Antimicrobial Activity Test of Methanol Extract of Haliclona sp. from Sibolga Waters
DOI:
10.29303/jppipa.v10i11.8287Published:
2024-11-25Downloads
Abstract
Haliclona sp. is a genus of demosponges in the family Chalinidae. Demospongiae are a type of sponge that lives in marine environments and is known for its unique, often branching skeletal structure. The use of antibiotics has led to the selection of pathogenic microorganisms in humans that are more resistant to many types of drugs, resulting in poorer treatment outcomes in cases of infection. Bacteria can become resistant to antibiotics due to improper dosing, inaccurate diagnosis, and inappropriate use of antibiotics against the causative bacteria. This study aims to determine the antimicrobial activity of the methanol extract of Haliclona sp. as an inhibitor of pathogenic microbes. This research employs a descriptive method with antagonistic testing. The results of the methanol extraction process of the symbiotic bacteria in Haliclona sp. produced a thick, dark brown extract. The results of the antagonistic test showed that the clear zones formed on the three test microbes were categorized as strong.
Keywords:
Antimicrobial Haliclona sp. MethanolReferences
Arias, C. A., & Murray, B. E. (2009). Antibiotic-Resistant Bugs in the 21st Century–A Clinical Super-Challenge. N. Engl. J. Med., 360, 439–443. https://doi.org/10.1056/NEJMp0804651
Cappucino, J. G., & Sherman, N. (1998). Microbiology, A Laboratory Manual. California: Benjamin/Cummings Science Publishing.
Davis, W. W., & Stout, T. R. (1971). Disc Plate Method of Microbiological Antibiotic Assay: I. Factors Influencing Variability and Error. Applied Microbiology, 22(4), 659-665. https://doi.org/10.1128/am.22.4.659-665.1971
Gunathilake, V., Bertolino, M., Bavestrello, G., & Udagama, P. (2020). Immunomodulatory Activity of the Marine Sponge, Haliclona (Soestella) sp. (Haplosclerida: Chalinidae), from Sri Lanka in Wistar Albino Rats: Immunosuppression and Th1-Skewed Cytokine Response. Journal of Immunology Research, 7281295. https://doi.org/10.1155/2020/7281295
Gutiérrez‐Barranquero, J. A., Reen, F. J., Parages, M. L., McCarthy, R., Dobson, A. D., & O'Gara, F. (2019). Disruption of N‐Acyl‐Homoserine Lactone‐Specific Signalling and Virulence in Clinical Pathogens by Marine Sponge Bacteria. Microbial Biotechnology, 12(5), 1049-1063. https://doi.org/10.1111/1751-7915.12867
Högberg, L. D., Heddini, A., & Cars, O. (2010). The Global Need for Effective Antibiotics: Challenges and Recent Advances. Trends Pharmacol. Sci., 31, 509–515. https://doi.org/10.1016/j.tips.2010.08.00
Hoppers, J. A., Stoudenmire, S., Wu, N. B., & Lopanik, L. (2015). Antibiotic Activity and Microbial Community of the Temperate Sponge, Haliclona sp. Journal of Applied Microbiology, 118(2), 419–430. https://doi.org/10.1111/jam.12709
Katzung, B. G. (2004). Farmakologi Dasar dan Klinik Buku 3 Edisi 8. Penerjemah dan Editor: Bagian Farmakologi FK UNAIR. Surabaya: Penerbit Salemba Medika.
Kumar, H., Singh, M. K., Gupta, M. P., & Madaan, J. (2020). Moving Towards Smart Cities: Solutions that Lead to the Smart City Transformation Framework. Technological Forecasting and Social Change, 153(C). https://doi.org/10.1016/j.techfore.2018.04.024
Lee, C. T., Chen, I. T., Yang, Y. T., Ko, T. P., Huang, Y. T., Huang, J. Y., Huang, M. F., Lin, S. J., Chen, C. Y., Lin, S. S., Lightner, D. V., Wang, H. C., Wang, A. H., Wang, H. C., Hor, L. I., & Lo, C. F. (2015). The Opportunistic Marine Pathogen Vibrio parahaemolyticus Becomes Virulent by Acquiring a Plasmid That Expresses a Deadly Toxin. Proceedings of the National Academy of Sciences of the United States of America, 112(34), 10798–10803. https://doi.org/10.1073/pnas.1503129112
Lenski, R. E. (1998). Bacterial Evolution and the Cost of Antibiotic Resistance. International Microbiology: The Official Journal of the Spanish Society for Microbiology, 1(4), 265–270.
Marzuki, I. (2018). Eksplorasi Spons Indonesia: Seputar Kepulauan Spermonde. https://doi.org/10.17605/osf.io/m5dws
Murniasih, G., Handayani, D., & Alamin, T. (2018). Proses Domestifikasi Perempuan dalam Budaya Arab (Analisis Framing Model Zhongdang Pan dan Gerald M. Kosicki dalam Film Wadjda). Jurnal Mediakita: Jurnal Komunikasi dan Penyiaran Islam, 20(1), 1-15. https://doi.org/10.30762/mediakita.v2i1.978
Murniasih, T., & Rasyid, A. (2010). Potensi Bakteri yang Berasosiasi dengan Spons Asal Barrang Lompo (Makassar) sebagai Sumber Bahan Antibakteri. Oseanologi dan Limnologi di Indonesia, 36(3), 281- 292.
Negara, K. S. (2014). Analisis Implementasi Kebijakan Penggunaan Antibiotika Rasional Mencegah Resistensi Antibiotika di RSUP Sanglah Denpasar: Studi Infeksi Methicillin Resistant Staphylococcus Aureus. Jurnal ARSI, 1(1), 42-50. https://doi.org/10.7454/arsi.v1i1.2169
Pastra, D. A., & Surbakti, H. (2012). Penapisan Bakteri yang Bersimbiosis dengan Spons Jenis Aplysina sp sebagai Penghasil Antibakteri dari Perairan Pulau Tegal Lampung. Maspari Journal: Marine Science Research, 4(1), 77-82. https://doi.org/10.56064/maspari.v4i1.1344
Pereira, R., Fontenelle, R. O. D. S., Brito, E. H. S. D., & Morais, S. M. D. (2021). Biofilm of Candida albicans: Formation, Regulation and Resistance. Journal of Applied Microbiology, 131(1), 11–22. https://doi.org/10.1111/jam.14949
Retnowati, Y., & Katili, A. S. (2023). Antibacterial Activity of Sponge-Associated Bacteria from Torosiaje Marine Area, Gorontalo, Indonesia. Biodiversitas, 24(2), 1151–1156. https://doi.org/10.13057/biodiv/d240255
Rotty, J. D., Wu, C., Haynes, E. M., Suarez, C., Winkelman, J. D., Johnson, H. E., ... & Bear, J. E. (2015). Profilin-1 Serves as a Gatekeeper for Actin Assembly by Arp2/3-dependent and-Independent Pathways. Developmental Cell, 32(1), 54-67. https://doi.org/10.1016/j.devcel.2014.10.026
Sugireng, S., &Lio, T. M. P. (2020). Penapisan Bakteri Penghasil Senyawa Metabolit Anti-Mrsa yang Bersimbiosis dengan Holothuria Scabra Asal Perairan Tanjung Tiram. Jurnal Biologi Makassar, 5(1), 34–39. https://doi.org/10.20956/bioma.v5i1.8906
Sunny, N. E., Kalavalapalli, S., Bril, F., Garrett, T. J., Nautiyal, M., Mathew, J. T., Williams, C. M., & Cusi, K. (2015). Cross-Talk between Branched-Chain Amino Acids and Hepatic Mitochondria is Compromised in Nonalcoholic Fatty Liver Disease. American Journal of Physiology-Endocrinology and Metabolism, 309(4), E311-E319. https://doi.org/10.1152/ajpendo.00161.2015
Wisplinghoff, H., Bischoff, T., Tallent, S. M., Seifert, H., Wenzel, R. P., & Edmond, M. B. (2004). Nosocomial Bloodstream Infections in US Hospitals: Analysis of 24,179 Cases from a Prospective Nationwide Surveillance Study. Clinical Infectious Diseases, 39(3), 309-317. https://doi.org/10.1086/421946
License
Copyright (c) 2024 Ulfa Mashitah, Dwi Suryanto, Kiki Nurtjahja
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
