Characterization of PVA-Enzyme Coated Indicator Electrodes GA coated again with PVC-KTpClPB-o-NPOE UV-Vis analysis, variable signal analysis, sensor sensitivity and SEM-EDS

Authors

Abd Hakim S

DOI:

10.29303/jppipa.v7iSpecialIssue.1248

Published:

2021-12-26

Issue:

Vol. 7 No. SpecialIssue (2021): December

Keywords:

UV-Visible and SEM-EDS, GA and o-NPOE, tungsten indicator electrode, Biosensor potentiometry, Immobilization

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S, A. H. (2021). Characterization of PVA-Enzyme Coated Indicator Electrodes GA coated again with PVC-KTpClPB-o-NPOE UV-Vis analysis, variable signal analysis, sensor sensitivity and SEM-EDS. Jurnal Penelitian Pendidikan IPA, 7(SpecialIssue), 370–376. https://doi.org/10.29303/jppipa.v7iSpecialIssue.1248
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Abstract

This study aims to characterize the phosphate buffer and urease enzymes through UV-Vis and SEM-EDS absorbance spectra using tungsten as an indicator electrode. The method used is a potentiometric biosensor with urease enzyme immobilization technique for urea analyte. A small detection range of 10-5-10-4M has been studied with PVA-enzyme coated indicator electrodes coated with PVC-KTpClPB. On this basis, the researchers increased the detection range by analyzing glutaraldehyde (GA) mixed with PVA-enzyme and o-NPOE mixed with PVC-KTpClPB. The best results of GA mixed PVA-enzyme at GA2.9% UV-Visible analysis. The best results were PVA-enzyme coated indicator electrodes coated with GA coated again with PVC-KTpClPB-o-NPOE SEM-EDS analysis on PVA-enzyme samples 3x coated with GA 1x and PVC-KTpClPB-o-NPOE 1x with o-NPOE variation of 61% and 66%.

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Author Biography

Abd Hakim S, Universitas Negeri Medan

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