Secretory Structure and Histochemistry of Leaf of Medicinal Plant Arcangelisia Flava
DOI:
10.29303/jppipa.v12i4.13773Published:
2026-05-04Downloads
Abstract
This study aimed to identify the tissue-level localization of major secondary metabolites and to describe the secretory structures of Arcangelisia flava leaves. Fresh transverse and paradermal hand sections of mature leaves were subjected to histochemical tests for flavonoids, phenolics, alkaloids, terpenoids, and lipophilic compounds. Flavonoid staining showed that flavones were the predominant class, accumulated strongly in the epidermis, while flavanones occurred only in guard cells; flavonols were not detected. Phenolic compounds were abundant in both the epidermis and mesophyll. Alkaloids also localized prominently in these tissues and around vascular bundles. Terpenoid detection using cupric acetate revealed intense deposits in epidermal and mesophyll idioblasts, whereas NADI staining indicated that essential oils were restricted to the epidermis. Lipophilic compounds were similarly concentrated in epidermal idioblasts. A consistent pattern was found in the epidermal cell rows flanking the leaf veins, which simultaneously accumulated flavonoids, phenolics, and terpenoids, suggesting a coordinated defensive zone along vascular pathways. The study also identified glandular hydathode trichomes containing flavones, phenolics, and lipophilic compounds, contrasting with previous descriptions of non-glandular trichomes in this species. This research provides the first detailed histochemical map of A. flava leaves and highlights key secretory features relevant to its medicinal value
Keywords:
Flavon Glandular Trichome Laticifer Secondary metabolites ParadermalReferences
Agati, G., Azzarello, E., Pollastri, S., & Tattini, M. (2012). Flavonoids as antioxidants in plants: location and functional significance. Plant Science, 196, 67–76. https://doi.org/10.1016/j.plantsci.2012.07.014 DOI: https://doi.org/10.1016/j.plantsci.2012.07.014
Ajayi, G. O., Kadiri, A. B., & Ibiloye, A. E. (2015). Pharmacognostic characteristics of the West African Jateorhiza macrantha (Hook. f.) Exell & Mendonça (Menispermaceae. International Journal of Pharmacognosy and Phytochemical Research, 7(5), 1001–1006. Retrieved from https://shorturl.asia/r9uVz
Boix, Y. F., Victório, C. P., Defaveri, A. C. A., Arruda, R. D. D. O., & Sato, A. (2011). Glandular trichomes of Rosmarinus officinalis L.: Anatomical and phytochemical analyses of leaf volatiles. Plant Biosystems, 145(4), 848–856. https://doi.org/10.1080/11263504.2011.584075 DOI: https://doi.org/10.1080/11263504.2011.584075
Bourdon, M., Gaynord, J., Müller, K. H., Evans, G., Wallis, S., Aston, P., Spring, D. R., & Wightman, R. (2021). Microscopy and chemical analyses reveal flavone-based woolly fibres extrude from micron-sized holes in glandular trichomes of Dionysia tapetodes. BMC Plant Biology, 21(1), 258. https://doi.org/10.1186/s12870-021-03010-9 DOI: https://doi.org/10.1186/s12870-021-03010-9
Cheng, Q., Li, F., Yan, X., He, J., Zhang, H., Wang, C., & Li, Z. (2021). Phytochemical and pharmacological studies on the genus Arcangelisia: A mini review. Arabian Journal of Chemistry, 14(10), 103346. https://doi.org/10.1016/j.arabjc.2021.103346 DOI: https://doi.org/10.1016/j.arabjc.2021.103346
Demarco, D. (2017). Histochemical analysis of plant secretory structures. Methods in Molecular Biology, 1560, 313–330. https://doi.org/10.1007/978-1-4939-6788-9_24 DOI: https://doi.org/10.1007/978-1-4939-6788-9_24
Diliarosta, S., Sudarmin, E., A., D., D., O., B., & Ramadhani, R. (2021). Reconstruction and scientific explanation of Akar Kuning (Arcangelisia flava Merr.) from West Sumatra as ethnomedicine and source of science learning. Pharmacognosy Journal, 13(1), 206–211. Retrieved from https://shorturl.asia/Cswn6 DOI: https://doi.org/10.5530/pj.2021.13.29
Falcone Ferreyra, M. L., Serra, P., & Casati, P. (2021). Recent advances on the roles of flavonoids as plant protective molecules after UV and high light exposure. Physiologia Plantarum, 173(3), 736–749. https://doi.org/10.1111/ppl.13463 DOI: https://doi.org/10.1111/ppl.13543
Forman, L. L. (1984). Menispermaceae. Flora Malesiana - Ser 1, Spermatophyta. Flora Malesiana-Series 1, Spermatophyta, 10(1), 157–253. Retrieved from https://repository.naturalis.nl/pub/532690
Franceschi, V. R., & Nakata, P. A. (2005). Calcium oxalate in plants: Formation and function. Annual Review of Plant Biology, 56, 41–71. https://doi.org/10.1146/annurev.arplant.56.032604.144106 DOI: https://doi.org/10.1146/annurev.arplant.56.032604.144106
Guedes, J. G., Ribeiro, R., Carqueijeiro, I., Guimarães, A. L., Bispo, C., Archer, J., Azevedo, H., Fonseca, N. A., & Sottomayor, M. (2023). The leaf idioblastome of the medicinal plant Catharanthus roseus is associated with stress resistance and alkaloid metabolism. Journal of Experimental Botany, 75(1), 274–299. https://doi.org/10.1093/jxb/erad374 DOI: https://doi.org/10.1093/jxb/erad374
Guerin, H. P., Delaveau, P. G., & Paris, R. R. (1971). Localisations histochimiques. II: Procédés simples de localisation de pigments flavoniques: Application à quelques phanérogames. Bulletin de La Société Botanique de France, 118(1–2), 29–36. https://doi.org/10.1080/00378941.1971.10838874 DOI: https://doi.org/10.1080/00378941.1971.10838874
Hendra, R., Agustha, A., Frimayanti, N., Abdulah, R., & Teruna, H. Y. (2024). Antifungal potential of secondary metabolites derived from Arcangelisia flava (L.) Merr.: An analysis of in silico enzymatic inhibition and in vitro efficacy against Candida species. Molecules, 29(10), 2373. https://doi.org/10.3390/molecules29102373 DOI: https://doi.org/10.3390/molecules29102373
Heryani, H., & Nugroho, A. (2015). Study of yellow root (Arcangelisia flava Merr) as a natural food additive with antimicrobial and acidity-stabilizing effects in the production process of palm sugar. Procedia Environmental Sciences, 23, 346–350. https://doi.org/10.1016/j.proenv.2015.01.050 DOI: https://doi.org/10.1016/j.proenv.2015.01.050
Hilmi, M., Hamim, H., Sulistyaningsih, Y. C., & Taufikurahman. (2018). Growth, histochemical and physiological responses of non-edible oil producing plant (Reutealis trisperma) to gold mine tailings. Biodiversitas, 19(4), 1294–1302. https://doi.org/10.13057/biodiv/d190416 DOI: https://doi.org/10.13057/biodiv/d190416
Kabbashi, A. S., Sattar, M. A., Aamer, M., Siddiqui, N. N., Kamran, M., Fayaz, A., Jahan, H., Khan, F.-A., & Wang, Y. (2024). Clerodane furanoditerpenoids from Tinospora bakis (A. Rich.) Miers (Menispermaceae. Molecules, 29(1), 154. https://doi.org/10.3390/molecules29010154 DOI: https://doi.org/10.3390/molecules29010154
Kaczmarek, B. (2020). Tannic Acid with Antiviral and Antibacterial Activity as A Promising Component of Biomaterials—A Minireview. Materials, 13(14), 3224. https://doi.org/10.3390/ma13143224 DOI: https://doi.org/10.3390/ma13143224
Kamilah, S. N., Andriyani, E. F. S., Putri, D. A., Astuti, S., Manaf, S., & Saputra, H. M. (2022). Efektivitas ekstrak batang Arcangelisia flava Merr. sebagai afrodisiaka pada mencit jantan dan betina. BIOEDUSAINS, 5(1), 85–93. https://doi.org/10.31539/bioedusains.v5i1.2426 DOI: https://doi.org/10.31539/bioedusains.v5i1.2426
Kromer, K., Kreitschitz, A., Kleinteich, T., Gorb, S. N., & Szumny, A. (2016). Oil secretory system in vegetative organs of three Arnica taxa: essential oil synthesis, distribution and accumulation. Plant and Cell Physiology, 57(5), 1020–1037. https://doi.org/10.1093/pcp/pcw040 DOI: https://doi.org/10.1093/pcp/pcw040
Long, J., Song, J., Zhong, L., Liao, Y., Liu, L., & Li, X. (2019). Palmatine: A review of its pharmacology, toxicity and pharmacokinetics. Biochimie, 162, 176–184. https://doi.org/10.1016/j.biochi.2019.04.008 DOI: https://doi.org/10.1016/j.biochi.2019.04.008
Lv, C., Li, Y., Liang, R., Huang, W., Xiao, Y., Ma, X., Wang, Y., Zou, H., Qin, F., Sun, C., Li, T., & Zhang, J. (2023). Characterization of tangeretin as an activator of nuclear factor erythroid 2-related factor 2/antioxidant response element pathway in HEK293T cells. Current Research in Food Science, 6, 100459. https://doi.org/10.1016/j.crfs.2022.100459 DOI: https://doi.org/10.1016/j.crfs.2023.100459
Mandia, E. H., Ridsdale, C. E., Horsten, S. F. A. J., & Aguinaldo, A. M. (1999). Arcangelisia flava (L.) Merr Plant Resources of South-East Asia. In L. S. Padua, N. Bunyapraphatsara, & R. H. M. H. Lemmens (Eds.), Medicinal and Poisonous Plants 1. Backhuys Publishers. Retrieved from https://shorturl.asia/mGY4Z
McLachlan, D. H., Lan, J., Geilfus, C. M., Dodd, A. N., Larson, T., Baker, A., Hõrak, H., Kollist, H., He, Z., Graham, I., Mickelbart, M. V, & Hetherington, A. M. (2016). The Breakdown of Stored Triacylglycerols Is Required during Light-Induced Stomatal Opening. Current Biology : CB, 26(5), 707–712. https://doi.org/10.1016/j.cub.2016.01.019 DOI: https://doi.org/10.1016/j.cub.2016.01.019
Meenu, M. T., & Radhakrishnan, K. V. (2020). Menispermaceae family of plants and its action against infectious diseases: A review. Mapana Journal of Sciences, 19(2), 33–71. https://doi.org/10.12723/mjs.53.4 DOI: https://doi.org/10.12723/mjs.53.4
Mutiah, R., Kirana, F. O., Annisa, R., Rahmawati, A., & Sandra, F. (2020). Extract of yellow root (Arcangelisia flava (L.) Merr.) from several regions in kalimantan: alkaloid content and cytotoxicity towards widr colorectal cancer cells. Indonesian Journal of Cancer Chemoprevention, 11(2), 84–89. 10.14499/indonesianjcanchemoprev11iss2pp84-89 DOI: https://doi.org/10.14499/indonesianjcanchemoprev11iss2pp84-89
Pratama, R. R., Sholikhah, I., Sahu, R. K., & Widyowati, R. (2023). Phytochemical compounds identification from 70% ethanol extract of Arcangelesia flava (L.) Merr stems using LC-MS/MS and in-silico molecular docking approach as inhibitor interleukin-1β. Pharmacognosy Journal, 15(4), 528–534. https://doi.org/10.5530/pj.2023.15.114 DOI: https://doi.org/10.5530/pj.2023.15.114
Ribeiro, M., Amaral, F. M., Anholeti, M. C., Junior, E. C. B., Valverde, A. L., Tamaio, N., & Alvarenga, J. M. (2022). Chemical Profile, Antioxidant Activity, Flavonoids Content and Leaf Anatomy of Odontocarya vitis (Menispermaceae). Biointerface Research in Applied Chemistry, 13(2). https://doi.org/10.33263/BRIAC132.128 DOI: https://doi.org/10.33263/BRIAC132.128
Rupa, D., Sulistyaningsih, Y., & Ratnadewi, D. D. (2017). Identification of secretory structure, histochemistry and phytochemical compounds of medicinal plant Hyptis capitata Jacq. BIOTROPIA, 24(2), 94–103. https://doi.org/10.11598/btb.2017.24.2.499 DOI: https://doi.org/10.11598/btb.2017.24.2.499
Santoso, T. I., Miftahudin, M., Sulistyaningsih, Y. C., & Wiyono, S. (2017). Analysis of Secondary Metabolites as Potential Phytoalexins, Their Secretion Sites and Proposed Resistance Markers to Vascular Streak Dieback in Theobroma cacao L. Pelita Perkebunan, 33(1), 10–23. Retrieved from https://www.ccrjournal.com/index.php/ccrj/article/view/250 DOI: https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v33i1.250
Tavita, G. E., Lestari, D., Linda, R., Apindiati, R. K., & Rafdinal, R. (2022). Phytochemical testing and in vitro anti-inflammatory activity on ethanol extract of akar kuning (Arcangelisia flava L) stems from West Kalimantan. Jurnal Biologi Tropis, 22(4), 1334–1339. https://doi.org/10.29303/jbt.v22i4.4431 DOI: https://doi.org/10.29303/jbt.v22i4.4431
Wahyudi, L. D., Ratnadewi, A. A. I., & Siswoyo, T. A. (2016). Potential antioxidant and antidiabetic activities of kayu kuning (Arcangelisia flava. Agriculture and Agricultural Science Procedia, 9, 396–402. https://doi.org/10.1016/j.aaspro.2016.02.155 DOI: https://doi.org/10.1016/j.aaspro.2016.02.155
Widuri, S. A., Sulistyaningsih, Y. C., Umar, A. H., & Ratnadewi, D. (2024). Secretory structures and histochemistry of roots, stem, and leaves of medicinal plant Fibraurea tinctoria Lour. Microscopy Research and Technique, 87(12), 3026–3036. https://doi.org/10.1002/jemt.24676 DOI: https://doi.org/10.1002/jemt.24676
Wilkinson, H. P. (1978). Leaf anatomy of the tribe Coscinieae Hook. f. & Thoms. Menispermaceae). Kew Bulletin, 32(2), 347–360. Retrieved from https://www.jstor.org/stable/4117107 DOI: https://doi.org/10.2307/4117107
Xi, K.-Y., Xiong, S.-J., Li, G., Guo, C.-Q., Zhou, J., Ma, J.-W., Yin, J.-L., Liu, Y.-Q., & Zhu, Y.-X. (2022). Antifungal activity of ginger rhizome extract against Fusarium solani. Horticulturae, 8(11), 983. https://doi.org/10.3390/horticulturae8110983 DOI: https://doi.org/10.3390/horticulturae8110983
Xie, Y., Yang, W., Tang, F., Chen, X., & Ren, L. (2015). Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Current Medicinal Chemistry, 22(1), 132–149. https://doi.org/10.2174/0929867321666140916113443 DOI: https://doi.org/10.2174/0929867321666140916113443
Yang, J., Chen, Y. Z., Yu-Xuan, W., Tao, L., Zhang, Y. D., Wang, S. R., Zhang, G. C., & Zhang, J. (2021). Inhibitory effects and mechanisms of vanillin on gray mold and black rot of cherry tomatoes. Pesticide Biochemistry and Physiology, 175, 104859. https://doi.org/10.1016/j.pestbp.2021.104859 DOI: https://doi.org/10.1016/j.pestbp.2021.104859
Zhang, Y., Wang, D., Li, H., Bai, H., Sun, M., & Shi, L. (2023). Formation mechanism of glandular trichomes involved in the synthesis and storage of terpenoids in lavender. BMC Plant Biology, 23(1), 307. https://doi.org/10.1186/s12870-023-04275-y DOI: https://doi.org/10.1186/s12870-023-04275-y
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