Optimization of Wet Rendering and Alkali Neutralization for Omega-3-Rich Oil Production from Milkfish (Chanos chanos)
DOI:
10.29303/jppipa.v11i11.12538Published:
2025-11-25Downloads
Abstract
This study addresses the instability, off-flavors, and variable quality that limit the use of fish oil as an omega-3 source, particularly from milkfish (Chanos chanos). The research aims to produce omega-3-rich milkfish oil that meets international quality standards and is suitable for microencapsulation. Fresh milkfish was processed using wet rendering at four temperatures (70, 80, 90, and 100°C) and three heating times (30, 40, and 60 min). The crude oil was then refined by alkali neutralization with different NaOH concentrations. Oil yield and oxidative quality parameters—peroxide value, p-anisidine value, total oxidation (TOTOX), and acid value—were measured and analyzed using factorial ANOVA. The highest yield (0.29%) was obtained at 100°C for 60 min. Peroxide and anisidine values (3.44 and 11.75 meq/kg) and TOTOX (<20 meq/kg) complied with International Fish Oil Standards, whereas acid values (2.43–2.68 mg KOH/g) exceeded the limit at prolonged high-temperature extraction. These results indicate that higher temperatures increase oil recovery but promote hydrolytic degradation. Careful optimization of time and temperature is therefore required to obtain stable, high-quality omega-3 milkfish oil for further microencapsulation and application in health-oriented food products.
Keywords:
Alkali neutralization, Microencapsulation, Milkfish oil, Omega-3 fatty acids, Wet renderingReferences
Annamalai, J., & Abubacker, Z. A. (2020). Microencapsulation of Fish Oil Using Fish Protein Hydrolysate, Maltodextrin, and Gum Arabic : Effect on Structural and Oxidative Stability Microencapsulation of Fish Oil Using Fish Protein Hydrolysate. Journal of Aquatic Food Product Technology, 00(00), 1–14. https://doi.org/10.1080/10498850.2020.1723765
Arias, L., Marquez, D. M., & Zapata, E. (2022). Quality of red tilapia viscera oil (Oreochromis sp .) as a function of extraction methods. Heliyon, 8(5). https://doi.org/10.1016/j.heliyon.2022.e09546
Ayeloja, A., Jimoh, W., & Garuba, A. (2024). Nutritional quality of fish oil extracted from selected freshwater fish species. Food Chemistry Advances, 4(June), 100720. https://doi.org/10.1016/j.focha.2024.100720
Bannenberg, G., Mallon, C., Edwards, H., Yeadon, D., & Yan, K. (2017). Omega-3 Long-Chain Polyunsaturated Fatty Acid Content and Oxidation State of Fish Oil Supplements in New Zealand. Scientific Reports, 1–13. https://doi.org/10.1038/s41598-017-01470-4
Bija, S., Suseno, S. H., & Uju, U. (2017). Purification of sardine fish oil through degumming and neutralization. Jurnal Pengolahan Hasil Perikanan Indonesia, 20(1), 143-152. https://doi.org/10.17844/jphpi.v20i1.16501
Comitini, F., Peila, C., Fanos, V., & Coscia, A. (2020). The Docosahexanoic Acid : From the Maternal-Fetal Dyad to Early Life Toward Metabolomics. 8(September), 1–5. https://doi.org/10.3389/fped.2020.00538
Getahun, A., Kechero, Y., & Yemane, N. (2025). Review on fish production enhancement and preservation technologies. Veterinary Sciences: Research and Reviews, 11(1), 54-70. https://dx.doi.org/10.17582/journal.vsrr/2025/11.1.54.70
Hidayah, N., Rohman, A., Mustafidah, M., I. (2022). Physicochemical Properties and Antioxidant Activity of Fish Oil from Indian Mackerel (Rastrelliger kanagurta). Current Applied Science And Technology, 6(April), 265–270. https://doi.org/10.55003/cast.2025.265241
Hussain, S., Rahman, A., Borah, P., Sen, A., Bharalee, R., Mayuri, & Verma, A. K. (2025). Eco-friendly Advancements through Fish Waste: A Review of Therapeutic and Industrial Innovations. Combinatorial Chemistry & High Throughput Screening. https://doi.org/10.2174/0113862073372954250408181058
Khan, U., Lone, A. N., Shahzeb, M., Virani, S. S., Blumenthal, R. S., Nasir, K., Miller, M., Michos, E. D., Ballantyne, C. M., Boden, W. E., & Bhatt, D. L. (2021). EClinicalMedicine Effect of omega-3 fatty acids on cardiovascular outcomes : A systematic review and meta-analysis. 38. https://doi.org/10.1016/j.eclinm.2021.100997
Ningsih, S. W., Lubis, N. A., & Nasution, G. S. (2024). The Quality of Purified Eel Fish (Monopterus Albus Zuieuw) Oil and Mackerel Tuna Fish (Euthynnus Affinis) Oil. Health Notions, 8(1), 1-7. Retrieved from http://heanoti.com/index.php/hn/article/view/hn80101
Palabiyik, A. A., & Palabiyik, E. (2025). Effects of omega-3 fatty acids (n-3 PUFAs) on cardiovascular and muscular health across various populations: a review of supplementation benefits. Nutrire, 50(2), 73. https://doi.org/10.1186/s41110-025-00381-z
Perez-Palacios, T., Ruiz-Carrascal, J., Solomando, J. C., De-la-Haba, F., Pajuelo, A., & Antequera, T. (2022). Recent developments in the microencapsulation of fish oil and natural extracts: procedure, quality evaluation and food enrichment. Foods, 11(20), 3291. https://doi.org/10.3390/foods11203291
Purnamayati, L., Istianisa, W., Sumardianto and Suharto, S. (2023). Refining of tilapia ( Oreochromis niloticus ) viscera oil with different sodium hydroxide concentrations. Food Research 7(4), 106–113. https://doi.org/10.26656/fr.2017.7(4).816
Samarajeewa, U. (2024). Safety, processing, and utilization of fishery products. Fishes, 9(4), 146. https://doi.org/10.3390/fishes9040146
Santos, H. O., Price, J. C., & Bueno, A. A. (2020). Beyond fish oil supplementation: the effects of alternative plant sources of omega-3 polyunsaturated fatty acids upon lipid indexes and cardiometabolic biomarkers—an overview. Nutrients, 12(10), 3159. https://doi.org/10.3390/nu12103159
Sasongko, H., Indah, I., Nurrochmad, A., Nugroho, A. E., & Rohman, A. (2025). Effects of Wet Rendering Extraction on the Fatty Acid and Physicochemicalprofiles of Catfish (Pangasius Micronema Blkr.), Milkfish (Chanos chanosForsskal.) and Snakehead Fish (Chana Striata Bloch). Food Research, 9(1), 13-18. https://doi.org/10.26656/fr.2017.9(1).518
Swetha, N., & Mathanghi, S. K. (2024). Towards sustainable omega-3 fatty acids production–A comprehensive review on extraction methods, oxidative stability and bio-availability enhancement. Food Chemistry Advances, 4, 100603. https://doi.org/10.1016/j.focha.2023.100603
Visioli, F., & Poli, A. (2020). Fatty acids and cardiovascular risk. Evidence, lack of evidence, and diligence. Nutrients, 12(12), 3782. https://doi.org/10.3390/nu12123782
Yi, M., You, Y., Zhang, Y., Wu, G., Karrar, E., Zhang, L., & Wang, X. (2023). Highly valuable fish oil: Formation process, enrichment, subsequent utilization, and storage of eicosapentaenoic acid ethyl esters. Molecules, 28(2), 672. https://doi.org/10.3390/molecules28020672
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