Growth of Arthrospira platensis with Different Nitrogen Sources

Authors

Nur Aliya Nabila Zsalzsabil , Yenny Risjani , Muhamad Firdaus , Karimah

DOI:

10.29303/jppipa.v9i3.2754

Published:

2023-03-31

Issue:

Vol. 9 No. 3 (2023): March

Keywords:

Arthrospira platensis, Growth, Nitrogen source, Water quality

Research Articles

Downloads

How to Cite

Zsalzsabil, N. A. N., Risjani, Y., Firdaus, M., & Karimah, K. (2023). Growth of Arthrospira platensis with Different Nitrogen Sources. Jurnal Penelitian Pendidikan IPA, 9(3), 1523–1529. https://doi.org/10.29303/jppipa.v9i3.2754
Crossref
Scopus
Google Scholar
Europe PMC

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

Microalgae is a promising and innovative biological resource. Indonesia has the potential for the development of microalgae production. One of the microalgae that have high economic value is Arthrospira platensis. In the production of microalgae, the development of suitable media and environmental conditions need to be factors that must be considered. This study aimed to analyze the effect of different nitrogen sources and nitrogen doses and their interaction on the growth of microalgae Arthrospira platensis cultured in fresh water. In addition, analyzing the water quality of Arthrospira platensis culture media. This study used an experimental method of Completely Randomized Factorial Design) with two factors. The first factor is the nitrogen source (Sodium nitrate, Potassium nitrate, and Calcium nitrate). The second factor is the dose of nitrogen source (2 g/l.2.5 g/l.3 g/l). The main parameter observed was microalgae growth, while the supporting parameter was the water quality which affected the growth of Arthrospira platensis. The highest density results were found in the provision of a nitrogen source of Ca(NO3)2 3 g/l, which was 370,000 cells/ml. The results of the ANOVA test showed that the type of nitrogen source, the dose, and the interaction between the two affected the specific growth rate of Arthrospira platensis. The environmental conditions during culture ranged from 20.2°C-26.2°C, pH values ​​8.95 – 10.18, and dissolved oxygen 9.17 – 9.77 mg/L.

References

Addini, I., Saputra, D., Ilhamdy, A. F., & Julianto, T. (2017). Pertumbuhan Mikroalga Spirulina platensis yang dikultur dengan Media Teknis. Jurnal Intek Akuakultur, 1(1), 51–52. https://doi.org/10.31629/intek.v1i1.76

Afriza, Z., Diansyah, G., & Purwiyanto, A. I. S. (2015). Pengaruh Pemberian Pupuk Urea (CH4N2O) dengan Dosis Berbeda Terhadap Kepadatan Sel dan Laju Pertumbuhan Porphyridium sp. pada Kultur Fitoplankton Skala Laboratorium. Maspari Journal, 7(2), 33–40. https://doi.org/10.56064/maspari.v7i2.2439

Alghanmi, H. A., & Jawad, H. M. (2019). Effect of Environmental Factors on Cyanobacteria Richness in Some Agricultural Soils. Geomicrobiology Journal, 36(1), 75–84. https://doi.org/10.1080/01490451.2018.1517196

Arahou, F., Hassikou, R., Arahou, M., Rhazi, L., & Wahby, I. (2021). Influence of Culture Conditions on Arthrospira platensis Growth and Valorization of Biomass as Input for Sustainable Agriculture. Aquaculture International, 29(5), 2009–2020. https://link.springer.com/article/10.1007/s10499-021-00730-5

Arsad, S., Sari., L. A., Suherman., S. P., Cahyani., D., Nadhira., T., Yulianda., E. N., Musa., M., Lusiana, E. D., & Prasetiya, F. S. (2020). Utilization of tofu wastewater Chlorella pyrenoidosa growth medium. AACL Bioflux, 13(5). http://www.bioflux.com.ro/aacl

Brindhadevi, K., Mathimani, T., Rene, E. R., Shanmugam, S., Chi, N. T. L., & Pugazhendhi, A. (2021). Impact of cultivation conditions on the biomass and lipid in microalgae with an emphasis on biodiesel. Fuel, 284, 119058. https://doi.org/10.1016/j.fuel.2020.119058

Buwono, N. R., & Nurhasanah, R. Q. (2018). Studi Pertumbuhan Populasi Spirulina sp. pada Skala Kultur yang Berbeda [Study of Spirulina sp. Population Growth in The Different Culture Scale. Jurnal Ilmiah Perikanan Dan Kelautan, 10(1), 26. https://doi.org/10.20473/jipk.v10i1.8516

Carneiro, M., Cicchi, B., Maia, I. B., Pereira, H., Zittelli, G. C., Varela, J., & Torzillo, G. (2020). Effect of Temperature on Growth, Photosynthesis and Biochemical Composition of Nannochloropsis oceanica, Grown Outdoors in Tubular Photobioreactors. Algal Research, 49, 101923. https://doi.org/10.1016/j.algal.2020.101923.

Fradinho, P., Niccolai, A., Soares, R., Rodolfi, L., Biondi, N., Tredici, M. R., Sousa, I., & Raymundo, A. (2020). Effect of Arthrospira platensis (spirulina) incorporation on the rheological and bioactive properties of gluten-free fresh pasta. Algal Research, 45, 101743. https://doi.org/10.1016/j.algal.2019.101743

Ilhami, B. T. K., Japa, L., Astuti, S. P., & Kurnianingsih, R. (2015). Pengaruh Perbedaan Umur Panen Terhadap Kandungan Lemak Nitzschia sp. Jurnal Biologi Tropis, 15(2). https://doi.org/10.29303/jbt.v15i2.203

Jesus, C. S., Silva Uebel, L., Costa, S. S., Miranda, A. L., Morais, E. G., Morais, M. G., & Druzian, J. I. (2018). Outdoor Pilot-Scale Cultivation of Spirulina sp. LEB-18 in Different Geographic Locations for Evaluating Its Growth and Chemical Composition. Bioresource Technology, 256, 86–94. https://doi.org/10.1016/j.biortech.2018.01.149.

Juneja, A., Ceballos, R. M., & Murthy, G. S. (2013). Effects of Environmental Factors and Nutrient Availability on The Biochemical Composition of Algae for Biofuels Production: a review. Energies, 6(9), 4607–4638. https://doi.org/10.3390/en6094607.

Jung, F., Krüger-Genge, A., Waldeck, P., & Küpper, J.-H. (2019). Spirulina platensis, a super food? Journal of Cellular Biotechnology, 5(1), 43–54. https://doi.org/10.3233/JCB-189012

Lesmana, P. A., Diniarti., N., & Setyo, B. D. H. (2019). Pengaruh Penggunaan Limbah Air Budidaya Ikan Lele sebagai Media Pertumbuhan Spirulina sp. Jurnal Perikanan, 9(1), 50–65. https://doi.org/10.29303/jp.v9i1.136.

Li, G., Bai, X., Li, H., Lu, Z., Zhou, Y., Wang, Y., & Huang, Z. (2019). Nutrients Removal and Biomass Production from Anaerobic Digested Effluent by Microalgae: A review. International Journal of Agricultural and Biological Engineering, 12(5), 8–13. https://doi.org/10.25165/j.ijabe.20191205.3630.

Moheimani, N. R., Borowitzka, M. A., Isdepsky, A., & Sing, S. (2013). Standard Methods for Measuring Growth of Algae and Their Composition. In Algae for biofuels and energy. Springer.

Morales, M., Sánchez, L., & Revah, S. (2018). The Impact of Environmental Factors on Carbon Dioxide Fixation by Microalgae. FEMS Microbiology Letters, 365(3). https://doi.org/10.1093/femsle/fnx262.

Muliani, M., Ayuzar, E., & Amri, M. C. (2018). Pengaruh Pemberian Pupuk Kascing (Bekas Cacing) yang Difermentasi dengan Dosis yang Berbeda dalam Kultur Spirulina sp. Acta Aquatica: Aquatic Sciences Journal, 5(1), 30–35. https://doi.org/10.29103/aa.v5i1.658.

Mutia, S., Nedi, S., & Elizal, E. (2021). Effect of Nitrate and Phospate Concentration on Spirulina platensis with Indoor Scale. Asian Journal of Aquatic Sciences, 4(1), 29–35. https://doi.org/10.31258/ajoas.4.1.29-35

Nur, M. M. A. (2014). Potency of Microalgae as Source of Functional Food in Indonesia (Overview). Eksergi, 11(2), 1. https://doi.org/10.31315/e.v11i2.363

Oktaviani, D., Adisyahputra, & Amelia, N. (2017). Pengaruh Kadar Nitrat terhadap Pertumbuhan dan Kadar Lipid Mikroalga Melosira sp. sebagai Tahap Awal Produksi Biofuel. Jurnal Risenologi KPM UNJ, 2(1), 1–13. https://doi.org/10.47028/j.risenologi.2017.21.3.

Piu, N. J. F., Koniyo, Y., & Salam, A. (2018). MS The Effect of Salinity and Light on the Density of Spirulina Platensis , by using Walne Media. European Multidiciplinary Journal of Modern Science, 1–16. https://emjms.academicjournal.io

Prasadi, O. (2018). Pertumbuhan dan Biomasa Spirulina sp. dalam Media Pupuk sebagai Bahan Pangan Fungsional
[Growth and Biomass of Spirulina sp. in Fertilizer Ingredients as Functional Food Ingredients]. Jurnal Ilmiah Perikanan Dan Kelautan, 10(2), 119–123. https://doi.org/10.20473/jipk.v10i2.10497

Rahayu, S. M., Damar, A., & Krisanti, M. (2022). Perbedaan Konsentrasi Nitrat dan Intensitas Cahaya terhadap Laju Pertumbuhan diatom Chaetoceros muelleri. Acta Aquatica: Aquatic Sciences Journal, 9(2), 95–100. https://doi.org/10.29103/aa.v9i2.8126.

Risjani, Y., Witkowski, A., Kryk, A., Yunianta, Górecka, E., Krzywda, M., Safitri, I., Sapar, A., DÄ…bek, P., Arsad, S., Gusev, E., Rudiyansyah, Peszek, Å., & Wróbel, R. J. (2021). Indonesian coral reef habitats reveal exceptionally high species richness and biodiversity of diatom assemblages. Estuarine, Coastal and Shelf Science, 261(June), 107551. https://doi.org/10.1016/j.ecss.2021.107551

Serra-Maia, R., Bernard, O., Goncalves, A., Bensalem, S., & Lopes, F. (2016). Influence of Temperature on Chlorella vulgaris Growth and Mortality Rates in a Photobioreactor. Algal Research, 18, 352–359. https://doi.org/10.1016/j.algal.2016.06.016.

Soni, R. A., Sudhakar, K., & Rana, R. S. (2019). Comparative study on the growth performance of Spirulina platensis on modifying culture media. Energy Reports, 5, 327–336. https://doi.org/10.1016/j.egyr.2019.02.009

Suharyanto, T. P., Permatasari, S., & Syamsu, K. (2014). Produksi Spirulina platensis dalam Fotobioreaktor Kontinyu menggunakan media Limbah Cair Pabrik Kelapa Sawit. Menara Perkebunan, 82(1), 1–9. https://doi.org/10.22302/iribb.jur.mp.v82il.25.

Suyoso, A. L. A., Sari, L. A., Sari, P. D. W., & Nindarwi, D. D. (2022). Evaluation of The Culture of Spirulina sp. with Walne Nutrient Plus Vitamin B12, KCl, NPK, ZA CaO and urea. IOP Conference Series, 1036(1), 12026. https://doi.org/10.1088/1755-1315/1036/1/012026.

Tangguda, S., & Prasetia, I. N. D. (2019). Produksi Chlorella sp. dengan Perlakuan Limbah Cair Tambak Udang Vaname (Litopenaeus vannamei) Steril (Production of Chlorella sp. with Steril White Shrimp (Litopenaeus vannamei) Liquid Waste Treatment). Saintek Perikanan: Indonesian Journal of Fisheries Science and Technology, 14(2), 96. https://doi.org/10.14710/ijfst.14.2.96-99

Ukhty, N. (2018). Komponen Metabolit Sekunder dan Aktivitas Antioksidan Spirulina fusiformis yang Dikultur pada Media Campuran (Pupuk R1, Urea dan Katalis. Jurnal Perikanan Tropis, 5(2), 161–168. https://doi.org/10.35308/jpt.v5i2.1035.

Usharani, G., Saranraj, P., & Kanchana, D. (2012). Spirulina Cultivation: A review. Spirulina Cultivation: A ReviewInt J Pharm Biol Arch, 3(6), 1327–1341. https://rb.gy/17hs

Veza, I., Said, M. F. M., Abas, M. A., Latiff, Z. A., Perang, M. R. M., & Djamari, D. W. (2021). Future Direction of Microalgae Biodiesel in Indonesia. Journal of Advanced Research in Applied Sciences and Engineering Technology, 25(1), 1–6. https://doi.org/10.37934/araset.25.1.16.

Viqram, V., Abidin, Z., & Mukhlis, A. (2018). Pengaruh Penambahan Pupuk Organik Guano dengan Konsentrasi yang Berbeda terhadap Laju Pertumbuhan Spirulina sp. Jurnal Perikanan Unram, 8(2), 58–65. https://doi.org/10.29303/jp.v8i2.119.

Ye, C., Mu, D., Horowitz, N., Xue, Z., Chen, J., Xue, M., Zhou, Y., Klutts, M., & Zhou, W. (2018). Life cycle assessment of industrial scale production of spirulina tablets. Algal Research, 34, 154–163. https://doi.org/10.1016/j.algal.2018.07.013

Yousuf, A. (2020). Fundamentals of Microalgae Cultivation. In Microalgae cultivation for biofuels production. Academic Press.

Author Biographies

Nur Aliya Nabila Zsalzsabil, Faculty of Fisheries and Marine Science, University of Brawijaya, Malang

Yenny Risjani, Department of Fisheries and Marine Resources Management, Faculty of Fisheries and Marine Science, University Brawijaya, Malang

Muhamad Firdaus, Department of Fisheries and Marine Resources Management, Faculty of Fisheries and Marine Science, University Brawijaya, Malang

Karimah, Brawijaya University

License

Copyright (c) 2023 Nur Aliya Nabila Zsalzsabil, Yenny Risjani, Muhamad Firdaus

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).