Potential Effectiveness of Goji Berry (Lycium barbarum L.) Extract on Neutrophil Cell Count in Wistar Rats (Rattus norvegicus) Post Tooth Extraction
DOI:
10.29303/jppipa.v12i1.13937Published:
2026-01-25Downloads
Abstract
Tooth extraction causes tissue injury that triggers an inflammatory response, which is characterized by an increase in neutrophil counts. Although neutrophils play an essential role in the early phase of wound healing, prolonged inflammation may delay tissue regeneration. Goji berry (Lycium barbarum L.) contains various bioactive compounds that have potential anti-inflammatory effects. This study aimed to evaluate the effectiveness of goji berry extract on neutrophil counts in Wistar rats (Rattus norvegicus) following tooth extraction. This study was experimental laboratory research using a post-test group design. A total of 30 male Wistar rats were divided into five groups: negative control, positive control (povidone iodine), and three treatment groups receiving goji berry extract at concentrations of 15%, 30%, and 55%. The extract was formulated as a gel and applied topically to the extraction socket. Neutrophil counts were evaluated on days 1, 3, 5, and 7 through histopathological examination. Data were analyzed using the Shapiro–Wilk test, One Way ANOVA, and the LSD post hoc test. The results showed significant differences in neutrophil counts among groups on days 3, 5, and 7 (p<0.05). The 55% concentration demonstrated the most optimal reduction in neutrophil counts. It can be concluded that goji berry extract has potential as an alternative herbal therapy to support wound healing after tooth extraction.
Keywords:
Goji berry Lycium barbarum L. Neutrophils Tooth extraction Wound healingReferences
Anggayanti, N. A., Dewi, I. A. N. N., & Dewi, D. A. D. N. (2024). Efektivitas Gel Ekstrak Ocimum basilicum L . 15 % terhadap jumlah sel neutrofil pada fase inflamasi pasca Ekstraksi gigi tikus wistar : Studi neutrophil cell count in the inflammatory phase after tooth. Jurnal Kedokteran Gigi Universitas Padjadjaran, 36(3), 371–379. https://doi.org/10.24198/jkg.v36i3.58977 DOI: https://doi.org/10.24198/jkg.v36i3.58977
Ávila, C. N., Trindade, F. M. R., Penteado, J. O., & Janke, F. (2020). Anti-inflammatory Effect of a Goji Berry Extract (Lycium barbarum) in Rats Subjected to Inflammation by Lipopolysaccharides (LPS). Brazilian Archives of Biology and Technology, 63, 1–11. https://dx.doi.org/10.1590/1678-4324-2020180612 DOI: https://doi.org/10.1590/1678-4324-2020180612
Azizah, K. U., Anwaristi, A. Y., Kurniawati, D., & Ningsih, J. R. (2024). Efektivitas Gel Ekstrak Daun Binahong (Anredera cordifolia) 5% Terhadap Jumlah Neutrofil pada Proses Penyembuhan Luka Pasca Pencabutan Gigi Tikus strain Wistar. ARTERI: Jurnal Ilmu Kesehatan, 5(3), 1–8. https://doi.org/10.37148/arteri.v5i3.434 DOI: https://doi.org/10.37148/arteri.v5i3.434
Barboura, M., Cornebise, C., Hermetet, F., Guerrache, A., Selmi, M., Salek, A., Chekir-ghedira, L., Aires, V., & Delmas, D. (2022). Tannic Acid, A Hydrolysable Tannin, Prevents Transforming Growth Factor-β-Induced Epithelial–Mesenchymal Transition to Counteract Colorectal Tumor Growth. Cells, 11(3645), 1–19. https://doi.org/10.3390/cells11223645 DOI: https://doi.org/10.3390/cells11223645
Borges, A., Vaz, J. A., & Calhelha, R. C. (2024). Enhancing Wound Healing : A Comprehensive Review of Sericin and Chelidonium majus L . as Potential Dressings. Materials, 17(4199), 1–43. https://doi.org/10.3390/ma17174199 DOI: https://doi.org/10.3390/ma17174199
Cao, L., Ma, L., Zhao, J., Wang, X., Fang, X., & Li, W. (2023). An Unexpected Role of Neutrophils in Clearing Apoptotic Hepatocytes in Vivo. ELife, 20(12), 1–26. https://doi.org/10.7554/eLife.86591 DOI: https://doi.org/10.7554/eLife.86591
Cui, C., Zhao, D., Huang, J., & Hao, J. (2022). Progress on research and development of goji berry drying: A review. International Journal of Food Properties, 25(1), 435–449. https://doi.org/10.1080/10942912.2022.2046054 DOI: https://doi.org/10.1080/10942912.2022.2046054
Djuma, E., & Kurnia, D. A. (2024). Efektivitas penggunaan terapi topical herbal pada perawatan ulkus kaki diabetik: A systematic literature review. Holistik Jurnal Kesehatan, 18(2), 202–208. https://doi.org/10.33024/hjk.v18i2.281 DOI: https://doi.org/10.33024/hjk.v18i2.281
Emilia, I., Setiawan, A. A., Novianti, D., Rangga, & Mutiara, D. (2023). Skrining Fitokimia Ekstrak Daun Sugkai (Peronema canescens Jack.) secara Infundasi dan Maserasi. Jurnal Indobiosains, 5(2), 95–102. https://doi.org/10.31851/indobiosains.v5i2.12597 DOI: https://doi.org/10.31851/indobiosains.v5i2.12597
Gao, M., Guo, H., Dong, X., Wang, Z., & Yang, Z. (2024). Regulation of Inflammation During Wound Healing : the Function of Mesenchymal Stem Cells and Strategies for Therapeutic Enhancement. Frontiers in Pharmacology, 15(February), 1–15. https://doi.org/10.3389/fphar.2024.1345779 DOI: https://doi.org/10.3389/fphar.2024.1345779
Halim, S., Halim, H., Lister, I. N. E., Sihotang, S., Nasution, A. N., & Girsang, E. (2021). Efektivitas gel ekstrak etanol daun senggani (Melastoma candidum D. Don.) terhadap diameter luka pasca pencabutan gigi pada tikus putih (Rattus norvegicus). Bioma: Jurnal Ilmiah Biologi, 10(1), 44–54. https://doi.org/10.26877/bioma.v10i1.6555 DOI: https://doi.org/10.26877/bioma.v10i1.6555
Harun, H., Haroen, H., Mirwanti, R., Apriani, N., & Akuoko, C. P. (2024). Uncovering the Benefits of Povidone Iodine Compared to Other Therapeutic Agents in Wound Infection Prevention and Healing Outcomes : A Scoping Review. Journal of Multidisciplinary Healthcare, 17(July), 3605–3616. https://doi.org/10.2147/JMDH.S469037 DOI: https://doi.org/10.2147/JMDH.S469037
Hervina, H., Haryani, I. G. A. D., & Aryandana, N. M. D. P. (2024). Pemberian Vitamin D Menyebabkan Jumlah Sel Neutrofil Lebih Rendah Dibanding Kontrol pada Tikus Wistar yang Diinduksii Periodontitis. Bali Dental Science and Exhibition (Bali Dence), 28, 260–272. Retrieved from https://e-journal.unmas.ac.id/index.php/BDSE/article/view/10747
Istiqomah, I. N., Mashuri, Abdillah, A., & Azizah, L. N. (2024). Potensi Tanaman Ganjan (Artemisia vulgaris) dalam Penyembuhan Luka Sayat pada Tikus (Sprague Dawley). Jurnal Tumbuhan Obat Indonesia, 17(1), 56–65. https://doi.org/10.31002/jtoi.v16i2.885 DOI: https://doi.org/10.31002/jtoi.v17i1.1159
Jomova, K., Alomar, S. Y., Valko, R., Liska, J., Nepovimova, E., Kuca, K., Valko, M., & Valko, M. (2025). Chemico-Biological Interactions Flavonoids and their Role in Oxidative Stress, Inflammation, and Human Diseases. Chemico-Biological Interactions, 413(February), 1–44. https://doi.org/10.1016/j.cbi.2025.111489 DOI: https://doi.org/10.1016/j.cbi.2025.111489
Margraf, A., Lowell, C. A., & Zarbock, A. (2022). Neutrophils in Acute Inflammation : Current Concepts and Translational Implications. Blood, 139(14), 2130–2144. https://doi.org/10.1182/blood.2021012295 DOI: https://doi.org/10.1182/blood.2021012295
Oki, A. S., & Amalia, N. (2020). Wound healing acceleration in inflammation phase of post-tooth extraction after aerobic and anaerobic exercise. Science & Sports, 35(3), 168-e1. https://doi.org/10.1016/j.scispo.2019.06.001 DOI: https://doi.org/10.1016/j.scispo.2019.06.001
Prabhath, S., Bhat, K. M. R., Pai, K. S. R., Kinra, M., & Gourishetti, K. (2024). A Biochemical and Molecular Insight to Wound Healing Properties of Traditional Indian Medicines in Normal and Diabetic Rats. Ind. J. Pharm. Edu. Res, 58(2), 359–371. https://doi.org/10.5530/ijper.58.2s.39 DOI: https://doi.org/10.5530/ijper.58.2s.39
Prehananto, H., Purnamasari, V. D., & Yuliastri, N. (2023). Uji Aktivitas Ekstrak Daun Kemangi dalam Meningkatkan Jumlah Fibroblas pada Traumatik Ulser. Bhakta Dental Jurnal, 1(1), 1–8. Retrieved from https://bdj.iik.ac.id/index.php/bdj/article/view/11
Prestiyanti, N. M. I., Adiatmika, I. P. G., & Muliarta, I. M. (2021). Pemberian pasta ekstrak daun pegagan 10% lebih meningkatkan jumlah sel fibroblas dan reepitelisasi pada soket mandibula daripada pasta ekstrak daun mengkudu 10% pasca pencabutan gigi marmut jantan. Intisari Sains Medis, 12(3), 718–723. https://doi.org/10.15562/ism.v12i3.1114 DOI: https://doi.org/10.15562/ism.v12i3.1114
Purnama, H., Sriwidodo, & Ratnawulan, S. (2017). Review Sistematik: Proses Penyembuhan Dan Perawatan Luka. Farmaka, 15(2), 251–258. https://doi.org/10.24198/jf.v15i2.13366
Putri, E. B. P., Putri, F. K., & Sulaiha, S. (2020). Perbandingan Kadar Flavonoid dan Vitamin C pada Infused Water Goji Berry (Lycium barbarum) dan Air Nabeez Kurma (Phoenix dactylifera L.). MTPH, 4(1), 32–37. https://doi.org/10.33086/mtphj.v4i1.1458 DOI: https://doi.org/10.33086/mtphj.v4i1.1458
Rahmawati, A. A., Harijadi, A., Kristanto, Y., & Asmarani, Y. K. (2023). Pengaruh Gel Ekstrak Bawang Merah terhadap Jumlah Fibroblas pada Luka Ekstraksi Gigi Tikus Jantan Wistar. Bhakta Dental Journal, 1(2), 1–7. https://doi.org/10.56710/bhaktadent.v1i2.17 DOI: https://doi.org/10.56710/bhaktadent.v1i2.17
Riyani, N. J., Pasaribu, R., Mardiyantoro, K. F., & Gigi, F. K. (2021). Evaluasi Jumlah Limfosit Pasca Aplikasi Lendir Bekicot (Achatina fulica) pada Soket Tikus Wistar (Rattus norvegicus). Sinnun Maxillofacial Journal, 3(1), 42–49. https://doi.org/10.33096/smj.v3i01.31 DOI: https://doi.org/10.33096/smj.v3i01.31
Skenderidis, P., Leontopoulos, S., & Lampakis, D. (2022). Goji Berry : Health Promoting Properties. Nutraceuticals, 2(1), 32–48. https://doi.org/10.3390/nutraceuticals2010003 DOI: https://doi.org/10.3390/nutraceuticals2010003
Uribe-querol, E., & Rosales, C. (2024). Neutrophils versus Protozoan Parasites : Plasmodium ,. Microorganisms, 12(827), 1–32. https://doi.org/10.3390/microorganisms12040827 DOI: https://doi.org/10.3390/microorganisms12040827
Yang, L., Shi, F., & Wang, L. (2025). Neutrophils in Tissue Injury and Repair : Molecular Mechanisms and Therapeutic Targets. MedComm, 6(e70184), 1–18. https://doi.org/10.1002/mco2.70184 DOI: https://doi.org/10.1002/mco2.70184
Ysrafil, Y., Sapiun, Z., Slamet, N. S., Mohamad, F., Hartati, H., Damiti, S. A., Alexandra, F. D., Rahman, S., Masyeni, S., Harapan, H., & Mamada, S. S. (2023). Anti-inflammatory Activities of Flavonoid Derivates. ADMET & DMPK, 11(3), 331–359. https://doi.org/10.5599/admet.1918 DOI: https://doi.org/10.5599/admet.1918
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