Vol. 12 No. 5 (2026): In Progress
Open Access
Peer Reviewed

Effect of Sugar Concentration on Microbiological and Physicochemical Stability of Soursop Jam

Authors

Regina Ilse Marcelina BanoEt , Amanda Priscilla Nainiti , Margarethy Rohanie Mbado , Jemmy J. S. Dethan

DOI:

10.29303/jppipa.v12i5.14521

Published:

2026-05-25

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Abstract

Soursop jam is a local fruit-based product with potential for food diversification; however, its high moisture content increases susceptibility to microbiological deterioration during room-temperature storage. This study aimed to evaluate the effect of different sugar concentrations on the microbiological, physicochemical, and sensory characteristics of soursop jam and to determine its shelf life using a critical limit and risk-based microbiological assessment approach. The novelty of this study lies in the integration of microbiological indicators with physicochemical parameters to explain biological failure during storage of local fruit-based jam products. The experiment used three sugar concentrations, namely 50%, 60%, and 70% (w/w), with duplicate analyses for each treatment. Storage observations were conducted for 20 days at room temperature (27–30 °C), while microbiological analysis referred to ISO 4833-1:2013 and SNI 3746:2008 as the critical microbiological limit. The results showed that initial Total Plate Count values ranged from 23.50 × 10³ to 34.00 × 10³ CFU g⁻¹, which remained below the permissible limit. Moisture content decreased from 48.36% to 36.80% with increasing sugar concentration, while vitamin C content increased from 7.86 to 10.48 mg/100 g. The jam with 60% sugar concentration showed the highest sensory acceptance scores for color (4.64 ± 0.42) and aroma (4.78 ± 0.40). However, visible mold and yeast growth appeared after 20 days of storage in all treatments. Based on the critical limit approach, the shelf life of soursop jam is recommended not to exceed one week under room-temperature storage conditions.

Keywords:

Food safety Microbiological stability Shelf life Soursop jam Sugar concentration

References

Almaghlouth, B. J., Alqahtani, N. K., Alnabbat, K. I., Mohamed, H. A., Alnemr, T. M., & Habib, H. M. (2023). Valorization of Second-Grade Date Fruit Byproducts and Nonstandard Sweet Potato Tubers to Produce Novel Biofortified Functional Jam. Foods, 12(9). https://doi.org/10.3390/FOODS12091906

AOAC. (2019). Official Methods of Analysis of the Association of Official Analytical Chemists Official Methods of Analysis of AOAC International. 21st Edition, AOAC, Washington DC. Retrieved from https://www.scirp.org/reference/referencespapers?referenceid=2939092

Arsyad, M. (2018). Pengaruh Konsentrasi Gula Terhadap Pembuatan Selai Kelapa Muda (Cocos nucifera L). Gorontalo Agriculture Technology Journal, 1(2), 35–45. https://doi.org/10.32662/GATJ.V1I2.424

Badan Standardisasi Nasional (BSN). (2008). SNI 3746:2008 Selai Buah. Retrieved from https://pesta.bsn.go.id/produk/detail/7438-sni37462008

Banaś, A., Korus, A., & Tabaszewska, M. (2018). Quality assessment of low-sugar jams enriched with plant raw materials exhibiting health-promoting properties. Journal of Food Science and Technology, 55(1), 408–417. https://doi.org/10.1007/S13197-017-2952-6

Belitz, H. D., Grosch, W., & Schieberle, P. (2009). Food chemistry. In Food Chemistry. Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-540-69934-7

Bengi, K. S., Sodri, A., & Martono, D. N. (2025). Analysis of Raw Material Potential Based on Leading Commodities for Sustainable Industrial Development in East Java. Jurnal Penelitian Pendidikan IPA, 11(12), 102–107. https://doi.org/10.29303/JPPIPA.V11I12.13421

Campbell, S., Greenwood, M., Prior, S., Shearer, T., Walkem, K., Young, S., Bywaters, D., & Walker, K. (2020). Purposive sampling: complex or simple? Research case examples. Journal of Research in Nursing : JRN, 25(8), 652–661. https://doi.org/10.1177/1744987120927206

Duncan, D. B. (1955). Multiple Range and Multiple F Tests. Biometrics, 11(1), 1. https://doi.org/10.2307/3001478

El Hosry, L., Elias, V., Chamoun, V., Halawi, M., Cayot, P., Nehme, A., & Bou-Maroun, E. (2025). Maillard Reaction: Mechanism, Influencing Parameters, Advantages, Disadvantages, and Food Industrial Applications: A Review. Foods, 14(11). https://doi.org/10.3390/foods14111881

Giannakourou, M. C., & Taoukis, P. S. (2021). Effect of Alternative Preservation Steps and Storage on Vitamin C Stability in Fruit and Vegetable Products: Critical Review and Kinetic Modelling Approaches. Foods, 10(11). https://doi.org/10.3390/foods10112630

Haryanto, B. (2018). Pengaruh penambahan gula terhadap karakteristik bubuk instan daun sirsak (annona muricata l.) Dengan metode kristalisasi. Jurnal Penelitian Pascapanen Pertanian, 14(3), 163. https://doi.org/10.21082/JPASCA.V14N3.2017.163-170

ISO 4833-1. (2013). Microbiology of the food chain — Horizontal method for the enumeration of microorganisms — Part 1: Colony count at 30 °C by the pour plate technique. Retrieved from https://www.iso.org/standard/53728.html

Jannah, A. A., Suryani, D., & Sukesi, T. W. (2023). Determinants of Food Safety Practices among Mothers of Toddlers in Gedongkiwo Village, Mantrijeron, Yogyakarta. Jurnal Penelitian Pendidikan IPA, 9(SpecialIssue), 324–331. https://doi.org/10.29303/jppipa.v9iSpecialIssue.6161

Jha, P. K., Hanin, A., Tiwari, B. K., & Dallagi, H. (2026). Microbial safety in cold-preserved foods: risks, regulatory gaps, and mitigation strategies. Food Control, 182, 111869. https://doi.org/10.1016/j.foodcont.2025.111869

Kawai, K., Toh, M., & Hagura, Y. (2014). Effect of sugar composition on the water sorption and softening properties of cookie. Food Chemistry, 145, 772–776. https://doi.org/10.1016/j.foodchem.2013.08.127

Kubela, L., Moniharapon, E., & Tuhumury, H. C. D. (2023). Pengaruh Konsentrasi Gula Terhadap Karakteristik Kimia Dan Organoleptik Permen Jelly Buah Tomi-Tomi (Flacourtia inermis, Roxb). Jurnal Sains Dan Teknologi Pangan, 8(1). https://doi.org/10.33772/JSTP.V8I1.29963

Lalujan, L. E., Putri, A. C., Langi, T. M., & Koapaha, T. (2025). The Effect of Mixing Rind of Watermelon (Citrullus Lanatus) and Kweni Mango (Mangifera Odorata Griff.) on the Quality of Slice Jam. Jurnal Penelitian Pendidikan IPA, 11(11), 26–34. https://doi.org/10.29303/JPPIPA.V11I11.12052

Lubis, N., Damayanti, R., & Wardani, S. (2025). Antimicrobial Activity of Eco-enzymes with Various Dilutions As A Natural Disinfectant. Jurnal Penelitian Pendidikan IPA, 11(5), 506–513. https://doi.org/10.29303/JPPIPA.V11I5.11052

Lund, M. N., & Ray, C. A. (2017). Control of Maillard Reactions in Foods: Strategies and Chemical Mechanisms. Journal of Agricultural and Food Chemistry, 65(23), 4537–4552. https://doi.org/10.1021/acs.jafc.7b00882

Mao, Y., Tian, S., Qin, Y., & Chen, S. (2022). Sensory sweetness and sourness interactive response of sucrose-citric acid mixture based on synergy and antagonism. Npj Science of Food, 6(1). https://doi.org/10.1038/S41538-022-00148-0

Meilgaard, M. C., Civille, G. V., & Carr. (2015). Sensory Evaluation Techniques. https://doi.org/10.1201/B19493

Montgomery, D. C. (2017). Design and Analysis of Experiments. John Wiley & Sons.

Mukminah, N., Azzahra, H., & Fathurohman, F. (2022). Pengaruh Konsentrasi Gula Terhadap Karakteristik Kimia dan Organoleptik Selai Carica (Carica pubescens l.). Edufortech, 7(2), 147–155. https://doi.org/10.17509/edufortech.v7i2.51335

Nistor, O. V., Bolea, C. A., Andronoiu, D. G., Cotârlet, M., & Stanciuc, N. (2021). Attempts for developing novel sugar-based and sugar-free sea buckthorn marmalades. Molecules, 26(11). https://doi.org/10.3390/MOLECULES26113073

Pawar, S. V., Saldinger, S. S., & Shemesh, M. (2026). Postbiotics for improving food quality and safety: From mechanistic insights to industrial applications. LWT, 241(11), 119066. https://doi.org/10.1016/j.lwt.2026.119066

Picauly, P., Marshadu, M. J., Auratu, B., Andries, Y., Rumahrenu, M. J., Siatam, L., Erubun, B., Hamzah, W., & Wailisa, A. (2025). Pengaruh Konsentrasi Gula Terhadap Karakteristik Fisikokimia dan Organoleptik Marmalade Pisang Tongka Langit. Jurnal Teknologi Pangan Dan Industri Perkebunan (LIPIDA), 5(2), 102–108. https://doi.org/10.58466/LIPIDA.V5I2.1903

Quitral, V., Valdés, J., Umaña, V., Gallardo, N., Alcaino, M. J., Araya, C., & Flores, M. (2019). The role of non-caloric sweeteners in sensory characteristics of pastry products. Foods, 8(8). https://doi.org/10.3390/FOODS8080329

Rababah, T. M., Al-U’Datt, M., Al-Mahasneh, M., Yang, W., Feng, H., Ereifej, K., Kilani, I., & Ishmais, M. A. (2014). Effect of Jam processing and storage on phytochemicals and physiochemical properties of cherry at different temperatures. Journal of Food Processing and Preservation, 38(1), 247–254. https://doi.org/10.1111/j.1745-4549.2012.00770.x

Rahayu, W. E., Mukminah, N., & Aprilliani, F. (2026). Chemical and Organoleptic Characteristics of Pineapple Jam Production with Varying Sugar and Pectin Concentrations as Mochi Filling. Jurnal Penelitian Pendidikan IPA, 12(2), 655–661. https://doi.org/10.29303/JPPIPA.V12I2.14213

Rana, M. S., Yeasmin, F., Khan, M. J., & Riad, M. H. (2021). Evaluation of quality characteristics and storage stability of mixed fruit jam. Food Research, 5(1), 225–231. https://doi.org/10.26656/FR.2017.5(1).365

Ridawati, Alsuhendra, & Ramadani, M. I. (2023). Sanitation Knowledge and Hygiene Practices: An Evaluation of Indonesian Online Food SMEs Based on National Food Safety Guidelines. Jurnal Penelitian Pendidikan IPA, 9(12), 11641–11647. https://doi.org/10.29303/JPPIPA.V9I12.5622

Rochmah, M. M., Ferdyansyah, M. K., Nurdyansyah, F., Muliani, R., Ujianti, D., & Pangan, J. T. (2019). Pengaruh Penambahan Hidrokoloid Dan Konsentrasi Sukrosa Terhadap Karakteristik Fisik Dan Organoleptik Selai Lembaran Pepaya (Carica Papaya L.). Jurnal Pangan Dan Agroindustri, 7(4), 42–52. https://doi.org/10.21776/UB.JPA.2019.007.04.5

Rosadalima, T., Awi, E., Prasanto Bimantio, M., & Hastuti, S. (2022). Inovasi Produk Selai Salak (Salacca Zalacca) Dengan Penambahan Daun Kelor (Moringa Oleifera) Sebagai Sumber Kalsium. Jurnal Teknologi Pangan Dan Gizi (Journal of Food Technology and Nutrition), 21(2), 133–143. https://doi.org/10.33508/JTPG.V21I2.4166

Roza, M., Sari, M., Nurhasnah, Meria, A., Herman, M., & Meriko, L. (2025). Food Safety and Community Behavior Toward Traditional Foods in West Sumatra Tourist Destinations: A Chemical, Biological, and Science Education Perspective. Jurnal Penelitian Pendidikan IPA, 11(11), 165–174. https://doi.org/10.29303/JPPIPA.V11I11.12383

Scrob, T., Varodi, S. M., Vintilă, G. A., Casoni, D., & Cimpoiu, C. (2022). Estimation of degradation kinetics of bioactive compounds in several lingonberry jams as affected by different sweeteners and storage conditions. Food Chemistry: X, 16, 100471. https://doi.org/10.1016/j.fochx.2022.100471

Simamora, C., Ayu Kadek Diah Puspawati, G., Agung Istri Sri Wiadnyani, A., Studi Teknologi Pangan, P., & Teknologi Pertanian, F. (2026). Karakteristik Fisikokimia, Sensori, dan Potensi Fungsional Flakes dari Tepung Komposit Mocaf dan Kelor dengan Penambahan Tepung Pisang Kepok. Jurnal Teknologi Dan Industri Pangan, 37(1), 11–24. https://doi.org/10.6066/JTIP.2026.37.1.11

SNI 3746. (2008). Selai Teknologi & Rekayasa. Retrieved from https://www.scribd.com/doc/144557005/SNI-3746-2008-Selai

Susilowati, A., Aspiyanto, A., Dinoto, A., & Lotulunga, P. D. (2012). Pemisahan Eksopolisakarida (Eps) Sebagai Metabolit Bakteri Usus untuk Aditif Makanan dalam Biomassa Pati Sagu {Metroxylon sp.) dan Glukosa melalui Sistem Mikrofiltrasi Sel Berpengaduk (Separation of Exopolysaccharides (Eps) As Colon Bacteria Metabolismf. Jurnal Pangan, 21(4), 375–385. https://doi.org/10.33964/JP.V21I4.203

Syamsiar, Kadekoh, I., Marhani, Maemunah, Rozali, R., Zainuddin, R., Salam, N., & Humaerah, N. (2025). Sugar and Fiber Content in Sorghum Seeds (Sorghum bicolor (L.) Moench) From the Application of Rice Husk Biochar and Liquid Cow Urine Organic Fertilizer. Jurnal Penelitian Pendidikan IPA, 11(7), 467–474. https://doi.org/10.29303/JPPIPA.V11I7.11550

Taiwo, O. R., Onyeaka, H., Oladipo, E. K., Oloke, J. K., & Chukwugozie, D. C. (2024). Advancements in Predictive Microbiology: Integrating New Technologies for Efficient Food Safety Models. International Journal of Microbiology, 6612162. https://doi.org/10.1155/2024/6612162

Vermelho, A. B., Moreira, J. V., Junior, A. N., da Silva, C. R., Cardoso, V. da S., & Akamine, I. T. (2024). Microbial Preservation and Contamination Control in the Baking Industry. Fermentation, 10(5). https://doi.org/10.3390/fermentation10050231

Zaitun. (2024). Culinary Hygiene and Sanitation: A Systematic Literature Review. Jurnal Penelitian Pendidikan IPA, 10(8), 598–605. https://doi.org/10.29303/JPPIPA.V10I8.8307

Zeng, X., Meng, J., Zhang, W., He, L., Deng, L., & Ye, C. (2021). Changes in the microbiological, physicochemical properties of Chinese traditional fermented Suan rou at ripening fermentation. Food Science and Nutrition, 9(11), 5899–5913. https://doi.org/10.1002/FSN3.2095

Zhang, L., Zhang, J., & Guo, J. (2024). Relationships Between Organic Acid Metabolism and the Accumulation of Sugars and Calcium in Fruits of Cerasus humilis During Different Development Stages. Plants, 13(21), 3053. https://doi.org/10.3390/plants13213053

Zhang, R. L, Zhang, M. M., Pu, Y. F., & Zhu, L. X. (2022). Evolution of nonenzymatic browning during the simulated Msalais‐production process in models of grape juice. Food Science & Nutrition, 10(7), 2132. https://doi.org/10.1002/fsn3.2829

Žlabur, J. Š., Mikulec, N., Doždor, L., Duralija, B., Galić, A., & Voća, S. (2021). Preservation of Biologically Active Compounds and Nutritional Potential of Quick-Frozen Berry Fruits of the Genus Rubus. Processes, 9(11). https://doi.org/10.3390/pr9111940

Author Biographies

Regina Ilse Marcelina BanoEt, Universitas Kristen Artha Wacana

Author Origin : Indonesia

Amanda Priscilla Nainiti, Nusa Cendana University

Author Origin : Indonesia

Margarethy Rohanie Mbado, Artha Wacana Christian University

Author Origin : Indonesia

Jemmy J. S. Dethan, Artha Wacana Christian University

Author Origin : Indonesia

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How to Cite

BanoEt, R. I. M., Nainiti, A. P., Mbado, M. R., & Dethan, J. J. S. (2026). Effect of Sugar Concentration on Microbiological and Physicochemical Stability of Soursop Jam. Jurnal Penelitian Pendidikan IPA, 12(5), 136–145. https://doi.org/10.29303/jppipa.v12i5.14521