Isolation and Screening Microorganisms from Black Soldier Fly Larvae (Hermetia illucens) that Producing Amylase, Protease and Cellulase

Authors

Dalia Sukmawati , Rheva Amadea , Putri Novitasari , Yohana Caroline Sihombing , Axel Mareta , Yohannes Eka Cordias Buulolo , Atin Supiyani , Muktiningsih Nurdjayadi , Diat Nurhidayat , Roshanida A. Rahman , Sulistiyani , Raden Haryo Bimo Setiarto

DOI:

10.29303/jppipa.v9i11.4516

Published:

2023-11-25

Issue:

Vol. 9 No. 11 (2023): November

Keywords:

Amylase, Black soldier fly, Cellulase, Microorganisms, Protease

Research Articles

Downloads

How to Cite

Sukmawati, D., Amadea, R. ., Novitasari, P. ., Sihombing, Y. C. ., Mareta, A. ., Buulolo, Y. E. C. ., … Setiarto, R. H. B. . (2023). Isolation and Screening Microorganisms from Black Soldier Fly Larvae (Hermetia illucens) that Producing Amylase, Protease and Cellulase . Jurnal Penelitian Pendidikan IPA, 9(11), 9784–9793. https://doi.org/10.29303/jppipa.v9i11.4516
Crossref
Scopus
Google Scholar
Europe PMC

Downloads

Metrics

PDF views
335
Nov 25 '23Nov 28 '23Dec 01 '23Dec 04 '23Dec 07 '23Dec 10 '23Dec 13 '23Dec 16 '23Dec 19 '23Dec 22 '235.0
|

Abstract

The Black Soldier Fly (BSF) has gained significant attention for its ability to decompose various types of organic waste and produce valuable enzymes related to its digestive system. These enzymes hold immense potential for applications across diverse industrial sectors, with promise in the livestock industry. This study aimed to extract and assess amylase, protease, and cellulase enzymes from microorganisms present in BSF larvae fermentation. The isolation technique employed was the spread plate method, followed by rigorous screening for the presence of these enzymes. The results showed 46 isolates of 26 bacterial isolates and 20 yeast isolates. In the amylase enzyme screening, a substantial 25 bacterial and 19 yeast isolates exhibited positive amylase activity. For cellulase, 20 bacterial and 14 yeast isolates displayed positive results. In the case of protease, 16 bacterial and 12 yeast isolates demonstrated protease enzyme activity. Notably, nine isolates exhibited the remarkable capability to produce multiple enzymes, including eight bacterial and one yeast isolate. These results showcase the rich enzymatic potential of BSF-associated microorganisms, offering exciting prospects for their application in various industrial sectors, especially in enhancing the efficiency and sustainability of livestock production

References

Aladdin, A., Alsaheb, R. A. A., Pareek, A., Othman, N. Z., & Enshasy, E. (2017). Biotechnological Aspects and Pharmaceutical Applications of Bacterial Proteases. Der Pharmacia Lettre, 9(2), 9–20. Retrieved from https://www.scholarsresearchlibrary.com/articles/pharmacology-and-toxicology-pharmaceutical-chemistry-pharmaceutical-analysis-and-quality-assurance-pharmaceutics-hospita.pdf

Arman, Z., Sondana, G. A., Fikriyyah, N. N., Afifah, Z. N., Balqis, M., Hasanah, R., Risandi, A., Sofiana, I., Nisa, H., Ridawati, Muktiningsih, M., & Sukmawati, D. (2020). Screening of amylolytic and cellulolytic yeast from Dendrobium spathilingue in Bali Botanical Garden, Indonesia. AIP Conference Proceedings, 2242(1), 050013. https://doi.org/10.1063/5.0007802

Bogianto, R. W. (2019). Karakterisasi dan Pemisahan Enzim Amilase Dari Hasil Isolasi Bakteri Amilolitik Pada Larva Black Soldier Fly. Retrieved from http://repository.itk.ac.id/125/

Darmawan, M., Sarto, S., & Prasetya, A. (2017). Budidaya Larva Black Soldier Fly (Hermetia illucens.) Dengan Pakan Limbah Dapur (Daun Singkong. Prosiding Simposium Nasional Rekayasa Aplikasi Perancangan Dan Industri Simposium Nasional Ke-16. Retrieved from https://publikasiilmiah.ums.ac.id/handle/11617/9510

Fahmi, M. R. (2015). Optimalisasi proses biokonversi dengan menggunakan mini-larva Hermetia illucens untuk memenuhi kebutuhan pakan ikan. Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia, 1(1), 139–144. https://doi.org/10.13057/psnmbi/m010124

Farooq, M. A., Ali, S., Hassan, A., Tahir, H. M., Mumtaz, S., & Mumtaz, S. (2021). Biosynthesis and industrial applications of α-amylase: a review. Archives of Microbiology, 203(4), 1281–1292. https://doi.org/10.1007/s00203-020-02128-y

Gopinath, S. C. B., Anbu, P., Arshad, M. K. M., Lakshmipriya, T., Voon, C. H., Hashim, U., & Chinni, S. V. (2017). Biotechnological Processes in Microbial Amylase Production. BioMed Research International, 1–9. https://doi.org/10.1155/2017/1272193

Gorrens, E., Van Looveren, N., Van Moll, L., Vandeweyer, D., Lachi, D., De Smet, J., & Van Campenhout, L. (2021). Staphylococcus aureus in Substrates for Black Soldier Fly Larvae (Hermetia illucens) and Its Dynamics during Rearing. Microbiology Spectrum, 9(3). https://doi.org/10.1128/spectrum.02183-21

Guder, D. G., & Krishna, M. S. R. (2019). Isolation and Characterization of Potential Cellulose Degrading Bacteria from Sheep Rumen. Journal of Pure and Applied Microbiology, 13(3), 1831–1839. https://doi.org/10.22207/JPAM.13.3.60

Hastutiek, P., & Fitri, L. E. (2013). POTENSI Musca domesticaLinn. sebagai Vektor Beberapa Penyakit. Jurnal Kedokteran Brawijaya, 23(3), 125–136. https://doi.org/10.21776/ub.jkb.2007.023.03.4

Holmes, E., Li, J. V., Marchesi, J. R., & Nicholson, J. K. (2012). Gut Microbiota Composition and Activity in Relation to Host Metabolic Phenotype and Disease Risk. Cell Metabolism, 16(5), 559–564. https://doi.org/10.1016/j.cmet.2012.10.007

Kim, C.-H., Ryu, J., Lee, J., Ko, K., Lee, J., Park, K. Y., & Chung, H. (2021). Use of Black Soldier Fly Larvae for Food Waste Treatment and Energy Production in Asian Countries: A Review. Processes, 9(1), 161. https://doi.org/10.3390/pr9010161

Liu, C., Wang, C., & Yao, H. (2019). Comprehensive Resource Utilization of Waste Using the Black Soldier Fly (Hermetia illucens (L.)) (Diptera: Stratiomyidae). Animals, 9(6), 349. https://doi.org/10.3390/ani9060349

Mahdiyah, D. (2015). Isolasi bakteri dari tanah gambut penghasil enzim protease. Jurnal Pharmascience, 2(2), 71–79. https://doi.org/10.20527/jps.v2i2.5825

Malek, K., Norazan, M., Parasuraman, R., Othman, N., Malek, R., Aziz, R., Aladdin, A., & Enshasy, H. El. (2016). Production of Cysteine Proteases by Recombinant Microorganisms : A Critical Review. IOSR Journal of Pharmacy and Biological Sciences, 11(2), 35–40. https://doi.org/10.9790/3008-1102043540

Nisa, K. (2018). Skrinning dan Aktivitas Enzim Bakteri Selulotik yang Berasal dari Mingut Larva Black Soldier Fly (Hermetia ellucens L.) (Diptera :Stratiomyidae). Retrieved from https://repository.unej.ac.id/xmlui/handle/123456789/93847

Novianti, O. (2023). Biokonversi Selulosa Pada Ampas Tebu (Bagasse) Menjadi Nanokomposit Hidrogel Dengan Memanfaatkan Enzim Hidrolitik Dari Indigenous Composting Actinomycetes. Retrieved from http://digilib.unila.ac.id/69197/

Panousi, M. N., Williams, G. J., Girdlestone, S., Hiom, S. J., & Maillard, J.-Y. (2009). Evaluation of alcohol wipes used during aseptic manufacturing. Letters in Applied Microbiology, 48(5), 648–651. https://doi.org/10.1111/j.1472-765X.2009.02574.x

Pricilia, S., Astuti, W., & Marliana, E. (2018). Skrining Bakteri Endofit Penghasil Amilase, Lipase Dan Protease Dari Daun Macaranga hullettii King ex Hook.f. Screening Bacterial Of Endophytic Producing Amylase, Lipase And Protease From Leaves Of Macaranga hullettii King ex Hook.f. Atomik, 3(2), 102–105. Retrieved from http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JA/article/view/634

Rahmiati, R., Pujianto, S., & Kusdiyantini, E. (2016). Eksplorasi Mikroba Penghasil Enzim-enzim Hidrolitik Di Kawasan Taman Nasional Lore Lindu Sulawesi Tengah. Bioma : Berkala Ilmiah Biologi, 18(2), 14. https://doi.org/10.14710/bioma.18.2.14-19

Rumondor, G., Maaruf, K., Tulung, Y. R. L., & Wolayan, F. R. (2015). Pengaruh Penggantian Tepung Ikan dengan Tepung Maggot Black Soldier (Hermetia illucens) dalam Ransum Terhadap Persentase Karkas dan Lemak Abdomen Broiler. ZOOTEC, 35(2), 131. https://doi.org/10.35792/zot.36.1.2016.10452

Sedijani, P., Rasmi, D. A. C., Kusmiyati, K., Irawati, I., & Bahri, S. (2022). Little Tuna (Euthynnus affinis) Waste-Based Medium for Fungal Growth and The Lipolytic Activity. Jurnal Penelitian Pendidikan IPA, 8(6), 2802–2807. https://doi.org/10.29303/jppipa.v8i6.2706

Souza, P. M. de, Bittencourt, M. L. de A., Caprara, C. C., Freitas, M. de, Almeida, R. P. C. de, Silveira, D., Fonseca, Y. M., Ferreira Filho, E. X., Pessoa Junior, A., & Magalhães, P. O. (2015). A biotechnology perspective of fungal proteases. Brazilian Journal of Microbiology, 46(2), 337–346. https://doi.org/10.1590/S1517-838246220140359

Sukmawati, D., Arman, Z., Hasanah, R., Balqis, M., Setiawan, A., Tafrijiyyah, F., Sari, R., Supiyani, A., Prihantini, N. B., Husna, S. N. A., El Enshasy, H. A., & Dailin, D. J. (2021). Application of yeasts isolated from fermented cocoa beans for biocontrol of pathogenic mold in chocolate fruit. Journal of Physics: Conference Series, 1869(1), 012042. https://doi.org/10.1088/1742-6596/1869/1/012042

Sukmawati, D., Larasati, R. P., Kurniati, T. H., Arman, Z., & El Enshasy, H. A. (2019). Molds isolated from chicken feed as potential amylase resources. International Journal of Scientific and Technology Research, 8(11), 188–196. Retrieved from http://www.ijstr.org/final-print/nov2019/Molds-Isolated-From-Chicken-Feed-As-Potential-Amylase-Resources.pdf

Sukmawati, D., Saidah, N., Handayani, K. T., & Rahayu, S. (2018). The characteristics of fungi contaminating chicken feed in Tegal, Bogor, West Java. Asian Journal of Agriculture and Biology, 6(4), 472–480. Retrieved from https://www.asianjab.com/wp-content/uploads/2019/01/6-AJAB-2018-05-162-The-characteristics-of-fungi-contaminating-chicken-feed-in-Tegal-Bogor-West-Java.pdf

Sukmawati, D., Wahyudi, P., Rahayu, S., Moersilah, M., Handayani, T., Rustam, K. Y., & Puspitasari, S. I. (2018). Skrining Kapang Aspergillus spp. Penghasil Aflatoksin pada Jagung Pipilan di Daerah Bekasi, Jawa Barat. Al-Kauniyah: Jurnal Biologi, 11(2), 151–162. https://doi.org/10.15408/kauniyah.v11i2.6961

Sundarram, A., & Murthy, T. P. K. (2014). α -Amylase Production and Applications : A Review. Journal of Applied & Environmental Microbiology, 2(4), 166–175. https://doi.org/10.12691/jaem-2-4-10

Vertygo, S. (2021). Isolasi dan Skrining Actinomycetes endofitik pada Akar Mangrove Yang Berpotensi Menghasilkan Enzim Hidrolitik. Jurnal Pendidikan Dan Sains Biologi, 4(3), 79–91. https://doi.org/10.33323/indigenous.v4i3.264

Widiasti, M., Putra, I. W. W. P., Duniaji, A. S., & Darmayanti, L. P. (2020). Analisis Potensi Beberapa Larutan Pengencer Pada Uji Antibakteri Teh Temu Putih (Curcuma zedoaria (Berg.) Roscoe ) Terhadap Escherichia coli. Scientific Journal of Food Technology, 6(2), 117–125. Retrieved from https://ojs.unud.ac.id/index.php/pangan/article/download/63118/36028/

Wills, A. S., & Subagiyo. (2012). Isolasi dan Seleksi Bakteri Penghasil Enzim Ekstraseluler (proteolitik, amilolitik, lipolitik dan selulolitik) yang Berasal dari Sedimen Kawasan Mangrove. Jurnal Ilmu Kelautan, 17(3), 164–168. https://doi.org/10.14710/ik.ijms.17.3.164-169

Wulandari, T. P., Sukmawati, D., & Kurniati, T. H. (2018). Isolasi dan Seleksi Khamir Amilolitik Asal Buah Nangka (Artocarpus heterophyllus Lam.). BIOMA, 13(1), 37–42. https://doi.org/10.21009/Bioma13(1).5

Author Biographies

Dalia Sukmawati, Universitas Negeri Jakarta

Rheva Amadea, Universitas Negeri Jakarta

Putri Novitasari, Universitas Negeri Jakarta

Yohana Caroline Sihombing, Universitas Negeri Jakarta

Axel Mareta, Universitas Negeri Jakarta

Yohannes Eka Cordias Buulolo, Universitas Negeri Jakarta

Atin Supiyani, Universitas Negeri Jakarta

Muktiningsih Nurdjayadi, Universitas Negeri Jakarta

Diat Nurhidayat, Universitas Negeri Jakarta

Roshanida A. Rahman, Universiti Teknologi Malaysia, Johor, Malaysia

Sulistiyani, National Research, and Innovation Agency, Cibinong

Raden Haryo Bimo Setiarto, National Research, and Innovation Agency, Cibinong

License

Copyright (c) 2023 Dalia Sukmawati, Rheva Amadea, Putri Novitasari, Yohana Caroline Sihombing, Axel Mareta, Yohannes Eka Cordias Buulolo, Atin Supiyani, Muktiningsih Nurdjayadi, Diat Nurhidayat, Roshanida A. Rahman, Sulistiyani, Raden Haryo Bimo Setiarto

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).