Separation of N-Methylhydroxamic Fatty Acids Based on Ketapang Seed Oil using High Performance Liquid Chromatography

Authors

Ana Restu Nirwana , Dedy Suhendra , Erin Ryantin Gunawan

DOI:

10.29303/jppipa.v8i5.1611

Published:

2022-11-30

Issue:

Vol. 8 No. 5 (2022): November

Keywords:

Lipase, Ketapang oil, N-methylhydroxilamine, N-MFHAs, HPLC

Research Articles

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Nirwana, A. R., Suhendra, D., & Gunawan, E. R. (2022). Separation of N-Methylhydroxamic Fatty Acids Based on Ketapang Seed Oil using High Performance Liquid Chromatography. Jurnal Penelitian Pendidikan IPA, 8(5), 2329–2334. https://doi.org/10.29303/jppipa.v8i5.1611
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Abstract

N-methyl fatty hydroxamic acids (N-MFHAs) is a derivative of a hydroxamic acid (FHA), were synthesized from ketapang seed oil (Terminalia catappa L.) with N-methyl hydroxylamine enzymatically using immobilized lipase (Lipozyme TL IM). N-MFHAs that are synthesized are still in a mixed state of triglycerides of the ketapang seed oil. This research aims to determine the optimum conditions for the separation of N-MFHAs into their single components and their percentage composition using High Performance Liquid Chromatography (HPLC). The specification of the HPLC system includes using the reverse phase column SGE C-18 ODS-2 and UV detector 213 nm. The optimum conditions for the separation of N-MFHAs in the HPLC system include using the mobile phase of methanol 100% and flow rate of 0.25 mL/minutes with an injection volume of 20 µL and sample concentration of 10.000 ppm. The percentage of the N-MFHAs composition successfully separated using HPLC were linoleoyl-methyl-fatty hydroxamic acid (13.09%), oleoyl-methyl-fatty hydroxamic acid (62.46%), palmitoyl-methyl-fatty hydroxamic acid (19.51%), and stearoyl-methyl-fatty hydroxamic acid (4.93%).

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Author Biography

Dedy Suhendra, SCOPUS ID: 12645012500, University of Mataram, Department of Chemistry, Lombok Strait, Indonesia

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Copyright (c) 2022 Dedy Suhendra, Erin Ryantin Gunawan, Ana Restu Nirwana

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