Biomass Production of Subang Isolated Scenedesmus in Tube-shaped Photobioreactor with the Exposure of Audible Sound (Music): Photobioreactor Improvement Strategy
DOI:
10.29303/jppipa.v9i3.2765Published:
2023-03-31Issue:
Vol. 9 No. 3 (2023): MarchKeywords:
Audible sound, Cell density, Growth, Music, Photobioreactor (PBR), ScenedesmusResearch Articles
Downloads
How to Cite
Downloads
Metrics
Abstract
The cultivation of Subang Isolated Scenedesmus in tube-shaped photobioreactors (PBRs) with the exposure of audible sound (music) was done. The study was done to know the effect of different music to the growth and culture appearance of Subang Isolated Scenedesmus. Subang Isolated Scenedesmus itself is Indonesia’s indigenous microalgae from Subang, West Java. This study was using three groups of Subang Isolated Scenedesmus PBRs. One group was control group and not exposed to any sound (Control-PBR), one group was exposed to music called “Blues for Elle†(PBR-A), and another group was exposed to music called “Bloom†(PBR-B). Based on 8 days of observation, it is known that Subang isolated Scenedesmus in PBR-A has the highest average cell density at the peak phase (1.81±0.16 x 105 cells/mL), highest growth rate (26.22 % per day), and most intense green color compared to Subang isolated Scenedesmus in PBR-B and Control-PBR. Related-Samples Friedman’s Two-Way ANOVA by Ranks test has proven that the average cell density value of Subang isolated Scenedesmus in Control-PBR, PBR-A, and PBR-B are not the same (α = 95%, sig = 0.013). Therefore, different audible sound (music) with certain intensity and frequency may affect the growth of Subang Isolated Scenedesmus differently
References
Barsanti, L., & Gualtieri, P. (2023). Algae: Anatomy, Biochemistry, and Biotechnology (3rd ed.). CRC Press.
Begum, H., Yusoff, F. M. D., Banerjee, S., Khatoon, H., & Shariff, M. (2016). Availability and Utilization of Pigments from Microalgae. Critical Reviews in Food Science and Nutrition, 56(13), 2209–2222. https://doi.org/10.1080/10408398.2013.764841
Bellinger, E. G., & Sigee, D. C. (2015). Freshwater Algae: Identification, Enumeration, and Use as Bioindicators. John Wiley & Sons.
Christwardana, M., & Hadiyanto, H. (2017). The Effects of Audible Sound for Enhancing the Growth Rate of Microalgae Haematococcus pluvialis in Vegetative Stage. HAYATI Journal of Biosciences, 24(3), 149–155. https://doi.org/10.1016/j.hjb.2017.08.009
Difusa, A., Talukdar, J., Kalita, M. C., Mohanty, K., & Goud, V. V. (2015). Effect of light intensity and pH condition on the growth, biomass and lipid content of microalgae Scenedesmus species. Biofuels, 6(1–2), 37–44. https://doi.org/10.1080/17597269.2015.1045274
Draaisma, R. B., Wijffels, R. H., Slegers, P. M., Brentner, L. B., Roy, A., & Barbosa, M. J. (2013). Food commodities from microalgae. Current Opinion in Biotechnology, 24(2), 169–177. https://doi.org/10.1016/j.copbio.2012.09.012
Francois, K., Devlieghere, F., Standaert, A. R., Geeraerd, A. H., Cools, I., Van Impe, J. F., & Debevere, J. (2005). Environmental factors influencing the relationship between optical density and cell count for Listeria monocytogenes. Journal of Applied Microbiology, 99(6), 1503–1515. https://doi.org/10.1111/j.1365-2672.2005.02727.x
Huang, Q., Jiang, F., Wang, L., & Yang, C. (2017). Design of Photobioreactors for Mass Cultivation of Photosynthetic Organisms. Engineering, 3(3), 318–329. https://doi.org/10.1016/J.ENG.2017.03.020
Larsen, P., & Gilbert, J. (2013). Microbial Bebop: Creating Music from Complex Dynamics in Microbial Ecology. PLoS ONE, 8(3), 58119. https://doi.org/10.1371/journal.pone.0058119
Lee, Y.-K., Chen, W., Shen, H., Han, D., Li, Y., Jones, H. D. T., Timlin, J. A., & Hu, Q. (2013). Basic Culturing and Analytical Measurement Techniques in Richmond, A. In Q. Hu (Ed.), Handbook of Microalgal Culture (pp. 37–68). John Wiley & Sons, Ltd.
Milano, J., Ong, H. C., Masjuki, H. H., Chong, W. T., Lam, M. K., Loh, P. K., & Vellayan, V. (2016). Microalgae biofuels as an alternative to fossil fuel for power generation. Renewable and Sustainable Energy Reviews, 58, 180–197. https://doi.org/10.1016/j.rser.2015.12.150
Mishra, R. C., Ghosh, R., & Bae, H. (2016). Plant acoustics: in the search of a sound mechanism for sound signaling in plants. Journal of Experimental Botany, 67(15), 4483–4494. https://doi.org/10.1093/jxb/erw235
Prihantini, N. B., Hendrayanti, D., Widyawan, A., & Yuniati, R. (2007). Pemanfaatan Medium Ekstrak Tauge ( MET ) untuk Menghasilkan Biomassa Chlorella sp . dan Scenedesmus sp . dengan Kandungan Protein dan Klorofil. Sains Indonesia, 12(June 2021), 11–24. https://rb.gy/zbixbe
Prihantini, N. B., Rakhmayanti, N., Handayani, S., Sjamsuridzal, W., Wardhana, W., & Nasruddin. (2020). Biomass production of indonesian indigenous leptolyngbya strain on npk fertilizer medium and its potential as a source of biofuel. Evergreen, 7(4), 593–601. https://doi.org/10.5109/4150512
Rastogi, R. P., Pandey, A., Larroche, C., & Madamwar, D. (2018). Algal Green Energy—R&D and Technological Perspectives for Biodiesel Production. Renewable and Sustainable Energy Reviews, 82, 2946–2969. https://doi.org/10.1016/j.rser.2017.10.038
Reguera, G. (2011). When microbial conversations get physical. Trends Microbiol, 19(3), 105–113. https://doi.org/10.1016/j.tim.2010.12.007
Santoso, C. A., Takarina, N. D., Ambarsari, H., Prihantini, N. B., & Sitaresmi, S. (2020). Effect of Hydrocarbon-Polluted Seawater on the Cell Density of Microalgae Scenedesmus vacuolatus Shihira & Krauss. Microbiology Indonesia, 14(3), 108–116. https://doi.org/10.5454/mi.14.3.4
Serway, R. a., & Jewett, J. W. (2012). Physics for Scientists and Engineers with Modern Physic, 7 ed. In Brooks/cole.
Skjånes, K., Rebours, C., & Lindblad, P. (2013). Potential for green microalgae to produce hydrogen, pharmaceuticals and other high value products in a combined process. Critical Reviews in Biotechnology, 33(2), 172–215. https://doi.org/10.3109/07388551.2012.681625
Slade, R., & Bauen, A. (2013). Micro-algae cultivation for biofuels: Cost, energy balance, environmental impacts and future prospects. Biomass and Bioenergy, 53, 29–38. https://doi.org/10.1016/j.biombioe.2012.12.019
Tambunan, R. M. N., Santoso, Y. A., Soekirno, S., Nasruddin, & Prihantini, N. B. (2020). Cultivation of Chlorella DPK-01 using audible sound (music) as a potential strategy for improving photobioreactor system. AIP Conference Proceedings, 2255. https://doi.org/10.1063/5.0013759
Tambunan, R. M. N., Santoso, Y. A., Soekirno, S., Nasruddin, & Prihantini, N. B. (2021). Difference in Time of Audible Sound Exposure to Chlorella DPK-01 in Tubular Photobioreactors: A Strategy to Improve Photobioreactor System. Journal of Advanced Research in Fluid Mechanics and Thermal Sciences, 81(2), 82–88. https://doi.org/10.37934/arfmts.81.2.8288
Walpole, R. E., Myers, R. H., Myers, S. L., & Ye, K. (2012). Probability & Statistics for Engineers & Scientists, 9th ed (9th ed.). Boston: Prentice Hall.
Zhu, Z., Jiang, J., & Fa, Y. (2020). Overcoming the Biological Contamination in Microalgae and Cyanobacteria Mass Cultivations for Photosynthetic Biofuel Production. Molecules, 25(22), 5220. https://doi.org/10.3390/MOLECULES25225220
Author Biography
Nining Betawati Prihantini, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Kampus UI, Depok, 16424, Indonesia
License
Copyright (c) 2023 Rubiantin Mesha Nauli Tambunan, Nining Betawati Prihantini
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).