IDENTIFIKASI SENYAWA METABOLIT SEKUNDER PADA HASIL FRAKSINASI EKSTRAK Phaseolus vulgaris L. DENGAN METODE GAS CHROMATOGRAPHY-MASS SPECTROSCOPY (GC-MS)

Authors

Khurriyatul Khair , Yayuk Andayani , Aliefman Hakim

DOI:

10.29303/jppipa.v3i1.51

Published:

2017-01-31

Issue:

Vol. 3 No. 1 (2017): January

Research Articles

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Khair, K., Andayani, Y., & Hakim, A. (2017). IDENTIFIKASI SENYAWA METABOLIT SEKUNDER PADA HASIL FRAKSINASI EKSTRAK Phaseolus vulgaris L. DENGAN METODE GAS CHROMATOGRAPHY-MASS SPECTROSCOPY (GC-MS). Jurnal Penelitian Pendidikan IPA, 3(1). https://doi.org/10.29303/jppipa.v3i1.51
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Abstract

This research was aimed to identify some classes of secondary metabolite compound on the fractionation results of green bean extract (P. vulgaris L.) using Gas Chromatography-Mass Spectroscopy (GC-MS) method. Green bean was extracted by methanol solvent. Fractionation of methanol extract used Vacuum Liquid Chromatography (VLC) with some variation of eluents such as n-hexane 100%; n-hexane: ethyl acetate (9 : 1 to 1 : 9); and ethyl acetate 100%, produced 11 major fractions.  Based on Thin Layer Chromatography (TLC) analysis using DCM: MeOH (9.5: 0.5) as mobile phases, these 11 major fractions were classified based on their polarity such as non-polar, semi-polar and polar fraction. The results of identification by GC-MS spectrometer showed the presence of secondary metabolites such as monoterpenes in non-polar fraction; in semi-polar fraction was found terpenoids and steroids; in polar fraction was found monoterpenes, phenolic and steroid as the lowest percent area, that is less than 2%.

 

Keyword: P.vulgaris L extract, fractionation, VLC, GC-MS

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