Amino Acid Content Profile and Antioxidant Activity Test of Spirulina Platensis Bioactive Protein Extract Using DPPH Method

Authors

Tahirah Hasan , Nur Ida , Yasnidar Yasir

DOI:

10.29303/jppipa.v11i4.10916

Published:

2025-04-25

Issue:

Vol. 11 No. 4 (2025): April

Keywords:

Spirulina platensis, Antioxidant, Protein, DPPH

Research Articles

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Hasan, T., Ida, N., & Yasir, Y. (2025). Amino Acid Content Profile and Antioxidant Activity Test of Spirulina Platensis Bioactive Protein Extract Using DPPH Method. Jurnal Penelitian Pendidikan IPA, 11(4), 195–201. https://doi.org/10.29303/jppipa.v11i4.10916
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Abstract

The study of Amino Acid Content Profile and Antioxidant Activity Test of Spirulina platensis Bioactive Protein Extract with DPPH Method has been conducted. This study aims to determine the protein content and IC50 value of Spirulina platensis bioactive protein extract with DPPH method. The research method includes extraction of Spirulina platensis powder using buffer A solvent. Determination of protein content with Lowry method using Bovine Serum Albumin (BSA) as standard solution and antioxidant activity test with DPPH method. The results of the analysis of protein content of Spirulina platensis extract obtained a level of 39.51%. The antioxidant activity test against DPPH free radicals showed that Spirulina platensis protein extract has antioxidant activity with IC50 value of 599.17 ± 3.75 ppm.

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Author Biographies

Tahirah Hasan, Universitas Islam Makassar

Nur Ida, Universitas Islam Makassar

Yasnidar Yasir, Universitas Islam Makassar

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Copyright (c) 2025 Tahirah Hasan, Nur Ida, Yasnidar Yasir

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