The Use of Various Concentrations of EM4 (Effective Microorganism-4) with Moringa Leaves (Moringa oleifera) as Animal Feed to Reduce Ammonia Levels in Broiler Chicken Manure and Its Implementation in Environmental Pollution Materials
DOI:
10.29303/jppipa.v11i6.9962Published:
2025-06-25Downloads
Abstract
This study was motivated by the pungent odor of broiler manure around the farm. Chicken manure that accumulates in chicken coops and accumulates in large quantities will produce harmful gases such as ammonia. Therefore, an alternative solution is needed to manage chicken manure so that the ammonia content is not high. One process that can be used is to provide EM4 and Moringa leaves as animal feed. The purpose of this study was to determine the concentration of EM4 and Moringa leaves that can stabilize ammonia levels in broiler manure. This research is an experimental study using a group randomized design consisting of 4 treatments and each treatment used 1 kg of animal feed and each was given 2 replicates for 4 weeks. With data analysis techniques using the Anova test and the Honest Real Difference test. The design is as follows: A0: 1 kg of animal feed without EM4 and moringa leaves, A1: 1 kg of animal feed with 15 ml EM4 and 9 gr moringa leaves, A2: 1 kg of animal feed with 25 ml EM4 and 9 gr moringa leaves, A3: 1 kg of animal feed with 35 ml EM4 and 9 g moringa leaves. The results of the research conducted obtained that the concentration of EM4 with moringa leaves as the right animal feed is 25 ml EM4 and 9 gr moringa leaves (A2) where the result is the ammonia level of 10 ppm.
Keywords:
Ammonia, EM4, Moringa leafReferences
Abalansa, S., El Mahrad, B., Icely, J., & Newton, A. (2021). Electronic Waste, an Environmental Problem Exported to Developing Countries: The GOOD, the BAD and the UGLY. Sustainability, 13(9), 5302. https://doi.org/10.3390/su13095302
Al-Kerwi, M. S. M., Mardenli, O., Jasim, M. R. M., & Al-Majeed, M. A. (2022). Effects of Harmful Gases Emitted from Poultry Houses on Productive and Health Performance. IOP Conference Series: Earth and Environmental Science, 1060(1), 012082. https://doi.org/10.1088/1755-1315/1060/1/012082
Amad, A. A., & Zentek, J. (2023). The Use of Moringa oleifera in Ruminant Feeding and Its Contribution to Climate Change Mitigation. Frontiers in Animal Science, 4, 1137562. https://doi.org/10.3389/fanim.2023.1137562
Andersson, M., Pernold, K., Lilja, N., Frias-Beneyto, R., & Ulfhake, B. (2024). Longitudinal Study of Changes in Ammonia, Carbon Dioxide, Humidity and Temperature in Individually Ventilated Cages Housing Female and Male C57BL/6N Mice during Consecutive Cycles of Weekly and Bi-Weekly Cage Changes. Animals, 14(18), 2735. https://doi.org/10.3390/ani14182735
Apalowo, O. O., Ekunseitan, D. A., & Fasina, Y. O. (2024). Impact of Heat Stress on Broiler Chicken Production. Poultry, 3(2), 107–128. https://doi.org/10.3390/poultry3020010
Awewomom, J., Dzeble, F., Takyi, Y. D., Ashie, W. B., Ettey, E. N. Y. O., Afua, P. E., Sackey, L. N. A., Opoku, F., & Akoto, O. (2024). Addressing Global Environmental Pollution Using Environmental Control Techniques: A Focus on Environmental Policy and Preventive Environmental Management. Discover Environment, 2(1), 8. https://doi.org/10.1007/s44274-024-00033-5
Berg, G. V. D., & Plessis, E. D. (2023). ChatGPT and Generative AI: Possibilities for Its Contribution to Lesson Planning, Critical Thinking and Openness in Teacher Education. Education Sciences, 13(10), 998. https://doi.org/10.3390/educsci13100998
Boonmee, T., Deevong, P., Rinthong, P.-O., & Yuangsoi, B. (2024). Improvement of Nutritive Value of Soybean Meal by Microbial Hydrolysis with Bacillus subtilis Hs-2 for Use as Raw Material in Nile Tilapia (Oreochromis niloticus) Diet. Aquaculture Reports, 35, 101943. https://doi.org/10.1016/j.aqrep.2024.101943
Chaachouay, N., & Zidane, L. (2024). Plant-Derived Natural Products: A Source for Drug Discovery and Development. Drugs and Drug Candidates, 3(1), 184–207. https://doi.org/10.3390/ddc3010011
Chen, L., Han, M., Xu, J., Cao, Z., Chen, W., Jing, B., Peng, G., Wang, Y., Liao, X., Wu, Y., & Wen, X. (2024). Firmicutes Primarily Drive Odor Emission Profiles in Poultry Manure Treatments. Poultry Science, 103(11), 104250. https://doi.org/10.1016/j.psj.2024.104250
Costantino, A., Fabrizio, E., Villagrá, A., Estellés, F., & Calvet, S. (2020). The Reduction of Gas Concentrations in Broiler Houses Through Ventilation: Assessment of the Thermal and Electrical Energy Consumption. Biosystems Engineering, 199, 135–148. https://doi.org/10.1016/j.biosystemseng.2020.01.002
Dong, M., Li, Q., Xu, F., Wang, S., Chen, J., & Li, W. (2020). Effects of Microbial Inoculants on the Fermentation Characteristics and Microbial Communities of Sweet Sorghum Bagasse Silage. Scientific Reports, 10(1), 837. https://doi.org/10.1038/s41598-020-57628-0
Donkor, A.-M., Glover, R. L. K., Addae, D., & Kubi, K. A. (2013). Estimating the Nutritional Value of the Leaves of Moringa oleifera on Poultry. Food and Nutrition Sciences, 04(11), 1077–1083. https://doi.org/10.4236/fns.2013.411140
Faize, F. A., & Akhtar, M. (2020). Addressing Environmental Knowledge and Environmental Attitude in Undergraduate Students Through Scientific Argumentation. Journal of Cleaner Production, 252, 119928. https://doi.org/10.1016/j.jclepro.2019.119928
Gržinić, G., Piotrowicz-Cieślak, A., Klimkowicz-Pawlas, A., Górny, R. L., Ławniczek-Wałczyk, A., Piechowicz, L., Olkowska, E., Potrykus, M., Tankiewicz, M., Krupka, M., Siebielec, G., & Wolska, L. (2023). Intensive Poultry Farming: A Review of the Impact on the Environment and Human Health. Science of The Total Environment, 858, 160014. https://doi.org/10.1016/j.scitotenv.2022.160014
Hafez, H. M., & Attia, Y. A. (2020). Challenges to the Poultry Industry: Current Perspectives and Strategic Future After the COVID-19 Outbreak. Frontiers in Veterinary Science, 7, 516. https://doi.org/10.3389/fvets.2020.00516
Ibrahem, M. D. (2015). Evolution of Probiotics in Aquatic World: Potential Effects, the Current Status in Egypt and Recent Prospectives. Journal of Advanced Research, 6(6), 765–791. https://doi.org/10.1016/j.jare.2013.12.004
Jenssen, E. S., & Haara, F. O. (2024). High-Quality Practicum – According to Teacher Education Students on Their Practicum at Partnership Schools. European Journal of Teacher Education, 47(5), 876–894. https://doi.org/10.1080/02619768.2024.2370892
Jiang, W., Qian, L., Zhao, Y., Lin, Y., Yang, Y., Shen, H., Ge, X., & Miao, L. (2023). The Application of Moringa oleifera Leaf Meal and Its Fermentation Products in the Diet of Megalobrama amblycephala Juveniles. Fermentation, 9(6), 577. https://doi.org/10.3390/fermentation9060577
Jiang, Y., Yao, Y., Liu, H., Zhang, S., Bai, X., Ma, X., Wang, Y., & Ren, Q. (2023). Volatile Organic Compounds Conversion Pathways and Odor Gas Emission Characteristics in Chicken Manure Composting Process. Frontiers in Ecology and Evolution, 11, 1192132. https://doi.org/10.3389/fevo.2023.1192132
Khan, R. U., Khan, A., Naz, S., Ullah, Q., Laudadio, V., Tufarelli, V., & Ragni, M. (2021). Potential Applications of Moringa oleifera in Poultry Health and Production as Alternative to Antibiotics: A Review. Antibiotics, 10(12), 1540. https://doi.org/10.3390/antibiotics10121540
Khmaissa, M., Zouari-Mechichi, H., Sciara, G., Record, E., & Mechichi, T. (2024). Pollution from Livestock Farming Antibiotics an Emerging Environmental and Human Health Concern: A Review. Journal of Hazardous Materials Advances, 13, 100410. https://doi.org/10.1016/j.hazadv.2024.100410
Koberstein-Schwarz, M., & Meisert, A. (2023). Facilitating Preservice Biology Teacher Development Through Material-Based Lesson Planning. Education Sciences, 14(1), 42. https://doi.org/10.3390/educsci14010042
Kumar, R., Verma, A., Shome, A., Sinha, R., Sinha, S., Jha, P. K., Kumar, R., Kumar, P., Shubham, S., Das, S., Sharma, P., & Prasad, P. V. V. (2021). Impacts of Plastic Pollution on Ecosystem Services, Sustainable Development Goals, and Need to Focus on Circular Economy and Policy Interventions. Sustainability, 13(17), 9963. https://doi.org/10.3390/su13179963
Leitanthem, V. K., Chaudhary, P., Maiti, S., Mohini, M., & Mondal, G. (2022). Impact of Moringa oleifera Leaves on Nutrient Utilization, Enteric Methane Emissions, and Performance of Goat Kids. Animals, 13(1), 97. https://doi.org/10.3390/ani13010097
Mace, J. L., & Knight, A. (2024). From the Backyard to Our Beds: The Spectrum of Care, Attitudes, Relationship Types, and Welfare in Non-Commercial Chicken Care. Animals, 14(2), 288. https://doi.org/10.3390/ani14020288
Misto, M., Mulyono, T., Rofi’i, I., Nurhayati, A. Y., Hariadi, Y. C., & Octaviani, L. A. (2024). Measuring the Amount of Ammonia Gas in Cattle Using the MQ137 Sensor in Rawan Village, Mayang District, and Member District with the Goal of Raising Public Health Standards. Jurnal Inovasi Sains dan Teknologi untuk Masyarakat, 2(1), 22–30. https://doi.org/10.19184/instem.v2i1.757
Olaoye, A. B., Ologunde, C. A., Molehin, O. R., & Nwankwo, I. (2021). Comparative Antioxidant Analysis of Moringa oleifera Leaf Extracts from South Western States in Nigeria. Future Journal of Pharmaceutical Sciences, 7(1), 68. https://doi.org/10.1186/s43094-021-00204-8
Pereira, J. L.S., Garcia, C., & Trindade, H. (2023). Review of Measures to Control Airborne Pollutants in Broiler Housing. In M. Eyvaz, A. Albahnasawi, & M. Y. D. Alazaiza (Eds.), Environmental Sciences (Vol. 9). IntechOpen. https://doi.org/10.5772/intechopen.110582
Redweik, G. A. J., Jochum, J., & Mellata, M. (2020). Live Bacterial Prophylactics in Modern Poultry. Frontiers in Veterinary Science, 7, 592312. https://doi.org/10.3389/fvets.2020.592312
Rifat, A. I., Bormon, C. C., Akib, M. G., Ataher, M. S., Kamruzzaman, M., Dutta, A., Das, A. K., Talukder, K., Azzam, M., Farouk, M. H., Das, R., & Mahfuz, S. (2024). Dietary Inclusion of Moringa oleifera Leaf Extracts as Alternatives to Antibiotic Growth Promoter on Live Performance, Carcase Traits, Physical Meat Quality, and Health Status of Broiler Chickens. Italian Journal of Animal Science, 23(1), 1752–1763. https://doi.org/10.1080/1828051X.2024.2429608
Saha, B. U. F., Choumessi, A. T., Ayamo, A. M., Kuagny, R. B. M., Teta, I., Nantia, E. A., & Ejoh, R. A. (2022). Nutritional Quality of Three Iron-Rich Porridges Blended with Moringa oleifera, Hibiscus sabdariffa, and Solanum aethiopicum to Combat Iron Deficiency Anemia among Children. Journal of Food Quality, 2022, 1–10. https://doi.org/10.1155/2022/4309892
Senanayake, D., Torley, P. J., Chandrapala, J., & Terefe, N. S. (2023). Microbial Fermentation for Improving the Sensory, Nutritional and Functional Attributes of Legumes. Fermentation, 9(7), 635. https://doi.org/10.3390/fermentation9070635
Sharma, R., Garg, P., Kumar, P., Bhatia, S. K., & Kulshrestha, S. (2020). Microbial Fermentation and Its Role in Quality Improvement of Fermented Foods. Fermentation, 6(4), 106. https://doi.org/10.3390/fermentation6040106
Su, B., & Chen, X. (2020). Current Status and Potential of Moringa oleifera Leaf as an Alternative Protein Source for Animal Feeds. Frontiers in Veterinary Science, 7, 53. https://doi.org/10.3389/fvets.2020.00053
Suganda, A., Fahmid, I. M., Baba, S., & Salman, D. (2024). Fluctuations and Disparity in Broiler and Carcass Price Before During and After Covid-19 Pandemic in Indonesia. Heliyon, 10(8), e29073. https://doi.org/10.1016/j.heliyon.2024.e29073
Tawfik, A., Eraky, M., Osman, A. I., Ai, P., Zhou, Z., Meng, F., & Rooney, D. W. (2023). Bioenergy Production from Chicken Manure: A Review. Environmental Chemistry Letters, 21(5), 2707–2727. https://doi.org/10.1007/s10311-023-01618-x
Widestra, R. A., Yulkifli, Y., & Samudra, E. Y. A. (2020). Preliminary Analysis of Interactive Student Worksheets Development Using the Science Process Skill Approaching the 21st Century Physics Learning. Journal of Physics: Conference Series, 1481(1), 012072. https://doi.org/10.1088/1742-6596/1481/1/012072
Wola, B. R., Rungkat, J. A., & Harindah, G. M. D. (2023). Science Process Skills of Prospective Science Teachers’ in Practicum Activity at the Laboratory. Jurnal Inovasi Pendidikan IPA, 9(1), 50–61. https://doi.org/10.21831/jipi.v9i1.52974
Zhang, L., Ren, J., & Bai, W. (2023). A Review of Poultry Waste-to-Wealth: Technological Progress, Modeling and Simulation Studies, and Economic- Environmental and Social Sustainability. Sustainability, 15(7), 5620. https://doi.org/10.3390/su15075620
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