Experiment on the Effect of Pineapple Ecoenzyme on the Morphological Growth of Green Beans (Vigna radiata L.) as an Effort to Promote Environmentally Friendly Fertilization
DOI:
10.29303/jppipa.v11i12.13426Published:
2025-12-31Downloads
Abstract
This study aims to analyze the effectiveness of pineapple peel waste-based ecoenzymes as liquid organic fertilizer on the height growth of mung beans (Vigna radiata L.). Ecoenzymes are the result of organic waste fermentation with carbon sources from sugar and water, produced through the activity of decomposing microbes. The compounds contained therein, such as enzymes, organic acids, and beneficial microorganisms, play an important role in increasing soil biological activity, improving soil structure, and accelerating the absorption of nutrients by plant roots. The research was conducted in a laboratory experiment using a three-main-treatment design, namely: control without fertilizer, application of pineapple ecoenzyme liquid organic fertilizer, and application of EM4 liquid organic fertilizer as a commercial comparison. Observations were made every two days during a 14-day period, focusing on the variable of plant height increase. The results showed that the pineapple ecoenzyme treatment significantly increased vegetative plant growth, with an average growth of 10.40 cm, higher than the control (6.20 cm) and close to the EM4 results (11.10 cm). These findings confirm that pineapple ecoenzyme has the potential to be an environmentally friendly liquid organic fertilizer alternative, which not only supports plant growth but also contributes to sustainable household organic waste management
Keywords:
Ecoenzyme Green beans Liquid organic fertilizer Pineapple peel waste Vegetative growthReferences
Aguinis, H., & Solarino, A. M. (2019). Transparency and replicability in qualitative research: The case of interviews with elite informants. Strategic Management Journal, 40(8), 1291–1315. https://doi.org/10.1002/smj.3015 DOI: https://doi.org/10.1002/smj.3015
Aljarrah, M., Ashraf, A., Khandakar, A., Rohouma, W., Ayari, M. A., Esmaeili, A., Butt, R., Kadampotupadeth, S., Thomas, K., Rahman, A., & Phillips, M. (2024). Environmental performance analysis of three organic waste disposal scenarios: Landfilling, composting, and EP-50. Discover Sustainability, 5(1), 445. https://doi.org/10.1007/s43621-024-00614-7 DOI: https://doi.org/10.1007/s43621-024-00614-7
Amrullah, S., Amin, M., & Ali, M. (2021). Converting husbandry waste into liquid organic fertilizer using probiotic consortiums (Lactobacillus sp., Rhodopseudomonas sp., Actinomycetes sp., Streptomyces sp.). IOP Conference Series: Earth and Environmental Science, 679(1), 012001. https://doi.org/10.1088/1755-1315/679/1/012001 DOI: https://doi.org/10.1088/1755-1315/679/1/012001
Bhat, M. A., Mishra, A. K., Shah, S. N., Bhat, M. A., Jan, S., Rahman, S., Baek, K.-H., & Jan, A. T. (2024). Soil and Mineral Nutrients in Plant Health: A Prospective Study of Iron and Phosphorus in the Growth and Development of Plants. Current Issues in Molecular Biology, 46(6), 5194–5222. https://doi.org/10.3390/cimb46060312 DOI: https://doi.org/10.3390/cimb46060312
Chen, Q., Song, Y., An, Y., Lu, Y., & Zhong, G. (2024). Soil Microorganisms: Their Role in Enhancing Crop Nutrition and Health. Diversity, 16(12), 734. https://doi.org/10.3390/d16120734 DOI: https://doi.org/10.3390/d16120734
Das, D., Riamei, M., Paul, P., Singh, N., Ingti, B., Sarkar, R. D., Rose, R., Sharma, P. L., & Paul, S. (2025). Understanding the role of soil microorganisms in alleviating hydric and edaphic stress towards sustainable agriculture. Discover Soil, 2(1), 47. https://doi.org/10.1007/s44378-025-00076-x DOI: https://doi.org/10.1007/s44378-025-00076-x
De Bang, T. C., Husted, S., Laursen, K. H., Persson, D. P., & Schjoerring, J. K. (2021). The molecular–physiological functions of mineral macronutrients and their consequences for deficiency symptoms in plants. New Phytologist, 229(5), 2446–2469. https://doi.org/10.1111/nph.17074 DOI: https://doi.org/10.1111/nph.17074
Desmanto, D., Susilo, E., & Parwito, P. (2024). Pengaruh Pemberian Pupuk Organik Cair (POC) Berbahan Limbah Ikan Terhadap Pertumbuhan Dan Hasil Tanaman Sawi (Brassica juncea L.). SINTA Journal (Science, Technology, and Agricultural), 5(1), 115–124. https://doi.org/10.37638/sinta.5.1.115-124 DOI: https://doi.org/10.37638/sinta.5.1.115-124
Erika Erika & Eva Gusmira. (2024). Analisis Dampak Limbah Sampah Rumah Tangga Terhubung Pencemaran Lingkungan Hidup. Profit: Jurnal Manajemen, Bisnis Dan Akuntansi, 3(3), 90–102. https://doi.org/10.58192/profit.v3i3.2245 DOI: https://doi.org/10.58192/profit.v3i3.2245
Esercizio, N., Lanzilli, M., Vastano, M., Landi, S., Xu, Z., Gallo, C., Nuzzo, G., Manzo, E., Fontana, A., & d’Ippolito, G. (2021). Fermentation of Biodegradable Organic Waste by the Family Thermotogaceae. Resources, 10(4), 34. https://doi.org/10.3390/resources10040034 DOI: https://doi.org/10.3390/resources10040034
Gharge, V., Ghutake, S., & Pawar, H. (2024). Valorization of Pineapple Waste for Extraction and Purification of Bromelain Enzyme. ACS Sustainable Resource Management, 1(11), 2439–2451. https://doi.org/10.1021/acssusresmgt.4c00283 DOI: https://doi.org/10.1021/acssusresmgt.4c00283
Guggisberg, S. (2022). Transparency in the activities of the Food and Agriculture Organization for sustainable fisheries. Marine Policy, 136, 104498. https://doi.org/10.1016/j.marpol.2021.104498 DOI: https://doi.org/10.1016/j.marpol.2021.104498
Guo, H., Guan, M., Wang, Y., Li, X., Wang, H., He, C., & Chen, C. (2025). Research on the synergistic optimization of Scutellaria baicalensis yield, quality and soil environment by key microorganisms, driven by the combined approach of reducing chemical fertilizer usage while incorporating organic fertilizer. Industrial Crops and Products, 229, 121044. https://doi.org/10.1016/j.indcrop.2025.121044 DOI: https://doi.org/10.1016/j.indcrop.2025.121044
Hiywotu, A. M. (2025). Advancing sustainable agriculture for goal 2: Zero hunger - a comprehensive overview of practices, policies, and technologies. Agroecology and Sustainable Food Systems, 49(7), 1027–1055. https://doi.org/10.1080/21683565.2025.2451344 DOI: https://doi.org/10.1080/21683565.2025.2451344
Islam, M. R., Kamal, Mohd. M., Alam, M. A., Hossain, J., Soufan, W., Skalicky, M., Brestic, M., Habib-ur-Rahman, M., El Sabagh, A., & Islam, M. S. (2021). Physiochemical Changes of Mung Bean [Vigna radiata (L.) R. Wilczek] in Responses to Varying Irrigation Regimes. Horticulturae, 7(12), 565. https://doi.org/10.3390/horticulturae7120565 DOI: https://doi.org/10.3390/horticulturae7120565
Jia, Z., Giehl, R. F. H., & Von Wirén, N. (2022). Nutrient–hormone relations: Driving root plasticity in plants. Molecular Plant, 15(1), 86–103. https://doi.org/10.1016/j.molp.2021.12.004 DOI: https://doi.org/10.1016/j.molp.2021.12.004
Kitole, F. A., Ojo, T. O., Emenike, C. U., Khumalo, N. Z., Elhindi, K. M., & Kassem, H. S. (2024). The Impact of Poor Waste Management on Public Health Initiatives in Shanty Towns in Tanzania. Sustainability, 16(24), 10873. https://doi.org/10.3390/su162410873 DOI: https://doi.org/10.3390/su162410873
Kumar, S., Kumar, S., & Mohapatra, T. (2021). Interaction Between Macro‐ and Micro-Nutrients in Plants. Frontiers in Plant Science, 12, 665583. https://doi.org/10.3389/fpls.2021.665583 DOI: https://doi.org/10.3389/fpls.2021.665583
Lange, M., Eisenhauer, N., Sierra, C. A., Bessler, H., Engels, C., Griffiths, R. I., Mellado-Vázquez, P. G., Malik, A. A., Roy, J., Scheu, S., Steinbeiss, S., Thomson, B. C., Trumbore, S. E., & Gleixner, G. (2015). Plant diversity increases soil microbial activity and soil carbon storage. Nature Communications, 6(1), 6707. https://doi.org/10.1038/ncomms7707 DOI: https://doi.org/10.1038/ncomms7707
Li, J., Yi, C., Zhang, C., Pan, F., Xie, C., Zhou, W., & Zhou, C. (2021). Effects of light quality on leaf growth and photosynthetic fluorescence of Brasenia schreberi seedlings. Heliyon, 7(1), e06082. https://doi.org/10.1016/j.heliyon.2021.e06082 DOI: https://doi.org/10.1016/j.heliyon.2021.e06082
Maintang, Sudding, F., Asri, M., & Rauf, A. W. (2021). Application of liquid organic and inorganic fertilizer on growth and production of hybrid maize. IOP Conference Series: Earth and Environmental Science, 648(1), 012140. https://doi.org/10.1088/1755-1315/648/1/012140 DOI: https://doi.org/10.1088/1755-1315/648/1/012140
Martínez-Chávez, L. A., Hernández-Ramírez, M. Y., Feregrino-Pérez, A. A., & Esquivel Escalante, K. (2024). Cutting-Edge Strategies to Enhance Bioactive Compound Production in Plants: Potential Value of Integration of Elicitation, Metabolic Engineering, and Green Nanotechnology. Agronomy, 14(12), 2822. https://doi.org/10.3390/agronomy14122822 DOI: https://doi.org/10.3390/agronomy14122822
Marzouk, S. H., Kwaslema, D. R., Omar, M. M., & Mohamed, S. H. (2025). “Harnessing the power of soil microbes: Their dual impact in integrated nutrient management and mediating climate stress for sustainable rice crop production” A systematic review. Heliyon, 11(1), e41158. https://doi.org/10.1016/j.heliyon.2024.e41158 DOI: https://doi.org/10.1016/j.heliyon.2024.e41158
Mgeni, S. T., Mero, H. R., Mtashobya, L. A., & Emmanuel, J. K. (2024). The prospect of fruit wastes in bioethanol production: A review. Heliyon, 10(19), e38776. https://doi.org/10.1016/j.heliyon.2024.e38776 DOI: https://doi.org/10.1016/j.heliyon.2024.e38776
Mildaziene, V., Ivankov, A., Sera, B., & Baniulis, D. (2022). Biochemical and Physiological Plant Processes Affected by Seed Treatment with Non-Thermal Plasma. Plants, 11(7), 856. https://doi.org/10.3390/plants11070856 DOI: https://doi.org/10.3390/plants11070856
Muqsit, F. A., Setiadi, R., & Lo, A. (2024). Waste Management in Heritage Tourism Area: Perspectives from Visitors and Waste Management Operators. The Journal of Indonesia Sustainable Development Planning, 5(1), 15–26. https://doi.org/10.46456/jisdep.v5i1.524 DOI: https://doi.org/10.46456/jisdep.v5i1.524
Nanda, Lubis, R. A., Mukhlis, Nasution, F. E., & Sipahutar, L. W. (2025). Integrasi Evaluasi Eksperimental dan Analisis Bibliometrik Photosynthetic Bacteria dan Jakaba sebagai Bioaktivator Pertanian Organik. Jurnal Penelitian Pendidikan IPA, 11(7), 142–151. https://doi.org/10.29303/jppipa.v11i7.11789 DOI: https://doi.org/10.29303/jppipa.v11i7.11789
Paz, S. (2024). Climate change: A driver of increasing vector-borne disease transmission in non-endemic areas. PLOS Medicine, 21(4), e1004382. https://doi.org/10.1371/journal.pmed.1004382 DOI: https://doi.org/10.1371/journal.pmed.1004382
Pompelli, M. F., Jarma-Orozco, A., & Rodriguez-Páez, L. A. (2023). Imbibition and Germination of Seeds with Economic and Ecological Interest: Physical and Biochemical Factors Involved. Sustainability, 15(6), 5394. https://doi.org/10.3390/su15065394 DOI: https://doi.org/10.3390/su15065394
Rachman, I. A., Umasugi, B., Aji, K., Hakim, N. F. A., Sofyan, A., & Hasan, A. D. A. (2025). Effect of eco-enzyme application on soil nutrient and plant productivity of green mustard-peanut in inceptisol. Kultivasi, 24(2), 196–205. https://doi.org/10.24198/kultivasi.v24i2.64591 DOI: https://doi.org/10.24198/kultivasi.v24i2.64591
Sherzad, Z., Nawakht, N. A., & Sherzad, F. (2025). Plant growth-promoting endophytic bacteria: A sustainable solution for climate change and environmental stresses in agriculture. Discover Applied Sciences, 7(8), 894. https://doi.org/10.1007/s42452-025-07123-w DOI: https://doi.org/10.1007/s42452-025-07123-w
Suman, J., Rakshit, A., Ogireddy, S. D., Singh, S., Gupta, C., & Chandrakala, J. (2022). Microbiome as a Key Player in Sustainable Agriculture and Human Health. Frontiers in Soil Science, 2, 821589. https://doi.org/10.3389/fsoil.2022.821589 DOI: https://doi.org/10.3389/fsoil.2022.821589
Sun, Q., Zhang, Q., Huang, Z., Wei, C., Li, Y., & Xu, H. (2024). Effect of Organic Fertilizer Application on Microbial Community Regulation and Pollutant Accumulation in Typical Red Soil in South China. Agronomy, 14(9), 2150. https://doi.org/10.3390/agronomy14092150 DOI: https://doi.org/10.3390/agronomy14092150
Syllistiana, L. (2025). Process of Making Liquid Organic Fertilizer in Biotechnology Material. International Journal of Science Education and Science, 2(2), 74–77. https://doi.org/10.56566/ijses.v2i2.225 DOI: https://doi.org/10.56566/ijses.v2i2.225
Waheed, M., Mutahir Abbas, & Arif, S. H. (2023). Utilizing Internet of Thing for Construction Waste Management in Pakistan. Journal Of Digital Learning And Distance Education, 2(3), 506–512. https://doi.org/10.56778/jdlde.v2i3.134 DOI: https://doi.org/10.56778/jdlde.v2i3.134
Wawrzyniak, M. K., Michalak, M., & Chmielarz, P. (2020). Effect of different conditions of storage on seed viability and seedling growth of six European wild fruit woody plants. Annals of Forest Science, 77(2), 58. https://doi.org/10.1007/s13595-020-00963-z DOI: https://doi.org/10.1007/s13595-020-00963-z
Wei, X., Xie, B., Wan, C., Song, R., Zhong, W., Xin, S., & Song, K. (2024). Enhancing Soil Health and Plant Growth through Microbial Fertilizers: Mechanisms, Benefits, and Sustainable Agricultural Practices. Agronomy, 14(3), 609. https://doi.org/10.3390/agronomy14030609 DOI: https://doi.org/10.3390/agronomy14030609
Yan, J., Mackay, A. J., & Stone, C. M. (2024). Dynamics of invasive mosquitoes: Introduction pathways, limiting factors, and their potential role in vector-borne pathogen transmission. Frontiers in Tropical Diseases, 5, 1503120. https://doi.org/10.3389/fitd.2024.1503120 DOI: https://doi.org/10.3389/fitd.2024.1503120
Yoneyama, T. (2021). Iron delivery to the growing leaves associated with leaf chlorosis in mugineic acid family phytosiderophores-generating graminaceous crops. Soil Science and Plant Nutrition, 67(4), 415–426. https://doi.org/10.1080/00380768.2021.1947735 DOI: https://doi.org/10.1080/00380768.2021.1947735
Zhou, Z., Zhang, S., Jiang, N., Xiu, W., Zhao, J., & Yang, D. (2022). Effects of organic fertilizer incorporation practices on crops yield, soil quality, and soil fauna feeding activity in the wheat-maize rotation system. Frontiers in Environmental Science, 10, 1058071. https://doi.org/10.3389/fenvs.2022.1058071 DOI: https://doi.org/10.3389/fenvs.2022.1058071
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