Rat Taro Extract (Typhonium flagelliforme (Lodd.)) as A Biofungicide Against the Cause of Leaf Fall (Pestalotiopsis sp.) on Rubber Plants (Hevea brasiliensis)

Authors

Zulheri Noer , Prendy Jonringga Manik , Saipul Sihotang

DOI:

10.29303/jppipa.v9i6.3903

Published:

2023-06-25

Issue:

Vol. 9 No. 6 (2023): June

Keywords:

Bioactive, Biofungicide, Extract, Pestalotiopsis

Research Articles

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How to Cite

Noer, Z. ., Manik, P. J. ., & Sihotang, S. . (2023). Rat Taro Extract (Typhonium flagelliforme (Lodd.)) as A Biofungicide Against the Cause of Leaf Fall (Pestalotiopsis sp.) on Rubber Plants (Hevea brasiliensis). Jurnal Penelitian Pendidikan IPA, 9(6), 4699–4704. https://doi.org/10.29303/jppipa.v9i6.3903
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Abstract

Typhonium flagelliforme or known as rat taro is a herbaceous plant that belongs to the taro tribe. The results of the phytochemical analysis of the leaves and tuber extracts of rat taro are known to contain secondary metabolite compounds belonging to the class of flavonoids, alkaloids, triterpenoids, steroids, saponins, and tannins which act as antimicrobials. This study aims to determine the effectiveness of leaf, stem and tuber extracts of rat taro (Typhonium flagelliforme (Lodd)) as a biofungicide against the cause of leaf fall (Pestalotiopsis sp.) on rubber plants (Hevea brasiliensis). Leaf samples came from rubber plants at PTPN III Karet Sei Putih, Galan District, Deli Serdang Regency, North Sumatra. Pestalotiopsis sp. was isolated from plant parts showing leaf fall symptoms. Pieces of rubber leaves infected with Pestalotiopsis sp. was grown on PDA media in a petri dish and incubated for ±7 days at room temperature 26–28oC. After incubation, microscopic identification of the conidia of the fungus was carried out. In vitro test of the inhibition power of extracts of leaves, stems and tubers of rat taro against the fungus Pestalotiopsis sp. was carried out in a petri dish using the fungus culture method. The results of the phytochemical screening showed that the leaf, stem and tuber extracts of rat taro contained chemical compounds of flavonoids, tannins, saponins, alkoloids, and steroids/triterpenoids. The results showed that the leaves, stems and tuber extracts of rat taro (T. flagelliforme L.) have potential as biofungicides, K13 treatment of tuber extract of rat taro (Typhonium flagelliforme L.) 9% was the best treatment which could inhibit the growth of Pestalotiopsis sp.

References

Badan Pusat Statistik Nasional. (2019). Statistik Karet Indonesia 2019. Jakarta: Badan Pusat Statistik.

Barnet, H. L., & Hunter, B. B. (1972). Illustrated General of Imperfect Fungi (Third Edition). Minneapolis: Minnesota Burgess Publishing Company.

Direktorat Jenderal Perkebunan Kementerian Pertanian. (2019). Kebijakan dan Program Pemerintah Dalam Pengendalian Penyakit Gugur Daun Karet. Retrieved from http://ditjenbun.pertanian.go.id

Direktorat Jenderal Perkebunan Kementerian Pertanian. (2019). Pengenalan dan Pengendalian Organisme Pengganggu Tumbuhan (OPT) Tanaman Karet (Revisi 1). Jakarta: Direktorat Perlindungan Perkebunan, Direktorat Jenderal Perkebunan, Kementerian Pertanian.

Farida, Y., Martati, T., Musir, A., & Edward, B. (2010). Uji Aktivitas Sitotoksik dan Antioksidan dari Ekstrak Daun Keladi Tikus (Typhonium divaricatum (L) Decne). Jurnal Ilmu Kefarmasian Indonesia, 8(2), 118-124. Retrieved from http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/download/343/242/

Febbiyanti, T. R., & Fairuza, Z. (2019). Identifikasi Penyebab Kejadian Luar Biasa Penyakit Gugur Daun Karet di Indonesia. Jurnal Penelitian Karet, 37(2), 193-206. https://doi.org/10.22302/ppk.jpk.v37i2.616

Febbiyanti, T. R., Kusdiana, A. P. J., Fairuza, Z., & Herlinawati, E. (2018). The Outbreak of Fusicoccum Leaf Disease in Indonesia and the Potential Yield Loss. International Plant Protection Workshop. Palembang: Indonesian Rubber Research Institute dan International Rubber Research and Development Board.

Firdaus, M. (2015). Manajemen Agribisnis. Jakarta: Bumi Aksara.

Fosgerau, K., & Hoffmann, T. (2015). Peptide Therapeutics: Current Status and Future Directions. Drug Discov Today, 20(1), 122-128. https://doi.org/10.1016/j.drudis.2014.10.003

Gholib, D. (2009). Uji Daya Hambat Daun Senggani terhadap Trychophyton mentagrophytes dan Candida albicans. Berita Biologi: Jurnal Ilmu-Ilmu Hayati, 9(5), 523-528. https://doi.org/10.14203/beritabiologi.v9i5.1989

Gupita, N. A. (2021). Efektivitas Antibakteri Kombinasi Ekstrak Metanol Daun Beringin (Ficus benjamina L.), Daun Tin (Ficus carica L.), dan Daun Karet Kebo (Ficus elastica) terhadap Bakteri Escherichia coli. Skripsi. Universitas Islam Negeri Sunan Ampel, Surabaya.

Hesananda, R., Warnas, H. L. H. S., & Sianipar, N. F. (2017). Supervised Classification Karakter Morfologi Tanaman Keladi Tikus (Typhonium flagelliforme) Menggunakan Database Management System. Jurnal Sistem Komputer, 7(2), 50-58. https://doi.org/10.14710/jsk.v7i2.153

Ismaini, L. (2011). Aktivitas Antifungi Ekstrak (Centella asiatica L.) Urban terhadap Fungi Patogen pada Daun Anggrek (Bulbophyllum flavidiflorum Carr). Jurnal Penelitian Sains, 14(1), 47-50. https://doi.org/10.56064/jps.v14i1.127

Iswantini, D., Syabirin, G., & Affandi, Y. (2009). Daya Hambat Ekstrak Air Etanol Keladi Tikus (Typhonium flagellforme) terhadap Enzim Tirosin Kinase Secara in Vitro. Prosiding Seminar Nasional II, Bogor, 47-58. Retrieved from http://repository.ipb.ac.id/handle/123456789/61938

Mankaran, S., Dinesh, K., Deepak, S., & Gurmeet, S. (2013). Typhonium flagelliforme: A Multipurpose Plant. International Research Journal of Pharmacology, 4(3), 45-48. https://doi.org/10.7897/2230-8407.04308

Mohan S., Wahab S. I. I. A., Abdul A. B., Al-Zubairi A.S., Elhassan M.M. dan Yousif M. (2008). Antibacterial and Antioxidant Activities of Typhonium flagelliforme (Lodd.) Blume Tuber. American Journal of Biochemistry and Biotechnology, 4(4), 402-407. https://doi.org/10.3844/ajbbsp.2008.402.407

Nobakht, G. M., Kadir M. A., & Stansias, J. (2010). Analysis of Preliminary Phytochemical Screening of Typhonium flagelliforme. African Journal of Biotechnology, 9(11), 1655-1657. https://doi.org/10.5897/AJB10.1405

Osbourn, A. E. (1996). Preformed Antimicrobial Compounds and Plant Defense against Fungal Attack. Plant Cell, 8(10), 1821-1831. https://doi.org/10.1105/tpc.8.10.1821

Purnamasari, A. I. (2021). Uji Aktivitas Antifungi Ekstrak Metanol Daun dan Umbi Keladi Tikus (Typhonium flagelliforme) terhadap Pertumbuhan Candida albicans. Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya.

Rusmin, R. (2019). Uji Aktivitas Antimikroba Ekstrak Etanol Daun Keladi Tikus (Typhonium flagelliaforme Blume.) terhadap Pertanaman Staphylococcus aureus dan Escherichia coli. Jurnal Kesehatan Yamasi Makassar, 3(2). Retrieved from https://jurnal.yamasi.ac.id/index.php/Jurkes/article/view/84

Siswandi. (2019). Uji Efektivitas Ekstrak Kulit Jengkol (Pithecellobium jiringa) sebagai Biofungisida terhadap Penyebab Penyakit Layu Fusarium (Fusarium oxysporum) Antraknosa (Colletotrichum capsici) dan Bercak Daun (Cercospora capsici) pada Tanaman Cabai Merah (Capsicum annum L.) Secara In-Vitro. Fakultas Pertanian, Universitas Medan Area.

Sukardi, S. (2011). Identifikasi dan Karakterisasi Umbi Keladi Tikus sebagai Zat Antioksidan Alami. Jurnal GAMMA, 6(2), 143–151. Retrieved from https://ejournal.umm.ac.id/index.php/gamma/article/view/1432

Sukiman, H., & Nuriyanah, N. (2016). Potensi Bakteri Endofitik dari Tanaman Keladi Tikus sebagai Penghasil Zat Antimikroba dan Antioksidan. Biopropal Industri, 7(1), 27-34, https://doi.org/10.36974/jbi.v7i1.833

Syahid, S. F., & Kristina, N. N. (2007). Induksi dan Regenerasi Kalus Keladi Tikus (Typonium Flagelliforme. Lodd.) Secara in Vitro. Jurnal Penelitian Tanaman Industri, 13(4), 142-146, https://doi.org/10.21082/littri.v13n4.2007.%25p

Widowati, L., & Mudahar, H. (2009). Uji Aktivitas Ekstrak Etanol 50% Umbi Keladi Tikus (Typhonium Flagelliforme (Lood) Bl) terhadap Sel Kanker Payudara Mcf-7 in Vitro. Media Penelitian dan Pengembangan Kesehatan, 19(1), 9-14. Retrieved from https://media.neliti.com/media/publications/156140-ID-none.pdf

Author Biographies

Zulheri Noer, University of Medan Area

Prendy Jonringga Manik, University of Mataram

Saipul Sihotang, University of Mataram

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Copyright (c) 2023 Zulheri Noer, Prendy Jonringga Manik, Saipul Sihotang

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