The Effect of Protein Induction of Brachionus sp. Toward In-Vivo Response of Cluster Differentiation-8 (CD8) Cantang Grouper (Epinephelus sp.) Infected by VNN (Viral Nervous Necrosis)
DOI:
10.29303/jppipa.v9i8.4025Published:
2023-08-25Issue:
Vol. 9 No. 8 (2023): AugustKeywords:
Brachionus sp, Cantang grouper, CD8 (Cluster Differentiation-8)Research Articles
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Abstract
The large demand for grouper conduce increase production intensively in various ways, by increasing the production of grouper aquaculture raises causing new problems, one of which is infection with VNN disease. The use of antibiotics to treat VNN infections is prohibited or no longer recommended by the government. An alternative way to deal with VNN infection is by increasing the body's immunity in the fish itself. One effort that can be done to increase the body's immunity in grouper is by administering Brachionus sp. protein extracts. This research was conducted to determine whether the Brachionus sp. has an effect on the immune system in cantang grouper (Epinephelus sp.) as indicated by the emergence of Cluster Differentiation-8 (CD8) expression in fish that have been infected with VNN. The proteins contained in Brachionus sp. that has three type molecular weight (122.73 kDa, 75.49 kDa, and 13.77 kDa) potential as an ingredient for treating VNN-infected fish. It is hoped that this can improve the immune system in Epinephelus sp. infected with VNN. Protein Brachionus sp. given by injection at a dose of 35 µl, 105 µl and 170 µl per 150 gr Epinephelus sp. 105 µl was the best concentration in boosting the immune system in the tested Epinephelus sp., as indicated by a decreased CD8 count.
References
Anita, N. S., & Dewi, N. N. (2020). Evaluation of hatching rate, growth performance, and survival rate of cantang grouper (Epinephelus fuscoguttatus × lanceolatus) in concrete pond at Situbondo, East Java, Indonesia. IOP Conference Series: Earth and Environmental Science, 441(1), 012019. https://doi.org/10.1088/1755-1315/441/1/012019
Ashfaq, H., Soliman, H., Saleh, M., & El-Matbouli, M. (2019). CD4: a vital player in the teleost fish immune system. Veterinary Research, 50(1), 1. https://doi.org/10.1186/s13567-018-0620-0
Boukedjouta, R., Pretto, T., Abbadi, M., Biasini, L., Toffan, A., & Mezali, K. (2020). Viral encephalopathy and retinopathy is endemic in wild groupers (genus Epinephelus spp.) of the Algerian coast. Journal of Fish Diseases, 43(7), 801–812. https://doi.org/10.1111/jfd.13181
Castro, R., Takizawa, F., Chaara, W., Lunazzi, A., Dang, T. H., Koellner, B., Quillet, E., Six, A., Fischer, U., & Boudinot, P. (2013). Contrasted TCRβ Diversity of CD8+ and CD8− T Cells in Rainbow Trout. PLoS ONE, 8(4), e60175. https://doi.org/10.1371/journal.pone.0060175
Cauwels, A., Rogge, E., Vandendriessche, B., Shiva, S., & Brouckaert, P. (2014). Extracellular ATP drives systemic inflammation, tissue damage and mortality. Cell Death & Disease, 5(3), e1102–e1102. https://doi.org/10.1038/cddis.2014.70
Chen, N.-C., Yoshimura, M., Guan, H.-H., Wang, T.-Y., Misumi, Y., Lin, C.-C., Chuankhayan, P., Nakagawa, A., Chan, S. I., Tsukihara, T., Chen, T.-Y., & Chen, C.-J. (2015). Crystal Structures of a Piscine Betanodavirus: Mechanisms of Capsid Assembly and Viral Infection. PLOS Pathogens, 11(10), e1005203. https://doi.org/10.1371/journal.ppat.1005203
Chieng, C. C. Y., Daud, H. M., Yusoff, F. M., & Abdullah, M. (2018). Immunity, feed, and husbandry in fish health management of cultured Epinephelus fuscoguttatus with reference to Epinephelus coioides. Aquaculture and Fisheries, 3(2), 51–61. https://doi.org/10.1016/j.aaf.2018.01.003
Duval, D., Demangel, C., Munier-Jolain, K., Miossec, S., & Geahel, I. (1991). Factors controlling cell proliferation and antibody production in mouse hybridoma cells: I. Influence of the amino acid supply. Biotechnology and Bioengineering, 38(6), 561–570. https://doi.org/10.1002/bit.260380602
Gonzalez-Silvera, D., Herrera, M., Giráldez, I., & Esteban, M. (2018). Effects of the Dietary Tryptophan and Aspartate on the Immune Response of Meagre (Argyrosomus regius) after Stress. Fishes, 3(1), 6. https://doi.org/10.3390/fishes3010006
Guo, Z., Wang, G. L., Fu, J. P., & Nie, P. (2013). Characterization and expression of Cd8 molecules in mandarin fish Siniperca chuatsi. Journal of Fish Biology, 82(1), 189–205. https://doi.org/10.1111/j.1095-8649.2012.03475.x
Hamre, K. (2016). Nutrient profiles of rotifers ( Brachionus sp.) and rotifer diets from four different marine fish hatcheries. Aquaculture, 450, 136–142. https://doi.org/10.1016/j.aquaculture.2015.07.016
Jeeja., I. J., & Raj, P. (2011). Nutritional Composition Of Rotifer (Brachionus plicatilis Muller) Cultured Using Selected Natural Diets. Indian J. Fish, 58(2), 59–65. Retrieved from https://epubs.icar.org.in/index.php/IJF/article/view/7324/3447
Kelly, B., & Pearce, E. L. (2020). Amino Assets: How Amino Acids Support Immunity. Cell Metabolism, 32(2), 154–175. https://doi.org/10.1016/j.cmet.2020.06.010
Kono, T., & Korenaga, H. (2013). Cytokine Gene Expression in CD4 Positive Cells of the Japanese Pufferfish, Takifugu rubripes. PLoS ONE, 8(6), e66364. https://doi.org/10.1371/journal.pone.0066364
Maehre, H. K., Hamre, K., & Elvevoll, E. O. (2013). Nutrient evaluation of rotifers and zooplankton: feed for marine fish larvae. Aquaculture Nutrition, 19(3), 301–311. https://doi.org/10.1111/j.1365-2095.2012.00960.x
Makridis, P., & Olsen, Y. (1999). Protein depletion of the rotifer Brachionus plicatilis during starvation. Aquaculture, 174(3–4), 343–353. https://doi.org/10.1016/S0044-8486(99)00020-4
Masitha, A. (2019). In-Vivo Test of Chlorella vulgaris Extract for Induction into Heat Shock Proteins of Cantang Grouper (Ephinephelus fuscoguttatus-lanceolatus) Infected by Viral Nervous Necrosis. JFMR-Journal of Fisheries and Marine Research, 3(1), 22–31. https://doi.org/10.21776/ub.jfmr.2019.003.01.3
Mastuti, I., & K. Mahardika, D. S. (2019). Ketahanan Ikan Kerapu Hibrida Cantang (Epinephelus fuscoguttatus>https://doi.org/10.36526/biosense.v2i02.959
Munday, B. L., Kwang, J., & Moody, N. (2002). Betanodavirus infections of teleost fish: a review. Journal of Fish Diseases, 25(3), 127–142. https://doi.org/10.1046/j.1365-2761.2002.00350.x
Musdja, M. Y., Lutfie, S. H., Gustin, A., Lestari, M. S., & Sari, R. (2017). Uji Efek Imunomodulator Kapsul Campuran Komponen Menyirih (Piper betle L., Uncaria gambir Roxb., dan Ca (OH) 2) Terhadap Kadar CD4, CD8, CD19 DAN CD56 Dalam Darah. Fakultas Kedokteran dan Ilmu Kesehatan. Retrieved from http://repository.uinjkt.ac.id/dspace/handle/123456789/51812%0Ahttp://repository.uinjkt.ac.id/dspace/bitstream/123456789/51812/3/1.III.5 Uji Efek Imunomodulator Kapsul Campuran Komponen Menyirih terhadap Kadar dalam Darah.pdf
Nakanishi, T., Shibasaki, Y., & Matsuura, Y. (2015). T Cells in Fish. Biology, 4(4), 640–663. https://doi.org/10.3390/biology4040640
Srivastava, A., Hamre, K., Stoss, J., Chakrabarti, R., & Tonheim, S. K. (2006). Protein content and amino acid composition of the live feed rotifer (Brachionus plicatilis): With emphasis on the water soluble fraction. Aquaculture, 254(1–4), 534–543. https://doi.org/10.1016/j.aquaculture.2005.11.014
Tang, Q., Tan, P., Ma, N., & Ma, X. (2021). Physiological Functions of Threonine in Animals: Beyond Nutrition Metabolism. Nutrients, 13(8), 2592. https://doi.org/10.3390/nu13082592
Tizard, I. R. (2004). Veterinary Immunology: An Introduction (7th ed.). Saunders Collage Publishing.
Valencia, J., Grau, A., Pretto, T., Pons, J., Jurado-Rivera, J., Castro, J., Toffan, A., & Catanese, G. (2019). Viral encephalopathy and retinopathy (VER) disease in Epinephelus marginatus from the Balearic Islands marine protected areas. Diseases of Aquatic Organisms, 135(1), 49–58. https://doi.org/10.3354/dao03378
Yanuhar, U. (2011). The Function of Receptor Protein Humpback Grouper Cromileptes Altivelis in Expression and Proliferation of CD4 and CD8 cells in Defence Immunity of Viral Nervous Necrotic Infection. International Journal of Bioscience, Biochemistry and Bioinformatics, 9, 119–124. https://doi.org/10.7763/IJBBB.2011.V1.22
Yanuhar, U., Arfiati, D., Musa, M., Kusriani, Junirahma, N. S., & Caesar, N. R. (2020). The Status of VNN (Viral Nervous Necrosis)-lnfected Grouper Fish Tissue with Chlorella vulgaris Extract as Anti-Virus Candidate. Journal of Physics: Conference Series, 1665(1), 012036. https://doi.org/10.1088/1742-6596/1665/1/012036
Yanuhar, U., & Khumaidi, A. (2017). The application of pigment-protein fraction from Nannochloropsis oculata on β-actin response of Cromileptes altivelis infected with viral nervous necrosis. Jurnal Akuakultur Indonesia, 16(1), 22. https://doi.org/10.19027/jai.16.1.22-32
Zafran, Koesharyani, I., Johnny, F., Yuasa, K., Harada, T., & Hatai, K. (2000). Viral nervous necrosis in humpback grouper Cromileptes altivelis larvae and juveniles in Indonesia. Fish Pathology, 35(2), 95–96. https://doi.org/10.3147/jsfp.35.95
Author Biographies
Nur Fauziyah Martiningsih, Universitas Brawijaya
Uun Yanuhar, Universitas Brawijaya
Mohammad Musa, Universitas Brawijaya
Dwi Retna Kumalaningrum, Universitas Brawijaya
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Copyright (c) 2023 Nur Fauziyah Martiningsih, Uun Yanuhar, Mohammad Musa, Dwi Retna Kumalaningrum
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