Multiplication of Dendrobium Sp Orchid Somatic Embries Using In-Vitro Concentrations of MgSO4 and Myo-Inositol in Murashige and Skoog Media

Authors

Jeni Santika , Elfi Indrawanis , Pebra Heriansyah

DOI:

10.29303/jppipa.v9i10.4425

Published:

2023-10-25

Issue:

Vol. 9 No. 10 (2023): October

Keywords:

Dendrobium sp, In-vitro, Media MS, MgSO4, Myo-inositol

Research Articles

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Santika, J. ., Indrawanis, E. ., & Heriansyah, P. . (2023). Multiplication of Dendrobium Sp Orchid Somatic Embries Using In-Vitro Concentrations of MgSO4 and Myo-Inositol in Murashige and Skoog Media . Jurnal Penelitian Pendidikan IPA, 9(10), 8016–8026. https://doi.org/10.29303/jppipa.v9i10.4425
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Abstract

Dendrobium Sp orchid is one type of orchid that occupies the top position in ornamental plants. This study aims to determine the administration of various concentrations of magnesium sulfate (MgSO4) and Myo-inositol to Dendrobium Sp orchid explants on Murashige and Skoog media. The design used in this study was a factorial complete randomized design (CRD) consisting of 2 treatment levels (M= MgSO4 and Y= Myo-inositol) with 3 replications. Namely: M0 (without MgSO4), M1 (MgSO4 350 mg/l ), M2 (MgSO4 370 mg/l), M3 (MgSO4 390 mg/l), and Y0 (Without Myo-inositol), Y1 (Myo-inositol 50 mg/l), Y2 (Myo-inositol 100 mg/l) , Y3 (Myo-inositol 150 mg/l). Based on the results of the study, giving various concentrations of magnesium sulfate (MgSO4) alone had a significant effect on all observed parameters, where the best treatment was found in M3 with an average number of shoots 4.04, shoot height 1.08 cm, number of leaves 7.89 fruit, number of roots 6.64 fruit and root length 1.55 cm in Dendrobium Sp. For the treatment of various Myo-inositol concentrations, a single significant effect on all observed parameters, where the best treatment was found in Y3 with an average number of shoots 4.03, shoot height 1.25 cm, number of leaves 7.81, number of roots 4.96 and root length 1.48 in Dendrobium Sp. In interaction, the administration of MgSO4 and Myo-inisitol had a significant effect on all observation parameters, the best treatment was M3Y3 (390 mg/l MgSO4 and 150 mg/l MS Myo-inisitol) namely the number of shoots 4.33, the height of the shoots 1.68 cm, the number of leaves is 8.89, the number of roots is 7.22 cm and the root length is 2.02 cm.

References

Akter, S., Nasiruddin, K. M., & Khaldun, A. B. M. (2007). Organogenesis of Dendrobium orchid using traditional media and organic extracts. Journal of Agriculture & Rural Development, 30–35. https://scholar.archive.org/work/reph2ewz25bqvnfrmhbo5ardyy/access/wayback/http://journals.sfu.ca:80/bd/index.php/JARD/article/download/1454/1419

Alamgir, A. N. M., & Alamgir, A. N. M. (2018). Vitamins, nutraceuticals, food additives, enzymes, anesthetic aids, and cosmetics. Therapeutic Use of Medicinal Plants and Their Extracts: Volume 2: Phytochemistry and Bioactive Compounds, 407–534. https://doi.org/10.1007/978-3-319-92387-1_5

Arobaya, A. Y. S., Zuhud, E. A. M., Siregar, I. Z., & Irawati, I. (2022). Diversity and distribution of epiphytic orchid Dendrobium section Spatulata on the host plants in the Cycloop Mountain Nature Reserve of Papua, Indonesia. Biodiversitas Journal of Biological Diversity, 23(4). https://doi.org/10.13057/biodiv/d230438

Bošnjak, D., Marković, M., Agić, D., Vinković, T., Tkalec Kojić, M., Ravnjak, B., & Stanisavljević, A. (2021). The influence of nutrient media modification on the morphological parameters in raspberry (Rubus idaeus L.) micropropagation in the liquid and semi-solid media. Poljoprivreda, 27(1), 22–29. https://doi.org/10.18047/poljo.27.1.3

Cardoso, J. C., Oliveira, M. E., Cardoso, F. de C. I., & others. (2019). Advances and challenges on the in vitro production of secondary metabolites from medicinal plants. Horticultura Brasileira, 37, 124–132. https://doi.org/10.1590/S0102-053620190201

Chhetri, V. S., Janes, M. E., King, J. M., Doerrler, W., & Adhikari, A. (2019). Effect of residual chlorine and organic acids on survival and attachment of Escherichia coli O157: H7 and Listeria monocytogenes on spinach leaves during storage. LWT, 105, 298–305. https://doi.org/10.1016/j.lwt.2019.02.019

Chin, C. K., Lee, Z. H., Mubbarakh, S. A., Antony, J. J. J., Chew, B. L., & Subramaniam, S. (2019). Effects of plant growth regulators and activated charcoal on somaclonal variations of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid. Biocatalysis and Agricultural Biotechnology, 22, 101426. https://doi.org/10.1016/j.bcab.2019.101426

Ciarkowska, A., Wojtaczka, P., Kkesy, J., & Ostrowski, M. (2023). Auxin homeostasis in maize (Zea mays) is regulated via 1-O-indole-3-acetyl-myo-inositol synthesis at early stages of seedling development and under abiotic stress. Planta, 257(1), 23. https://doi.org/10.1007/s00425-022-04058-z

Evans, S. A., Whigham, D. F., Hartvig, I., & McCormick, M. K. (2023). Hybridization in the Fringed Orchids: An Analysis of Species Boundaries in The Face of Gene Flow. Diversity, 15(3), 384. https://doi.org/10.3390/d15030384

Gunawan, L. W. (2021). Budi Daya Anggrek. Penebar Swadaya.

Hegeman, C. E., Good, L. L., & Grabau, E. A. (2001). Expression of D-myo-inositol-3-phosphate synthase in soybean. Implications for phytic acid biosynthesis. Plant Physiology, 125(4), 1941–1948. https://doi.org/10.1104/pp.125.4.1941

Hendaryono, I. D. P. S., & Wijayani, I. A. (1994). Teknik kultur jaringan, pengenalan dan petunjuk perbanyakan tanaman secara vegetatif-modern. Semarang: Kanisius.

Heriansyah, P., Sagiarti, T., & Rover, R. (2014). Pengaruh Pemberian Myoinositol Dan Arang Aktif Pada Media Sub Kultur Jaringan Tanaman Anggrek (Dendrobium SP). Jurnal Agroteknologi, 5(1), 9–16. https://doi.org/10.24014/ja.v5i1.1142

Hesami, M., Naderi, R., & Tohidfar, M. (2020). Introducing a hybrid artificial intelligence method for high-throughput modeling and optimizing plant tissue culture processes: the establishment of a new embryogenesis medium for chrysanthemum, as a case study. Applied Microbiology and Biotechnology, 104, 10249–10263. https://doi.org/10.1007/s00253-020-10978-1

Janakiram, T., & Baskaran, V. (2018). Commercialisation and conservation aspects of orchids. J. Orchid. Soc. India, 32, 55–61. http://orchidsocietyindia.org/wp-content/uploads/2019/09/COMMERCIALISATION_AND_CONSERVATION_ASPECTS_OF_ORCHIDS.pdf

Kabylbekova, B., Kovalchuk, I., Mukhitdinova, Z., Turdiyev, T., Kairova, G., Madiyeva, G., & Reed, B. M. (2020). Reduced major minerals and increased minor nutrients improve micropropagation in three apple cultivars. In Vitro Cellular & Developmental Biology-Plant, 56, 335–349. https://doi.org/10.1007/s11627-019-10019-1

Kasutjianingati, K., Koesparwanti, T. R., & Eliyatiningsih, E. (2022). Utilization of foliar fertilizer as an alternative medium for enlargement of Vanda orchid plantlets before acclimatization. IOP Conference Series: Earth and Environmental Science, 980(1), 12004. https://doi.org/10.1088/1755-1315/980/1/012004

Ketsa, S., & Warrington, I. J. (2023). The dendrobium orchid: Botany, horticulture, and utilization. Crop Science, 63(4). https://doi.org/10.1002/csc2.20952

Lone, S. M., Hussain, K., Malik, A., Magray, M., Hussain, S. M., Rashid, M., & Farwah, S. (2020). Plant propagation through tissue culture-a biotechnological intervention. International Journal of Current Microbiology and Applied Sciences, 9(7), 2176–2190. https://doi.org/10.20546/ijcmas.2020.907.254

Madzikane, O., Gebashe, F. C., & Amoo, S. O. (2022). Use of alternative components in cost-effective media for mass production of clonal plants. In Commercial Scale Tissue Culture for Horticulture and Plantation Crops (pp. 49–64). Springer. https://doi.org/10.1007/978-981-19-0055-6_3

Nguyen, H. T., Dinh, S. T., Ninh, T. T., Nong, H. T., Dang, T. T. T., Khuat, Q. V, Dang, A. T. P., Ly, M. T., Kirakosyan, R. N., & Kalashnikova, E. A. (2022). In vitro propagation of the Dendrobium anosmum Lindl. collected in Vietnam. Agronomy, 12(2), 324. https://doi.org/10.3390/agronomy12020324

Pammai, K., Al Muhdhar, M. H. I., SARI, M. S., SUEB, S., & YUHANNA, W. L. (2022). Inventory of orchid diversity in Merauke District, South Papua Province, Indonesia. Biodiversitas Journal of Biological Diversity, 23(11). https://doi.org/10.13057/biodiv/d231150

Phillips, G. C., & Garda, M. (2019). Plant tissue culture media and practices: an overview. In Vitro Cellular & Developmental Biology-Plant, 55, 242–257. https://doi.org/10.1007/s11627-019-09983-5

Poothong, S., & Reed, B. M. (2015). Increased CaCl 2, MgSO 4, and KH 2 PO 4 improve the growth of micropropagated red raspberries. In Vitro Cellular & Developmental Biology-Plant, 51, 648–658. https://doi.org/10.1007/s11627-015-9720-y

Puri, S., Heriansyah, P., & Nopsagiarti, T. (2022). Potassium Dihydrogen Phosphate (KH2PO4) and Kinetin Enhance The Growth of Dendrobium Sonia Somatic Embryos (Kalium Dihidrogen Fosfat (KH2PO4) dan Kinetin Meningkatkan Untuk Pertumbuhan Embrio Somatik Dendrobium Sonia). Jurnal Biologi Indonesia, 18(1), 41–50. https://doi.org/10.47349/jbi/18012022/41

Rahmi, N. A. (2022). Induksi embrio somatik anggrek ekor tupai (rhynchostylis gigantea (lindl.)) dengan perlakuan pencahayaan dan sitokinin [Fakultas Sains dan Teknologi UIN Syarif Hidayatullah Jakarta]. https://repository.uinjkt.ac.id/dspace/handle/123456789/64912

Ramage, C. M., & Williams, R. R. (2002). Mineral nutrition and plant morphogenesis. In Vitro Cellular & Developmental Biology-Plant, 38, 116–124. https://doi.org/10.1079/IVP2001269

Safitri, L. E. (2020). Aplikasi PGPR (Plant Growth Promoting Rhizobacteria) dan Gandasil B Dalam Meningkatkan Pertumbuhan dan Produksi Tanaman Cabai Rawit (Capsicum Frutescens L.) [Universitas Islam Riau]. https://repository.uir.ac.id/9905/

Sandra, I. E. (2004). Kultur Jaringan Anggrek Skala Rumah Tangga. Jakarta Selatan: AgroMedia.

Semiarti, E., Purwantoro, A., & Puspita Sari, I. (2020). Biotechnology approaches on characterization, mass propagation, and breeding of Indonesian orchids Dendrobium lineale (Rolfe.) and Vanda tricolor (Lindl.) with its phytochemistry. Orchids Phytochemistry, Biology and Horticulture: Fundamentals and Applications, 1–14. 10.1007/978-3-030-11257-8_12-1

Sharavdorj, K., Jang, Y., Byambadorj, S.-O., & Cho, J.-W. (2022). The effect of MgSO4 and CaSO4 on seedlings of forage crops under environmental stress. Plant Physiology Reports, 27(4), 702–716. https://doi.org/10.1007/s40502-022-00691-8

Sparjanbabu, D. S., Kumar, P. N., Krishna, M. S. R., Ramajayam, D., & Susanthi, B. (2019). Effect of activated charcoal, culture media and plant growth regulators on in vitro germination and development of elite dura oil palm (Elaeis guineensis Jacq.) zygotic embryos. http://krishi.icar.gov.in/jspui/handle/123456789/26733

Toma, R. S., Danial, G. H., & Habash, A. N. Y. (2012). In vitro morphogenetic response of apple (Malus domestica Borkh.) and pear (Pyrus communis L.) to the elevated levels of copper and myo-inositol. Acta Agrobotanica, 65(3). https://bibliotekanauki.pl/articles/28589.pdf

Wijaya, A. N., Artadana, I. B. M., Putra, S. E. D., & Hardjo, P. H. (2023). Effect of organic additives on regeneration of orchid hybrid (Dendrobium ‘Bertachong’X Dendrobium ‘Blackspider X Sutiknoi’). AIP Conference Proceedings, 2606(1). https://doi.org/10.1063/5.0118811

Wilks, J. M., Chen, F., Clark, B. C., & Schneegurt, M. A. (2019). Bacterial growth in saturated and eutectic solutions of magnesium sulphate and potassium chlorate with relevance to Mars and the ocean worlds. International Journal of Astrobiology, 18(6), 502–509. https://doi.org/10.1017/S1473550418000502

Xu, X. M., & Møller, S. G. (2004). AtNAP7 is a plastidic SufC-like ATP-binding cassette/ATPase essential for Arabidopsis embryogenesis. Proceedings of the National Academy of Sciences, 101(24), 9143–9148. https://doi.org/10.1073/pnas.0400799101

Yusnita, E. (2003). Kultur Jaringan: Cara memperbanyak tanaman secara efisien. Jakarta: Agro Media Pustaka. Jakarta.

Author Biographies

Jeni Santika, Universitas Islam Kuantan Singingi Teluk Kuantan

 

 

Elfi Indrawanis, Universitas Islam Kuantan Singingi Teluk Kuantan

 

 

Pebra Heriansyah, Universitas Islam Kuantan Singingi, Teluk Kuantan. Jln. Gatot Subroto KM 7 Jake Telp : 082383267805 Teluk Kuantan, Riau

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