Study of the Utilization of Liquid Organic Fertilizer from Leachate from Tlekung Landfill, Batu City Using Ecoenzyme as Biodegradation of Heavy Metals (Pb, Cu)

Authors

Luluk Indri Astuti , Arie Srihardyastutie , Qonitah Fardiyah

DOI:

10.29303/jppipa.v11i2.3466

Published:

2025-02-25

Issue:

Vol. 11 No. 2 (2025): February

Keywords:

Bioremediation, Ecoenzyme, Leachate, Liquid Organic Fertilizer

Research Articles

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Astuti, L. I., Srihardyastutie, A., & Fardiyah, Q. (2025). Study of the Utilization of Liquid Organic Fertilizer from Leachate from Tlekung Landfill, Batu City Using Ecoenzyme as Biodegradation of Heavy Metals (Pb, Cu). Jurnal Penelitian Pendidikan IPA, 11(2), 84–91. https://doi.org/10.29303/jppipa.v11i2.3466

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Abstract

Leachate is water produced from the waste processing process at the final disposal site (TPA). Leachate contains various organic substances, so many local people use leachate as fertilizer. However, on the other hand, leachate contains heavy metals. As an effort to reduce the content of heavy metals such as Pb and Cu, biological remediation techniques are used by adding ecoenzymes. This study aims to determine the effectiveness of ecoenzymes on leachate at the Tlekung TPA, Batu City. Leachate at the inlet point of Tlekung Landfill, Batu City was taken as much as 20 liters and the volume of ecoenzyme was varied, namely P1 (leachate 1000 ml), P2 (leachate 1000 ml + 1 ml ecoenzyme), P3 (leachate 1000 ml + 5 ml ecoenzyme), P4 (leachate 1000 ml + 10 ml ecoenzyme), P5 (leachate 1000 ml + 15 ml ecoenzyme). The research stages were carried out by testing the pH using a pH meter, testing the number of colonies using the Total Plate Count (TPC) method, testing the levels of lead (Pb) and copper (Cu) using the AAS method. The results showed that the addition of ecoenzyme to leachate affected the pH value, increased the number of colonies and decreased the levels of Pb and Cu metals. The addition of 10 mL of ecoenzyme in 1000 ml of leachate in the P4 treatment could increase the number of colonies and reduce the levels of Pb and Cu.

References

Abubakar, I. R., Maniruzzaman, K. M., Dano, U. L., AlShihri, F. S., AlShammari, M. S., Ahmed, S. M. S., Al-Gehlani, W. A. G., & Alrawaf, T. I. (2022). Environmental Sustainability Impacts of Solid Waste Management Practices in the Global South. International Journal of Environmental Research and Public Health, 19(19), 12717. https://doi.org/10.3390/ijerph191912717

Akhtar, M. S., Ali, S., & Zaman, W. (2024). Innovative Adsorbents for Pollutant Removal: Exploring the Latest Research and Applications. Molecules, 29(18), 4317. https://doi.org/10.3390/molecules29184317

Angon, P. B., Islam, Md. S., Kc, S., Das, A., Anjum, N., Poudel, A., & Suchi, S. A. (2024). Sources, effects and present perspectives of heavy metals contamination: Soil, plants and human food chain. Heliyon, 10(7), e28357. https://doi.org/10.1016/j.heliyon.2024.e28357

Anuardo, R. G., Espuny, M., Costa, A. C. F., & Oliveira, O. J. (2022). Toward a cleaner and more sustainable world: A framework to develop and improve waste management through organizations, governments and academia. Heliyon, 8(4), e09225. https://doi.org/10.1016/j.heliyon.2022.e09225

Briffa, J., Sinagra, E., & Blundell, R. (2020). Heavy metal pollution in the environment and their toxicological effects on humans. Heliyon, 6(9), e04691. https://doi.org/10.1016/j.heliyon.2020.e04691

Cardoso, J., Gomes, H. T., & Brito, P. (2019). Viability of the Use of Leachates from a Mechanical Biological Municipal Solid Waste Treatment Plant as Fertilizers. Recycling, 4(1), 8. https://doi.org/10.3390/recycling4010008

Chen, H., Xu, H., Zhong, C., Liu, M., Yang, L., He, J., Sun, Y., Zhao, C., & Wang, D. (2024). Treatment of landfill leachate by coagulation: A review. Science of The Total Environment, 912, 169294. https://doi.org/10.1016/j.scitotenv.2023.169294

Chen, X., Ren, Y., Li, C., Shang, Y., Ji, R., Yao, D., & He, Y. (2024). Study on Factors Influencing the Migration of Heavy Metals from Soil to Vegetables in a Heavy Industry City. Sustainability, 16(24), 11084. https://doi.org/10.3390/su162411084

Dogan, E., Hodžić, S., & Šikić, T. F. (2022). A way forward in reducing carbon emissions in environmentally friendly countries: The role of green growth and environmental taxes. Economic Research-Ekonomska Istraživanja, 35(1), 5879–5894. https://doi.org/10.1080/1331677X.2022.2039261

Elleuch, B., Bouhamed, F., Elloussaief, M., & Jaghbir, M. (2018). Environmental sustainability and pollution prevention. Environmental Science and Pollution Research, 25(19), 18223–18225. https://doi.org/10.1007/s11356-017-0619-5

Elnajdi, A., Berland, A., Haeft, J., & Dowling, C. (2023). Influence of Soil pH, Organic Matter, and Clay Content on Environmentally Available Lead in Soils: A Case Study in Muncie, Indiana, USA. Open Journal of Soil Science, 13(10), 414–430. https://doi.org/10.4236/ojss.2023.1310019

El-Saadony, M. T., Saad, A. M., El-Wafai, N. A., Abou-Aly, H. E., Salem, H. M., Soliman, S. M., Abd El-Mageed, T. A., Elrys, A. S., Selim, S., Abd El-Hack, M. E., Kappachery, S., El-Tarabily, K. A., & AbuQamar, S. F. (2023). Hazardous wastes and management strategies of landfill leachates: A comprehensive review. Environmental Technology & Innovation, 31, 103150. https://doi.org/10.1016/j.eti.2023.103150

Enya, O., Heaney, N., Iniama, G., & Lin, C. (2020). Effects of heavy metals on organic matter decomposition in inundated soils: Microcosm experiment and field examination. Science of The Total Environment, 724, 138223. https://doi.org/10.1016/j.scitotenv.2020.138223

Hosseini Beinabaj, S. M., Heydariyan, H., Mohammad Aleii, H., & Hosseinzadeh, A. (2023). Concentration of heavy metals in leachate, soil, and plants in Tehran’s landfill: Investigation of the effect of landfill age on the intensity of pollution. Heliyon, 9(1), e13017. https://doi.org/10.1016/j.heliyon.2023.e13017

Jagaba, A. H., Kutty, S. R. M., Lawal, I. M., Abubakar, S., Hassan, I., Zubairu, I., Umaru, I., Abdurrasheed, A. S., Adam, A. A., Ghaleb, A. A. S., Almahbashi, N. M. Y., Al-dhawi, B. N. S., & Noor, A. (2021). Sequencing batch reactor technology for landfill leachate treatment: A state-of-the-art review. Journal of Environmental Management, 282, 111946. https://doi.org/10.1016/j.jenvman.2021.111946

Janeeshma, E., Habeeb, H., Sinha, S., Arora, P., Chattaraj, S., Das Mohapatra, P. K., Panneerselvam, P., & Mitra, D. (2024). Enzymes-mediated solid waste management: A sustainable practice for recycling. Waste Management Bulletin, 1(4), 104–113. https://doi.org/10.1016/j.wmb.2023.10.007

Kang, C.-U., Ji, S.-E., Pabst, T., Choi, K.-W., Khan, M. A., Kumar, R., Krishnaiah, P., Han, Y., Jeon, B.-H., & Kim, D.-H. (2021). Copper Extraction from Oxide Ore of Almalyk Mine by H2SO4 in Simulated Heap Leaching: Effect of Particle Size and Acid Concentration. Minerals, 11(9), 1020. https://doi.org/10.3390/min11091020

Khader, E. H., Mohammed, T. J., & Adnan, S. W. (2021). Reduction of oil and COD from produced water by activated carbon, zeolite, and mixed adsorbents in a fixed-bed column. Desalination and Water Treatment, 227, 216–227. https://doi.org/10.5004/dwt.2021.27295

Kuppan, N., Padman, M., Mahadeva, M., Srinivasan, S., & Devarajan, R. (2024). A comprehensive review of sustainable bioremediation techniques: Eco friendly solutions for waste and pollution management. Waste Management Bulletin, 2(3), 154–171. https://doi.org/10.1016/j.wmb.2024.07.005

Liaquat, I., Munir, R., Abbasi, N. A., Sadia, B., Muneer, A., Younas, F., Sardar, M. F., Zahid, M., & Noreen, S. (2024). Exploring zeolite-based composites in adsorption and photocatalysis for toxic wastewater treatment: Preparation, mechanisms, and future perspectives. Environmental Pollution, 349, 123922. https://doi.org/10.1016/j.envpol.2024.123922

Lucero-Sobarzo, D., Beltrán-Villavicencio, M., González-Aragón, A., & Vázquez-Morillas, A. (2022). Recycling of nutrients from landfill leachate: A case study. Heliyon, 8(5), e09540. https://doi.org/10.1016/j.heliyon.2022.e09540

Ma, X. (2024). Heavy metals remediation through lactic acid bacteria: Current status and future prospects. Science of The Total Environment, 946, 174455. https://doi.org/10.1016/j.scitotenv.2024.174455

Ma, Z., Zuo, T., Frey, N., & Rangrez, A. Y. (2024). A systematic framework for understanding the microbiome in human health and disease: From basic principles to clinical translation. Signal Transduction and Targeted Therapy, 9(1), 237. https://doi.org/10.1038/s41392-024-01946-6

Mafe, A. N., Edo, G. I., Makia, R. S., Joshua, O. A., Akpoghelie, P. O., Gaaz, T. S., Jikah, A. N., Yousif, E., Isoje, E. F., Igbuku, U. A., Ahmed, D. S., Essaghah, A. E. A., & Umar, H. (2024). A review on food spoilage mechanisms, food borne diseases and commercial aspects of food preservation and processing. Food Chemistry Advances, 5, 100852. https://doi.org/10.1016/j.focha.2024.100852

Martínez-Zamudio, L. Y., González-González, R. B., Araújo, R. G., Rodríguez Hernández, J. A., Flores-Contreras, E. A., Melchor-Martínez, E. M., Parra-Saldívar, R., & Iqbal, H. M. N. (2024). Emerging pollutants removal from leachates and water bodies by nanozyme-based approaches. Current Opinion in Environmental Science & Health, 37, 100522. https://doi.org/10.1016/j.coesh.2023.100522

Mukherjee, S., Sarkar, B., Aralappanavar, V. K., Mukhopadhyay, R., Basak, B. B., Srivastava, P., Marchut-Mikołajczyk, O., Bhatnagar, A., Semple, K. T., & Bolan, N. (2022). Biochar-microorganism interactions for organic pollutant remediation: Challenges and perspectives. Environmental Pollution, 308, 119609. https://doi.org/10.1016/j.envpol.2022.119609

Natasya, N., Fadilah, M., Fitri, R., Farma, S. A., Raharjeng, A. R. P., & Simwela, M. (1970). Analysis of Eco-enzyme Quality Based on Differences in Plant Tissue. Jurnal Biota, 9(1), 45–53. https://doi.org/10.19109/Biota.v9i1.13166

Pasalari, H., Moosavi, A., Kermani, M., Sharifi, R., & Farzadkia, M. (2024). A systematic review on garbage enzymes and their applications in environmental processes. Ecotoxicology and Environmental Safety, 277, 116369. https://doi.org/10.1016/j.ecoenv.2024.116369

Pikuła, D., & Stępień, W. (2021). Effect of the Degree of Soil Contamination with Heavy Metals on Their Mobility in the Soil Profile in a Microplot Experiment. Agronomy, 11(5), 878. https://doi.org/10.3390/agronomy11050878

Rashid, A., Schutte, B. J., Ulery, A., Deyholos, M. K., Sanogo, S., Lehnhoff, E. A., & Beck, L. (2023). Heavy Metal Contamination in Agricultural Soil: Environmental Pollutants Affecting Crop Health. Agronomy, 13(6), 1521. https://doi.org/10.3390/agronomy13061521

Sanjaya, A. P., Praseptiangga, D., Zaman, M. Z., Umiati, V. F., & Baraja, S. I. (2023). Effect of pH, temperature, and salt concentration on the growth of Bacillus subtilis T9-05 isolated from fish sauce. IOP Conference Series: Earth and Environmental Science, 1200(1), 012050. https://doi.org/10.1088/1755-1315/1200/1/012050

Tang, H., Xiang, G., Xiao, W., Yang, Z., & Zhao, B. (2024). Microbial mediated remediation of heavy metals toxicity: Mechanisms and future prospects. Frontiers in Plant Science, 15, 1420408. https://doi.org/10.3389/fpls.2024.1420408

Tang, L., Tang, M., & Xi, W. (2023). Has building innovative provinces reduce environmental pollution?—Evidence from a quasi-natural experiment in China. Frontiers in Environmental Science, 11, 1177478. https://doi.org/10.3389/fenvs.2023.1177478

Vaverková, M. D., Elbl, J., Koda, E., Adamcová, D., Bilgin, A., Lukas, V., Podlasek, A., Kintl, A., Wdowska, M., Brtnický, M., & Zloch, J. (2020). Chemical Composition and Hazardous Effects of Leachate from the Active Municipal Solid Waste Landfill Surrounded by Farmlands. Sustainability, 12(11), 4531. https://doi.org/10.3390/su12114531

Wang, J., & Qiao, Z. (2024). A comprehensive review of landfill leachate treatment technologies. Frontiers in Environmental Science, 12, 1439128. https://doi.org/10.3389/fenvs.2024.1439128

Wdowczyk, A., & Szymańska-Pulikowska, A. (2020). Differences in the Composition of Leachate from Active and Non-Operational Municipal Waste Landfills in Poland. Water, 12(11), 3129. https://doi.org/10.3390/w12113129

Williamson, A. J., Verbruggen, F., Chavez Rico, V. S., Bergmans, J., Spooren, J., Yurramendi, L., Laing, G. D., Boon, N., & Hennebel, T. (2021). Selective leaching of copper and zinc from primary ores and secondary mineral residues using biogenic ammonia. Journal of Hazardous Materials, 403, 123842. https://doi.org/10.1016/j.jhazmat.2020.123842

Yan, A., Wang, Y., Tan, S. N., Mohd Yusof, M. L., Ghosh, S., & Chen, Z. (2020). Phytoremediation: A Promising Approach for Revegetation of Heavy Metal-Polluted Land. Frontiers in Plant Science, 11, 359. https://doi.org/10.3389/fpls.2020.00359

Yeboah, J. O., Shi, G., & Shi, W. (2021). Effect of Heavy Metal Contamination on Soil Enzymes Activities. Journal of Geoscience and Environment Protection, 09(06), 135–154. https://doi.org/10.4236/gep.2021.96008

Zhou, B., Zhang, T., & Wang, F. (2023). Microbial-Based Heavy Metal Bioremediation: Toxicity and Eco-Friendly Approaches to Heavy Metal Decontamination. Applied Sciences, 13(14), 8439. https://doi.org/10.3390/app13148439

Author Biographies

Luluk Indri Astuti, Universitas Brawijaya

Arie Srihardyastutie, Universitas Brawijaya

Qonitah Fardiyah, Universitas Brawijaya

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