Effectiveness of Giving Crude Extract of Red Betel Leaf (Piper crocatum) on the Hematology and Histopathology Profile of Goldfish (Cyprinus carpio) Gills Infected with Aeromonas hydrophila Bacteria

Authors

Muhammad Jusril Arief Pratama , Mohammad Fadjar , Akhsan Fikrillah Paricahya

DOI:

10.29303/jppipa.v10i12.7525

Published:

2025-01-06

Issue:

Vol. 10 No. 12 (2024): December

Keywords:

Aeromonas hydropilla, Hematology, Histopathology, P. crocatum

Research Articles

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Pratama, M. J. A., Fadjar, M., & Paricahya, A. F. (2025). Effectiveness of Giving Crude Extract of Red Betel Leaf (Piper crocatum) on the Hematology and Histopathology Profile of Goldfish (Cyprinus carpio) Gills Infected with Aeromonas hydrophila Bacteria. Jurnal Penelitian Pendidikan IPA, 10(12), 11263–11272. https://doi.org/10.29303/jppipa.v10i12.7525

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Abstract

The purpose of this study was to analyze the hematology and histopathology of the gills of carp (C. carpio) treated with active compounds of red betel leaf extract (P. crocatum). This research method used a completely randomized design (CRD). The treatments used in this study were doses of 75 mg/L, 150 mg/L, 225 mg/L, 300 mg/L, 375 mg/L, negative control (healthy fish), and positive control (positive control), (healthy fish), and positive control (chloramphenicol antibiotic). Results of hematology and histopathology studies the results of hematology and histopathology studies on the gills of carp (C. carpio) after being infected with A. hydropilla bacteria experienced stress, this can be seen from. decreased red blood cells, increased leukocytes, and damage to the gill tissue. Carp returned to normal after being treated with red betel leaf extract. The best dose obtained after treatment was a dose of 300 mg/L. The results of clinical symptom observations showed a change from abnormal to normal conditions in goldfish after being treated. The conclusion is that red betel leaf extract contains dominant compounds in the form of flavonoids and alkaloids which can be useful as a medicine for goldfish infected with A. hydropilla bacteria.

References

Afiff, F., & Amilah, S. (2017). Efektivitas Ekstrak Daun Mengkudu (Morinda citrifolia L.) Dan Daun Sirih Merah (Piper crocatum Ruiz & Pav) Terhadap Zona Hambat Pertumbuhan Staphylococcus aureus. STIGMA: Jurnal Matematika Dan Ilmu Pengetahuan Alam Unipa, 10(01), 12–16. https://doi.org/10.36456/stigma.vol10.no1.a635

Anggraini, S. P., Sasanti, A. D., & Wijayanti, M. (2017). Pencegahan Infeksi Aeromonas hydrophila Pada Ikan Patin (Pangasius sp.) Menggunakan Tepung Paci-Paci (Leucas lavandulaefolia) Dengan Dosis Yang Berbeda. Jurnal Akuakultur Rawa Indonesia, 5(1), 109–119. https://doi.org/10.36706/jari.v5i1.5823

Astuti, A. P. K., Hastuti, S., & Haditomo, A. H. C. (2017). Pengaruh ekstrak temulawak pada pakan sebagai imunostimulan pada ikan tawes (Puntius javanicus) dengan uji tantang bakteri. Journal of AAquaculture Management and Technology, 6(3), 10–19. Retrieved from https://ejournal3.undip.ac.id/index.php/jamt/article/view/20393

Awad, E., & Awaad, A. (2017). Role of medicinal plants on growth performance and immune status in fish. Fish & Shellfish Immunology, 67(32), 40–54. https://doi.org/10.1016/j.fsi.2017.05.034

Castilhos, R. V, Grützmacher, A. D., & Coats, J. R. (2018). Acute Toxicity and Sublethal Effects of Terpenoids and Essential Oils on the Predator Chrysoperla externa (Neuroptera: Chrysopidae). Neotropical Entomology, 47(2), 311–317. https://doi.org/10.1007/s13744-017-0547-6

Dontriska, Sasanti, A. D., & Yulisman. (2014). Efektivitas Tepung Jintan Hitam (Nigella sativa) Untuk Mencegah Infeksi Aeromonas hydrophila Pada Ikan Patin. Jurnal Akuakultur Rawa Indonesia, 2(2), 188–201. https://doi.org/10.36706/jari.v2i2.2103

Fallah, M., Kavand, A., & Mashouf, R. Y. (2014). Infected Hydatid Cysts Bacteria in Slaughtered Livestock and Their Effects on Protoscoleces Degeneration. Jundishapur Journal of Microbiology, 7(6), 1–5. https://doi.org/10.5812/jjm.10135

Gerrine, G., Prajitno, A., Fadjar, M., & Kenitasari, R. E. (2023). Effects of Keji Beling (Strobilanthes crispus) Crude Leaves Extract Against Aeromonas hydrophila Bacterial Infection in Vitro. Jurnal Penelitian Pendidikan IPA, 9(5), 3849–3855. https://doi.org/10.29303/jppipa.v9i5.3502

Gusman, E., & Firdaus, M. (2014). Pemanfaatan buah mangrove sebagai campuran pakan untuk meningkatkan pertumbuhan ikan mas. Jurnal Harpodon Borneo, 07(01), 27–35. https://doi.org/10.35334/harpodon.v7i1.4

Hastjarjo, T. D. (2019). Rancangan Eksperimen-Kuasi. Buletin Psikologi, 27(2), 187. https://doi.org/10.22146/buletinpsikologi.38619

Hikmawanti, N. P. E., Fatmawati, S., & Asri, A. W. (2021). The Effect of Ethanol Concentrations as The Extraction Solvent on Antioxidant Activity of Katuk (Sauropus androgynus (L.) Merr.) Leaves Extracts. IOP Conference Series: Earth and Environmental Science, 755(1), 012060. https://doi.org/10.1088/1755-1315/755/1/012060

Jusril, M., Prajitno, A., & Fadjar, M. (2024). Effectiveness of Administering Red Belt Leaf Extract (Piper Crocatum) Against Aeromonas Hydrophila Bacteria in Vitro. Jurnal Penelitian Pendidikan IPA, 10(3), 1025–1031. https://doi.org/10.29303/jppipa.v10i3.6074

Kenitasari, R. E., Prajitno, A., Wilujeng, A., & Gerrine, G. (2023). Potential Use of Saga Leaf Extract (Abrus precatorius) as Anti Bacteria Aeromonas hydrophila by in Vitro. Jurnal Penelitian Pendidikan IPA, 9(9), 7533–7538. https://doi.org/10.29303/jppipa.v9i9.3440

Kurniawan, A., Hermawan, R., & Syarif, A. F. (2022). Effectivity of canbat herb from pt. Meddia herbal to aeromonas hydrophilla infection on nile fish (Oreochromis sp.). Journal of Aquatropica Asia, 7(1), 34–42. https://doi.org/10.33019/joaa.v7i1.3299

Kusrini, E., Nuryati, S., Zubaidah, S., Lili Sholihah, dan, Riset Budidaya Ikan Hias Jl Perikanan No, B., & Mas, P. (2019). Pemberian Vaksin Dna Anti-Khv Ikan Mas Dengan Dosis Berbeda Terhadap Benih Ikan Koi. Jurnal Riset Akuakultur, 14(2), 95–108. https://doi.org/10.15578/jra.14.2.2019.95-108

Labh, S. N., & Shakya, S. R. (2014). Application of immunostimulants as an alternative to vaccines for health management in aquaculture. International Journal of Fisheries and Aquatic Studies, 2(1), 153–156. Retrieved from https://www.fisheriesjournal.com/vol2issue1/52.1.html

Maharani, D. E., Lumbessy, S. Y., & Azhar, F. (2022). Antibacterial Effectiveness Of Black Quick (Nigella sativa Linn) Extract On Bacteria Inhibitory Aeromonas hydrophila, And Morphology Fish Indigo (Oreochromis niloticus) Post Infection. Journal of Fish Health, 2(2), 71–82. https://doi.org/10.29303/jfh.v2i2.1410

Maisyaroh, L. A., Susilowati, T., Haditomo, A. H. C., Yuniarti, T., & Basuki, F. (2018). penggunaan ekstrak kulit buah manggis (G. mangostana) sebagai antibakteri untuk mengobati infeksi aeromonas hydrophila pada ikan nila (O. niloticus. Sains Akuakultur Tropis: Indonesian Journal of Tropical Aquaculture, 2(2), 36–43. https://doi.org/10.14710/sat.v2i2.3021

Maryani, M., & Rosdiana, R. (2020). Peranan Imunostimulan Akar Kuning Arcangelisia Flava Merr Pada Gambaran Aktivasi Sistem Imun Ikan Mas (Cyprinus carpio L). Jurnal Akuakultur Rawa Indonesia, 8(1), 22–36. https://doi.org/10.36706/jari.v8i1.10328

Nasir, M., & Khalil, M. (2016). Pengaruh penggunaan beberapa jenis filter alami terhadap pertumbuhan, sintasan dan kualitas air dalam pemeliharaan ikan mas (Cyprinus carpio). Acta Aquatica: Aquatic Sciences Journal, 3(1), 33. https://doi.org/10.29103/aa.v3i1.336

Nugrahani, R., Andayani, Y., & Hakim, A. (2016). Skrining Fitokimia Dari Ekstrak Buah Buncis (Phaseolus vulgaris L) Dalam Sediaan Serbuk. Jurnal Penelitian Pendidikan IPA, 2(1). https://doi.org/10.29303/jppipa.v2i1.38

Prasetio, E., Yanto, H., & . A. (2021). Pengaruh Ekstrak Asam Humat Tanah Gambut Terhadap Hematologi Ikan Nila (Oreochromis niloticus) Yang Diuji Tantang Bakteri Aeromonas hydrophila. Jurnal Ruaya: Jurnal Penelitian Dan Kajian Ilmu Perikanan Dan Kelautan, 9(1), 39–48. https://doi.org/10.29406/jr.v9i1.2609

Priyanka, A. K., & Smruthi, S. S. (2020). WebApplication Vulnerabilities:Exploitation and Prevention. 2020 Second International Conference on Inventive Research in Computing Applications (ICIRCA), 729–734. https://doi.org/10.1109/ICIRCA48905.2020.9182928

Putra, G. P. (2015). Pengaruh Pemberian Ekstrak Temulawak (Curcuma Xanthorrhiza ROXB) Terhadap Mortalitas Dan Gambaran Darah Benih Ikan Nilem (Osteochilus Hasselti) Dengan Uji Tantang Menggunakan Bakteri Aeromonas Hydrophila. Jurnal Mina Sains, 1(2), 68–79. https://doi.org/10.30997/jms.v1i2.12

Rahmawati, A. S., & Erina, R. (2020). Rancangan Acak Lengkap (RAL) dengan Uji Anova Dua Jalur. OPTIKA: Jurnal Pendidikan Fisika, 4(1), 54–62. https://doi.org/10.37478/optika.v4i1.333

Rand, E. E. du, Smit, S., Beukes, M., Apostolides, Z., Pirk, C. W. W., & Nicolson, S. W. (2015). Detoxification mechanisms of honey bees (Apis mellifera) resulting in tolerance of dietary nicotine. Scientific Reports, 5(1), 11779. https://doi.org/10.1038/srep11779

Shabirah, P., Harianto, & Suryana, A. (2022). The Influence of Female Labor on Rice Farming Efficiency. Jurnal Agribisains, 8(2), 54–66. https://doi.org/10.30997/jagi.v8i2.6088

Sihite, E. R., Rosmaiti, Putriningtias, A., & Putra AS, A. (2020). Pengaruh Padat Tebar Tinggi Terhadap Kualitas Air Dan Pertumbuhan Ikan Mas (Cyprinus carpio) Dengan Penambahan Nitrobacter. Jurnal Ilmiah Samudra Akuatika, 4(1), 10–16. https://doi.org/10.33059/jisa.v4i1.2444

Sumsanto, M., Yanuhar, U., & Maizar, A. (2019). Efek Pemberian Deltamethrin Terhadap Ekspresi Cd4. Journal of Fisheries Science and Technology, 15(2), 94–98. https://doi.org/10.14710/ijfst.15.2.94-98

Wahjuningrum, D., Solikhah, E. H., Budiardi, T., & Setiawati, M. (2013). Infection control of Aeromonas hydrophila in catfish (Clarias sp.) using mixture of meniran Phyllanthus niruri and garlic Allium sativum in feed. Jurnal Akuakultur Indonesia, 9(2), 93. https://doi.org/10.19027/jai.9.93-103

Yuatiati, A., Herawati, T., & Nurhayati, A. (2015). Diseminasi Penggunaan Ovaprim Untuk Mempercepat Pemijahan Ikan Mas Di Desa Sukamahi Dan Sukagalih Kecamatan Sukaratu Kabupaten Tasikmalaya Provinsi Jawa Barat. Dharmakarya, 4(1). https://doi.org/10.24198/dharmakarya.v4i1.9025

Author Biographies

Muhammad Jusril Arief Pratama, Brawijaya University, Malang

Mohammad Fadjar, Brawijaya University, Malang

Akhsan Fikrillah Paricahya, Brawijaya University, Malang

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Copyright (c) 2024 Muhammad Jusril Arief Pratama, Mohammad Fadjar, Akhsan Fikrillah Paricahya

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