Antimicrobial Activity Test of Bitter Melon (Momordica charantia L.) Plant Extract Against Staphylococcus epidermidis, Escherichia coli Bacteria and Candida albicans

Authors

Dewa Ayu Citra Rasmi , Lalu Zulkifli , Nurul Ahadia

DOI:

10.29303/jppipa.v9i2.3699

Published:

2023-02-28

Issue:

Vol. 9 No. 2 (2023): February

Keywords:

Bitter melon (Momordica charantia L.), Antimicrobial activity, Secondary metabolites

Research Articles

Downloads

How to Cite

Rasmi, D. A. C. ., Zulkifli, L. ., & Ahadia, N. . (2023). Antimicrobial Activity Test of Bitter Melon (Momordica charantia L.) Plant Extract Against Staphylococcus epidermidis, Escherichia coli Bacteria and Candida albicans. Jurnal Penelitian Pendidikan IPA, 9(2), 454–458. https://doi.org/10.29303/jppipa.v9i2.3699

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

Bitter gourd (Momordica charantia L.) contains secondary metabolites of flavonoids, alkaloids, saponins, and steroids that act as antimicrobials. This study aimed to determine the antimicrobial activity of bitter melon (Momordica charantia L.) plant extract in N-hexane and ethanol solvents against Staphylococcus epidermidis, Escherichia coli, and Candida albicans. This research is an experimental laboratory study, namely the antimicrobial activity test of bitter melon plant extract on the growth of test bacteria using the agar diffusion method. The study's experimental design used a Completely Randomized Design with three repetitions at three concentration variations, namely 30%, 45%, and 60%. Then phytochemical screening was carried out to determine the active compounds in the bitter melon plant extract. Data on the inhibitory power of bitter melon plant extract were analyzed qualitatively, and the differences between concentrations were seen using the ANOVA (Analysis of variance) test. The results showed that bitter melon (Momordica charantia L.) plant extract in ethanol solvent showed activity against the growth of Staphylococcus epidermidis, Escherichia coli bacteria with a concentration of 60%, producing a powerful inhibition zone.

References

Neal, M.J. (2006). Farmakologi Medis Edisi ke-5. Erlangga. ISBN: 979-781-436-X.

Muntasir., Widy, S.A., Andi, I.H., Priska, E.T., Makkasau., Reni, Y.S., Stefany, F., Theresia, M.W. (2022). Antibiotik Dan Resistensi Antibiotik. Yogyakarta: Rizmedia Pustaka Indonesia. ISBN: 9786239873363, 6239873365.

Kementrian Kesehatan Republik Indonesia. (2011). Pedoman Penggunaan Antibiotik. Jakarta: Departemen Kesehatan RI.

Alfermann, R.V.A.W. (2000). Metabolic engineering of plant secondary metabolism. Berlin Heidelberg: Springer.

Maiti, R.K., Pratik, S., Dasari, R. (2012). Crop Plant Anatomy. Wallingford: CAB International. ISBN: 9781780640198.

Sambamurty, A.V.S.S. 2005. Taxonomy Of Angiosperms. New Delhi: LK. International Pvt. Ltd.

Azizah, Z., Zulharmita., Siska W.W. (2018). Skrining Fitokimia Dan Penetapan Kadar Flavonoid Total Ekstrak Etanol Daun Pare (Momordica charantia L.). Jurnal Farmasi Higea. 10 (2): 163-172. DOI: http://dx.doi.org/10.52689/higea.v10i2.212

Dheasabel, G dan Muhammad, A. (2018). Kemampuan Ekstrak Buah Pare Terhadap Kematian Nyamuk Aedes aegypti. Higeia. 2 (2):331-341. https://doi.org/10.15294/higeia.v2i2.20866

Kumar, K.P.S dan Debjit, B. (2010). Tradicional Medicinal Uses And Therapeutic Benefits Of Momordica charantia Linn. International Journal of Pharmaceutical Sciences Review and Research. 4(3): 23-28. Retrieved from https://globalresearchonline.net/journalcontents/volume4issue3/Article%20004.pdf

Riferty, F., Endah, R.E.S., Undang, A.D. (2018). Uji Aktivitas Antibakteri Ekstrak Dan Fraksi Biji Pare (Momordica charantia L.) Terhadap Propionibacterium acnes. Jurnal Ilmiah Farasi Farmasyifa . 1(2): 119-125. https://doi.org/10.29313/jiff.v1i2.3139

Savira, M.D. (2021). Efektivitas Ekstrak Etanol Daun Pare (Momordica charantia L.) Terhadap Pertumbuhan Streptococcus pyogenes Secara In Vitro. Dectoral Dissertation. Universitas Nahdatul Ulama Surabaya. URL: http://repository.unusa.ac.id/id/eprint/7240

Davis, W.W dan T.R Stout. (1971). Disc Plate Method of Microbiological Antibiotic Assay. Microbiology. 22 (4): 659-665. http://dx.doi.org/10.1128/am.22.4.659-665.1971

Katuk, R.H.H., Sesilia A.W., Pemmy T. (2019). Pengaruh Perbedaan Ketinggian Tempat Terhadap Kandungan Metabolit Sekunder Pada Gulma Babandotan (Ageratum conyzoides L.). E-Journal Unsrat. 1(4): 1-6. https://doi.org/10.35791/cocos.v1i4.24162

Hamdani, N.A., Ansari N.F., Fdil R., Abbouyi A.E., Khyai S.E. (2016). Antifungal activity of the alkaloids extracts from aerial parts of Retama monosperma. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 7(2): 969. Retrieved from https://www.rjpbcs.com/pdf/2016_7(2)/[133].pdf

Putri, D.R., Maharani T.A., Evie R. (2019). Aktivitas Antifungi Ekstrak Buah Pare (Momordica charantia L.) Dalam Menghambat Pertumbuhan Jamur Fusarium oxysporum. LenteraBio. 8(2): 156-161. Retrieved from https://ejournal.unesa.ac.id/index.php/lenterabio/article/view/28640

Isramilda., Sukma S., Andi I.S. (2020). Uji Konsentrasi Daya Hambat Rebusan Daun Srikaya (Annona squamosa L.) Terhadap Pertumbuhan Staphylococcus aureus. Best Journal Biology Education Sciene and Technology. 3(1): 1-8. https://doi.org/10.30743/best.v3i1.2378

Author Biographies

Lalu Zulkifli, Biology Education Department, Faculty of Teacher Training and Education, University of Mataram

Nurul Ahadia, Biology Education Department, Faculty of Teacher Training and Education, University of Mataram

License

Copyright (c) 2023 Dewa Ayu Citra Rasmi, Lalu Zulkifli, Nurul Ahadia

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).