The Role of Indonesian Indigenous Cyanobacteria Culture Collection as An Ex-situ Conservation Effort and Microalgae Biodiversity Study Material

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DOI:

10.29303/jppipa.v9i3.2763

Published:

2023-03-31

Issue:

Vol. 9 No. 3 (2023): March

Keywords:

Cyanobacteria, Ex-situ conservation, Identification, Microalgae, Taxonomy

Research Articles

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Prihantini, N. B. . (2023). The Role of Indonesian Indigenous Cyanobacteria Culture Collection as An Ex-situ Conservation Effort and Microalgae Biodiversity Study Material. Jurnal Penelitian Pendidikan IPA, 9(3), 1269–1276. https://doi.org/10.29303/jppipa.v9i3.2763

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Abstract

Exploratory research has been carried out on Indonesian indigenous Cyanobacteria in an effort to collect these isolates to be used as learning materials for microalgae biodiversity. Cyanobacteria are known as photosynthetic bacteria and prokaryotic microalgae. The members of the cyanobacteria found were isolated, cultured, identified, and collected. The location for sampling cyanobacteria is the UI Depok campus area which consists of water from small lakes (situ) and soil. The results showed that 22 strains of Cyanobacteria were isolated and cultured properly, and consisted of 12 genera/species, namely Merismopedia sp. (1 isolate), Synechococcus sp. (1 isolate), Limnothrix redekei (1 isolate), Oscillatoria sp.1 (1 isolate), Oscillatoria sp.2 (1 isolate), Pseudanabaena sp. (3 isolates), Nostoc sp. (4 isolates), Anabaena sp. (1 isolate), Scytonema sp. (1 isolate), Fischerella sp. (1 isolate), Stigonema sp. (1 isolate), and Hapalosiphon sp. (6 isolates). Twenty-two (22) strains were then collected and treated by subculture method. Of the 11 genera found, there are 3 genera that have been known as genera that have potential as sources of biobased materials, biofuels, biofertilizers, and others, namely Synechococcus, Nostoc, and Anabaena

References

Allen, M. M. (1973). Methods for cyanophyceae. In J. R. Stein (Ed.), Handbook of phycological methods: culture methods and growth measurements (pp. 127–138). Cambridge University Press.

Anagnostidis, K., & Komárek, J. (1988). Modern approach to the classification system of cyanophytes. Archiv Für Hydrobiologie, 3(80), 327–472. https://rb.gy/xmy1kd

Anand, N. L., Radha, R. S., Hopper, S., Ravati, G., & Subramianan, T. D. (1990). Perspectives in Phycology. Today and Tomorrow’s Printers and Publishers.

Desikachary, T. V. (1959). Cyanophyta. Indian Council of Agricultural Research.

Edmondson, W. T. (1963). Freshwater biology (2nd ed.). John Wiley and Sons Inc.

Fish, S. A., & Codd, G. A. (1994). Bioactive compound production by thermophilic and thermotolerant cyanobacteria (blue-green algae). World Journal of Microbiology & Biotechnology, 10(3), 338–341. https://doi.org/10.1007/BF00414875

Geitler, L. (1985). Automixis bei pennaten Diatomeen/Automixis in Pennate Diatoms. Plant Systematics and Evolution, 150(3), 303–306. https://www.jstor.org/stable/23673691

Graham, L. E., & L.W, W. (2000). Algae. Prentice Hall.

Hoek, C., Mann, D. G., & Jahns, H. M. (1995). Algae: An introduction to phycology. Cambridge University Press.

Mollenhauer, D. (1988). Nostoc species in the field.Archies for hydrobiology Supplemental Algolical Studies (Vol. 80, Issues 1–4, pp. 315–326). https://rb.gy/vqsrc5

N.I.E.S.-collection. (2007). List of strains: Microalgae and protozoa (6th ed.). Nissei Eblo Co. Ltd.

Nelissen, B., De Baere, R., Wilmotte, A., & De Wachter, R. (1996). Phylogenetic relationships of nonaxenic filamentous cyanobacterial strains based on 16S rRNA sequence analysis. Journal of Molecular Evolution, 42(2), 194–200. https://doi.org/10.1007/BF02198845

Nelissen, B., Van de Peer, Y., Wilmotte, A., & De Wachter, R. (1995). An early origin of plastids within the cyanobacterial divergence is suggested by evolutionary trees based on complete 16S rRNA sequences. Molecular Biology and Evolution, 12(6), 1166–1173. https://doi.org/10.1093/oxfordjournals.molbev.a040289

Nicholas, D. J. D. (1980). Mineral nutrients requirements of utilization by algal flora of freshwater lake. In Technical Paper (Issue 50). Australian Government.

Nichols, H. W. (1973). Growth media-freshwater, In. In J. R. Stein (Ed.), Handbook of phycological methods: Culture methods and growth measurements (pp. 7–24). Cambridge University Press.

Oliver, R. L., & Ganf, C. G. (2000). Freshwater bloom. In A. Whitton & M. Potts (Eds.), The ecology of Cyanobacteria (pp. 149–194). Kluwer Academic Publisher.

Pentecost, A. (1984). Introduction to fresh water algae. Richmond Publishing Co. Ltd.

Prescott, G. W. (1962). Algae of the Western Great Lake Areas. W.M.C. Brown Company Publisher.

Prihantini, N. . (2002). Flora mikroalga di perairan kampus Universitas Indonesia. Proceeding of Limnology National Seminar2002.

Prihantini, N. B., Wardhana, W., Hendrayanti, D., Widyawan, A., Ariyani, Y., & Rianto, R. (2008). Biodiversitas Cyanobacteria dari beberapa situ/danau di kawasan Jakarta-Depok-Bogor, Indonesia. Makara UI.

Rheinheimen, G. (1980). Aquatic microbiology (2nd ed.). Jhon Wiley & sons.

Rippka, R. (1988). Recognition and Identification of Cyanobacteria. Methods in Enzymology, 167(C), 28–67. https://doi.org/10.1016/0076-6879(88)67005-4

Robertson, B. R., Tezuka, N., & Watanabe, M. M. (2001). Phylogenetic analyses of Synechococcus strains (cyanobacteria) using sequences of 16S rDNA and part of the phycocyanin operon reveal multiple evolutionary lines and reflect phycobilin content. International Journal of Systematic and Evolutionary Microbiology, 51(3), 861–871. https://doi.org/10.1099/00207713-51-3-861

Santra, S. C. (1993). Biology of Ricefields blue green algae. Daya Publishing House.

Schlegel, I., Doan, N. T., De Chazal, N., & Smith, G. D. (1998). Antibiotic activity of new cyanobacterial isolates from Australia and Asia against green algae and cyanobacteria. Journal of Applied Phycology, 10(5), 471–479. https://doi.org/10.1023/A:1008042619686

Singh, R. N. (1961). Role of Blue-Green Algae in Nitrogen Economy of Indian Agriculture. Role of Blue-Green Algae in Nitrogen Economy of Indian Agriculture, 99–101. https://www.cabdirect.org/cabdirect/abstract/19631602458

Taqyuddin, J., Sirait, I., L., N., A., H., Ramelan, & Firdausy. (1997). Atlas kampus universitas indonesia. Fakultas MIPA UI.

Turner, S. (1997). Molecular systematics of oxygenic photosynthetic bacteria. Plant Systematics and Evolution Supplement, 11, 13–52. https://doi.org/10.1007/978-3-7091-6542-3_2

Uzunova-Doneva, T., & Donev, T. (2005). Anabiosis and conservation of microorganisms. Journal of Culture Collections, 4, 17–28. http://www.bioline.org.br/request?cc05002

Vashishta, B. R. (1999). Botany for degree students: algae (8 th). Chad & Company Ltd.

Vidal, L., Ballot, A., Azevedo, S. M. F. O., Padisák, J., & Welker, M. (2021). Introduction to cyanobacteria. In B. A. Whitton & P. M (Eds.), Toxic Cyanobacteria in Water (pp. 163–211). Kluwer Academic Publisher. https://doi.org/10.1201/9781003081449-3

Whitton, B. A. (2002). Phylum Cyanophyta (Cyanobacteria) (D. M. Jhon, B. A. Whitton, & A. J. Brook (eds.)). Cambridge University Press.

Wilmotte, A. (1994). Molecular Evolution and Taxonomy of the Cyanobacteria. In D. A. Bryant (Ed.), The Molecular Biology of Cyanobacteria (pp. 1–25). Kluwer Academic Publishers. https://doi.org/10.1007/978-94-011-0227-8_1

Wilmotte, A., & Herdman, M. (2015). Phylogenetic Relationships Among the Cyanobacteria Based on 16S rRNA Sequences. In D. R. Boone & R. W. Castenholz (Eds.), Criminal Behaviour and Mental Health (2nd ed., Vol. 2015, pp. 1–9). https://doi.org/10.1002/9781118960608.CBM00021

Wilmotte, A., Turner, S., Van de Peer, Y., & Pace, N. R. (1992). Taxonomic Study of Marine Oscillatoriacean Strains (Cyanobacteria) With Narrow Trichomes. Ii. Nucleotide Sequence Analysis of the 16S Ribosomal Rna. Journal of Phycology, 28(6), 828–838. https://doi.org/10.1111/j.0022-3646.1992.00828.x

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