Genetic Variability of Galoba Durian (Amomum spp) Center Mollucas and North Halmahera Based on RAPD

Authors

Katerina Jagagura Peilouw , Adriana Hiariej , Anneke Pesik

DOI:

10.29303/jppipa.v8i2.1353

Published:

2022-04-30

Issue:

Vol. 8 No. 2 (2022): April

Keywords:

Genetic variation, Galoba Durian, RAPD

Research Articles

Downloads

How to Cite

Peilouw, K. J. ., Hiariej, A. ., & Pesik, A. (2022). Genetic Variability of Galoba Durian (Amomum spp) Center Mollucas and North Halmahera Based on RAPD. Jurnal Penelitian Pendidikan IPA, 8(2), 579–583. https://doi.org/10.29303/jppipa.v8i2.1353

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

Galoba Durian is one of the endemic flora of Maluku, which is used as a medicinal plant and essential oil with red and green fruit colors. This study aimed to analyze the genetic variation of Galoba Durian in Central Maluku and North Halmahera based on the molecular marker of RAPD. This study began with a survey of sampling, isolation, and amplification of DNA using five RAPD primers. The results showed that all DNA bands were polymorphic as many as 67 with genetic similarity ranging from 20% - 31%, which grouped four samples into two groups. The first group is red Galoba Durian from North Halmahera (S1), red (S3) and green (S4) from Central Maluku, while the second group is green Galoba Durian from North Halmahera (S2).

References

Abady, S., Shimelis, H., Janila, P., Yaduru, S., Shayanowako, A. I. T., Deshmukh, D., Chaudhari, S., & Manohar, S. S. (2021). Assessment of the genetic diversity and population structure of groundnut germplasm collections using phenotypic traits and SNP markers: Implications for drought tolerance breeding. PLoS ONE, 16(11 November), 1–25. https://doi.org/10.1371/journal.pone.0259883

Bolibok-Bragoszewska, H., Targońska, M., Bolibok, L., Kilian, A., & Rakoczy-Trojanowska, M. (2014). Genome-wide characterization of genetic diversity and population structure in Secale. BMC Plant Biology, 14(1), 1–15. https://doi.org/10.1186/1471-2229-14-184

Bousba, R., Gueraiche, S., Kanouni, M.R., Bounar, R., Djekoune, A., Khammar, H., Nadia Y. (2020). Genotypic diversity assesment of some durum wheat (Triticum durum) genotypes using RAPD analysis. Biodiversitas 21(6), 2696-2701.https://doi.org/10.13057/biodiv/b210643.

Cheema, S. K., & Pant, M. R. (2013). Karyotype analysis of seven cultivated varieties of Capsicum annuum L. Caryologia, 66(1), 70–75. https://doi.org/10.1080/00087114.2013.787204

Ding, H. B., Yang, B., Maw, M. B., Win, P. P., & Tan, Y. H. (2020). Taxonomic studies on Amomum roxburgh s.l. (Zingiberaceae) in Myanmar II: One new species and five new records for the flora of Myanmar. PhytoKeys, 138, 139–153. https://doi.org/10.3897/phytokeys.138.38736

Ewon, K., & Bhagya, A. S. (2019). A review on golden species of Zingiberaceae family around the world: Genus Curcuma. African Journal of Agricultural Research, 14(9), 519–531. https://doi.org/10.5897/ajar2018.13755

Govindaraj, M., Vetriventhan M., & Srinivasan M. (2015). Importance of genetic diversity assesment in crop plants and its recent advances: an overview of its analytical perspectives. Genetic Research International, 1-14. http://dx.doi.org/10.1155/2015/431487

Huang, S. (2016). New thoughts on an old riddle: What determines genetic diversity within and between species? Genomics, 108(1), 3–10. https://doi.org/10.1016/j.ygeno.2016.01.008

John K, W., Prince, L. M., & Williams, K. J. (2002). The phylogeny and a new classification of the gingers (Zingiberaceae): Evidence from molecular data. American Journal of Botany, 89(10), 1682–1696. https://doi.org/10.3732/ajb.89.10.1682

Li, H., Matthew C, Donglin Z. (2020). Assesing genetic diversity and population structure of Kalmia latifolia L. in the eastern United States: An essential step towards breeding for adaptability to southeastern environmental conditions. Sustainability 12:8284. http://doi.org/10.3390/su12198284

Muriira, N.G., Alice M, Anmien Y, Jianchu X., & Aizhong L. (2018). Genetic diversity analysis reveals genetic differentiation and strong population structure in Calotrophis plants. Scientific Reports 8:7832. https://doi.org/10.1038/s41598-018-26275-x

Oktavia, F.. Lasminingsih. M., & Kuswahadi. (2011). Variation in oil contents, and seed and seedling characteristics of Jatropha curcas of West Nusa Tenggara selected genotypes and their first improved population. Nusantara Bioscience, 3(3), 124–129. https://doi.org/10.13057/nusbiosci/n030304

Orozco-Castillo, C., Chalmers, K. J., Waugh, R., & Powell, W. (1994). Detection of genetic diversity and selective gene introgression in coffee using RAPD markers. Theoretical and Applied Genetics: International Journal of Plant Breeding Research, 87(8), 934–940. https://doi.org/10.1007/BF00225787

Pamoengkas, P., Rachmat, H. H., & Khalifa, N. (2020). The growth of shorea leprosula at various planting distances and slopes in gunung dahu research forest, Bogor, Indonesia. Biodiversitas, 21(9), 4396–4404. https://doi.org/10.13057/biodiv/d210959

Peakall, R., & Smouse, P. E. (2012). GenALEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics, 28(19), 2537–2539. https://doi.org/10.1093/bioinformatics/bts460

Probojati, L.T., Wahyudi D., & Hapsari L. (2019). Clustering analysis and genome inference of Pisang Raja Lokal Cultivars (Musa spp.) from Java island by Random Amplified Polymorphic DNA (RAPD) Marker. Journal of Tropical Biodiversity and Biotechnology 4(2), 42-53. https://doi.org/10.22146/jtbb/44047

Saensouk, P., & Saensouk, S. (2021). Diversity, traditional uses and conservation status of zingiberaceae in Udorn Thani Province, Thailand. Biodiversitas, 22(8), 3083–3097. https://doi.org/10.13057/biodiv/d220801

Solin, N. W. N. M., Sobir, & Toruan-Mathius, N. (2014). Genetic diversity of DXP population yield component in oil palm’s paternal half-sib family based on microsatellite markers. Energy Procedia, 47, 196–203. https://doi.org/10.1016/j.egypro.2014.01.214

Wu, Q., Zang, F., Ma, Y., Zheng, Y., & Zang, D. (2020). Analysis of genetic diversity and population structure in endangered Populus wulianensis based on 18 newly developed EST-SSR markers. Global Ecology and Conservation, 24(1), 1–13. https://doi.org/10.1016/j.gecco.2020.e01329

Xia, Y. M., Kress, W. J., & Prince, L. M. (2004). Phylogenetic analyses of Amomum (Alpinioideae: Zingiberaceae) using ITS and matK DNA sequence data. Systematic Botany, 29(2), 334–344. https://doi.org/10.1600/036364404774195520

Author Biographies

Katerina Jagagura Peilouw, University of Pattimura

Adriana Hiariej, University of Pattimura

Anneke Pesik, Universitas Pattimura

License

Copyright (c) 2022 Katerina Jagagura Peilouw, Adriana Hiariej, Anneke Pesik

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).