Weed Community Structure Enhances the Persistence of Entomopathogenic Fungi in Peri-Urban Rice Bund Soils
DOI:
10.29303/jppipa.v11i12.11490Published:
2025-09-30Downloads
Abstract
The persistence of entomopathogenic fungi (EPF) in agricultural soils is influenced by aboveground vegetation, yet the role of weed community structure remains underexplored. This study evaluated how weed cover, diversity, and abundance affect EPF persistence in bund soils of a peri-urban rice agroecosystem in Mulyoagung, Indonesia. Twenty-five bunds (five replicates per bund type) were sampled using quadrat-based vegetation surveys (125 quadrats total), and composite soil samples were collected for EPF isolation. EPF persistence was assessed using two indicators: (1) colony-forming unit (CFU) counts on selective medium, representing the abundance of fungal propagules regardless of taxonomic identity, and (2) Tenebrio molitor larval bioassays to measure infection potential. Vegetation variables were analyzed using Spearman correlation and Generalized Linear Models (GLMs). Weed cover was significantly associated with higher CFU counts (P = 0.008), and weed diversity was positively correlated with infection rates (P = 0.038); weed abundance showed no significant effect. These findings indicate that complex and diverse weed communities improve microhabitat conditions favorable to EPF survival and pathogenicity. Promoting weed canopy structure and species diversity on bunds may enhance EPF persistence and contribute to sustainable biological control in peri-urban rice farming systems
Keywords:
Entomopathogenic fungi Rice bunds Weed diversityReferences
Ames, H., Glenton, C., & Lewin, S. (2019). Purposive sampling in a qualitative evidence synthesis: A worked example from a synthesis on parental perceptions of vaccination communication. BMC Medical Research Methodology, 19(1), 1–9. https://doi.org/10.1186/s12874-019-0665-4 DOI: https://doi.org/10.1186/s12874-019-0665-4
Auld, B. (2009). Guidelines for monitoring weed control and recovery of native vegetation (1st ed.). NSW Department of Primary Industries.
Baverstock, J., Clark, S. J., & Pell, J. K. (2008). Effect of seasonal abiotic conditions and field margin habitat on the activity of Pandora neoaphidis inoculum on soil. Journal of Invertebrate Pathology, 97(3), 282–290. https://doi.org/10.1016/j.jip.2007.09.004 DOI: https://doi.org/10.1016/j.jip.2007.09.004
BMKG. (2025). Data Online BMKG: Beranda. Badan Meteorologi, Klimatologi, dan Geofisika (BMKG). https://dataonline.bmkg.go.id/beranda
Bottrell, D. G., & Weil, R. R. (1995). Protecting crops and the environment: Striving for durability. In Agriculture and the Environment (Vol. 60). Wiley. https://acsess.onlinelibrary.wiley.com/doi/abs/10.2134/asaspecpub60.c4 DOI: https://doi.org/10.2134/asaspecpub60.c4
Ding, J. L., Lin, H. Y., Hou, J., Feng, M. G., & Ying, S. H. (2023). The Entomopathogenic Fungus Beauveria bassiana Employs Autophagy as a Persistence and Recovery Mechanism during Conidial Dormancy. MBio, 14(2). https://doi.org/10.1128/mbio.03049-22 DOI: https://doi.org/10.1128/mbio.03049-22
Du, S., Trivedi, P., Wei, Z., Feng, J., Hu, H.-W., Bi, L., Huang, Q., & Liu, Y.-R. (2022). The Proportion of Soil-Borne Fungal Pathogens Increases with Elevated Organic Carbon in Agricultural Soils. MSystems, 7(2). https://doi.org/10.1128/msystems.01337-21 DOI: https://doi.org/10.1128/msystems.01337-21
García-Cela, M. E., Marín, S., Reyes, M., Sanchis, V., & Ramos, A. J. (2016). Conidia survival of Aspergillus section Nigri, Flavi and Circumdati under UV-A and UV-B radiation with cycling temperature/light regime. Journal of the Science of Food and Agriculture, 96(6), 2249–2256. https://doi.org/10.1002/jsfa.7343 DOI: https://doi.org/10.1002/jsfa.7343
Gemini. (2024). Peta Lokasi Penelitian Interaktif: Desa Mulyoagung, Dau, Malang. Google.
Gideon, P. E., Sugumar, R., & David, D. C. (2017). An in vitro study of antibacterial and antifungal activity of Cynodon dactylon. National Journal of Physiology, Pharmacy and Pharmacology, 7(4), 381–385. https://doi.org/10.5455/njppp.2017.7.1131912122016 DOI: https://doi.org/10.5455/njppp.2017.7.1131912122016
Hallouti, A., Ait Hamza, M., Zahidi, A., Ait Hammou, R., Bouharroud, R., Ait Ben Aoumar, A., & Boubaker, H. (2020). Diversity of entomopathogenic fungi associated with Mediterranean fruit fly (Ceratitis capitata (Diptera: Tephritidae)) in Moroccan Argan forests and nearby area: impact of soil factors on their distribution. BMC Ecology, 20(1), 1–13. https://doi.org/10.1186/s12898-020-00334-2 DOI: https://doi.org/10.1186/s12898-020-00334-2
Head, J. R., Sondermeyer-Cooksey, G., Heaney, A. K., Yu, A. T., Jones, I., Bhattachan, A., Campo, S. K., Wagner, R., Mgbara, W., Phillips, S., Keeney, N., Taylor, J., Eisen, E., Lettenmaier, D. P., Hubbard, A., Okin, G. S., Vugia, D. J., Jain, S., & Remais, J. V. (2022). Effects of precipitation, heat, and drought on incidence and expansion of coccidioidomycosis in western USA: a longitudinal surveillance study. The Lancet Planetary Health, 6(10), e793–e803. https://doi.org/10.1016/S2542-5196(22)00202-9 DOI: https://doi.org/10.1016/S2542-5196(22)00202-9
Islam, A. K. M. M., Uddin, M. N., Yeasmin, S., Kheya, S. A., Islam, M. S., Ahmed, S., Hossain, A., & Anwar, M. P. (2023). Preliminary report on the comparative weed suppressibility of Bangladeshi Wheat varieties. Heliyon, 9(4), e14942. https://doi.org/10.1016/j.heliyon.2023.e14942 DOI: https://doi.org/10.1016/j.heliyon.2023.e14942
Iwanicki, N. S. A., Castro, T., Eilenberg, J., Meyling, N. V., Moral, R. de A., Demétrio, C. G. B., & Delalibera, I. (2024). Community composition of the entomopathogenic fungal genus Metarhizium in soils of tropical and temperate conventional and organic strawberry fields. Journal of Invertebrate Pathology, 204(August 2023). https://doi.org/10.1016/j.jip.2024.108079 DOI: https://doi.org/10.1016/j.jip.2024.108079
Khalid, M., ur Rahman, S., Liu, X., Rehman, A., Jumpponen, A., Johan Kotze, D., Setälä, H., & Hui, N. (2024). Exploring the impact of urbanization and vegetation type on fungal communities: Insights into divergent, mycorrhizal, and saprophytic associations driven by climate patterns. Catena, 238. https://doi.org/10.1016/j.catena.2024.107860 DOI: https://doi.org/10.1016/j.catena.2024.107860
Li, Y., Wang, J., Shen, C., Wang, J., Singh, B. K., & Ge, Y. (2022). Plant diversity improves resistance of plant biomass and soil microbial communities to drought. Journal of Ecology, 110(7), 1656–1672. https://doi.org/10.1111/1365-2745.13900 DOI: https://doi.org/10.1111/1365-2745.13900
Lopez-Llorca, L. V, & Olivares-Bernabeu, C. (1997). Growth inhibition of nematophagous and entomopathogenic fungi by leaf litter and soil containing phenols. Mycological Research, 101(5), 691–697. https://doi.org/10.1017/S0953756296003176 DOI: https://doi.org/10.1017/S0953756296003188
Lubis, W., Umami Harahap, W., & Nurhajijah. (2022). Identifikasi Jenis Dan Nilai Summed Dominance Ratio (Sdr) Gulma Di Lahan Kering. RADIKULA: Jurnal Ilmu Pertanian, 1(1), 20–25. https://doi.org/10.33379/radikula.v1i1.1215 DOI: https://doi.org/10.33379/radikula.v1i1.1215
Miranda-Fuentes, P., García-Carneros, A. B., Montilla-Carmona, A. M., & Molinero-Ruiz, L. (2020). Evidence of soil-located competition as the cause of the reduction of sunflower verticillium wilt by entomopathogenic fungi. Plant Pathology, 69(8), 1492–1503. https://doi.org/10.1111/ppa.13230 DOI: https://doi.org/10.1111/ppa.13230
Naidu, V. S. G. . (2012). Hand Book on Weed Identification. In Directorate of Weed Science Research. Directorate of Weed Science Research.
Pérez-Méndez, N., Alcaraz, C., & Catala-Forner, M. (2025). Ecological restoration of field margins enhances biodiversity and multiple ecosystem services in rice agroecosystems. Agriculture, Ecosystems and Environment, 382(January). https://doi.org/10.1016/j.agee.2025.109484 DOI: https://doi.org/10.1016/j.agee.2025.109484
PlantNet. (2025). PlantNet: Plant Identification and Information. PlantNet. https://plantnet.org/en/
Posadas, J. B., Comerio, R. M., Mini, J. I., Nussenbaum, A. L., & Lecuona, R. E. (2012). A novel dodine-free selective medium based on the use of cetyl trimethyl ammonium bromide (CTAB) to isolate Beauveria bassiana, Metarhizium anisopliae sensu lato and paecilomyces lilacinus from soil. Mycologia, 104(4), 974–980. https://doi.org/10.3852/11-234 DOI: https://doi.org/10.3852/11-234
Sari, R. W. S. W. S., & Yuliani, E. (2022). Identifikasi Dampak Alih Fungsi Lahan Pertanian Ke Non Pertanian Untuk Perumahan. Jurnal Kajian Ruang, 1(2), 255. https://doi.org/10.30659/jkr.v1i2.20032 DOI: https://doi.org/10.30659/jkr.v1i2.20032
Sharma, L., Oliveira, I., Gonçalves, F., Raimundo, F., Singh, R. K., Torres, L., & Marques, G. (2021). Effect of soil chemical properties on the occurrence and distribution of entomopathogenic fungi in Portuguese grapevine fields. Pathogens, 10(2), 1–13. https://doi.org/10.3390/pathogens10020137 DOI: https://doi.org/10.3390/pathogens10020137
Strasser, H., Forer, A., & Schinner, F. (1996). Development of media for the selective isolation and maintenance of virulence of Beauveria brongniartii. Proceedings 3rd International Workshop on Microbial Control of Soil Dwelling Pests 3rd International Workshop on Microbial Control of Soil Dwelling Pests, 125–130.
Sutanto, K. D., Husain, M., Rasool, K. G., Malik, A. F., Al-Qahtani, W. H., & Aldawood, A. S. (2022). Persistency of Indigenous and Exotic Entomopathogenic Fungi Isolates under Ultraviolet B (UV-B) Irradiation to Enhance Field Application Efficacy and Obtain Sustainable Control of the Red Palm Weevil. Insects, 13(1). https://doi.org/10.3390/insects13010103 DOI: https://doi.org/10.3390/insects13010103
Swathy, K., Vivekanandhan, P., Yuvaraj, A., Sarayut, P., Kim, J. S., & Krutmuang, P. (2024). Biodegradation of pesticide in agricultural soil employing entomopathogenic fungi: Current state of the art and future perspectives. Heliyon, 10(1), e23406. https://doi.org/10.1016/j.heliyon.2023.e23406 DOI: https://doi.org/10.1016/j.heliyon.2023.e23406
Team RStudio. (2024). RStudio: Integrated Development Environment for R. Posit, PBC. https://posit.co/
Tjitrosoedirdjo, S., Utomo, I. H., & Wiroatmodjo, J. (1984). Pengelolaan gulma di perkebunan. Gramedia.
Tkaczuk, C., Krzyczkowski, T., & Wegensteiner, R. (2013). The occurrence of entomopathogenic fungi in soils from mid-field woodlots and adjacent small-scale arable fields. Acta Mycologica, 47(2), 191–202. https://doi.org/10.5586/am.2012.024 DOI: https://doi.org/10.5586/am.2012.024
Tuão Gava, C. A., da Silva, J. C., Simões, W. L., & Jordão Paranhos, B. A. (2021). Impact of soil texture on conidia movement and residual effect of entomopathogenic fungi applied through irrigation to control fruit-fly pupae in mango orchards. Biological Control, 163(February). https://doi.org/10.1016/j.biocontrol.2021.104559 DOI: https://doi.org/10.1016/j.biocontrol.2021.104559
Vaicekonyte, R. (2011). The effects of the removal of garlic mustard (Alliaria petiolata) on the abundance of entomopathogenic fungi. Invasive Plant Science and Management. https://www.academia.edu/download/53410398/The_Effects_of_the_Removal_of_Garlic_Mus20170606-8625-1enw35.pdf
Vukicevich, E., Thomas Lowery, D., Bennett, J. A., & Hart, M. (2019). Influence of groundcover vegetation, soil physicochemical properties, and irrigation practices on soil fungi in semi-arid vineyards. Frontiers in Ecology and Evolution, 7(APR), 1–10. https://doi.org/10.3389/fevo.2019.00118 DOI: https://doi.org/10.3389/fevo.2019.00118
Wang, L., Li, Q., li, C., Wu, C., Chen, F., Chen, X., & Zhang, F. (2023). Nitrate Nitrogen and pH Correlate with Changes in Rhizosphere Microbial Community Assemblages during Invasion of Ambrosia artemisiifolia and Bidens pilosa. Microbiology Spectrum, 11(1). https://doi.org/10.1128/spectrum.03649-22 DOI: https://doi.org/10.1128/spectrum.03649-22
Wang, X., Ma, J., & Wang, D. (2025). Understanding the risks of peri-urbanization to food systems to help establish sustainable agriculture near cities. Environmental Impact Assessment Review, 112, 107777. https://doi.org/10.1016/j.eiar.2024.107777 DOI: https://doi.org/10.1016/j.eiar.2024.107777
Yang, H., Qin, C. S., Chen, Y. M., Zhang, G. Y., Dong, L. H., & Wan, S. Q. (2019). Persistence of Metarhizium (Hypocreales: Clavicipitaceae) and Beauveria bassiana (Hypocreales: Clavicipitaceae) in Tobacco Soils and Potential as Biocontrol Agents of Spodoptera litura (Lepidoptera: Noctuidae). Environmental Entomology, 48(1), 147–155. https://doi.org/10.1093/ee/nvy161 DOI: https://doi.org/10.1093/ee/nvy161
Zambelli, A., Nocito, F. F., & Araniti, F. (2025). Unveiling the multifaceted roles of root exudates: Chemical interactions, allelopathy, and agricultural applications. Agronomy, 15, 845. https://doi.org/https://doi.org/ 10.3390/agronomy15040845 DOI: https://doi.org/10.3390/agronomy15040845
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