Identification and Characterisation of Entomopathogenic Microbes: An Innovative Science Learning Resource to Improve Student Science Literacy on the Topic of Agricultural Biotechnology
DOI:
10.29303/jppipa.v11i10.13173Published:
2025-10-31Downloads
Abstract
The challenge in science education is to connect biotechnology concepts with real-world contexts and local potential. This study aims to explore and identify local entomopathogenic microbes as innovative learning resources on the topic of agricultural biotechnology, specifically biological control of onion pests. This exploratory study includes field surveys in the onion centre of Guntarano Village and laboratory analysis. Isolation of entomopathogenic fungi from infected Liriomyza spp. larvae was carried out using the insect bait method. The isolates obtained were purified, identified based on morphological characteristics (macroscopic and microscopic), and their spore density was calculated. The exploration successfully screened six potential fungal isolates. Morphological identification revealed the presence of Aspergillus flavus, Aspergillus niger, Trichoderma sp., and one isolate suspected to be Metarhizium sp. All isolates had high spore densities (> 10⁷ conidia/mL), with the Metarhizium sp. (Larva 4) and A. niger (Larva 2) isolates showing the highest densities. The diversity of entomopathogenic microbes found has the potential to be a source of biopesticide learning in science education. This material can be utilised to develop modules, worksheets, or learning media based on local potential to enhance science literacy and students' understanding of agricultural biotechnology
Keywords:
Agricultural biotechnology Entomopathogens Liriomyza Science learning resources Science literacyReferences
Arfan, Anshary, A., Basri, Z., & Toana, H. (2018). Effect of Chemical Insecticides on the Arthropod Diversity in the Agroecosystem of Red Onion Crops. Asian Journal of Crop Science, 10(3), 107–114. https://doi.org/10.3923/ajcs.2018.107.114
Arfan, Basri, Z., Toana, H., Anshary, A., & Saleh, S. (2020). The Effectiveness of Abamectin Insecticide in Suppressing the Population of Liriomyza spp (Diptera: Agromysidae) on Red Onions. Indian Journal Of Agricultural Research, of. https://doi.org/10.18805/IJARe.A-473
Arsi, A., Pujiastuti, Y., Kusuma, S. S. H., & Gunawan, B. (2020). Eksplorasi, isolasi dan identifikasi Jamur entomopatogen yang menginfeksi serangga hama. Jurnal Proteksi Tanaman Tropis, 1(2), 70. https://doi.org/10.19184/jptt.v1i2.18554
Azhari, R., Nababan, R., & Hakim, L. (2021). Strategi Pengendalian Hama Tanaman Padi Dalam Peningkatan Produksi Pertanian Oleh Dinas Pertanian Kabupaten Karawang. JAS (Jurnal Agri Sains), 5(2), 199. https://doi.org/10.36355/jas.v5i2.785
Cahyaningtyas, R., Wilujeng, I., & Suryadarma, I. (2017). The Effect Of Science Learning Based On An Integrated Scientific Approach To Local Potential On The Science Process Skill Of The Student. Unnes Science Education Journal, 6. https://doi.org/10.15294/usej.v6i2.15857.
Chang, Y.-W., Chen, J.-Y., Zheng, S.-Z., Gao, Y., Chen, Y., Deng, Y., & Du, Y.-Z. (2020). Revalidation of morphological characteristics and multiplex PCR for the identification of three congener invasive Liriomyza species (Diptera: Agromyzidae) in China. PeerJ, 8, e10138. https://doi.org/10.7717/peerj.10138
da Silva, P. F., dos Santos, M. S. N., Araújo, B. de A., Kerber, B. D., de Oliveira, H. A. P., Guedes, J. V. C., Mazutti, M. A., Tres, M. V., & Zabot, G. L. (2025). Co-Cultivations of Beauveria bassiana, Metarhizium anisopliae, and Trichoderma harzianum to Produce Bioactive Compounds for Application in Agriculture. Fermentation, 11(1), 30. https://doi.org/10.3390/fermentation11010030
Fathy, Z., Muhammad, J., & Ali, S. S. (2025). The pathology of native entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Hypocreales: Clavicipitaceae), on biological processes and ovarian structure of the African migratory locust, Locusta migratoria migratorioides (Orthoptera: Acr. Egyptian Journal of Biological Pest Control, 35(1), 6. https://doi.org/10.1186/s41938-025-00842-7
Goettel, M. S., Eilenberg, J., & Glare, T. (2005). Entomopathogenic Fungi and their Role in Regulation of Insect Populations. In Comprehensive Molecular Insect Science (Vol. 6, pp. 361–405). Elsevier. https://doi.org/10.1016/B0-44-451924-6/00088-0
Hamid, Supartha, I. W., Susila, I. W., & Sudiarta, D. I. P. (2018). Morphological and molecular characteristics of liriomyza sp. (Diptera: Agromyzidae) on onion plants (Allium cepa L.) in Bali. Asian Journal of Agriculture and Biology, 6(4), 524–529. https://doi.org/10.35495/ajab.2018.07.217
Hastuti, P. W., Wilujeng, I., Susilowati, & Nurmadhani, T. (2022). Integration local potential “Ketingan Tourism Village” in science learning to improve critical thinking and scientific literacy based on the nature of science framework. AIP Conference Proceedings, 040001. https://doi.org/10.1063/5.0115347
Januarti, I. M., & Muliyadi, L. (2024). Development of Ethnoscience-Based Student Worksheet Using the Guided Inquiry Learning Model to Increase Students’ Learning Motivation and Scientific Literacy: A Review. International Journal of Science Education and Science, 1(1), 13–18. https://doi.org/10.56566/ijses.v1i1.109
Lapanjang, I., & Amirudin. (2023). Productivity of Lembah Palu Local Shallot (Allium cepa L. var. Aggregatum) from Organic Cultivation. Malaysian Applied Biology, 52(3), 49–58. https://doi.org/10.55230/mabjournal.v52i3.2634
McGuire, A. V., & Northfield, T. D. (2020). Tropical Occurrence and Agricultural Importance of Beauveria bassiana and Metarhizium anisopliae. Frontiers in Sustainable Food Systems, 4, 4. https://doi.org/10.3389/fsufs.2020.00006
Morris, J., Slater, E., Fitzgerald, M. T., Lummis, G. W., & van Etten, E. (2021). Using Local Rural Knowledge to Enhance STEM Learning for Gifted and Talented Students in Australia. Research in Science Education, 51(S1), 61–79. https://doi.org/10.1007/s11165-019-9823-2
Saif, I., Sufyan, M., Baboo, I., Jabbar, M., Shafiq, A., Saif, R. N., Liaqat, U., & Lackner, M. (2024). Efficacy of Beauveria bassiana and Metarhizium anisopliae against wheat aphid. The EuroBiotech Journal, 8(1), 23–31. https://doi.org/10.2478/ebtj-2024-0003
Samson, R. A., Evans, H. C., & Latgé, J.-P. (1988). Taxonomy of entomopathogenic fungi. In Atlas of Entomopathogenic Fungi (pp. 5–16). Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-662-05890-9_2
Sarah, M., Rendo, D., & Wahyuni, S. (2023). Penyuluhan Penerapan Teknologi Pengendalian Hama Siput Secara Terpadu. JMM (Jurnal Masyarakat Mandiri), 7(1), 915. https://doi.org/10.31764/jmm.v7i1.12833
Senthilkumar, M., Amaresan, N., & Sankaranarayanan, A. (2021). Galleria Bait Technique for the Isolation of Entomopathogenic Fungi from Soil. https://doi.org/10.1007/978-1-0716-1080-0_73
Supartha, I. W., Susila, I. W., Rauf, A., Shepard, B. M., Utama, I. W. E. K., Sandikayasa, I. W., Yudha, I. K. W., & Putu Angga Wiradana. (2023). Evaluation of the Community Structure Leafminer Fly, Liriomyza spp. (Diptera: Agromyzidae) and Their Parasitoids on Various Host Plant Families in Bali Province. HAYATI Journal of Biosciences, 30(3), 432–444. https://doi.org/10.4308/hjb.30.3.432-444
Susilawati, Doyan, A., Rokhmat, J., Muliyadi, L., Rizaldi, D. R., Fatimah, Z., Ikhsan, M., & Ardianti, N. R. (2025). Integration of Smartphone-Based Learning Media and Project-Based Learning to Enhance Creativity and Scientific Literacy in Physics. International Journal of Information and Education Technology, 15(7), 1449–1459. https://doi.org/10.18178/ijiet.2025.15.7.2346
Verawati, N. N. S. P., & Wahyudi, W. (2024). Raising the Issue of Local Wisdom in Science Learning and Its Impact on Increasing Students’ Scientific Literacy. International Journal of Ethnoscience and Technology in Education, 1(1), 42. https://doi.org/10.33394/ijete.v1i1.10881
Weintraub, P. G., Scheffer, S. J., Visser, D., Valladares, G., Soares Correa, A., Shepard, B. M., Rauf, A., Murphy, S. T., Mujica, N., MacVean, C., Kroschel, J., Kishinevsky, M., Joshi, R. C., Johansen, N. S., Hallett, R. H., Civelek, H. S., Chen, B., & Metzler, H. B. (2017). The Invasive Liriomyza huidobrensis (Diptera: Agromyzidae): Understanding Its Pest Status and Management Globally. Journal of Insect Science, 17(1). https://doi.org/10.1093/jisesa/iew121
Woo, S. L., Hermosa, R., Lorito, M., & Monte, E. (2023). Trichoderma: a multipurpose, plant-beneficial microorganism for eco-sustainable agriculture. Nature Reviews Microbiology, 21(5), 312–326. https://doi.org/10.1038/s41579-022-00819-5
Yuliandri, Adam, L., & Alnido, R. (2023). Evaluasi dan impact assessment pelatihan sekolah lapang pengendalian hama terpadu (SL-PHT) tanaman jagung melalui pengenalan dan aplikasi pestisida nabati di Kabupaten Purbalingga. Agrivet : Jurnal Ilmu-Ilmu Pertanian Dan Peternakan (Journal of Agricultural Sciences and Veteriner), 11(1), 84–89. https://doi.org/10.31949/agrivet.v11i1.6093
Zimmermann, G. (1986). The ‘Galleria bait method’ for detection of entomopathogenic fungi in soil. Journal of Applied Entomology, 102(1–5), 213–215. https://doi.org/10.1111/j.1439-0418.1986.tb00912.x
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