Effect of the Thermophilic Bacterial Biculture Consortium from Mudiak Sapan Hot Springs on Biofuel Production

Authors

Irdawati , Fahra , Dwi Hilda Putri , Dezi Handayani , Yusrizal

DOI:

10.29303/jppipa.v9i10.3597

Published:

2023-10-25

Issue:

Vol. 9 No. 10 (2023): October

Keywords:

Bacteria consortium, Bioethanol, Biofuel, Thermophilic bacteria

Research Articles

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Irdawati, I., Fahra, F., Putri, D. H. ., Handayani, D. ., & Yusrizal, Y. (2023). Effect of the Thermophilic Bacterial Biculture Consortium from Mudiak Sapan Hot Springs on Biofuel Production. Jurnal Penelitian Pendidikan IPA, 9(10), 9032–9037. https://doi.org/10.29303/jppipa.v9i10.3597
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Abstract

Biofuels are fuels derived from biomass and consist of biogas, biodiesel and bioethanol. Bioethanol is a biofuel whose main ingredients are from plants and generally use microorganisms in the fermentation process. One way is to use thermophilic bacteria with the advantage of low contamination levels and better product quality. Bacteria that exist in nature are not only in a single form but also exist in the form of a consortium in which there are beneficial or detrimental bacterial interactions. Favorable bacterial interactions indicate that the bacteria are compatible. Using a consortium with compatible bacteria gives better results than using a single bacteria. This study aims to determine the compatibility and effect of a consortium of thermophilic bacteria from Mudiak Sapan hot springs on biofuel yields. This research is a descriptive research. To test the cooperation between isolates, a compatibility test was carried out using the disk diffusion method. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%.

References

Agusri, R. (2022). Optimization of Thermophilic Bacteria Temperature in Producing Bioethanol as Biofuel. Padang State University. Retrieved from http://repository.unp.ac.id/39806/

Asri, A. C., & Zulaika, E. (2016). Synergism Between Consortium Azotobacter Isolates. Journal of Science and Arts ITS, 5(2), 2337–3520. https://doi.org/10.12962/j23373520.v5i2.20693

Deng, Y.-J., & Wang, S. Y. (2016). Synergistic growth in bacteria depends on substrate complexity. Journal of Microbiology, 54(1), 23–30. https://doi.org/10.1007/s12275-016-5461-9

Fifendy, M., Irdawati, I., & Yenti, N. (2015). Screening of thermophilic bacteria that produce amylase enzymes from the Sapan Aro hot spring, South Solok Regency. Scientific Periodical Exacts in Mathematics and Natural Sciences, 1, 73–81. Retrieved from http://ejournal.unp.ac.id/index.php/eksakta/article/view/5266

Irdawati. (2019). Diversity of Thermoxilanolytic Bacteria from Habitat of South Solok Hot Springs and Their Potential as Pulp Additives. Andalas University. http://scholar.unand.ac.id/42929/

Irdawati, F. (2011). Isolation of Amylase Producing Thermophilic Bacteria from Rimbo Panti Pasaman Hot Springs. Retrieved from http://repository.unp.ac.id/48/1/3. Penelitian 2011- 2.pdf

Irdawati, I., Syamsuardi, S., Agustien, A., & Alberida, H. (2017). Growth Profile of Alkaline Xylanase Producing Thermophilic Bacteria from Mudiak Sapan Hot Springs, South Solok. Proceedings of the National Seminar on Biology Education. Retrieved from http://repository.unp.ac.id/27774/

Jovanita, L., & Advinda, L. (2022). Compatibility Test of Fluorescent Pseudomonas Isolated from Plant Rhizosphere. Journal of Porch Biology, 7(1), 65–69. Retrieved from https://serambibiologi.ppj.unp.ac.id/index.php/srmb/article/view/21

Kasim, S., & Usman, H. (2013). Utilization of ARS-CHAT Medium in Potential Marine Phytoplankton Biomass Production As Raw Material for Bioethanol-type Biofuels. Proceedings of the MIPA National Seminar. Retrieved from https://ejournal.undiksha.ac.id/index.php/semnasmipa/article/view/2729

Khasanah. (2003). Formulation, Physical-Chemical Characterization and Organoleptic of Sweet Potato Breakfast Food Products [Faculty of Agricultural Technology, IPB]. Retrieved from http://repository.ipb.ac.id/handle/123456789/25878

Komarawidjaja, W., & Lysiastuti, E. (2009). Status of local oil-degrading microbial consortia. Journal of Environmental Technology, 10(3), 347–354. https://doi.org/10.29122/jtl.v10i3.1481

Lynd L, R. (1989). Production of ethanol from lignocellulosic materials using thermophilic bacteria : critical evaluation of potential and review. Adv In Biochem. Eng. Biotechnol. Springer-Verlag, 38, 1–52. https://doi.org/10.1007/BFb0007858

Mayzuhroh, A. (2015). Production of Bioethanol Using Commercial Starters With and Without Providing Agitation Aeration in Molasses Media.

Meganandi, K. A. (2016). Production of Bioethanol from Sorghum Bicolor Stems. Journal of Purification, 16(2). Retrieved from https://purifikasi.id/index.php/purifikasi/article/download/43/41/

Millati, G. Z. (2018). Practical Work Report on the Use of Compatibility Test of 7 LOB Isolates with Pseudomonas fluorescens PF32 Using the Cross Streak and Modified Kirby-Bauer Methods. Microbiology Study Program, Bandung Institute of Technology.

Notodarmojo, S. (2005). Soil and Groundwater Pollution. ITB Publisher.

Novia, A., & Rosmawati, W. (2014). Pembuatan Bioetanol dari Jerami Padi Dengan Metode Ozonolisis–Simultaneous Saccharification and Fermentation (SSF). Journal of Chemical Engineering, 3(20). Retrieved from https://repository.unsri.ac.id/73119/2/180

Ou, M., Mohammed, N., Ingram, L. O., & Shanmugam, K. T. (2009). Thermophilic Bacillus coagulans Requires Less Cellulases for Simultaneous Saccharification and Fermentation of Cellulose to Products than Mesophilic Microbial Biocatalysts. Journal of Applies Biochemistry and Biotechnology, 155, 76–82. https://doi.org/10.1007/s12010-008-8509-4

Prayudyaningsih, R., & Nursyamsi, R. S. (2015). Beneficial Soil Microorganisms in Rooting Rhizosphere Under Community Forest Stands in South Sulawesi. Proceedings of the National Seminar on Community Biodiv Indonesia, 1, 954–959. https://doi.org/10.13057/psnmbi/m010453

Prescott, L. M., Harley, J. P., & Klein, D. A. (2002). Microbiology (5th ed.). McGraw-Hill.

Rifai, M. R., H, W., & Susanto, A. (2020). Synergism and Antagonism of Several Types of Consumed Bacterial Isolates. BIOLOVA, 1(1), 21–26. https://doi.org/10.24127/biolova.v1i1.31

Safari, W. F., & Intercession, M. (2022). Effect of Pretreatment and Composition of Trichoderma Viride and Zymomonas Mobilis Consortium on Bioethanol Production from Leaf Litter. Indonesian Journal of Biotechnology and Biodiversity, 6(2), 52–60. https://doi.org/10.47007/ijobb.v6i2.134

Sari, U. M. (2012). Screening and Characterization of Thermophilic Cellulolytic Bacteria in Medang River Hot Springs, Kerinci, Jambi. Andalas University.

Scully, S. M., & Orlygsson, J. (2015). Recent advances in second generation ethanol production by thermophilic bacteria. Energies, 8(1), 1–30. https://doi.org/10.3390/en8010001

Siahaan, S., M, H., & Hasibuan, R. (2013). Determination of the Optimum Conditions of Temperature and Carbonation Time in Making Charcoal from Rice Husk. Journal of Chemical Engineering, 2(1), 1–6. Retrieved from https://talenta.usu.ac.id/jtk/article/view/1423

Vinotha., U. (2019). Bioethanol Production Using Bacterial Consortia From Waste Cellulosic Waste. Think India Journal. Retrieved from https://thinkingdiaquarterly.org/index.php/think-india/article/view/11440

Widjaja, T., Altway, A., Nurkamidah, S., Endahwati, L., Lini, F. Z., & Oktafia, F. (2016). The Effect of Pretreatment and Variety of Microorganisms to the Production of Ethanol from Coffe Pulp. ARPN. Journal of Engineering and Applied Science, 11(2), 1056–1060. Retrieved from https://www.arpnjournals.org/jeas/research_papers/rp_2016/jeas_0116_3453.pdf

Winaya, R., & Kristanto, P. (2002). Use of Vegetable Oils as Alternative Fuels in Direct Injection Diesel Engines. Journal of Mechanical Engineering, 2, 99 – 103. https://doi.org/10.9744/jtm.4.2.pp.99-103

Zilda, D. S., Kusumarini, A., & Chasanah, E. (2008). Protease Screening and Characterization of P5-A Thermo-Acidophilic Bacteria from Tambarana Hot Springs. Journal of Postharvest and Marine and Fishery Biotechnology, 3(2), 113–121. https://doi.org/10.15578/jpbkp.v3i2.17

Author Biographies

Irdawati, Padang State University

Fahra, Universitas Negeri Padang

Dwi Hilda Putri, Universtitas Negeri Padang

Dezi Handayani, Universtitas Negeri Padang

Yusrizal, Universtitas Negeri Padang

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Copyright (c) 2023 Irdawati, Fahra, Dwi Hilda Putri, Dezi Handayani, Yusrizal

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