Antioxidant and Antimalarial Potential of Methanolic Extract from Leaves of Titi Tree (Alstonia sp)

Authors

Maria Nindatu , Matha Kaihena , Adriana Hieriej , Amos Killay , Hery Jotlely

DOI:

10.29303/jppipa.v9i10.4865

Published:

2023-10-25

Issue:

Vol. 9 No. 10 (2023): October

Keywords:

Alstonia sp. leaves, Antimalarial, Antioxidants

Research Articles

Downloads

How to Cite

Nindatu, M., Kaihena, M. ., Hieriej, A. ., Killay, A. ., & Jotlely, H. (2023). Antioxidant and Antimalarial Potential of Methanolic Extract from Leaves of Titi Tree (Alstonia sp). Jurnal Penelitian Pendidikan IPA, 9(10), 8918–8924. https://doi.org/10.29303/jppipa.v9i10.4865
Crossref
Scopus
Google Scholar
Europe PMC

Downloads

Metrics

PDF views
125
Oct 25 '23Oct 28 '23Oct 31 '23Nov 01 '23Nov 04 '23Nov 07 '23Nov 10 '23Nov 13 '23Nov 16 '23Nov 19 '23Nov 22 '234.0
|

Abstract

Alstonia sp leaves extract has previously been reported as a potential antimalarial drug as traditional. This study aims to analyze the antimalarial activity and determine the antioxidant potential of titi tree leaves (Alstonia sp). The method used in this research is a modified method of "4 Days Suppressive Test" which was originally described by Peter with different doses (0.55 g/kg, 0.65 g/kg and 0.75 g/kg body weight) of methanol extract of leaves of Titi tree (Alstonia sp) was administered orally to albino mice. Antioxidants were analyzed using a spectrophotometer. The Leaves of Alstonia sp has antimalarial activity and contains potential antioxidant activity with an IC50 value of 70.12 ppm for leaves (strong category). The dose of 0.75 g has a better effect compared to the other effects because it lowers the level of parasitemia more strongly. So that the leaves of Alstonia sp. pressured to be developed as a nutraceutical.

References

Abu, N. E., Ezeomeke, S. I., Azegba, P., & Davidson, G. I. (2016). Phytochemical, nutritional and anti-nutritional properties of leaves, stems bark and roots of trees used in popular medicine for the treatment of malaria in South Eastern Nigeria. Journal of Medicinal Plants Research, 10(38), 662–668. https://doi.org/10.5897/JMPR2016.6249

Adotey, J. P. K., Adukpo, G. E., Opoku Boahen, Y., & Armah, F. A. (2012). A Review of the Ethnobotany and Pharmacological Importance of Alstonia boonei De Wild (Apocynaceae). ISRN Pharmacology, 2012, 1–9. https://doi.org/10.5402/2012/587160

Almatsier, S. (2009). Basic Principles of Nutrition Science. PT Gramedia Pustaka Utama.

Amole, O. O., & Ilori, O. O. (2010). Antimicrobial Activity of the Aqueous and Ethanolic Extracts of the Stem Bark of Alstonia Boonei. International Journal of Phytopharmacology, 1(2), 119–123. Retrieved from https://citeseerx.ist.psu.edu/document?repid=rep1&type=pdf&doi=0434f549ac29696183e60082f85d0ab5b8bbba7c

Antony, M., Menon, D. B., Joel, J., Lipin, D., Arun, K., & Thankamani, V. (2011). Phytochemical analysis and antioxidant activity of Alstonia scholaris. Pharmacognosy Journal, 3(26), 13–18. https://doi.org/10.5530/pj.2011.26.3

Atanu, F. O., Idih, F. M., Nwonuma, C. O., Hetta, H. F., Alamery, S., & El-Saber Batiha, G. (2021). Evaluation of Antimalarial Potential of Extracts from Alstonia boonei and Carica papaya in Plasmodium berghei-Infected Mice. Evidence-Based Complementary and Alternative Medicine, 2021, 1–11. https://doi.org/10.1155/2021/2599191

Choudhary, M., Rani, S., Sharma, P., Choudhary, N., & Budhwaar, V. (2017). Anti-fertility and abortifacient potential of hydroalcoholic leaves extract of Alstonia scholaris in female rats: An ethnomedicine used by Papua women in New Guinea. Bulletin of Faculty of Pharmacy, Cairo University, 55(1), 123–127. https://doi.org/10.1016/j.bfopcu.2017.01.005

Cimanga, R. K., Nsaka, S. L., Tshodi, M. E., Mbamu, B. M., Kikweta, C. M., Makila, F. B.-M., Cos, P., Maes, L., Vlietinck, A. J., Exarchou, V., Tuenter, E., & Pieters, L. (2019). In vitro and in vivo antiplasmodial activity of extracts and isolated constituents of Alstonia congensis root bark. Journal of Ethnopharmacology, 242, 111736. https://doi.org/10.1016/j.jep.2019.02.019

Dhruti, M., Bhavika, P., & Meonis, P. (2016). Studies on phytochemical constituents and antioxidant activity of Alstonia scholaris. Int. J. of Life Sciences, 4(4), 529–538. Retrieved from https://rb.gy/1u3iyz

Djeridane, A., Yousfi, M., Nadjemi, B., Boutassouna, D., Stocker, P., & Vidal, N. (2006). Antioxidant activity of some algerian medicinal plants extracts containing phenolic compounds. Food Chemistry, 97(4), 654–660. https://doi.org/10.1016/j.foodchem.2005.04.028

Fagbohun, E. D., & Bamikole, A. M. (2019). Antifungal Effects of Methanolic Extract of Stem Bark of Bridelia ferruginea Benth. Leaves of Aloe vera L. and Stem Bark of Alstonia boonei De Wild. Microbiology Research Journal International, 27(2), 1–11. https://doi.org/10.9734/mrji/2019/v27i230094

Gbadamosi, I., Moody, J., & Lawal, A. (2011). Phytochemical screening and proximate analysis of eight ethnobotanicals used as antimalaria remedies in Ibadan, Nigeria. Journal of Applied Biosciences, 44, 2967–2971. Retrieved from https://www.academia.edu/download/48087438/My_Publication.pdf

Gnoatto, S. C. B., Dassonville-Klimpt, A., Da Nascimento, S., Galéra, P., Boumediene, K., Gosmann, G., Sonnet, P., & Moslemi, S. (2008). Evaluation of ursolic acid isolated from Ilex paraguariensis and derivatives on aromatase inhibition. European Journal of Medicinal Chemistry, 43(9), 1865–1877. https://doi.org/10.1016/j.ejmech.2007.11.021

Havlin, J. L., Beaton, J. D., SL, T., & Nelson, W. L. (2017). Soil Fertility and Fertilizers: An Introduction to Nutrient Management (7th ed.). Pearson Educational, Inc. Retrieved from https://handoutset.com/wp-content/uploads/2022/05/

Hidayat, A. A. A. (2014). Midwifery Research Methods and Data Analysis Techniques. Salemba Medika. Retrieved from http://opac.perpusnas.go.id/DetailOpac.aspx?id=32259

Hussain, A., Zaman, M. K., & Ramteke, A. (2013). Preliminary Phytochemical Screening and Proximate Analysis of the trunk bark of Alstonia scholaris (L.) R.Br. Journal of Pharmacognosy and Phytochemistry, 1(5), 13–17. Retrieved from https://www.phytojournal.com/archives/2013/vol1issue5/PartA/3.1.pdf

Idowu, E. T., Ajaegbu, H. C., Omotayo, A. I., Aina, O. O., & Otubanjo, O. A. (2016). In vivo anti-plasmodial activities and toxic impacts of lime extract of a combination of Picralima nitida, Alstonia boonei and Gongronema latifolium in mice infected with Chloroquine-sensitive Plasmodium berghei. African Health Sciences, 15(4), 1262. https://doi.org/10.4314/ahs.v15i4.27

Intan, P. R., Winarno, M. W., & Prihartini, N. (2016). Efek Effects of Pulai (Alstonia scholaris) and Meniran (Phyllanthus niruri) Bark Extract Mixture on Mice (Swiss webster) Infected with Plasmodium berghei. Jurnal Kefarmasian Indonesia, 6(2), 79–88. Retrieved from https://www.neliti.com/id/publications/105891/

Iyiola, O. A., Tijani, A. Y., & Lateef, K. M. (2011). Antimalarial Activity of Ethanolic Stem Bark Extract of Alstonia boonei in Mice. Asian Journal of Biological Sciences, 4(3), 235–243. https://doi.org/10.3923/ajbs.2011.235.243

Jong-Anurakkun, N., Bhandari, M. R., & Kawabata, J. (2007). α-Glucosidase inhibitors from Devil tree (Alstonia scholaris). Food Chemistry, 103(4), 1319–1323. https://doi.org/10.1016/j.foodchem.2006.10.043

Khyade, M. S., & Vaikos, N. P. (2014). Wrightia tinctoria R. Br.-a review on its ethnobotany, pharmacognosy and pharmacological profile. Journal of Coastal Life Medicine, 2(10), 826–840. https://doi.org/10.12980/JCLM.2.2014C1221

Loncaric, C., Ernst, M. D., & Torlak, E. (2018). Generalized data structure synthesis. Proceedings of the 40th International Conference on Software Engineering, 958–968. https://doi.org/10.1145/3180155.3180211

Olafadehan, O. A., Oluwafemi, R. A., & Alagbe, J. O. (2020). Performance , haemato-biochemical parameters of broiler chicks administered Rolfe (Daniellia oliveri) leaf extract as an antibiotic alternative. Drug Discovery, 14(33), 135–145. Retrieved from http://www.discoveryjournals.org/drugdiscovery/current_issue/2020/v14/n33

Otuu, C. A., Obiezue, R. N. N., Okoye, C. I., Omalu, I. C. J., Udeh, E. O., Eke, S. S., Hassan, S. C., Ekuma, I. C., Yamman, H. U., Otuu, A. Q. A., & Okafor, F. C. (2021). Determination of Antimalarial Property, Chemical Constituents and Toxicity Level of Alstonia Boonei Aqueous Stem Bark Extract in Plasmodium Berghei Infected Mice. International Journal of Innovative Research and Development, 10(2), 192–198. https://doi.org/10.24940/ijird/2021/v10/i2/FEB21047

Peter, M. (2004). The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 50(June 2003), 211–219. Retrieved from http://rdo.psu.ac.th/sjstweb/index.php

Rahim, N. A., Mail, M. H., Muhamad, M., Sapuan, S., SMN Mydin, R. B., & Seeni, A. (2022). Investigation of antiproliferative mechanisms of Alstonia angustiloba-silver nanoparticles in skin squamous cell carcinoma (A431 cell line). Journal of Molecular Structure, 1250(2), 131814. https://doi.org/10.1016/j.molstruc.2021.131814

Ramachandran, S., Rajasekaran, A., & Manisenthilkumar, K. (2012). Investigation of hypoglycemic, hypolipidemic and antioxidant activities of aqueous extract of Terminalia paniculata bark in diabetic rats. Asian Pacific Journal of Tropical Biomedicine, 2(4), 262–268. https://doi.org/10.1016/S2221-1691(12)60020-3

Tajbakhsh, E., Kwenti, T. E., Kheyri, P., Nezaratizade, S., Lindsay, D. S., & Khamesipour, F. (2021). Antiplasmodial, antimalarial activities and toxicity of African medicinal plants: a systematic review of literature. Malaria Journal, 20(1), 349. https://doi.org/10.1186/s12936-021-03866-0

Tan, M. C. S., Carranza, M. S. S., Linis, V. C., Malabed, R. S., & Oyong, G. G. (2019). Antioxidant, Cytotoxicity, and Antiophidian Potential of Alstonia macrophylla Bark. ACS Omega, 4(5), 9488–9496. https://doi.org/10.1021/acsomega.9b00082

Tepongning, R. N., Lucantoni, L., Nasuti, C. C., Dori, G. U., Yerbanga, S. R., Lupidi, G., Marini, C., Rossi, G., Esposito, F., & Habluetzel, A. (2011). Potential of a Khaya ivorensis – Alstonia boonei extract combination as antimalarial prophylactic remedy. Journal of Ethnopharmacology, 137(1), 743–751. https://doi.org/10.1016/j.jep.2011.06.036

Zubairi, S. I., & Jaies, N. S. (2014). Hibiscus rosa sinensis leaves: Analysis of proximate, antioxidant activities and inorganic compound. Malaysian Journal of Analytical Sciences, 18(2), 260–270. Retrieved from http://pkukmweb.ukm.my/mjas/

Zuraida, Z., Sulistiyani, S., Sajuthi, D., & Suparto, I. H. (2017). Fenol, Flavonoid, dan Aktivitas Antioksidan Pada Ekstrak Kulit Batang Pulai (Alstonia scholaris R.Br). Jurnal Penelitian Hasil Hutan, 35(3), 211–219. https://doi.org/10.20886/jphh.2017.35.3.211-219

Author Biographies

Maria Nindatu, Biology Departement, FMIPA Pattimura University

Matha Kaihena, University of Pattimura, Ambon

Adriana Hieriej, University of Pattimura, Ambon

Amos Killay, University of Pattimura, Ambon

Hery Jotlely, Indonesia Christian Maluku University, Ambon

License

Copyright (c) 2023 Maria Nindatu, Matha Kaihena, Adriana Hieriej, Amos Killay, Hery Jotlely

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).