Effectiveness of Administering Red Belt Leaf Extract (Piper Crocatum) Against Aeromonas Hydrophila Bacteria in Vitro

Authors

Muhammad Jusril , Arief Prajitno , Mohamad Fadjar

DOI:

10.29303/jppipa.v10i3.6074

Published:

2024-03-30

Issue:

Vol. 10 No. 3 (2024): March

Keywords:

Aeromonas hydrophila, Antibacterial activity, Phytochemical test, Piper crocatum

Research Articles

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Jusril, M., Prajitno, A., & Fadjar, M. (2024). Effectiveness of Administering Red Belt Leaf Extract (Piper Crocatum) Against Aeromonas Hydrophila Bacteria in Vitro . Jurnal Penelitian Pendidikan IPA, 10(3), 1025–1031. https://doi.org/10.29303/jppipa.v10i3.6074
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Abstract

Diseases caused by pathogenic bacteria such as Aeromonas hydrophila are a major health concern. However, the use of red betel leaves (P. crocatum) can serve as a potential solution to this problem due to their antibacterial properties. This research aimed to study the antibacterial activity of red betel leaf extract (P. crocatum), the structure and character of the bacteria inhibited by the extract, and the bioactive components present in the leaves. The results of the disc test showed that the average zone of inhibition was 14.28 mm, while the MIC test results revealed that a dose of 100 mg/L could inhibit bacterial growth (bacteriostatic). Moreover, the crude extract of red betel leaf (P. crocatum) damaged the cell walls and cell membranes of A. hydrophila bacteria, thereby affecting their character and structure. The UV-VIS analysis of red betel leaf extract (P. crocatum) and FTIR analysis with 96% ethanol absorption indicated the presence of flavonoids, saponins, tannins, and alkaloids. These findings suggest that red betel leaves can be used as a natural remedy to fight bacterial infections caused by pathogenic bacteria such as Aeromonas hydrophila

References

Alchaddad, M., Siadi, K., & Supartono. (2015). Transesterifikasi Etil P-Metoksisinamat Hasil Isolasi Rimpang Kencur Dengan Vitamin C Terkatalis Lipase. Indonesian Journal of Chemical Science, 4(2), 84–88. Retrieved from https://journal.unnes.ac.id/sju/index.php/ijcs/article/view/6165/4668

Amanu, S., Kurniasih, & Indaryulianto, S. (2014). Identifikasi penyakit aeromonad pada budi daya ikan air tawar di Bali. Jurnal Veteriner, 15(4), 474–486. Retrieved from https://ojs.unud.ac.id/index.php/jvet/article/view/13226

Anarkhis, N., Prajitno, A., Maftuch, M., & Karimah, K. (2023). Activity Test of Antibacterial Compounds of Aloe Vera Extract Against Edwardsiella Tarda Bacterial Infection in Vitro (technique). Jurnal Penelitian Pendidikan IPA, 9(2), 563–567. https://doi.org/10.29303/jppipa.v9i2.2610

Athaillah, A., & Lestari, U. D. (2020). Antibacterial Activity Test of Ethanol Extract from Dried Simplicia Garlic (Allium sativum L.) Against Bacillus cereus Bacteria. Journal of Pharmaceutical And Sciences, 3(2), 93–99. https://doi.org/10.36490/journal-jps.com.v3i2.51

Egra, S., Mardhiana, M., Rofin, M., Adiwena, M., Jannah, N., Kuspradini, H., & Mitsunaga, T. (2019). Aktivitas Antimikroba Ekstrak Bakau (Rhizophora mucronata) dalam Menghambat Pertumbuhan Ralstonia Solanacearum Penyebab Penyakit Layu. Agrovigor: Jurnal Agroekoteknologi, 12(1), 26. https://doi.org/10.21107/agrovigor.v12i1.5143

Ernawati, K. (2015). Kandungan senyawa kimia dan aktivitas antibakteri ekstrak kulit buah alpukat terhadap bakteri Vibrio auginoliticus. Jurnal Kajian Veteriner, 16(2), 203–211. https://doi.org/10.35508/jkv.v3i2.1043

Fatmawati, F., Noor Arida Fauzana, N. A. F., Siti Aisiah, S. A., Ririen Kartika Rini, R. K. R., Olga, O., Tanod, W. A., & Putut Har Riyadi, P. H. R. (2021). Chemicals Profile of Kelakai Leaves Extracts (Stenochlaena palustris) with Antioxidant and Antibacterial Activity against Aeromonas hydrophila. Sains Malaysiana, 51(8), 2531–2546. https://doi.org/10.17576/jsm-2022-5108-14

Gerrine, G., Prajitno, A., Fadjar, M., & Kenitasari, R. E. (2023). Effects of Keji Beling (Strobilanthes crispus) Crude Leaves Extract Against Aeromonas hydrophila Bacterial Infection in Vitro. Jurnal Penelitian Pendidikan IPA, 9(5), 3849–3855. https://doi.org/10.29303/jppipa.v9i5.3502

Hariati, S., Wahjuningrum, D., Yuhana, M., Tarman, K., Effendi, I., & Saputra, F. (2018). Aktivitas Antibakteri Ekstrak Kapang Laut Nodulisporium sp. KT29 terhadap Vibrio harveyi. Jurnal Pengolahan Hasil Perikanan Indonesia, 21(2), 251. https://doi.org/10.17844/jphpi.v21i2.22855

Irwandi, D., & Sukmawati, A. E. (2020). Minimum Inhibitory Concentration (MIC) Of Liquid Smoke Produced from Piper Betle L Against Salmonella sp and Staphylococcus aureus. SANITAS: Jurnal Teknologi Dan Seni Kesehatan, 11(1), 89–98. https://doi.org/10.36525/sanitas.2020.8

Kumayas, A. R., Wewengkang, D. S., & Sudewi, S. (2015). Aktifitas Antibakteri dan Karateristik Gugus Fungsi dari Tunikata Polycarpa Aurata. PHARMACON Jurnal Ilmiah Farmasi, 4(1), 32–44. Retrieved from https://ejournal.unsrat.ac.id/v3/index.php/pharmacon/article/view/6481

Mabruroh, E. Q., Mursiti, S., & Kusumo, E. (2019). Isolasi dan identifikasi senyawa flavonoid dari daun murbei (Morus alba Linn. Indonesian Journal of Chemical Science, 8(1), 16–22. Retrieved from http://journal.unnes.ac.id/sju/index.php/ijcs

Marfuah, I., Dewi, E. N., & Rianingsih, L. (2018). Kajian Potensi Ekstrak Anggur Laut (Caulerpa racemosa) Sebagai Antibakteri Terhadap Bakteri Escherichia coli dan Staphylococcus aureus. Peng. & Biotek, 7(1), 7–14. Retrieved from https://rb.gy/lvfr1f

Marwulan, M., Nursyam, H., & Kilawati, Y. (2023). Antibacterial Activity Test of Tembelekan Leaf Extract (Lantana camara linn) against Edwardsiella tarda bacteria. Jurnal Penelitian Pendidikan IPA, 9(5), 3934–3942. https://doi.org/10.29303/jppipa.v9i5.3459

Navirius, F. J., Pamudji, G., & Herowati, R. (2023). Effect of Red Betel (Piper crocatum) Leaf Ethanol Extract On Increased Creatinine and Ureum Levels In White Rat Wistar Strain Induced Streptozotosin-Nikotinamid. Media Farmasi: Jurnal Ilmu Farmasi, 20(2), 57. https://doi.org/10.12928/mf.v20i2.25457

Pambudi, A. R., Wasiaturrahmah, Y., & Aspriyanto, D. (2021). Antibacterial Effectiveness Of Kecapi Sentul Extract (Sandoricum Koetjape Merr.) Against Streptococcus Mutans. ODONTO: Dental Journal, 8(2), 1. https://doi.org/10.30659/odj.8.2.1-10

Rahmah, N. N., Bahar, M., Fauziah, C., & Pramesyanti, A. (2023). Ekstrak daun sirih merah (Piper ornatum) berpotensi untuk mengurangi pertumbuhan Pseudomonas aeruginosa secara in vitro. Indonesian Journal of Biotechnology and Biodiversity, 7(1), 46–50. https://doi.org/10.47007/ijobb.v7i1.164

Seniati, Marbiah, & Irham, A. (2019). Pengukuran Kepadatan Bakteri Vibrio harveyi Secara Cepat Dengan Menggunakan Sektrofotometer. Agrokompleks, 19(2), 12–19. https://doi.org/10.51978/japp.v19i2.137

Sofidiana, L. L., Sulistyani, E., & Lestari, P. E. (2022). Daya hambat kombinasi ekstrak pegagan (Centella asiatica, L.) dan peppermint terhadap pertumbuhan Streptococcus mutans. Pustaka Kesehatan, 10(3), 195–201. https://doi.org/10.19184/pk.v10i3.25232

Sulistiyono, F. D., Sofihidayati, T., & Lohitasari, B. (2018). Uji Aktivitas Antibakteri Dan Fitokimia Kulit Bawang Merah (Allium cepa L.) Hasil Ekstraksi Metode Microwave Assisted Extraction (MAE). Mandala Of Health, 11(2), 71. https://doi.org/10.20884/1.mandala.2018.11.2.1316

Tiwow, V. A., Rampe, M. J., Rampe, H. L., & Apita, A. (2021). Pola Inframerah Arang Tempurung Kelapa Hasil Pemurnian Menggunakan Asam. Chemistry Progress, 14(2), 116. https://doi.org/10.35799/cp.14.2.2021.37191

Triyasmono, L., Ulfah, A., Rizki, M. I., Anwar, K., Wianto, T., & Santoso, H. B. (2020). FTIR and Chemometrics Application on Determination of Total Flavonoid Content of Pasak Bumi Root Extract (Eurycoma longifolia Jack.). Jurnal Pharmascience, 7(2), 129. https://doi.org/10.20527/jps.v7i2.7923

Author Biographies

Muhammad Jusril, University of Brawijaya

Arief Prajitno, Brawijaya University

Mohamad Fadjar, University of Brawijaya

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Copyright (c) 2024 Muhammad Jusril, Arief Prajitno, Mohamad Fadjar

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