The Effect of Combination of Oleander Leaf Extract (Nerium oleander L.) With Neem Leaf (Azadiracta indica A. Juss) on Mortality Golden Snail (Pomaceae canaliculata L.)


Supriatno , Nur Asiah , Asiah M D , Djufri , Safrida






Vol. 9 No. 7 (2023): July


Mortality, Neem, Oleander, The golden snail, Toxicity

Research Articles


How to Cite

Supriatno, S., Asiah, N., Asiah M D, A. ., Djufri, D., & Safrida, S. (2023). The Effect of Combination of Oleander Leaf Extract (Nerium oleander L.) With Neem Leaf (Azadiracta indica A. Juss) on Mortality Golden Snail (Pomaceae canaliculata L.) . Jurnal Penelitian Pendidikan IPA, 9(7), 5304–5309.


Download data is not yet available.


Metrics Loading ...


The golden snail is a pest that causes crop failure in rice plants. Farmers control the golden snail by using pesticides, which can have a negative impact on the environment, so another alternative is needed that can control the golden snail, namely vegetable pesticides. This study aims to determine the effect of a combination of oleander leaf extract and neem leaves on the mortality of Keong Mas. The method used in this research is the experimental method with a factorial completely randomised design (CRD). The study consisted of 2 factors, namely factor N (Oleander leaf) and factor A (Azadiracta indica leaf), each of which had 4 levels, so there were 16 treatment combinations and 3 replications. There were 480 golden snails in the study. After 12 hours of observation, the N3A3 treatment tested by ANOVA showed significantly different results (fcount > ftable = 66.00 > 2.01) and caused 30 test animals to die with a mortality rate of 77%.  The conclusion from this study is that the combination of oleander leaf extract with neem leaves greatly influences the mortality of the golden snail


Ahmed, O. H., Ezghayer, M. A., Jabir, M. S., & Tawfeeq, M. F. (2023). Cytotoxic Activity Of Methanolic Extract Of Nerium Oleander Naturally Grown In Iraq. Journal of Survey in Fisheries Sciences, 10, 2517–2524. Retrieved from

Arsi, A., Jarda, E. P., Hamidson, H., Pujiastuti, Y., Gunawan, B., Pratama, R., & Umayah, A. (2022). Laying eggs of Pomacea canaliculata L. on Oryza sativa L. in various ways of plant cultivation in Village Tulus Ayu, Sub District Belitang. Jurnal Lahan Suboptimal: Journal of Suboptimal Lands, 11(2), 179–186.

Braga, T. M., Rocha, L., Chung, T. Y., Oliveira, R. F., Pinho, C., Oliveira, A. I., Morgado, J., & Cruz, A. (2021). Azadirachta Indica A. Juss. In Vivo Toxicity-An Updated Review. Molecules, 26(2), 1–21.

Cesa, S., Sisto, F., Zengin, G., Scaccabarozzi, D., Kokolakis, A. K., Scaltrito, M. M., Grande, R., Locatelli, M., Cacciagrano, F., Angiolella, L., Campestre, C., Granese, A., Chimenti, P., & Basilico, N. (2019). Phytochemical Analyses And Pharmacological Screening Of Neem Oil. South African Journal Of Botany, 120, 331–337.

Dey, B. K., Durgaprasad, K., & Deka, H. (2022). Nerium Oleander:Phytochemistry, Poisoning And Case Studies. International Journal of Biology, Pharmacy and Allied Sciences(IJBPAS), 11(7), 3463-3476.

Dono, D., Hidayat, Y., Suganda, T., Hidayat, S., & Widayani, N. S. (2020). The Toxicity Of Neem (Azadirachta Indica), Citronella (Cymbopogon Nardus), Castor (Ricinus Communis), And Clove (Syzygium Aromaticum) Oil Against Spodoptera Frugiperda. CROPSAVER-Journal of Plant Protection, 3(1), 22–30.

Dwi, R., Windriyati, H., Tikafebianti, L., Anggraeni, G., Agroteknologi, S., Nahdlatul, U., & Purwokerto, U. (2020). Pembuatan Pestisida Nabati Pada Kelompok Tani Wanita Sejahtera Di Desa Sikapat. Dinamisia: Jurnal Pengabdian Kepada Masyarakat, 4(4), 635–642.

Farkhondeh, T., Kianmehr, M., Kazemi, T., & Samarghandian, S. (2020). Toxicity Effects Of Nerium Oleander, Basic And Clinical Evidence : A Comprehensive Review. Human & experimental toxicology, 39(6), 773-784.

Kala, S., Naik, S. N., Patanjali, P. K., & Sogan, N. (2019). Neem Oil Water Dispersible Tablet As Effective Larvicide, Ovicide And Oviposition Deterrent Against Anopheles Culicifacies. South African Journal Of Botany, 123, 387–392.

Maruni, M., Lamangantjo, C. J., & Abdul, A. (2022). Toxicity Test Of Jatropha Leaf Filtrate (Jatropha Curcas L.) On Mortality Of Golden Snail (Pomacea Canaliculata). Inornatus: Biology Education Journal, 2(2), 69–77.

Nasir, B. H., Made, U., Lasmini, S. A., Hayati, N., & Fuqra, I. (2021). Diseminasi Teknologi Pembuatan Pestisida Botani Nerium Oleander Linn. Untuk Pengendalian Hama Tanaman Sayuran. Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR), 4, 262–268.

Osborne, C., Nankishore, A., & Sambhu, H. (2022). Challenges In Rice (Oryza Sativa) Farming : Towards A Biological Control Of The Golden Apple Snail (Pomacea Sp .) In Guyana, South America. International Journal of Multidisciplinary Research and Growth Evaluation, 3(3), 45–49. Retrieved from

Patil, S. P., Chaudhari, R. Y., & Nemade, M. S. (2022). Azadirachta Indica Leaves Mediated Green Synthesis Of Metal Oxide Nanoparticles : A Review. Talanta Open, 5, 100083.

Putri, A. P., Manaf, S., & Salim, M. (2019). Uji Efektivitas Ekstrak Daun Oleander (Nerium Oleander L.) Sebagai Biolarvasida Terhadap Aedes Aegypti. Spirakel, 11(2), 44–52.

Rifda, & Lisdiana, L. (2022). Efektivitas Kombinasi Ekstrak Etanol Daun Kersen Dan Kunyit Sebagai Antibakteri Propionibacterium Acnes. LenteraBio: Berkala Ilmiah Biologi, 11(3), 586-593. Retrieved from

Salim, H. A., Hadi, M., Alsaady, M., & Shiblawi, L. M. (2020). The First Record To Moth Of Ocnogyna Loewii Zell. (Arctiidae: Lepidoptera) On Wheat Plants In Iraq And Evaluate Efficacy Of Some Aqueous Plant Extracts Against Its Caterpillars. Plant Archives, 20(1), 1366–1370. Retrieved from

Sayed, S., Elarnaouty, S., & Ali, E. (2021). Suitability Of Five Plant Species Extracts For Their Compatibility With Indigenous Beauveria Bassiana Against Aphis Gossypii Glov. (Hemiptera : Aphididae). Egyptian Journal of Biological Pest Control, 31(1), 1-8.

Shridhar, N. B. (2022). Nerium Oleander Toxicity : A Review. Int. J. Adv. Acad. Stud., 4(3), 23–32.

Souto, A. L., Sylvestre, M., Tölke, E. D., Tavares, J. F., Barbosa-Filho, J. M., & Cebrián-Torrejón, G. (2021). Plant-Derived Pesticides As An Alternative To Pest Management And Sustainable Agricultural Production: Prospects, Applications And Challenges. Molecules, 26(16).

Subakti Putri, S. N., Susanto, A., Bari, I. N., & Santriyani, A. S. (2022). Pengaruh Beberapa Pelarut Ekstrak Daun Nerium Oleander L. Terhadap Mortalitas, Konsumsi Makan Dan Kelulushidupan Spodoptera Frugiperda J.E. Smith. Agrikultura, 33(3), 369.

Sykes, C. A., Uzal, F. A., Mete, A., Ochoa, J., Filigenzi, M., Poppenga, R. H., & Asin, J. (2022). Renal Lesions In Horses With Oleander (Nerium Oleander) Poisoning. Animals, 12(11), 1443.

Thiyagu, T. T., & Rajeswari, N. (2021). Bioactivity Of Neem Seed Oil Mixed With Pyroligneous Acid From Rice Husks Against Spodoptera Litura Bioactivity Of Neem Seed Oil Mixed With Pyroligneous Acid From Rice Husks Against Spodoptera Litura. In IOP Conference Series: Earth and Environmental Science, 913(1), 012075.

Tripathy, B., Dash, L., & Rout, S. (2022). Pesticide Residues In Vegetables : A Vicious Trend To Break. International Journal of Mechanical Engineering, 7, 260-270. Retrieved from

Wulansari, R., Hidayat, Y., & Dono, D. (2022). Aktivitas Insektisida Campuran Minyak Mimba (Azadirachta Indica) Dan Minyak Jarak Kepyar (Ricinus Communis) Terhadap Spodoptera Frugiperda. Agrikultura, 32(3), 207.

Zaid, R., Canela-Garayoa, R., Ortega-Chacón, N. M., & Mouhouche, F. (2022). Phytochemical Analyses And Toxicity Of Nerium Oleander (Apocynaceae) Leaf Extracts Against Chaitophorus Leucomelas Koch, 1854 (Homoptera: Aphididae). Journal Of The Saudi Society Of Agricultural Sciences, 21(5), 310–317. Https://Doi.Org/10.1016/J.Jssas.2021.10.011

Zatelli, A., Fondati, A., Maroli, M., Leishmaniosis, C., & Group, W. (2022). The Knowns And Unknowns Of The Efficacy Of Neem Oil (Azadirachta Indica) Used As A Preventative Measure Against Leishmania Sand Fly Vectors (Phlebotomus Genus). Preventive Veterinary Medicine, 202, 105618.

Author Biographies

Supriatno, Syiah Kuala University

Nur Asiah, Syiah Kuala University

Asiah M D, Syiah Kuala University

Djufri, Syiah Kuala University

Safrida, Syiah Kuala University


Copyright (c) 2023 Supriatno, Nur Asiah, Asiah M D, Djufri, Safrida

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).