The Effect of Additional Chili (Capsicum annum) and Sugar on Total Lactic Acid Bacteria (LAB) from Sauerkraut

Authors

Vauzia , Resti Fevria , Indiastri P Monica , Moralita Chatri , Afifatul Achyar , Edwin

DOI:

10.29303/jppipa.v9iSpecialIssue.4811

Published:

2023-12-25

Issue:

Vol. 9 No. SpecialIssue (2023): UNRAM journals and research based on science education, science applications towards a golden Indonesia 2045

Keywords:

Chili, Lactic Acid Bacteria, Sauerkraut, Sugar

Research Articles

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Vauzia, V., Fevria, R. ., Monica , I. P., Chatri, M. ., Achyar, A. ., & Edwin, E. (2023). The Effect of Additional Chili (Capsicum annum) and Sugar on Total Lactic Acid Bacteria (LAB) from Sauerkraut. Jurnal Penelitian Pendidikan IPA, 9(SpecialIssue), 1165–1171. https://doi.org/10.29303/jppipa.v9iSpecialIssue.4811

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Abstract

Sauerkraut is a typical German food made from thinly sliced cabbage. Sauerkraut is a fermeted product that can give a distinctive taste. Sugar is an ingredient that can be added in, that sugar can trigger the growth of lactic acid bacteria. Indonesian people like food with a spicy sensation from chilies, chillies here contain vitamin C which functions as an antioxidant that is good for the body and is able to increase endurance. This study aims to calculate the total lactic acid bacteria contained in sauerkraut with the addition of chilies and sugar. Dilution of 1 ml sample into 9 ml physiological NaCl solution from 10 -1 to 10 -8 dilutions was carried out, then inoculated into MRS Agar using the pour plate method. Incubated in room temperature for 48 hours. The growing colonies of lactic acid bacteria were then observed based on colony morphology and counting the total growing lactic acid bacteria. The results of the research can be concluded that there was an increase in the number of lactic acid bacteria in sauerkraut with the addition of chili and 10% granulated sugar with a bacterial density average is 88.667 x 108 CFU/mL.

References

Aljahani, A. H. (2020). Microbiological and Physicochemical Quality of Vegetable Pickles. Journal of the Saudi Society of Agricultural Sciences, 19(6), 415-421.

An, F., Sun, H., Wu, J., Zhao, C., Li, T., Huang, H., & Wu, R. (2021). Investigating the Core Microbiota and Its Influencing Factors in Traditional Chinese Pickles. Food Research International, 147, 110543. https://doi.org/10.1016/j.foodres.2021.110543

Breidt, F., McFeeters, R. F., Perez-Diaz, I., Lee, C. H., Doyle, M. P., & Buchanan, R. L. (2013). Food Microbiology: Fundamentals and Frontiers. Fermented Vegetables, 841-855.

Cai, W., Tang, F., Wang, Y., Zhang, Z., Xue, Y., Zhao, X., & Shan, C. (2021). Bacterial Diversity and Flavor Profile of Zha-Chili, a Traditional Fermented Food in China. Food Research International, 141, 110112. https://doi.org/

1016/j.foodres.2021.110112

Di Cagno, R., Coda, R., De Angelis, M., & Gobbetti, M. (2013). Exploitation of Vegetables and Fruits Through Lactic Acid Fermentation. Food Microbiology, 33(1), 1-10. https://doi.org/10.1016/

j.fm.2012.09.003

Fan, L., & Hansen, L. T. (2012). Fermentation and Biopreservation of Plant-Based Foods with Lactic Acid Bacteria. In Handbook of Plant-Based Fermented Food and Beverage Technology (pp. 56-69). CRC Press.

Fevria, R., & Hartanto, I. (2019). Isolation and Characterization of Lactic Acid Bacteria (Lactobacillus sp) from Sauerkraut with the addition of Cayenne Pepper. Bioscience, 3(2), 169–175.

Fevria, R., & Hartanto, I. (2020). Isolation and Characterization of Lactic Acid Bacteria (Lactobacillus sp.) from Sauerkraut. International Conference on Biology, Sciences and Education (ICoBioSE 2019), 74–77.

Fevria, R., Chatri, M., & Achyar, A. (2020). Pengaruh Penambahan Gula terhadap Waktu Fermentasi Sauerkraut dari Kol (Brassica Oleracea L.). Symbiotic: Journal of Biological Education and Science, 1(2), 70–75.

Hassan, Q. U., & Sarfraz, R. A. (2018). Effect of Different Nutraceuticals on Phytochemical and Mineral Composition as Well as Medicinal Properties of Home Made Mixed Vegetable Pickles. Food Biology, 7(0), 24-27. https://doi.org/10.25081/fb.2018.v7.3666

Hassan, S. S., bt Abd Malek, R., Atim, A., Jikan, S. S., & Mohd Fuzi, S. F. Z. (2015). Effects of Different Carbon Sources for High Level Lactic Acid Production by Lactobacillus casei. Applied Mechanics and Materials, 695, 220-223.

Hien, T. T., Truc, T. T., & Muoi, N. V. (2022). Effect of Salt Concentration and pH Value on the Lactic Fermentation Process of Kohlrabi (Brassica oleracea L.). Research and Innovation in Food Science and Technology, 10(4), 399-412. https://doi.org/

22101/JRIFST.2021.295188.1256

Jeong, S. H., Lee, H. J., Jung, J. Y., Lee, S. H., Seo, H. Y., Park, W. S., & Jeon, C. O. (2013). Effects of Red Pepper Powder on Microbial Communities and Metabolites During Kimchi Fermentation. International journal of food microbiology, 160(3), 252-259. https://doi.org/10.1016/j.ijfoodmicro.2012.

015

Koswara, S. (2013). Teknologi Fermentasi Sayuran [Vegetable Fermentation Technology]. Retrieved from http://tekpan.unimus.ac.id/

Krishnan, V., Seervi, P., Bhati, A., Singhal, H., Sushil, I. S., & Prazapati, P. (2014). Study of Traditional Methods of Food Preservation, Its Scientific Understanding and Technological Intervention. Indian Institute of Technology, Mandi (India), ISTP (Group-7) final report.

Lahtinen, S., Ouwehand, A. C., Salminen, S., & von Wright, A. (Eds.). (2011). Lactic Acid Bacteria: Microbiological and Functional Aspects. Crc Press.

Mahrus, M., Al Idrus, A., Syukur, A., & Zulkifli, L. (2023). The Effects of Pineapple Fruit Extracts (Ananas comosus) on the Quality of Chemical and Microbiological-Rabbitfish (Siganus spp.) Sauce Products. Jurnal Penelitian Pendidikan IPA, 9(12), 11000-11009. https://doi.org/10.2903/jppipa.v9i2.

Masood, M. I., Qadir, M. I., Shirazi, J. H., & Khan, I. U. (2011). Beneficial Effects of Lactic Acid Bacteria on Human Beings. Critical Reviews in Microbiology, 37(1), 91–98. https://doi.org/10.3109/1040841X.

536522

Mohamed, W. F., Khalil, K. M., Shady, H. M. A., & Sayed, H. A. E. (2016). Activity Range Determination and Optimization of Various Environmental Conditions for Bacteriocin Production by Lactobacillus acidophilus and Lactobacillus plantarum. The Egyptian Journal of Experimental Biology (Botany), 12, 53–66.

Monika, M., Savitri, S., Kumar, V., Kumari, A., Angmo, K., & Bhalla, T. C. (2017). Isolation and Characterization of Lactic Acid Bacteria from Traditional Pickles of Himachal Pradesh, India. Journal of Food Science and Technology, 54, 1945-1952. https://doi.org/10.1007/s13197-017-2629-1

Normayanti, N., Sari, D. K., & Khairina, R. (2020). Effects of Addition Chili Ground to The Characteristics of Bekasam from Cork Fish (Channa striata) During Fermentation. International Journal of Advance Tropical Food, 2(1). http://dx.doi.org/10.26877/ijatf.v2i1.6172

Patel, M. (2019). Effect of Physical and Microbiological Parameters on Ready Made Pickles: A Review. International Journal of Food Science and Nutrition Engineering, 9(2), 31-36. https://doi.org/10.5923/

j.food.20190902.01

Pato, U., Johan, V. S., Fitriani, S., & Zelviani, P. F. (2019). Karakteristik Asinan Kubis yang Dibuat dengan Penambahan Isolat Lactobacillus plantarum 1. Jurnal Teknologi Pangan, 13(1), 39–47.

Peñas, E., Martinez-Villaluenga, C., & Frias, J. (2017). Sauerkraut. Fermented Foods in Health and Disease Prevention, 557–576. https://doi.org/10.1016/b978

-0-12-802309-9.00024-8

Petrut, S., Rusu, E., Tudorache, I. S., Pelinescu, D., Sarbu, I., Stoica, I., & Vassu, T. (2019). Influence of Various Carbon Sources on Growth and Biomass Accumulation of Some Lactic Acid Bacteria Strains. Pharmaceuticals, 4, 5.

Rahayu, H. M., & Setiadi, A. E. (2023). Isolation and Characterization of Indigenous Lactic Acid Bacteria from Pakatikng Rape, Dayak’s Traditional Fermented Food. Jurnal Penelitian Pendidikan IPA, 9(2), 920-925. https://doi.org/10.2903/jppipa.

v9i2.2801

Rahmah, A., Izzati, M., & Parman, S. (2014). Pengaruh Pupuk Organik Cair Berbahan Dasar Limbah Sawi Putih (Brassica Chinensis L.) terhadap Pertumbuhan Tanaman Jagung Manis (Zea Mays L. Var. Saccharata). Anatomi Fisiologi, 22(1), 65–71.

Rosmainar, L., Ningsih, W., Ayu, N. P., & Nanda, H. (2018). Penentuan Kadar Vitamin C Beberapa Jenis Cabai (Capsicum sp.) dengan Spektrofotometri UV-VIS. Jurnal Kimia Riset, 3(1), 1–5.

Saeed, A. H., & Salam, A. I. (2013). Current Limitations and Challenges with Lactic Acid Bacteria: A Review. Food and Nutrition Sciences, 2013. https://doi.org/10.4236/fns.2013.411A010

Sawada, K., Koyano, H., Yamamoto, N., & Yamada, T. (2021). The Effects of Vegetable Pickling Conditions on the Dynamics of Microbiota and Metabolites. PeerJ, 9, e11123. https://doi.org/

7717/peerj.11123

Sayin, F. K., & Alkan, S. B. (2015). The Effect of Pickling on Total Phenolic Contents and Antioxidant Activity of 10 Vegetables. Food and Health, 1(3), 135-141. https://doi.org/10.3153/JFHS15013

Schneider, M. (1988). Microbiology of Sauerkraut Fermented in Small Ready-to-Sell Containers (Doctoral dissertation. Hohenheim University, Stuttgart, Germany).

Singh, A., Mani, A., Dutta, P., & Murmu, D. (2017). Changes in Lactic Acid Bacteria and Total Bacterial Population in Sodium Substituted Mix-Vegetable Pickle Under Ambient Storage Conditions. International Journal of Chemical Studies, 5, 261-264.

Tsaaqifah, H., Fahrurrozi, F., & Meryandini, A. (2023). Selection of Lactic Acid Bacteria as Starter Culture for Cocoa Fermentation (Theobroma Cacao L.). Jurnal Penelitian Pendidikan IPA, 9(2), 825-831. https://doi.org/10.2903/jppipa.v9i2.3045

Utama, C. S., Zuprizal, Z., Hanim, C., & Wihandoyo, W. (2018). Isolasi dan Identifikasi Bakteri Asam Laktat Selulolitik yang Berasal dari Jus Kubis Terfermentasi. Jurnal Aplikasi Teknologi Pangan, 7(1).

Wiander, B., & Ryhänen, E. L. (2005). Laboratory and Large-Scale Fermentation of White Cabbage into Sauerkraut and Sauerkraut Juice by Using Starters in Combination with Mineral Salt with a Low NaCl Content. European Food Research and Technology, 220, 191-195. https://doi.org/

1007/s00217-004-1080-5

Zhang, S., Xiao, Y., Jiang, Y., Wang, T., Cai, S., Hu, X., & Yi, J. (2022). Effects of Brines and Containers on Flavor Production of Chinese Pickled Chili Pepper (Capsicum frutescens L.) During Natural Fermentation. Foods, 12(1), 101. https://doi.org/

3390/foods12010101

Zulfahmi, Z., & Nirmagustina, D. E. (2012). Pengaruh Sukrosa terhadap Kandungan Total Fenol Minuman Rempah Tradisional (Minuman Secang). Jurnal Penelitian Pertanian Terapan, 12(2)

Author Biographies

Vauzia, Universitas Negeri Padang

Resti Fevria, Universitas Negeri Padang

Indiastri P Monica , Universitas Negeri Padang

Moralita Chatri, Universitas Negeri Padang

Afifatul Achyar, Universitas Negeri Padang

Edwin, Andalas University

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Copyright (c) 2023 Vauzia, Resti Fevria, Indiastri P Monica , Moralita Chatri, Afifatul Achyar, Edwin

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