The Efficiency of Heavy Metal Analysis Method in Marine Fish Samples by Atomic Absorption Spectrophotometry

Authors

Mutiah Mutiah , Jackson Siahaan , I Nyoman Loka , Jono Irawan

DOI:

10.29303/jppipa.v8i2.1489

Published:

2022-04-30

Issue:

Vol. 8 No. 2 (2022): April

Keywords:

Heavy Metals, Atomic absorption spectrophotometry, Double standard addition

Research Articles

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How to Cite

Mutiah, M., Siahaan, J. ., Loka, I. N. ., & Irawan, J. . (2022). The Efficiency of Heavy Metal Analysis Method in Marine Fish Samples by Atomic Absorption Spectrophotometry . Jurnal Penelitian Pendidikan IPA, 8(2), 963–968. https://doi.org/10.29303/jppipa.v8i2.1489

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Abstract

This research studies the efficient method for heavy metal analysis in marine fish samples by atomic absorption spectrophotometry. The dissolution of the samples used the wet destruction technique in 2 ways. The first method uses a mixture of HNO3, H2SO4, HClO4 (1:2:1), and the second method uses a mixture of HNO3and H2SO4 (1:1). Tuna (Euthynnus affinis) was taken as Marine fish samples. The results showed that the first method took 180 minutes with a % recovery of 98.79% - 99.55%. In contrast, the second method took 240 minutes and a % recovery of 98.08% - 98.45%. The results of measurements by atomic absorption spectrophotometry (AAS) using standard calibration curve and standard addition techniques are as follows: using standard regression curve technique obtained Pb (0.664 ± 0.067) mg/kg, Cu (2.780 ± 0.01) mg/kg, Cd (0.192 ± 0.044) mg/kg, and Zn (1.824 ± 0.075) mg/kg, while the standard addition method obtained Pb (0.612 ± 0.016) mg/kg, Cu (2.364 ± 0.016) mg/kg, Cd (0.148 ± 0.029) mg/kg and Zn (1.692 ± 0.016) mg/kg. The calculation of the level of accuracy of the analysis showed that the standard addition method RSD gave a precision value of 0.97% - 3.76% and the standard curve method RSD with a precision of 5.76% - 31.77%. In conclusion, (1) Heavy metal content in tuna (Euthynnus affinis) is still within the permissible limits. (2) wet digestion technique using a mixture of HNO3, H2SO4, HClO4 (1:2:1) can be more efficient. (3) The measurement results of the standard addition method give more precise results

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Author Biographies

Mutiah Mutiah, University of Mataram

Jackson Siahaan, University of Mataram

I Nyoman Loka, University of Mataram

Jono Irawan, University of Mataram

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Copyright (c) 2022 Muti'ah Muti'ah, Jackson Siahaan, I Nyoman Loka, Jono Irawan

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