Phytochemical Screening and Antibacterial Activity Test of Sumbawa white honey against Bacillus megaterium

Authors

Nurmi Hasbi , Rosyunita , Adelia Riezka Rahim , Eusthacius Hagni Wardoyo , Ida Ayu Arnawati , Saskia Safarina Haza , Lale Nandhita Hulfifa , Abiel Dwi Cahya Firdaus Alamsyah , I Komang Satya Validika

DOI:

10.29303/jppipa.v9i10.4913

Published:

2023-10-25

Issue:

Vol. 9 No. 10 (2023): October

Keywords:

antibacterial, Bacillus megaterium, white honey sumbawa

Research Articles

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Hasbi, N., Rosyunita, R., Rahim, A. R. ., Wardoyo, E. H. ., Arnawati, I. A. ., Haza, S. S. ., … Validika, I. K. S. . (2023). Phytochemical Screening and Antibacterial Activity Test of Sumbawa white honey against Bacillus megaterium. Jurnal Penelitian Pendidikan IPA, 9(10), 8707–8712. https://doi.org/10.29303/jppipa.v9i10.4913
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Abstract

Bacillus megaterium is a bacteria that causes human infections, causing keratitis, skin infections, brain abscesses and tissue infections. Treatment often uses chemical antibiotics. However, long-term antibiotics can cause new health problems such as resistance. Therefore, antibacterial ingredients are needed that can not only inhibit the growth of bacteria but also do not have a negative impact on health such as honey. Honey is thick, it can cure various diseases such as gastrointestinal, stomach, skin diseases, acute respiratory infections and coughs. This research aims to determine the ability of Sumbawa white honey as an antibacterial against B. megaterium. The method used in this research is paper disc diffusion. There were five treatments used in this study, between 100%, 75% and 25% honey concentrations, DMSO negative control and chloramphenicol positive control with five replications each. The results of the antibacterial activity test showed that the 100% concentration had an inhibitory zone diameter of 2.59 mm, then the 75% concentration was 1.46 while the 25% concentration was 0 mm. When compared with positive control, white honey was lower antibacterial. If it is in the white honey category, the inhibition zone category is included in the lower category.

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Author Biographies

Nurmi Hasbi, Universitas Mataram

Rosyunita, Universitas Mataram

Adelia Riezka Rahim, Universitas Mataram

Eusthacius Hagni Wardoyo, Universitas Mataram

Ida Ayu Arnawati, Universitas Mataram

Saskia Safarina Haza, Universitas Mataram

Lale Nandhita Hulfifa, Universitas Mataram

Abiel Dwi Cahya Firdaus Alamsyah, Universitas Terbuka

I Komang Satya Validika, Universitas Mataram

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Copyright (c) 2023 Nurmi Hasbi, Rosyunita, Adelia Riezka Rahim, Eusthacius Hagni Wardoyo, Ida Ayu Arnawati, Saskia Safarina Haza, Lale Nandhita Hulfifa, Abiel Dwi Cahya Firdaus Alamsyah, I Komang Satya Validika

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