Antifungal Activity of Red Ginger (Zingiber officinale var. Rubrum) and Garlic (Allium sativum) against HIV Patients-Isolated Candida albicans

Authors

Erni Winarni , Ali Napiah Nasution , Sri Wahyuni Nasution

DOI:

10.29303/jppipa.v9i10.4981

Published:

2023-10-25

Issue:

Vol. 9 No. 10 (2023): October

Keywords:

Antifungal, Candida albicans, Garlic, HIV, Red ginger

Research Articles

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Winarni, E. ., Nasution, A. N. ., & Nasution, S. W. . (2023). Antifungal Activity of Red Ginger (Zingiber officinale var. Rubrum) and Garlic (Allium sativum) against HIV Patients-Isolated Candida albicans . Jurnal Penelitian Pendidikan IPA, 9(10), 9038–9044. https://doi.org/10.29303/jppipa.v9i10.4981

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Abstract

The most of Candida strain in oral candidiasis is nystatin resistance strain. Hence, it become important to investigate the natural products like red ginger (Zingiber officinale var. Rubrum) and garlic (Allium sativum) as antifungal against HIV patient-isolated Candida albicans. This experimental study used two antifungal assays (Disc diffusion and antibiofilm assay). Both red ginger and garlic extract were extracted by maceration method. Candida albicans isolate was isolated from a volunteer HIV Patient with oral candidiasis. The outcomes included the diameter of the inhibition zone and optical density (OD) that were expressed as mean and standard deviation and analysed by Kruskal-Walis. This study showed that the highest concentration of both red ginger (0.67 ± 0.55 mm) and garlic extract (0.67 ± 0.46 mm) had the widest diameter of inhibition zone. Meanwhile, the highest inhibition of biofilm formation was found in the lowest concentrations (25%) of red ginger (2.22 ± 1.24) and garlic extract (3.65 ± 0.24). Any concentration of red ginger or garlic extract did not significantly affect the antifungal activity (P-Value: 0.392). Overall, it can be concluded that both red ginger and garlic extract at any concentration showed a weak antifungal activity against HIV patient-isolated Candida albicans strain.

References

Adhikari, K. L. N., Bastola, A., Devkota, L., Bhandari, P., Dhungel, B., Bipin Adhikari, U. T. S., Banjara, M. R., Rijal, K. R., & Ghimire, P. (2020). Biofilm-producing candida species causing oropharyngeal candidiasis in hiv patients attending sukraraj tropical and infectious diseases hospital in kathmandu, nepal. HIV/AIDS - Research and Palliative Care, 12, 211–220. https://doi.org/10.2147/HIV.S255698

Aghazadeh, M., Bialvaei, A. Z., Aghazadeh, M., Kabiri, F., Saliani, N., Yousefi, M., Eslami, H., & Kafil, H. S. (2016). Survey of the antibiofilm and antimicrobial effects of Zingiber officinale (In vitro study). Jundishapur Journal of Microbiology, 9(2), 1–6. https://doi.org/10.5812/jjm.30167

Aidin, A., Sahiri, N., & Madauna, I. (2016). The Effect of Rhizome Types and The Composition of Planting Media on The Growth of Red Ginger (Zingiber Officinale Rosc.). E-J. Agrotekbis, 4(4), 394–402. Retrieved from http://jurnal.faperta.untad.ac.id/index.php/agrotekbis/article/view/37

Andayani, D., & Kurniawan, R. A. (2014). Uji Daya Hambat Ekstrak Etanol Bawang Putih Tunggal (Allium Sativum L.) terhadap Jamur (Candida Albicans) Dahlia. Jurnal Ilmu Kesehatan Dan Farmasi, 2(1), 15–19. Retrieved from https://ejournal.unwmataram.ac.id/jikf/article/view/124

Chiuman, L. (2019). Protective Effect of Curcuma zedoria Against Copper in Rats’ White Blood Cell. American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS), 59(1), 142–148. Retrieved from https://core.ac.uk/download/pdf/235050884.pdf

Chiuman, L., Sherlyn, Aritonang, N. S., Rudy, & Suhartomi. (2023). In Vitro Study of Antibacterial Activity of Snake Fruit Extract against Extended Spectrum Beta Lactamase (ESBL) Escherichia coli. Jurnal Aisyah : Jurnal Ilmu Kesehatan, 8(2), 715–720. https://doi.org/10.30604/jika.v8i2.1962

de Paula, S. B., Morey, A. T., Santos, J. P., dos Santos, P. M. C., Gameiro, D. G., Kerbauy, G., Sena, E. M., Ueda, L. T., Carneiro, M., Pinge-Filho, P., Yamauchi, L. M., & Yamada-Ogatta, S. F. (2015). Oral Candida colonization in HIV-infected patients in Londrina-PR, Brazil: Antifungal susceptibility and virulence factors. Journal of Infection in Developing Countries, 9(12), 1350–1359. https://doi.org/10.3855/jidc.6970

Fahim, A., Himratul-Aznita, W. H., Abdul-Rahman, P. S., & Alam, M. K. (2022). Efficacy of bakuchiol-garlic combination against virulent genes of Candida albicans. PeerJ, 10(e12251), 1–13. https://doi.org/10.7717/peerj.12251

Gulo, K. N., Suhartomi, Saragih, A. D., Raif, M. A., & Ikhtiari, R. (2021). Antioxidant Activity of Flavonoid Compounds in Ethanol and Ethyl Acetate Extract from Citrus Sinensis. AIMS 2021 - International Conference on Artificial Intelligence and Mechatronics Systems, 1–6. https://doi.org/10.1109/AIMS52415.2021.9466078

Harijani, N., Rahadi, U. S. E., & Nazar, D. S. (2013). The Isolation of Escherichia coli from the Meat Some Traditional Markets in the South Surabaya. Veterinaria Medika, 6(1), 39–44. Retrieved from https://journal.unair.ac.id/filerPDF/abstrak_614128_tpjua.pdf

Huang, F., Song, Y., Chen, W., Liu, Q., Wang, Q., Liu, W., Wang, X., & Wang, W. (2019). Effects of Candida albicans infection on defense effector secretion by human oral mucosal epithelial cells. Archives of Oral Biology, 103(February), 55–61. https://doi.org/10.1016/j.archoralbio.2019.05.013

Ibrahim, A. M., Sriherfyna, F. H., & Yunianta. (2015). Pengaruh Suhu dan Lama Waktu Ekstraksi Terhadap Sifat Kimia dan FIsik pada Pembuatan Minuman Sari JAhe Merah (Zingiber officinale var. Rubrum) dengan Kombinasi Penambahan Madu sebagai Pemanis. Jurnal Pangan Dan Agroindustri, 3(2), 530–541. Retrieved from https://jpa.ub.ac.id/index.php/jpa/article/view/171

Indraswari, N. M. W. S., Setyowati, D. I., & Hamzah, Z. (2022). Efektivitas Ekstrak Bawang Putih dan Bawang Hitam dalam Menghambat Pertumbuhan Candida albicans. Pustaka Kesehatan, 10(2), 114-119. Retrieved from https://garuda.kemdikbud.go.id/documents/detail/2895456

Irza, D. A., Lister, I. N. E., Sihotang, S., & Fachrial, E. (2021). Isolation, Characterization, Molecular Identification of Probiotic Bacteria from Meconium. IOP Conference Series: Earth and Environmental Science, 1–10. https://doi.org/10.1088/1755-1315/755/1/012041

Kalista, K., Lie, K., Wahyuningsih, R., & Rumende, C. (2017). Karakteristik Klinis dan Prevalensi Pasien Kandidiasis Invasif di Rumah Sakit Cipto Mangunkusumo. Jurnal Penyakit Dalam Indonesia, 4, 56. https://doi.org/10.7454/jpdi.v4i2.104

Kasetty, S., Mould, D. L., Hogan, D. A., & Nadell, C. D. (2021). Both Pseudomonas aeruginosa and Candida albicans Accumulate Greater Biomass in Dual-Species Biofilms under Flow. American Society for Microbiology, 6(3), 2021. https://doi.org/10.1128/msphere.00416-21

Kaul, D. R., Flanders, S. A., Beck, J. M., Saint, S., Kartikawati, E., Deswati, D. A., Pramudita, B., Limper, M., Eeftinck Schattenkerk, D., de Kruif, M. D., van Wissen, M., Brandjes, D. P. M., Duits, A. J., van Gorp, E. C. M., Hendra, R., Masdeatresa, L., Almurdani, M., Abdulah, R., Haryani, Y., & Rahmawati, M. (2020). Antifungal Activity of Red Dragon Peel (Hylocereus polyrhizus). Jurnal Sabdariffarma, 21(1), 124–128.

Khalaf, A. A., Al-Aedany, A. J. M., & Hussein, S. F. (2020). Activity evaluation of ginger (Zingiber officinale) alcoholic extract against Candida albicans. AIP Conference Proceedings, 2290. https://doi.org/10.1063/5.0029718

Murtiastutik, D., Maharani, C. S., Rahmadewi, & Listiawan, M. Y. (2019). Nystatin profile on Candida species in HIV/AIDS patients with oral candidiasis: A phenomenology study. Journal of Pure and Applied Microbiology, 13(4), 2013–2019. https://doi.org/10.22207/JPAM.13.4.12

Mutia, M. S., Annisa, E., & Suhartomi. (2021). Anti-Bacterial Activity of Ethanol Extract of Indian Borage (Coleus Amboinicus) Leaves against Bacillus Cereus. Healthy Tadulako Journal (Jurnal Kesehatan Tadulako), 7(1), 30–34. https://doi.org/10.22487/htj.v7i1.151

Mutiawati, V. K. (2016). Pemeriksaan Mikrobiologi pada Candida albicans. Jurnal Kedokteran Syiah Kuala, 16(1), 2016. https://doi.org/10.1016/s0035-9203(03)90055-1

Nasution, A. N., Sari Mutia, M., & Susilo, R. I. (2023). Antibacterial Activity of Mahkota Dewa (Phaleria macrocarpa) Leaves Extract against Propionibacterium acne. Jurnal Teknologi Laboratorium, 11(1), 4–13. https://doi.org/10.29238/teknolabjournal.v12i1.393

Noguchi, H., Iwase, T., Omagari, D., Asano, M., Nakamura, R., Ueki, K., Shinozuka, K., Kaneko, T., Tonogi, M., & Ohki, H. (2017). Rapid detection of Candida albicans in oral exfoliative cytology samples by loop-mediated isothermal amplification. Journal of Oral Science, 59(4), 541–547. https://doi.org/10.2334/josnusd.16-0717

Rahmatika, D., & Oktaria, S. (2021). Perbedaan Uji Daya Antibakteri Jahe Merah (Zingiber Officinale Var. Rubrum) Dan Bawang Putih (Allium Sativum) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus. Jurnal Kedokteran Ibnu Nafis, 10(1), 1–8. https://doi.org/10.30743/jkin.v10i1.94

Ratha, P. P. (2019). The Characteristics of Opportunistic Infections Among AIDS Patiaents in BRSU Tabanan in 2019. WMJ (Warmadewa Medical Journal), 4(2), 50–56. Retrieved from https://www.ejournal.warmadewa.ac.id/index.php/warmadewa_medical_journal/article/view/1201

Sabila, P., Ngadiani, & Budiarti, F. F. (2019). Uji Banding Ekstrak Bawang Hitam dan Ekstrak Bawang Putih (Allium sativum) Sebagai Antifungi Terhadap Pertumbuhan Candida albicans. Journal of Pharmacy and Science, 4(2), 101–104. https://doi.org/10.53342/pharmasci.v4i2.147

Sadeghi, G., Mousavi, S. F., Ebrahimi-Rad, M., Mirabzadeh-Ardekani, E., Eslamifar, A., Shams-Ghahfarokhi, M., Jahanshiri, Z., & Razzaghi-Abyaneh, M. (2020). In vivo and in vitro pathogenesis and virulence factors of candida albicans strains isolated from cutaneous candidiasis. Iranian Biomedical Journal, 24(5), 319–327. https://doi.org/10.29252/ibj.24.5.319

Samsudin, R. R., Kunsah, B., & Widyastuti, R. (2017). The effect of pacitan’s sweet orange’s (Citrus sinensis (L.) osbeck) peel powder on the lipid profile of male dyslipidemia rats (Rattus novergicus). Bali Medical Journal, 6(3), 51. https://doi.org/10.15562/bmj.v6i3.720

Saputri, L., Hastuti, E. D., & Budihastuti, R. (2018). Respon Pemberian Pupuk Urea dan Pupuk Kandang terhadap Pertumbuhan dan Kandungan Minyak Atsiri Tanaman Jahe Merah [Zingiber officinale (L,) Rosc var. rubrum]. Jurnal Biologi, 7(1), 1–7. Retrieved from https://ejournal3.undip.ac.id/index.php/biologi/article/view/19615

Suhartomi, Gulo, K. N., Saragih, A. D., Martinus, A. R., & Ikhtiari, R. (2020). Antioxidant Properties of Sweet Orange Peels in Several Fractions of Methanolic Extract. Proceedings of the International Conference on Health Informatics and Medical Application Technology - Volume 1: ICHIMAT, 371–378. https://doi.org/10.5220/0009515503710378

Suraini, & Putri, N. (2018). Efektifitas Anti Jamur Campuran Rebusan Jahe (Zingiber officinale) dan Kunyit (Curcuma domestica) terhadap Pertumbuhan Candida albicans. Prosiding Seminar Kesehatan Perintis, 1(2), 73–77. Retrieved from https://jurnal.upertis.ac.id/index.php/PSKP/article/download/126/119

Tsui, C., Kong, E. F., & Jabra-Rizk, M. A. (2016). Pathogenesis of Candida albicans Biofilm. Journals Investing in Science, 74(4), 1–13. https://doi.org/10.1093/femspd/ftw018

Zainal, M., Mohamad Zain, N., Mohd Amin, I., & Ahmad, V. N. (2021). The antimicrobial and antibiofilm properties of allicin against Candida albicans and Staphylococcus aureus – A therapeutic potential for denture stomatitis. Saudi Dental Journal, 33(2), 105–111. https://doi.org/10.1016/j.sdentj.2020.01.008

ZdanaviÄienÄ—, E., SakalauskienÄ—, J., Gleiznys, A., Gleiznys, D., & Žilinskas, J. (2017). Host responses to Candida albicans. A review. Stomatologija, 19(4), 109–123. Retrieved from https://www.sbdmj.com/174/174-02.pdf

Author Biographies

Erni Winarni, Universitas Prima Indonesia

Ali Napiah Nasution, Universitas Prima Indonesia

Sri Wahyuni Nasution, Universitas Prima Indonesia

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