Integrating Experimental Evaluation and Bibliometric Analysis of Photosynthetic Bacteria and Jakaba as Bioactivators for Sustainable Organic Agriculture
DOI:
10.29303/jppipa.v11i7.11789Published:
2025-07-25Downloads
Abstract
The excessive use of synthetic chemical inputs in agriculture has led to soil degradation, highlighting the need for environmentally friendly bioactivator alternatives. This study aims to integrate bibliometric analysis and experimental evaluation of Photosynthetic Bacteria (PSB) and Jakaba (Corallomycetella repens) as bioactivators in organic farming systems. Bibliometric analysis was conducted using Scopus publications from 2015–2025 and visualized with VOSviewer to map research trends, dominant keywords, and the publication status of PSB and Jakaba. A field experiment was then carried out in Kaluku Tinggu Village, Sigi District, using hybrid corn (Bisi-18) with four treatments: control, PSB, Jakaba, and a combination of PSB+Jakaba. Data were analyzed using ANOVA and Tukey HSD test, supported by Response Surface Methodology (RSM) for treatment effectiveness evaluation. Results showed that the PSB+Jakaba combination increased plant height (15.3%), fresh weight (20.9%), dry weight (25.1%), soil pH (13.5%), organic C content (41.2%), cation exchange capacity (27.6%), microbial population (24%), and dehydrogenase activity (36.3%) compared to the control. Active compounds such as IAA and siderophores from PSB, along with lignocellulolytic enzymes from Jakaba, played key roles in improving soil quality. This study bridges the scientific knowledge gap and reinforces the potential of local bioactivator formulations for sustainable organic farming on marginal land.
Keywords:
Biactivator Bibliometrics Jakaba Organic farming Photosynthetic bacteriaReferences
Abdelaal, K., Alkahtani, M., Attia, K., Hafez, Y., Király, L., & Künstler, A. (2021). The Role of Plant Growth-Promoting Bacteria in Alleviating the Adverse Effects of Drought on Plants. Biology, 10(6), 520. https://doi.org/10.3390/biology10060520
Abebe, T. G., Tamtam, M. R., Abebe, A. A., Abtemariam, K. A., Shigut, T. G., Dejen, Y. A., & Haile, E. G. (2022). Growing Use and Impacts of Chemical Fertilizers and Assessing Alternative Organic Fertilizer Sources in Ethiopia. Applied and Environmental Soil Science, 2022. https://doi.org/10.1155/2022/4738416
Altieri, M. A., & Nicholls, C. I. (2020). Agroecology and the emergence of a post COVID-19 agriculture. Agriculture and Human Values, 37(3), 525–526. https://doi.org/10.1007/s10460-020-10043-7
Altieri, M. A., & Toledo, V. M. (2011). The agroecological revolution in Latin America: Rescuing nature, ensuring food sovereignty and empowering peasants. Journal of Peasant Studies, 38(3), 587–612. https://doi.org/10.1080/03066150.2011.582947
Bala, S., Garg, D., Thirumalesh, B. V., Sharma, M., Sridhar, K., Inbaraj, B. S., & Tripathi, M. (2022). Recent Strategies for Bioremediation of Emerging Pollutants: A Review for a Green and Sustainable Environment. Toxics, 10(8), 1–24. https://doi.org/10.3390/toxics10080484
Bay, S. K., Waite, D. W., Dong, X., Gillor, O., Chown, S. L., Hugenholtz, P., & Greening, C. (2021). Chemosynthetic and photosynthetic bacteria contribute differentially to primary production across a steep desert aridity gradient. ISME Journal, 15(11), 3339–3356. https://doi.org/10.1038/s41396-021-01001-0
Brown, B., Wilkins, M., & Saha, R. (2022). Rhodopseudomonas palustris: A biotechnology chassis. Biotechnology Advances, 60(June), 108001. https://doi.org/10.1016/j.biotechadv.2022.108001
Cao, K., Zhi, R., & Zhang, G. (2020). Photosynthetic bacteria wastewater treatment with the production of value-added products: A review. Bioresource Technology, 299(December), 122648. https://doi.org/10.1016/j.biortech.2019.122648
Chen, Y., Shi, W., Aihemaitijiang, G., & Zhang, F. (2025). Hyperspectral inversion of heavy metal content in farmland soil under conservation tillage of black soils. Scientific Reports, 15, 354. https://doi.org/10.1038/s41598-024-83479-0
Dar, G. H. B., Bhat, R. A., Mehmood, M. A., & Hakeem, K. R. (2021). Microbiota and Biofertilizers (Vol 2). Springer. https://doi.org/10(1007), 978-3.
Donthu, N., Kumar, S., Mukherjee, D., Pandey, N., & Lim, W. M. (2021). How to conduct a bibliometric analysis: An overview and guidelines. Journal of Business Research, 133(May), 285–296. https://doi.org/10.1016/j.jbusres.2021.04.070
Fadilah, R. A., Kurnia, M. D., & Putra, I. P. (2024). Jakaba Undercover: Taxonomic Riddle and Potency in Indonesian Agriculture. Caraka Tani: Journal of Sustainable Agriculture, 39(2), 411–423. https://doi.org/10.20961/carakatani.v39i2.89049
Fahrudin, T. M. (2024). Bibliometric Analysis and Literature Review of Big Data Research Fields using Publish or Perish and VOSviewer. Nusantara Science and Technology Proceedings, 250–258. https://doi.org/10.11594/nstp.2024.4140
Fidelis, R. R., Cavazzini, P. H., & Burin, L. X. (2022). Phosphate fertilization and bioactivator influences on fractions of organic matter and soil microbial biomass. Research, Society and Development, 11(9), e52211932086-e52211932086. Retrieved from https://rsdjournal.org/index.php/rsd/article/view/32086
Fudjoe, S. K., Shi, S., Li, L., Anwar, S., Xie, J., Chimsah, F., & Wang, L. (2025). Gene expression through comparative transcriptome analysis unravels the molecular mechanisms of fertilizer-induced hormonal regulation and leaf senescence in maize for enhanced yield in semiarid regions. BMC Plant Biology, 25(1). https://doi.org/10.1186/s12870-025-06303-5
Gamage, A., Gangahagedara, R., Gamage, J., Jayasinghe, N., Kodikara, N., Suraweera, P., & Merah, O. (2023). Role of organic farming for achieving sustainability in agriculture. Farming System, 1(1), 100005. https://doi.org/10.1016/j.farsys.2023.100005
Hirawan, D., Oktafiani, D., Fauzan, T. A., Luckyardi, S., & Jamil, N. (2022). Research Trends in Farming System Soil Chemical: A Bibliometric Analysis using VOSviewer. Moroccan Journal of Chemistry, 10(3), 576–590. https://doi.org/10.48317/IMIST.PRSM/morjchem-v10i3.33145
Hitchcock, A., Hunter, C. N., Sobotka, R., Komenda, J., Dann, M., & Leister, D. (2022). Redesigning the photosynthetic light reactions to enhance photosynthesis – the PhotoRedesign consortium. Plant Journal, 109(1), 23–34. https://doi.org/10.1111/tpj.15552
Hsu, S. H., Shen, M. W., Chen, J. C., Lur, H. S., & Liu, C. Te. (2021). The Photosynthetic Bacterium Rhodopseudomonas palustris Strain PS3 Exerts Plant Growth-Promoting Effects by Stimulating Nitrogen Uptake and Elevating Auxin Levels in Expanding Leaves. Frontiers in Plant Science, 12(February), 1–18. https://doi.org/10.3389/fpls.2021.573634
Irwandhi, Kamaluddin, N. N., Khumairah, F. H., Prihatiningsih, N., & Simarmata, T. (2025). Assessment of local wisdom biofertilizer formulas on enhancing microbial diversity and photosynthate allocation in acid-stressed maize. Asian Journal of Agriculture, 9(1), 112–121. https://doi.org/10.13057/asianjagric/g090112
Kavamura, V. N., & Esposito, E. (2010). Biotechnological strategies applied to the decontamination of soils polluted with heavy metals. Biotechnology Advances, 28(1), 61–69. https://doi.org/10.1016/j.biotechadv.2009.09.002
Khan, A. R., Mustafa, A., Hyder, S., Valipour, M., Rizvi, Z. F., Gondal, A. S., Yousuf, Z., Iqbal, R., & Daraz, U. (2022). Bacillus spp. as Bioagents: Uses and Application for Sustainable Agriculture. Biology, 11(12), 1–21. https://doi.org/10.3390/biology11121763
Lee, S. K., Lur, H. S., & Liu, C. Te. (2021). From lab to farm: Elucidating the beneficial roles of photosynthetic bacteria in sustainable agriculture. Microorganisms, 9(12), 1–23. https://doi.org/10.3390/microorganisms9122453
Li, M., Ning, P., Sun, Y., Luo, J., & Yang, J. (2022). Characteristics and Application of Rhodopseudomonas palustris as a Microbial Cell Factory. Frontiers in Bioengineering and Biotechnology, 10(May), 1–19. https://doi.org/10.3389/fbioe.2022.897003
Manuelian, C. L., Penasa, M., da Costa, L., Burbi, S., Righi, F., & De Marchi, M. (2020). Organic livestock production: A bibliometric review. Animals, 10(4), 1–16. https://doi.org/10.3390/ani10040618
Mukherjee, D., Lim, W. M., Kumar, S., & Donthu, N. (2022). Guidelines for advancing theory and practice through bibliometric research. Journal of Business Research, 148(May), 101–115. https://doi.org/10.1016/j.jbusres.2022.04.042
Mutaqqim, S. Z. (2025). Fermentasi Pupuk Jamur Keberuntungan Abadi (Jakaba) Memanfaatkan Limbah Organik Rumah Tangga: Uji Pertumbuhan pada Tanaman Pakcoy. Retrieved from http://repository.uki.ac.id/id/eprint/18496
Nguyen Quoc, K., Vinh, T. Le, Thanh, Q. Le, Ngoc, H. T., Thi, X. Do, & Huu, D. H. (2023). Effects of biofertilizer supplementation, Rhodopseudomonas spp., on nitrogen and phosphorus uptakes, growth, and yield of sesame (Sesamum indicum L.) on salt-affected soil. Journal of Plant Nutrition, 47(1), 1–17. https://doi.org/10.1080/01904167.2023.2278646
Patthawaro, S., Lomthaisong, K., & Saejung, C. (2020). Bioconversion of Agro-Industrial Waste to Value-Added Product Lycopene by Photosynthetic Bacterium Rhodopseudomonas faecalis and Its Carotenoid Composition. Waste and Biomass Valorization, 11(6), 2375–2386. https://doi.org/10.1007/s12649-018-00571-z
Puspitasari, Y. D., Cahyanti, T. W., & Nugroho, P. A. (2022). Production Revitalization And Community Based Knowledge Improvement In Plant Breeding Using Abadi Luck Mushroom (Jakaba) To Increase Family Welfare. Prosiding Seminar Nasional UNIMUS, 5(3), 1994–2004. Retrieved from https://prosiding.unimus.ac.id/index.php/semnas/article/download/1172/1170
Rejeb, A., Abdollahi, A., Rejeb, K., & Treiblmaier, H. (2022). Drones in agriculture: A review and bibliometric analysis. Computers and Electronics in Agriculture, 198(December), 107017. https://doi.org/10.1016/j.compag.2022.107017
Sabki, M. H., Ong, P. Y., Lee, C. T., Ibrahim, N., van Fan, Y., & Klemeš, J. J. (2021). The Potential of Rhodopseudomonas Palustris as a Bio-Fertiliser for Sustainable Agriculture. Chemical Engineering Transactions, 88(May), 457–462. https://doi.org/10.3303/CET2188076
Sundar, L. S., Yen, K. S., Chang, Y. T., & Chao, Y. Y. (2024). Utilization of Rhodopseudomonas palustris in Crop Rotation Practice Boosts Rice Productivity and Soil Nutrient Dynamics. Agriculture (Switzerland), 14(5). https://doi.org/10.3390/agriculture14050758
Suzuki, M., Shirai, T., Morey-Yagi, S. R., Kondo, A., & Numata, K. (2025). Evaluation of nitrogen fixation in the marine purple photosynthetic bacterium Rhodovulum sulfidophilum under autotrophic and heterotrophic conditions. Scientific Reports, 15(1), 2–9. https://doi.org/10.1038/s41598-025-03605-4
Thakur, N., Kaur, S., Kaur, T., Tomar, P., Devi, R., & Thakur, S. (2022). Organic agriculture for agro-environmental sustainability. Trends of Applied Microbiology for Sustainable Economy, 699–735. https://doi.org/10.1016/B978-0-323-91595-3.00018-5
Vishnupradeep, R., Bruno, L. B., Taj, Z., Karthik, C., Challabathula, D., Tripti, Kumar, A., Freitas, H., & Rajkumar, M. (2022). Plant growth promoting bacteria improve growth and phytostabilization potential of Zea mays under chromium and drought stress by altering photosynthetic and antioxidant responses. Environmental Technology and Innovation, 25, 102154. https://doi.org/10.1016/j.eti.2021.102154
Wang, Y., Peng, S., Hua, Q., Qiu, C., Wu, P., Liu, X., & Lin, X. (2021). The Long-Term Effects of Using Phosphate-Solubilizing Bacteria and Photosynthetic Bacteria as Biofertilizers on Peanut Yield and Soil Bacteria Community. Frontiers in Microbiology, 12(July), 1–14. https://doi.org/10.3389/fmicb.2021.693535
Wei, J., Wu, J., Wei, Y., Yimiti, D., & Mi, X. (2023). Metabolic composition of endophytes contributed to the effect of Brassica rapa L. in improving immunity. Retrieved from https://www.researchsquare.com/article/rs-2954577/v1
Wikurendra, E. A., Nurika, G., Herdiani, N., & Lukiyono, Y. T. (2022). Evaluation of the Commercial Bio-Activator and a Traditional Bio-Activator on Compost Using Takakura Method. Journal of Ecological Engineering, 23(6), 278–285. https://doi.org/10.12911/22998993/149303
License
Copyright (c) 2025 Nanda, Rafiqah Amanda Lubis, Mukhlis, Fery Endang Nasution, Luky Wahyu Sipahutar
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
