Development of an Antibacterial Poster Based on Papaya Leaf Starch (Carica papaya L.) Against Escherichia coli for the Community
DOI:
10.29303/jppipa.v12i1.14027Published:
2026-01-25Downloads
Abstract
In recent years, the use of natural ingredients as antibacterial agents has gained increasing attention due to the increasing antibiotic resistance. One such potential is papaya leaf extract (Carica papaya L.), which has shown antibacterial activity against Escherichia coli, a common cause of diarrhea in the community. This study aims to develop an educational poster that communicates the antibacterial benefits of papaya leaf extract against Escherichia coli. This study used the Research and Development (R&D) model as modified by Borg & Gall, which consists of five stages: (1) initial information collection, (2) planning, (3) initial product development, (4) limited field testing, and (5) product revision. Validation was carried out by material, media, and language experts, while readability testing was conducted on individuals aged ≥17 years. Data analysis included qualitative content analysis and quantitative descriptive analysis to evaluate the feasibility and readability of the poster. The results showed that the developed poster obtained a validation score above 80% from all experts, and a highest readability score of 95% from the community, indicating that the poster was very feasible, informative, and easy to understand. The strength of the poster lies in its attractive visual design, communicative language, and concise and focused content. This educational media is expected to increase public awareness of diarrhea prevention using herbal plants. Further research is recommended to explore the long-term impact of the media on behavioral change and assess the antibacterial effectiveness of papaya leaf extract in various formulations.
Keywords:
Antibacterial Educational media Escherichia coli Papaya leaves PosterReferences
Adnan, D. R. A., Kaloko, J., Sihombing, K., & Mahmud, P. (2022). Pengembangan Media Poster sebagai Media Edukasi Orangtua dalam Membantu Mencegah Perilaku Perundungan. JKKP (Jurnal Kesejahteraan Keluarga dan Pendidikan), 9(02), 234–242. https://doi.org/10.21009/JKKP.092.10 DOI: https://doi.org/10.21009/JKKP.092.10
Ahman, H. A., Muhammad, U. K., Fardami, A. Y., Muhammad, M. Z., & Aliyu, R. M. (2024). Prevalence of Antibiotic Resistant Diarrheagenic E. Coli Isolated from Stool Samples of Diarrheic Children Under 5 Years in Sokoto, Nigeria. UMYU Journal of Microbiology Research (UJMR), 489–498. https://doi.org/10.47430/ujmr.2493.056 DOI: https://doi.org/10.47430/ujmr.2493.056
Bogoevska-Gavrilova, I., & Ciunova-Shuleska, A. (2024). Decoding Source Credibility: How it Influences Trust in Informative Brand-Related Content and Customer Purchase Intentions. In Proceedings of the 5th International Conference" Economic and Business Trends Shaping the Future" 2024 (No. 002, pp. 18-22). https://doi.org/10.47063/EBTSF.2024.0002 DOI: https://doi.org/10.47063/EBTSF.2024.0002
Burdujan, R. (2024). Decoding Didactic Discourse Strategies in Digital Posters. InterConf, 50(221), 71–79. https://doi.org/10.51582/interconf.19-20.10.2024.009 DOI: https://doi.org/10.51582/interconf.19-20.10.2024.009
Burnett, R. E., & Hoffman, C. A. (2025). A Conceptual Approach to Research Poster Design: Process, Audience, and Organization. Technical Communication Quarterly, 1–21. https://doi.org/10.1080/10572252.2024.2445295 DOI: https://doi.org/10.1080/10572252.2024.2445295
Choudhary, R., Kaushik, R., Akhtar, A., Manna, S., Sharma, J., & Bains, A. (2025). Nutritional, Phytochemical, and Antimicrobial Properties of Carica Papaya Leaves: Implications for Health Benefits and Food Applications. Foods, 14(2), 154. https://doi.org/10.3390/foods14020154 DOI: https://doi.org/10.3390/foods14020154
Davies, N. A., Deutz, N. E., Delzenne, N., & Lobo, D. N. (2024). Presentation and Publication Skills: How to Prepare a Scientific Poster. Clinical Nutrition ESPEN, 63, 457–461. https://doi.org/10.1016/j.clnesp.2024.06.056 DOI: https://doi.org/10.1016/j.clnesp.2024.06.056
De Lacerda, D. A., Lima, L. L. L., Honorato, P. F., Vale, R. B., Das Neves, D. F., De Sá Filho, C. P., De Moura, T. Q., Figueiredo, V. C., De Oliveira, A. V. L., & Isidório, U. de A. (2024). Pharmacology and Pharmacotherapy of Antimicrobials. Revista Sistemática, 14(2), 306–310. https://doi.org/10.56238/rcsv14n2-024 DOI: https://doi.org/10.56238/rcsv14n2-024
Feletto, L. (2024). Sci-Comm a Long Way: My Journey from Research to Teaching (and beyond?). The Biochemist, 46(4), 19–22. https://doi.org/10.1042/bio_2024_140 DOI: https://doi.org/10.1042/bio_2024_140
Habibi, A. R., Wullur, W. A. I., Palapessy, V. E. D., & Maulana, R. A. (2024). Uji Aktivitas Tangkai Daun Pepaya sebagai Antibakteri Escherichia Coli. Jurnal Ilmiah Kesehatan Masyarakat dan Sosial, 2(4), 26–32. https://doi.org/10.59024/jikas.v2i4.961 DOI: https://doi.org/10.59024/jikas.v2i4.961
Hanindyo, M. H., & Rachman, B. E. (2024). The Role of Antibiotics in the Treatment of Bacterial Infections: A Literature Review. Magna Scientia Advanced Research and Reviews, 12(2), 410–413. https://doi.org/10.30574/msarr.2024.12.2.0226 DOI: https://doi.org/10.30574/msarr.2024.12.2.0226
Ingolo, H. A., & Ong’onda, A. N. (2024). Health Communication: A Multimodal Discourse Analysis of Selected Corona Virus Posters in Kenya. African Journal of Empirical Research, 5(4), 1531–1545. https://doi.org/10.51867/ajernet.5.4.128 DOI: https://doi.org/10.51867/ajernet.5.4.128
Joy, A.-O. (2023). Multimodal Analysis of Selected Health Awareness Materials: A Visual Grammar Approach. South Asian Research Journal of Arts, Language and Literature, 5(04), 97–107. https://doi.org/10.36346/sarjall.2023.v05i04.002 DOI: https://doi.org/10.36346/sarjall.2023.v05i04.002
Kılıç, N., & Gültekin, G. (2024). Sustainable Approaches in Aquaculture: Pharmacological and Natural Alternatives to Antibiotics. Marine Science and Technology Bulletin, 13(3), 239–250. https://doi.org/10.33714/masteb.1488998 DOI: https://doi.org/10.33714/masteb.1488998
Krymskaya, A. S. (2024). Poster as an Information Product in the System of Scholarly Communications. Bibliosphere, 1, 76–84. https://doi.org/10.20913/1815-3186-2024-1-76-84 DOI: https://doi.org/10.20913/1815-3186-2024-1-76-84
Kurniasari, M., Sari, K. R. P., & Purnamaningsih, N. (2022). Antibacterial Activities of Polar Fraction of Papaya Leaf Ethanolic Extract (Carica Papaya Linn.) Against Escherichia Coli and Staphylococcus Aureus. Jurnal Penelitian Saintek, 2(27). https://doi.org/10.21831/jps.v2i27.53006 DOI: https://doi.org/10.21831/jps.v2i27.53006
Lawani-Luwaji, E. (2024). The Antibiotic Era: A Golden Age and its Challenges. British Journal of Multidisciplinary and Advanced Studies, 5(2), 57–68. https://doi.org/10.37745/bjmas.2022.0474 DOI: https://doi.org/10.37745/bjmas.2022.0474
Lishchyna, N., Boiko, L., & Hulchuk, Y. (2024). Assessment Of Risks And Their Impact On The Life Cycle Of Software Development. Herald of Khmelnytskyi National University. Technical Sciences, 343(1), 141-145. https://doi.org/10.31891/2307-5732-2024-343-6-21 DOI: https://doi.org/10.31891/2307-5732-2024-343-6-21
Masruroh, Septaria, K., & Haque, E. H. (2022). Upaya Peningkatan Pengetahuan Masyarakat Terhadap Covid-19 Menggunakan Media Poster. Ta’awun, 2(02), 115–124. https://doi.org/10.37850/taawun.v2i02.266 DOI: https://doi.org/10.37850/taawun.v2i02.266
Mehboob, R. (2022). Antibiotic Resistance: an Upcoming Pandemic. Pakistan Journal of Health Sciences. https://doi.org/10.54393/pjhs.v3i06.379 DOI: https://doi.org/10.54393/pjhs.v3i06.379
Mubarat, H., Ilhaq, M., Al Hafids, A. R., & Fajri, A. I. (2023). Prinsip Gestalt pada Komposisi Visual Poster “Ancaman Penyakit saat Musim Hujan” Produksi Dinas Kesehatan Provinsi Sumatera Selatan. Gorga : Jurnal Seni Rupa, 12(2), 386. https://doi.org/10.24114/gr.v12i2.50889 DOI: https://doi.org/10.24114/gr.v12i2.50889
Noor, N. H. M., Sulaiman, S., Kadiman, S., & Louis, N. (2023). Empowering Patients Through Informative Health Posters: Enhancing Understanding and Engagement. Journal of Cardiothoracic and Vascular Anesthesia, 37, 64. https://doi.org/10.1053/j.jvca.2023.08.113 DOI: https://doi.org/10.1053/j.jvca.2023.08.113
Pangestu, P. A., Tisngati, U., & Aristya, F. (2022a). Pengembangan Media Poster Edukasi Kesiapsiagaan Bencana untuk Siswa Sekolah Dasar. Scholarly Journal of Elementary School, 2(2), 109–115. https://doi.org/10.21137/sjes.2022.2.2.6
Pangestu, P. A., Tisngati, U., & Aristya, F. (2022b). Pengembangan Media Poster Edukasi Kesiapsiagaan Bencana untuk Siswa Sekolah Dasar. Scholarly Journal of Elementary School, 2(2), 109–115. https://doi.org/10.21137/sjes.2022.2.2.6 DOI: https://doi.org/10.21137/sjes.2022.2.2.6
Peng, Z., Wang, X., Huang, J., & Li, B. (2024). Pathogenic Escherichia Coli. In Molecular Medical Microbiology (pp. 1065–1096). Elsevier. https://doi.org/10.1016/B978-0-12-818619-0.00069-1 DOI: https://doi.org/10.1016/B978-0-12-818619-0.00069-1
Pokharel, P., Dhakal, S., & Dozois, C. M. (2023). The Diversity of Escherichia Coli Pathotypes and Vaccination Strategies Against this Versatile Bacterial Pathogen. Microorganisms, 11(2), 344. https://doi.org/10.3390/microorganisms11020344 DOI: https://doi.org/10.3390/microorganisms11020344
Pujowati, R. R. A., Fitriawati, A., & Wardani, T. S. (2024). Formulasi Sabun Cair Berbahan Baku Virgin Coconut Oil (VCO) dengan Penambahan Ekstrak Daun Pepaya Carica Papaya L. untuk Peningkatan Aktivitas Anti Bakteri Staphylococcus Epidermidis Atcc 12238. EduInovasi: Journal of Basic Educational Studies, 4(3), 1734–1751. https://doi.org/10.47467/edu.v4i3.4842 DOI: https://doi.org/10.47467/edu.v4i3.4842
Putri, N. M. S., Sutiningsih, D., & Hadi, M. (2023). Skrining Fitokimia dan Uji Antibakteri Nanopartikel Perak Ekstrak Daun Pepaya (Carica Papaya L.) terhadap Bakteri Staphylococcus Epidermidis dan Salmonella Typhi. Jurnal Bios Logos, 13(3), 141–149. https://doi.org/10.35799/jbl.v13i3.49813 DOI: https://doi.org/10.35799/jbl.v13i3.49813
Ribeiro Junior, H. L. (2025). Harnessing the Power of Scientific Research for Societal Progress. Brazilian Journal of Case Reports, 5(1). https://doi.org/10.52600/2763-583X.bjcr.2025.5.1.bjcr1 DOI: https://doi.org/10.52600/2763-583X.bjcr.2025.5.1.bjcr1
Rosales, E. T., & Ruiz, N. G. (2025). New Horizons in Microbiology: Opening Doors with the Use of TICCAD in Education. SCT Proceedings in Interdisciplinary Insights and Innovations, 3, 455. https://doi.org/10.56294/piii2025455 DOI: https://doi.org/10.56294/piii2025455
Safa’ati, Y., Meldawati, M., & Hasanah, S. N. (2024). Penyediaan poster kreatif tentang pentingnya kelas ibu hamil di wilayah kerja puskesmas sungai turak. Karunia: Jurnal Hasil Pengabdian Masyarakat Indonesia, 3(1), 07–15. https://doi.org/10.58192/karunia.v3i1.1983 DOI: https://doi.org/10.58192/karunia.v3i1.1983
Salleh, M. Z., Nik Zuraina, N. M. N., Hajissa, K., Ilias, M. I., & Deris, Z. Z. (2022). Prevalence of Multidrug-Resistant Diarrheagenic Escherichia Coli in Asia: A Systematic Review and Meta-Analysis. Antibiotics, 11(10), 1333. https://doi.org/10.3390/antibiotics11101333 DOI: https://doi.org/10.3390/antibiotics11101333
Sarkono, S. (2022). Aktivitas Antibakteri Ekstrak Daun Pepaya (Carica Papaya L.) terhadap Staphylococcus Aureus. Samota Journal of Biological Sciences, 1(1), 39–46. https://doi.org/10.29303/sjbios.v1i1.1392 DOI: https://doi.org/10.29303/sjbios.v1i1.1392
Suwono, I. (2024). Community Behavior Based on Traditional Ecological Knowledge Towards Environmental Health and Disease Prevention. Global International Journal of Innovative Research, 2(9), 2305–2316. https://doi.org/10.59613/global.v2i9.332 DOI: https://doi.org/10.59613/global.v2i9.332
Utami, N. F., Rachmawaty, D., & Arisanty, A. (2023). Uji Aktivitas Antibakteri Ekstrak Daun Pepaya (Carica Papaya L.) terhadap Pertumbuhan Bakteri Vibrio Cholerae dan Salmonella Thypi. Journal of Experimental and Clinical Pharmacy (JECP), 3(2), 132. https://doi.org/10.52365/jecp.v3i2.678 DOI: https://doi.org/10.52365/jecp.v3i2.678
Utami, N. L. (2024). Efektivitas Penerapan Media Poster dalam Pembelajaran di Sekolah: Studi Literatur. Journal of Innovation in Teaching and Instructional Media, 5(1), 310–323. https://doi.org/10.52690/jitim.v5i1.999 DOI: https://doi.org/10.52690/jitim.v5i1.999
Wang, A., Greda, D., Carroll, S., Bianchi, L. M., & Cunningham, C. D. (2022). Preparing and Giving Effective Poster Presentations. In Research during Medical Residency (pp. 249–264). CRC Press. https://doi.org/10.1201/9781003126478-16 DOI: https://doi.org/10.1201/9781003126478-16
Wibowo, S. M. P., Santoso, D. B., & Apriani, R. (2023). Pengembangan Modul Perencanaan Karier untuk Meningkatkan Keterampilan Pengambilan Keputusan Karier Siswa SMP. Jurnal Pembelajaran, Bimbingan, dan Pengelolaan Pendidikan, 3(4), 320–327. https://doi.org/10.17977/um065v3i42023p320-327 DOI: https://doi.org/10.17977/um065v3i42023p320-327
Yulius, Y. (2022). Tinjauan Unsur Visual Desain Poster Iklan Layanan Masyarakat ‘Yuk Kurangi Sampah Plastikmu.’ Visualita Jurnal Online Desain Komunikasi Visual, 10(02), 17. https://doi.org/10.34010/visualita.v10i02.6345 DOI: https://doi.org/10.34010/visualita.v10i02.6345
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