Powerful Lipolytic Activity of Fungi Isolated from Coconut and Avocado Flesh on Different pH and Temperature
DOI:
10.29303/jppipa.v7iSpecialIssue.1261Published:
2021-12-25Downloads
Abstract
Lipase is widely applied in various fields of industries elevating its economic value. The demand for lipase keeps increasing open up working opportunities. Isolates showing lipolytic activity obtained previously are tested for their activity on different pH mediums: 7, 8, 9, and 10; and at different temperatures. The fungi are grown on SDA medium supplemented with Olive Oil, emulsifier, Tween 80, and Rhodamine B. The experiment was made in 3 replicates and is incubated at room temperature of 30oC. Lipase activity was calculated based on the clear zone around colonies observed on day 2 and day 3 after inoculation. The result shows that those isolates are highly active on various pH at RT, and the activity slightly reduces at 30oC. This result suggests that a wide range of their applications are at room temperature when pH is a limiting factor for the applications
Keywords:
Lipase Activity Coconut Avocado PH TemperatureReferences
Andualema, B., & Gessesse, A., (2012). Microbial Lipases and Their Industrial Applications: Review. Biotechnology, 11: 100-118. https://doi.org/10.3923/biotech.2012.100.118
BPPT. (2015). Ciptakan Pabrik Enzim Pertama di Indonesia, BPPT Transfer Teknologi Produksi Enzim ke PT Petrosida Gresik. Retriwved from: https://www.bppt.go.id/berita-...gresik [Indonesian]
BuÅ¡ić, A., MarÄ‘etko, N., Kundas, S., Morzak, G., Belskaya, H., IvanÄić Å antek, M., Komes, D., Novak, S., & Å antek, B. (2018). Bioethanol Production from Renewable Raw Materials and Its Separation and Purification: A Review. Food technology and biotechnology, 56(3), 289–311. https://doi.org/10.17113/ftb.56.03.18.5546
De OliveiraNatalia, R.J.V., de Sousa, M.F., Pinto, W.P., Bezerra, N.L., da Silva, G.A., & Cavalcanti de QA (2020). Seasonality affects the community of endophytic fungi in coconut (Cocos nucifera) crop leaves. Acta Bot. Bras. 34 (4). https://doi.org/10.1590/0102-33062020abb0106
Fueg, B.N., Romano,, K.G., Pinlac, C.D., & Lirio, G.A.C. (2021). Evaluation of the Antimicrobial Activity of Endophytic Fungus Isolated from Cocos nucifera (L.) Cotyledon against Medically-Important Pathogens. Journal of Biosciences and Medicines 9(01) Article ID: 106786,12 https://doi.org/10.4236/jbm.2021.91007
Gennari, F., Strebdansky, S., & Pizzio, F (1998). The Use of Biocatalyst for Industrial Application. Genetic Eng, Biotechnol. 4: 14-23
Guerrand, D. (2017). Lipase Industrial Application: Focus on Food and Agro industri. Oilseed & Fat Crop and Lipid. 24(2): D403. https://doi.org/10.1051/ocl/2017031
Hasan, F., Shah, A.A., Sundus, J. & Hameed, A. (2010). Enzymes used in detergents: Lipases. African Journal of Biotechnology. 9(31), 4836-4844.
Hisham, N.H.B., Ibrahim, M.F., Ramli, N., & Abd-Aziz, S. (2019). Production of Biosurfactant Produced from Used Cooking Oil by Bacillus sp. HIP3 for Heavy Metals Removal. Molecules (Basel, Switzerland), 24(14), 2617. https://doi.org/10.3390/molecules24142617
Kouker, G and Jeager, K-R (1987). Specific and Sensitive Plate Assay for Bacterial Lipases. Applied Science and Environmental Microbiology. 1(53): 211-213.
Kumar, A and Kanwar, SS (2012). Lipase Production in Solid-State Fermentation (SSF): Recent Developments and Biotechnological Applications. Dynamic Biochemistry, Process Biotechnology and Molecular Biology ©2012 Global Science Books
Kumari, U,, Singh, R., Ray, Tue, Rana S., Saha P., Malhotra, K., & Daniell, H. ( 2019). Validation of leaf enzymes in the detergent and textile industries: launching of a new platform technology. Plant Biotechnol J. 17(6): 1167–1182. https://doi.org/10.1111/pbi.13122
Okino-Delgado., Prado, D.Z., Facanali, R., Marques, M.M.O, Nascimento, A.S., Fernandes, C.J.D., Zambuzzi, W.F., & Fleun, L.F. (2017). Bioremediation of cooking oil waste using lipases from wastes. PLoS One. 12(10): https://doi.org/10.1371/journal.pone.0186246.
Olsen, H.S., Falholt, P. The role of enzymes in modern detergency. J Surfact Deterg 1, 555–567 (1998). https://doi.org/10.1007/s11743-998-0058-7
Patel, M.T., Kilara, A., & Nagarajan, R. (1996). Lipase-catalyzed biochemical reactions in novel media: A review. Chemical Engineering Communications 153(1): 365-404
Pratush, A., Gupta, A., Vyas, G., & Sharma, P. (2013). Bacterial Lipases: Production Strategies and Industrial Applications. Microbiology Application. 64-83
Treves, C., M.T. Vincenzini, F., Favilli, P., Vanni, V. & Baccari, (1984). On the interaction between synthetic detergents and enzymatic proteins. Canadian J. Biochem. Cell Biol., 62: 55-59.
Vulfson, E.N. (1994). Industrial applications of lipases in Lipases, ed. by Wooley P. and Petersen S.B., Cambridge, Great Britain: Cambridge University Press; p. 271.
Wiseman, A. (1995) Introduction to Principles. In: Wiseman, A., Ed., Hand Book of Enzyme Biotechnology, 3rd Edition, Ellis Horwood Ltd. T.J. Press Ltd. Padstow, 3-8.
License
Copyright (c) 2021 Prapti Sedijani, Dewa Ayu Citra Rasmi, Kusmiyati Kusmiyati, Riska Agustina Anggriani
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
