The Benefits of Nori Fruit Extract (Morinda Citrifolia Linn) in Increasing the Storage of Spermatozoa Y Bali Cows Resulting from Sexing at a Temperature of 5oC

Authors

Sri Firmiaty , Muhammad Idrus , Bestfy Anitasari , Hamsina Hamsina

DOI:

10.29303/jppipa.v9i9.4785

Published:

2023-09-25

Issue:

Vol. 9 No. 9 (2023): September

Keywords:

Bali cattle, Morinda citrifolia extract, Sexing sperm, Y chromosome

Research Articles

Downloads

How to Cite

Firmiaty, S. ., Idrus, M. ., Anitasari, B. ., & Hamsina, H. (2023). The Benefits of Nori Fruit Extract (Morinda Citrifolia Linn) in Increasing the Storage of Spermatozoa Y Bali Cows Resulting from Sexing at a Temperature of 5oC. Jurnal Penelitian Pendidikan IPA, 9(9), 7073–7078. https://doi.org/10.29303/jppipa.v9i9.4785
Crossref
Scopus
Google Scholar
Europe PMC

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

The sperm sexing technique is the separation of spermatozoa carrying the X chromosome and the Y chromosome, which allows farmers to choose male births for beef cattle businesses. However, the process of centrifugation and sperm preservation can cause oxidative stress which gives rise to free radicals. Therefore, an antioxidant ingredient is needed in the diluent, including noni fruit extract which contains lots of flavonoids. This research aims to analyze the addition of noni fruit extract in a diluent to improve the quality of Y-chromosome spermatozoa at a storage temperature of 5oC. The results of the study showed the results of analysis of the progressive motility of Y-chromosome spermatozoa, showing a decrease in progressive motility, on D7 and D-9 there was a difference between control vs P1 and P2 (P<.05) respectively 37.63% vs 49.96% vs 48.56%, while on day 11 showed a very real difference between P0 vs P1 vs P2 respectively 24.32% vs 36.77% vs 40.87%. The addition of 3% and 5% noni extract can improve the quality of Y spermatozoa stored at 5oC. This is because noni extract contains amino acids, ascorbic acid, and flavonoids as antioxidants so that it is able to prevent free radicals, scavengers, and damage to the spermatozoa plasma membrane can be prevented. The addition of 5% noni fruit extract in the diluent to improve the quality of Y-chromosome spermatozoa at a storage temperature of 5oC for up to 11 days.

References

Disnakkeswan Provinsi Jawa Tengah. (2023). Larangan pemotongan ternak ruminansia betina produktif pada hari raya idul adha tahun 2020.

Garner, D. L. J., & Seidel, G. E. (2000). Sexing bull sperm. In P. J. Chenoweth (Ed.), Topics in Bull Fertility (pp. 10–12). International Veterinary Information Service. Retrieved from https://www.ivis.org/library/topicsbull-fertility/sexing-bull-sperm.

Gunawan, M. (2015). Aplikasi inseminasi buatan dengan sperma sexing dalam meningkatkan produktivitas sapi di peternakan rakyat. Pros Sem Nas Masy Biodiv Indon, 1(1), 93–96. https://doi.org/10.13057/psnmbi/m010115

Haris, F. Z., Soepri Ondho, Y., & Samsudewa, D. (2020). Effect of vitamin E addition to frozen Simmental bull semen extender on post-thawing quality. E3S Web of Conferences, 142(6), 02002. https://doi.org/10.1051/e3sconf/202014202002

Herdis, Toelihere, M. R., Supriatna, I., Purwantara, B., & Adikara, R. (2005). Optimalisasi Kualitas Semen Cair Domba Garut (Ovis Aries) Melalui Penambahan Maltosa ke dalam Pengencer Semen Tris Kuning Telur. Jurnal Media Kedokteran Hewan, 21(2), 88–93. Retrieved from https://journal.unair.ac.id/download-fullpapers-MKH-21-2-21.pdf

Hossain, M. A., Salehuddin, S. M., & Ismail, Z. (2006). Rosmarinic acid and methyl rosmarinate from Orthosiphon stamineus. Benth. J Bang Acad Scis, 30(2), 167–171. Retrieved from https://www.researchgate.net/publication/312911344_Rosmarinic_acid_and_methyl_rosmarinate_from_Orthosiphon_stamineus_Benth.

Lukman, H., Rodiah, Yuliani, E., Zaenuri, L. A., & Sumadiasa, I. W. L. (2020). Penerapan Tehnologi Inseminasi Buatan Menggunakan Sperma Sexing pada Ternak Sapi di Kecamatan Lingsar Kabupaten Lombok Barat. Abdi Insani, 7(2), 121–125. https://doi.org/10.29303/abdiinsani.v7i2.292

Michael, A., Alexopoulos, C., Pontiki, E., Hadjipavlou-Litina, D., Saratsis, P., Ververidis, H., & Boscos, C. (2010). Effect of N-acetyl-L-cysteine Supplementation in Semen Extenders on Semen Quality and Reactive Oxygen Species of Chilled Canine Spermatozoa. Reproduction in Domestic Animals, 45(2), 201–207. https://doi.org/10.1111/j.1439-0531.2008.01202.x

Nugraha, C. D., Herwijanti, E., Novianti, I., Furqon, A., Septian, W. A., Busono, W., & Suyadi, S. (2019). Correlations between age of Bali bull and semen production at National Artificial Insemination Center, Singosari-Indonesia. Journal of the Indonesian Tropical Animal Agriculture, 44(3), 258. https://doi.org/10.14710/jitaa.44.3.258-265

Purwoistri, R. F., Susilawati, T., & Rahayu, S. (2013). Membran Spermatozoa Hasil Seksing Gradien Albumin Berpengencer Andromed dan Cauda Epididymal Plasma-2 Ditambahkan Kuning. Jurnal Veteriner, 14(3), 371–378. Retrieved from https://ojs.unud.ac.id/index.php/jvet/article/view/7275.

Quan, G. B., Wu, G. Q., Wang, Y. J., Li, D. J., Ma, Y., & Hong, Q. H. (2016). Effects of the Tris, Tes, or skim milk based extender on in vitro parameters of ram spermatozoa during liquid storage. Small Ruminant Research, 134, 14–21. https://doi.org/10.1016/j.smallrumres.2015.11.008

Ratnawati, D., Isnaini, N., & Susilawati, T. (2018). Character motility of liquid semen on Ongole crossbreed (PO), Bali and Madura bulls with diluent CEP-2at cold storage. Asian Journal of Microbiology, Biotechnology and Environmental Sciences, 20(1), 21–28. Retrieved from https://rb.gy/6ucbr

Rizal, M. (2009). Daya hidup spermatozoa epidididmis sapi bali yang dipreservasi pada suhu 3-5oC dalam pengencer tris dngan konsentrasi laktosa yang berbeda. Jurnal Ilmu Ternak Dan Veteriner, 14(2), 142–149. Retrieved from https://core.ac.uk/download/pdf/236132729.pdf.

Rohman, A., & Riyanto, S. (2017). Aktivitas Antioksidan Ekstrak Buah Mengkudu (Morinda citrifolia, L). AgriTECH, 25(3), 131. https://doi.org/10.22146/agritech.13347

Savitria, F. K., Suharyatib, S., & Siswanto. (2014). Kualitas semen beku sapi Bali dengan penambahan Berbagai dosis vitamin C pada bahan pengencer skim Kuning telur. Jurnal Ilmiah Peternakan Terpadu, 2(3), 30–36. https://doi.org/10.23960/jipt.v2i3.p

Sholikah, N., Isnaini, N., Puspita Anugra Yekti, A., & Susilawati, T. (2016). Pengaruh penggantian Bovine Serum Albumin (BSA) dengan putih telur pada pengencer CEP-2 terhadap kualitas semen sapi Peranakan Ongole pada suhu penyimpanan 3-5oC. Jurnal Ilmu-Ilmu Peternakan, 26(1), 7–15. https://doi.org/10.21776/ub.jiip.2016.026.01.2

Simamora, A. (2009). Flavonoid dalam Apel dan Aktivitas Antioksidannya. Jurnal Kedokteran, 15(40), 1–16. https://doi.org/10.36452/jkdoktmeditek.v15i40.192.

Simanjuntak, K. (2012). Peran Antioksidan Flavonoid dalam Meningkatkan Kesehatan. Bina Widya, 23(3), 135–140. Retrieved from http://library.upnvj.ac.id//index.php?p=show_detail&id=7825.

Sjabana, D., & Ramadhani, R. B. (2002). Pesona Tradisional dan Ilmiah, Mengkudu, Morinda Citrifolia, Seri referensi Herbal. Penerbit Salemba Medika. https://doi.org/http://library.poltekkespalembang.ac.id/ucs/index.php?p=show_detail&id=1673.

Sudjana, N. (2005). Metode statistika. Bandung: Tarsito.

Sugiarto, N., Susilawati, T., & Wahyuningsih S. (2014). Kualitas semen cair sapi Limousin selama pendinginan menggunakan pengencer CEP-2 dengan penambahan berbagai konsentrasi sari kedelai. Jurnal Ternak Tropika, 15(1), 51–57. Retrieved from https://ternaktropika.ub.ac.id/index.php/tropika/article/view/194.

Susilawati, T. (2013). Pedoman Inseminasi Buatan pada Ternak. UB Press. Retrieved from http://digilib.ub.ac.id/opac/detail-opac?id=11915.

Susilawati, T. (2014). In Sexing Spermatozoa. UB Press. Retrieved from http://elib.universitasbosowa.ac.id//index.php?p=show_detail&id=26292.

Susilawati, T., Ratnawati, D., Isnaini, N., Kuswati, & Yekti, A. P. (2018). Character Of Liquid Semen Motility In Various Diluents On Balinese Cattle During Cold Storage. Asian Journal of Microbiology, Biotechnology and Environmental Sciences, 20(1), 166–172. Retrieved from http://www.envirobiotechjournals.com/article_abstract.php?aid=8497&iid=244&jid=1

Susilawati, T., & Wahyuningsih, S. (2013). Spermatozoa viability of Limousin cattle preserved with water jacket and free water jacket method. Jurnal Veteriner, 14(3), 379–386. Retrieved from https://www.cabdirect.org/cabdirect/abstract/20133418979

Verstegen, J., Iguer-Ouada, M., & Onclin, K. (2002). Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology, 57(1), 149–179. https://doi.org/10.1016/S0093-691X(01)00664-1

Wadood, F., Aleem, M., Ijaz, A., Mughal, D. H., & Gondal, M. A. (2022). Comparative efficacy of coconut water diluent with different semen extenders for cryopreservation of Nili Ravi buffalo bull semen. Buffalo Bulletin, 41(3), 363. https://doi.org/10.56825/bufbu.2022.4132994

Yekti, A. P. A., Kusumawati, E. D., Kuswati, K., Ridhowi, A., Sudarwati, H., Isnaini, N., & Susilawati, T. (2018). Succesfull of Artificial Insemination by Using Chilled Semen on Brahman Cross Cows. Proceedings of the 1st International Conference in One Health (ICOH 2017), 5(1), 221–226. https://doi.org/10.2991/icoh-17.2018.43

Yuliani, E., & Lukman, H. Y. (2013). Application of Sperm Sexing Base don Antioxidants to the Quality, Integrity of the Membrane and Fertility of Bali Cattle. Seminar Nasional Tehnologi Peternakan Dan Veteriner, 23–29. Retrieved from http://eprints.unram.ac.id/id/eprint/37884.

Author Biographies

Sri Firmiaty, Bosowa University

Muhammad Idrus, Bosowa University

Bestfy Anitasari, Institut Kesehatan dan Bisnis Kurnia Jaya Persada

Hamsina Hamsina, Universitas Bosowa

License

Copyright (c) 2023 Sri Firmiaty, Muhammad Idrus, Bestfy Anitasari, Hamsina Hamsina

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).