Genetic Variability of Galoba Durian (Amomum spp) Center Mollucas and North Halmahera Based on RAPD
DOI:
10.29303/jppipa.v8i2.1353Published:
2022-04-30Issue:
Vol. 8 No. 2 (2022): AprilKeywords:
Genetic variation, Galoba Durian, RAPDResearch Articles
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Abstract
Galoba Durian is one of the endemic flora of Maluku, which is used as a medicinal plant and essential oil with red and green fruit colors. This study aimed to analyze the genetic variation of Galoba Durian in Central Maluku and North Halmahera based on the molecular marker of RAPD. This study began with a survey of sampling, isolation, and amplification of DNA using five RAPD primers. The results showed that all DNA bands were polymorphic as many as 67 with genetic similarity ranging from 20% - 31%, which grouped four samples into two groups. The first group is red Galoba Durian from North Halmahera (S1), red (S3) and green (S4) from Central Maluku, while the second group is green Galoba Durian from North Halmahera (S2).
References
Abady, S., Shimelis, H., Janila, P., Yaduru, S., Shayanowako, A. I. T., Deshmukh, D., Chaudhari, S., & Manohar, S. S. (2021). Assessment of the genetic diversity and population structure of groundnut germplasm collections using phenotypic traits and SNP markers: Implications for drought tolerance breeding. PLoS ONE, 16(11 November), 1–25. https://doi.org/10.1371/journal.pone.0259883
Bolibok-Bragoszewska, H., Targońska, M., Bolibok, L., Kilian, A., & Rakoczy-Trojanowska, M. (2014). Genome-wide characterization of genetic diversity and population structure in Secale. BMC Plant Biology, 14(1), 1–15. https://doi.org/10.1186/1471-2229-14-184
Bousba, R., Gueraiche, S., Kanouni, M.R., Bounar, R., Djekoune, A., Khammar, H., Nadia Y. (2020). Genotypic diversity assesment of some durum wheat (Triticum durum) genotypes using RAPD analysis. Biodiversitas 21(6), 2696-2701.https://doi.org/10.13057/biodiv/b210643.
Cheema, S. K., & Pant, M. R. (2013). Karyotype analysis of seven cultivated varieties of Capsicum annuum L. Caryologia, 66(1), 70–75. https://doi.org/10.1080/00087114.2013.787204
Ding, H. B., Yang, B., Maw, M. B., Win, P. P., & Tan, Y. H. (2020). Taxonomic studies on Amomum roxburgh s.l. (Zingiberaceae) in Myanmar II: One new species and five new records for the flora of Myanmar. PhytoKeys, 138, 139–153. https://doi.org/10.3897/phytokeys.138.38736
Ewon, K., & Bhagya, A. S. (2019). A review on golden species of Zingiberaceae family around the world: Genus Curcuma. African Journal of Agricultural Research, 14(9), 519–531. https://doi.org/10.5897/ajar2018.13755
Govindaraj, M., Vetriventhan M., & Srinivasan M. (2015). Importance of genetic diversity assesment in crop plants and its recent advances: an overview of its analytical perspectives. Genetic Research International, 1-14. http://dx.doi.org/10.1155/2015/431487
Huang, S. (2016). New thoughts on an old riddle: What determines genetic diversity within and between species? Genomics, 108(1), 3–10. https://doi.org/10.1016/j.ygeno.2016.01.008
John K, W., Prince, L. M., & Williams, K. J. (2002). The phylogeny and a new classification of the gingers (Zingiberaceae): Evidence from molecular data. American Journal of Botany, 89(10), 1682–1696. https://doi.org/10.3732/ajb.89.10.1682
Li, H., Matthew C, Donglin Z. (2020). Assesing genetic diversity and population structure of Kalmia latifolia L. in the eastern United States: An essential step towards breeding for adaptability to southeastern environmental conditions. Sustainability 12:8284. http://doi.org/10.3390/su12198284
Muriira, N.G., Alice M, Anmien Y, Jianchu X., & Aizhong L. (2018). Genetic diversity analysis reveals genetic differentiation and strong population structure in Calotrophis plants. Scientific Reports 8:7832. https://doi.org/10.1038/s41598-018-26275-x
Oktavia, F.. Lasminingsih. M., & Kuswahadi. (2011). Variation in oil contents, and seed and seedling characteristics of Jatropha curcas of West Nusa Tenggara selected genotypes and their first improved population. Nusantara Bioscience, 3(3), 124–129. https://doi.org/10.13057/nusbiosci/n030304
Orozco-Castillo, C., Chalmers, K. J., Waugh, R., & Powell, W. (1994). Detection of genetic diversity and selective gene introgression in coffee using RAPD markers. Theoretical and Applied Genetics: International Journal of Plant Breeding Research, 87(8), 934–940. https://doi.org/10.1007/BF00225787
Pamoengkas, P., Rachmat, H. H., & Khalifa, N. (2020). The growth of shorea leprosula at various planting distances and slopes in gunung dahu research forest, Bogor, Indonesia. Biodiversitas, 21(9), 4396–4404. https://doi.org/10.13057/biodiv/d210959
Peakall, R., & Smouse, P. E. (2012). GenALEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics, 28(19), 2537–2539. https://doi.org/10.1093/bioinformatics/bts460
Probojati, L.T., Wahyudi D., & Hapsari L. (2019). Clustering analysis and genome inference of Pisang Raja Lokal Cultivars (Musa spp.) from Java island by Random Amplified Polymorphic DNA (RAPD) Marker. Journal of Tropical Biodiversity and Biotechnology 4(2), 42-53. https://doi.org/10.22146/jtbb/44047
Saensouk, P., & Saensouk, S. (2021). Diversity, traditional uses and conservation status of zingiberaceae in Udorn Thani Province, Thailand. Biodiversitas, 22(8), 3083–3097. https://doi.org/10.13057/biodiv/d220801
Solin, N. W. N. M., Sobir, & Toruan-Mathius, N. (2014). Genetic diversity of DXP population yield component in oil palm’s paternal half-sib family based on microsatellite markers. Energy Procedia, 47, 196–203. https://doi.org/10.1016/j.egypro.2014.01.214
Wu, Q., Zang, F., Ma, Y., Zheng, Y., & Zang, D. (2020). Analysis of genetic diversity and population structure in endangered Populus wulianensis based on 18 newly developed EST-SSR markers. Global Ecology and Conservation, 24(1), 1–13. https://doi.org/10.1016/j.gecco.2020.e01329
Xia, Y. M., Kress, W. J., & Prince, L. M. (2004). Phylogenetic analyses of Amomum (Alpinioideae: Zingiberaceae) using ITS and matK DNA sequence data. Systematic Botany, 29(2), 334–344. https://doi.org/10.1600/036364404774195520
Author Biographies
Katerina Jagagura Peilouw, University of Pattimura
Adriana Hiariej, University of Pattimura
Anneke Pesik, Universitas Pattimura
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Copyright (c) 2022 Katerina Jagagura Peilouw, Adriana Hiariej, Anneke Pesik
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