Dual Inoculation of Rhizobium and Arbuscular Mycorrhizal Fungi Increases Soil-Total Nitrogen, Available Phosphorus, and Yield of Soybean in Vertisols

Authors

Lily F Ishaq , Ingracia J. A. Manehat , Anthonius S. J. Adu Tae , Yoke I. Benggu

DOI:

10.29303/jppipa.v9i5.3162

Published:

2023-05-31

Issue:

Vol. 9 No. 5 (2023): May

Keywords:

Arbuscular mycorrhizal fungi, Soybean, Rhizobium, Vertisols

Research Articles

Downloads

How to Cite

Ishaq, L. F., Manehat, I. J. A. ., Tae, A. S. J. A. ., & Benggu, Y. I. . (2023). Dual Inoculation of Rhizobium and Arbuscular Mycorrhizal Fungi Increases Soil-Total Nitrogen, Available Phosphorus, and Yield of Soybean in Vertisols. Jurnal Penelitian Pendidikan IPA, 9(5), 2444–2451. https://doi.org/10.29303/jppipa.v9i5.3162
Crossref
Scopus
Google Scholar
Europe PMC

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

Soybean is a protein source food crop with high economic value. So far, national production has not been able to meet domestic demand resulting in continuing import of soybean. The use of rhizobium and arbuscular mycorrhizal fungi (AMF) as biological fertilizers could be an alternative to increase soybean production. The research aimed to investigate the effect of dual inoculation of rhizobium and arbuscular mycorrhizal fungi (AMF) on root nodules formation, AMF development, total-N and available P of Vertisols, and yield of soybean. This study was designed in a completely randomized design) with 4 treatments namely no inoculation (R0), rhizobium inoculation (R1), AMF inoculation (R2), and dual inoculation of rhizobium and AMF (R3), with four replications. The variables observed were the number of effective root nodules, AMF spore density, AMF colonization, soil total-N, available P, plant height, number of pods, and seed weight. The results showed that dual inoculation of rhizobium and AMF had a better effect on the number of effective root nodules, AMF spore density, AMF colonization, soil total- N, available P, plant height, number of pods, and seed weight compared to the single inoculation and un-inoculated treatments

References

Abd-Alla, M. H., El-Enany, A. W. E., Nafady, N. A., Khalaf, D. M., & Morsy, F. M., (2014). Symbiotic interaction of Rhizobium leguminosarum bv viciae and arbuscular mycorrhizal fungi as plant growth promoting biofertilizers for faba bean (Vicia faba L.) in alkaline soils. Microbiology Reserach, 169, 49–58. http://doi: 10.1016/j.micres.2013.07.007.

Adeyemi, N. O., Atayese, M. O., Olubede, A. A., & Akan, M., E. (2020). Effect of commercial arbuscular mycorrhizal fungi inoculant on growth and yield of soybean under controlled and natural field conditions. Journal of Plant Nutrition, 43(4), 487-499. https://doi.org/10.1080/01904167.2019.1685101

Allito, B. B, Ewusi-Mensah, N., & Logah, V. (2020). Legume-Rhizobium strain specificity enhancances nutrition and nitrogen fixation in faba bean (Vicia faba L.). Agronomi, 10, 826. https://doi:10.3390/agronomy10060826

Ashwin, R., Bagyaraj, D.J., & Raju, B. M. (2022). Dual inoculation with rhizobia and arbuscular mycorrhizal fungus improves water stress tolerance and productivity in soybean. Plant Stress, 4. https://doi.org/10.1016/j.stress.2022.100084

Brundrett, M., Bougher, N., Dell, B., Grove, T., & Malajczuk, N. (1996). Working with Mycorrhiza in Forestry and Agriculture. Australian Centre for International Agricultural Research.

Cely, M. V. T., de Oliveira, A. G., de Freitas, V. F., de Luca, M. B., Barazetti, A. R., dos Santos, I. M. O., Gionco,B., Garcia, G. V., Prete, C. E. C., & Andrade, G. (2016). Inoculant of arbuscular mycorrhizal fungi (Rhizophagus clarus) increase yield of soybean and cotton under field condition. Frontier in Microbiology, 7, 720. https://doi: 10.3389/fmicb.2016.00720

Dobo, B. (2022). Effect of arbuscular mycorrhizal fungi (AMF) and rhizobium inoculation on growth and yield of Glycine max L. varieties. International Journal of Agronomy, 22. https://doi.org/10.1155/2022/9520091

Hardjowigeno, S. (1995). Klasifikasi Tanah dan Pedogenesis. Akademika Pressindo, Jakarta.

Hashem, A., Abd_Allah, E. F., Alqarawih, A. A., Wirth, S., & Egamberdieva, D. (2016). Comparing symbiotic performance and physiological responses of two soybean cultivars to arbuscular mycorrhizal fungi under salt stress. Saudi Journal of Biological Sciences, 26(1), 38-48. https://doi.org/10.1016/j.sjbs.2016.11.015

Havugimana, E., Bhople, B. S., Byringiro, E., & Mugabo, J. P. (2016). Role of dual inoculation rhizobium and arbuscular mycorrhizal (AM) fungi on pulse crops production. Walailak Journal, 13(1), 1-7. Retrieved from https://wjst.wu.ac.th/index.php/wjst/article/view/1559

Iglehon, O. N., & Babalola, O. O. (2021). Rhizobium and mycorrhizal fungal species improved soybean Yield under drought stress conditions. Current Microbiology, 78, 1615-1627. https://doi.org/10.1007/s00284-021-02432-w

Jumrani, K., Bhatia, V. S., Kataria, S., Alamri, S. A., Shiddiqui, M. H., & Rastogi, A. (2022). Inoculation with arbuscular mycorrhizal fungi alleviates the adverse effects of high temperature in soybean. Plants, 11, 2210. https://doi.org/10.3390/plants11172210

Kaschuk, G., Kuyper, T. W., Leffelaar, P. A., Hungria, M., & Giller, K. E. (2009). Are the rates of photosynthesis stimulated by the carbon sink strength of rhizobial and arbuscular mycorrhizal symbioses? Soil Biology and Biochemistry, 41(6), 1233-1244. https://doi.org/10.1016/j.soilbio.2009.03.005

Lotfabadi Z. E., Wesany, W., & Tahir, N. A. (2022). Arbuscular mycorrhizal fungi species improve the fatty acids profile and nutrient status of soybean cultivars grown under drought stress. Journal of Applied Microbiology, 132(3), 2177-2188. https://doi.org/10.1111/jam.15326

Macik, M., Gryta, A., & Frac, M. (2020). Chapter Two – Biofertilizer in agriculture: An overview on concepts, strategies and effects on soil microorganism. Advances in Agronomy, 162, 31-87. http.//doi.org/10.1016/bs.agron.2020.02.001

Marschner, H., & Dell, B. (1994). Nutrient uptake in mycorrhizal symbiosis. Plant and Soil, 159(1), 89-102. https://doi.org/10.1007/BF00000098

Meng, L., Zhang, A., Wang, F., Han, X., Wang, D., & Li, S. (2015). Arbuscular mycorrhizal fungi and rhizobium facilitate nitrogen uptake and transfer in soybean/maize intercropping systems. Frontiers in Plant Science. 6(339). http://doi/ 10.3389/fpls.2015.00339

Ningrum, I.H., Irianto, H., & Risptani, E.W. (2018). Analysis of soybean production and import trends and its import factors in Indonesia. IOP Conference Series: Earth and Environmental Sciences, 142(1). http://doi.org/10.1088/1755-1315/142/1/012059

O’Keefe, S., Bianchi, L., & Sharman, J. (2015). Soybean nutirion. SM Journal of Nutrition and Metabolism, 1(2). 1006. Retrieved from https://vtechworks.lib.vt.edu/bitstream/handle/10919/73053/SoybeanNutrition.pdf?se

Silva-Flores, P., Bueno, C. G., Neira, J., & Plafner, G. (2019). Factors affecting arbuscular mycorrhizal fungi spore density in the Chilean Mediterranean- type ecosystem. Journal of Soil Science and Plant Nutrition, 19, 42-50. https://doi.org/10.1007/s42729-018-0004-6

Smith, S. E., & Read, D. J. (2008). Mycorrhizal Symbiosis. Akademic Press. California USA.

Tisdale, S., Nelson, W., Havlin, J., & Beaton, J. (1999). Soil Fertility and Fertilizers. An introduction to Nutrient Management. 6th Edition. Prentice-Hall New Jersey.

Wang, X., Pan, Q., Chne, F., Yan, X., & Liao, H. (2011). Effects of co-inoculation with arbuscular mycorrhizal fungi and rhizobia on soybean growth as related to root architecture and availability of N and P. Mycorrhiza, 21, 173-181. https://doi.org/10.1007/s00572-010-0319-1

Wang, G., Li, X., Christie, P., Zang, J., & Li, X. (2016). Response of arbuscular mycorrhizal fungi to soil phosphorus patches depends on context. Crop Pasture Science, 67, 1116-1125. https//doi.org/ 10.1071/CP16168

Wardhani, Y., Yulian, A. I., & Munir, M. M. (2019). Potensi mikoriza indigenous terhadap serapan P (fosfor) di tanah latosol pada tanaman kedelai (Glycine max L. merril) varietas anjasmoro. EPiC, 1(2), 83-86. Retrieved from http://ojs.unwaha.ac.id/index.php/epic/article/view/59

Yang, J., Lan, L., Jin, Y., Yu, N., Wand, D., & Wang, E. (2022). Mechanisms underlying legume-rhizobium symbioses. Journal of Integrative Plant Biology, 64, 244-267. https://doi.org/10.1111/jipb.13207

Author Biographies

Lily F Ishaq, Universitas Nusa Cendana

Ingracia J. A. Manehat, Nusa Cendana University

Anthonius S. J. Adu Tae, Nusa Cendana University

Yoke I. Benggu, Nusa Cendana University

License

Copyright (c) 2023 Lily Ishaq

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).