Antioxidant Activity of Fruit Extract Powder Beans (Phaseolus vulgaris L.) Using DPPH Method

Authors

Rizki Nugrahani , Yayuk Andayani , Aliefman Hakim

DOI:

10.29303/jppipa.v6i2.409

Published:

2020-07-01

Issue:

Vol. 6 No. 2 (2020): July

Keywords:

antioxidant activity, Phaseolus vulgaris L., DPPH, IC50

Research Articles

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How to Cite

Nugrahani, R., Andayani, Y., & Hakim, A. (2020). Antioxidant Activity of Fruit Extract Powder Beans (Phaseolus vulgaris L.) Using DPPH Method. Jurnal Penelitian Pendidikan IPA, 6(2), 194–198. https://doi.org/10.29303/jppipa.v6i2.409
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Abstract

Free radical effect is one of many factors that can cause coronic desease, heart coronary desease, diabetes militus.  Beans plant (Phaseolus vulgaris L.) countant antioxidant compound. Antioxidants could inhibit oxidation reaction, that is cousing free radical compounds. The objective of this research was to determine the potential of bean extracts as an antioxidant. DPPH method was used to measure antioxidant activity, absorbances was measured using UV-Vis spectroscopy. Scrining phytochemical used to identified secondary metabolites in samples. The result showed IC50 1268.18; 2512; 1698.18 and 4442.75 μg/mL for all variety of stroge (less 1, 1, 2, 3 months) and IC50 control BHT was 1.744 μg/mL. Extract of bean powder sample classified as very weak antioxidant activity, variety of storage could effect to antioxidant activity. Phytochemical screening results obtained flavonoids, phenols, alkaloids, saponins, steroids and terpenoids present in the sample, while tannin are not included in the sample

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Author Biographies

Rizki Nugrahani, Program Studi Farmasi (D3), Fakultas Ilmu Kesehatan Universitas Nahdlatul Wathan Mataram

Yayuk Andayani, Program Studi Magister Pendidikan IPA, Program Pascasarjana Universitas Mataram

Aliefman Hakim, Program Studi Magister Pendidikan IPA, Program Pascasarjana Universitas Mataram

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