 1–19). Springer. https://doi.org/10.1007/978-3-030-98744-2_83-1

Puri, V., Nagpal, M., Singh, I., Singh, M., Dhingra, G. A., Huanbutta, K., Dheer, D., Sharma, A., & Sangnim, T. (2022). A Comprehensive Review on Nutraceuticals: Therapy Support and Formulation Challenges. Nutrients, 14(21), 4637. https://doi.org/10.3390/nu14214637

Shaik, M. I., Hamdi, I. H., & Sarbon, N. M. (2023). A Comprehensive Review on Traditional Herbal Drinks: Physicochemical, Phytochemicals and Pharmacology Properties. Food Chemistry Advances, 3(2), 100460. https://doi.org/10.1016/j.focha.2023.100460

Shen, N., Wang, T., Gan, Q., Liu, S., Wang, L., & Jin, B. (2022). Plant Flavonoids: Classification, Distribution, Biosynthesis, and Antioxidant Activity. Food Chemistry, 383, 132531. https://doi.org/10.1016/j.foodchem.2022.132531

Siti, H. N., Mohamed, S., & Kamisah, Y. (2022). Potential Therapeutic Effects of Citrus hystrix DC and Its Bioactive Compounds on Metabolic Disorders. Pharmaceuticals, 15(2), 167. https://doi.org/10.3390/ph15020167

Stobiecka, M., Król, J., & Brodziak, A. (2022). Antioxidant Activity of Milk and Dairy Products. Animals, 12(3), 245. https://doi.org/10.3390/ani12030245

Sun, X., Sarteshnizi, R. A., & Udenigwe, C. C. (2022). Recent Advances in Protein–Polyphenol Interactions Focusing on Structural Properties Related to Antioxidant Activities. Current Opinion in Food Science, 45, 100840. https://doi.org/10.1016/j.cofs.2022.100840

Ugwuoke, G. M., Obi, C. F., Onu, I. J., & Idika, I. K. (2024). Therapeutic Efficacy of Citrus aurantifolia (Lime) Juice in Experimental Eimeria Tenella–Infected Broiler Chickens. Tropical Animal Health and Production, 56(1), 8. https://doi.org/10.1007/s11250-023-03840-9

Yu, S., Long, Y., Li, D., Shi, A., Deng, J., Ma, Y., Wen, J., Li, X., Zhang, Y., Liu, S., Wan, J., Li, N., & Guo, J. (2022). Natural Essential Oils Efficacious in Internal Organs Fibrosis Treatment: Mechanisms of Action and Application Perspectives. Pharmacological Research, 182, 106339. https://doi.org/10.1016/j.phrs.2022.106339

Zhu, Q., Pan, Z., Li, Z., Ye, R., Zhuang, Y., Yang, M., Wang, W., Pan, J., & Gao, Q. (2025). Large Chengqi Decoction Improves Sepsis-Related Intestinal Damage by Inhibiting Inflammatory Response Through the HMGB1-TLR4 Signaling Pathway. Journal of Inflammation Research, 18, 5415–5425. https://doi.org/10.2147/JIR.S490679

Zou, T., Wang, J., Wu, X., Yang, K., Zhang, Q., Wang, C., Wang, X., & Zhao, C. (2023). A Review of the Research Progress on Pinellia ternata (Thunb.) Breit.: Botany, Traditional Uses, Phytochemistry, Pharmacology, Toxicity and Quality Control. Heliyon, 9(11), e22153. https://doi.org/10.1016/j.heliyon.2023.e2215

Author Biographies

Srinatalia Silaen, Universitas HKBP Nommensen Medan

Author Origin : Indonesia

Cheryl Grace Pratiwi Rumahorbo, Universitas HKBP Nommensen Medan

Author Origin : Indonesia

Downloads

Download data is not yet available.

How to Cite

Silaen, S., & Rumahorbo, C. G. P. (2025). The Effect of NaCl Content Reduces Diarrhea Problems as Well as the Efficacy of Citrus Aurantifolia in It. Jurnal Penelitian Pendidikan IPA, 11(7), 258–262. https://doi.org/10.29303/jppipa.v11i7.11246