Phylogenetic and Genetic Diversity of the Spiny Eel Mastacembelus unicolor (Mastacembelidae) from Kediri, East Java, Indonesia

Authors

Ifa Sufaichusan , Maheno Sri Widodo , Ating Yuniarti , Bela Fatma Hani Ayu Lestari , Wahyu Endra Kusuma

DOI:

10.29303/jppipa.v10i10.6138

Published:

2024-10-25

Issue:

Vol. 10 No. 10 (2024): October

Keywords:

COI, genetic diversity, Identification, Mastacembelus, Phylogenetics

Research Articles

Downloads

How to Cite

Sufaichusan, I., Widodo, M. S., Yuniarti, A., Lestari, B. F. H. A., & Kusuma, W. E. (2024). Phylogenetic and Genetic Diversity of the Spiny Eel Mastacembelus unicolor (Mastacembelidae) from Kediri, East Java, Indonesia. Jurnal Penelitian Pendidikan IPA, 10(10), 7968–7975. https://doi.org/10.29303/jppipa.v10i10.6138
Crossref
Scopus
Google Scholar
Europe PMC

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

The spiny eel from the family Mastacembelidae are potential fish for aquaculture because it’s high economic value as an ornamental and as a consumption fish. Unfortunately, this fish have not been cultivated yet and up to present local people depend on wild-caught. This situation leads to severe population decline of this species in nature. Efforts to cultivate or protect this species in nature are therefore crucial. Species identification is the first and essential step performing domestication or conservation of a species. In this study, we conducted morphological identification as well as phylogenetic and genetic diversity analyses using cytochrom c oxidase subunit I gene. The results revealed spiny eel from Kediri can be identified as Mastacembelus unicolor based on both morphological and molecular analyses. The phylogenetic analyses suggested that individuals from Kediri have the closest relationship to M. unicolor individuals from West Java. Individuals sequenced from Kediri exhibit identical haplotype which indicating no genetic polymorphism between individuals. The individuals of Mastacembelidae in the Kediri were morphologically and genetically identified as Mastacembelus unicolor. The genetic analysis result shows Kediri have 53.13% AT, 48.67% GC, and one haplotype with low hd and low π value.

References

Akbar, N., P Zamani, N., & H Madduppa, H. (2014). Keragaman genetik ikan tuna sirip kuning (Thunnus albacares) dari dua populasi di Laut Maluku, Indonesia. Depik, 3(1). https://doi.org/10.13170/depik.3.1.1304

Aly, W., Ismail, M., & Ali, F. S. (2023). Exploring the presence and characteristics of spiny eels (Mastacembelidae, Teleostei) in the Nile Delta: An analysis of morphology, molecular phylogeny, and taxonomy. Egyptian Journal of Aquatic Research, 49(2), 151–160. https://doi.org/10.1016/j.ejar.2023.03.004

Anjarsari, Y., Syaifudin, M., Jubaedah, D., Taqwa, F. H., & Yonarta, D. (2021). Phylogenetic of Featherback Chitala sp from South Sumatra Based on Cytochrome C Oxidase Subunit I (COI) Gene. IOP Conference Series: Earth and Environmental Science, 810(1), 012009. https://doi.org/10.1088/1755-1315/810/1/012009

Ath-thar, M. H. F., Putriana, I., Soelistyowati, D. T., & Gustiano, R. (2017). Performa Genotip Ikan Tambakan Helostoma temminckii (CUVIER, 1829) Populasi Sumatera, Jawa Dan Kalimantan Dengan Metode Random Amplified Polymorphic DNA (RAPD). Jurnal Sains Natural, 4(1), 68. https://doi.org/10.31938/jsn.v4i1.77

Chiu, Y.-W., Bor, H., Tan, M.-S., Lin, H.-D., & Jean, C.-T. (2013). Phylogeography and Genetic Differentiation among Populations of the Moon Turban Snail Lunella granulata Gmelin, 1791 (Gastropoda: Turbinidae). International Journal of Molecular Sciences, 14(5), 9062–9079. https://doi.org/10.3390/ijms14059062

Dahruddin, H., Hutama, A., Busson, F., Sauri, S., Hanner, R., Keith, P., Hadiaty, R., & Hubert, N. (2017). Revisiting the ichthyodiversity of Java and Bali through DNA barcodes: taxonomic coverage, identification accuracy, cryptic diversity and identification of exotic species. Molecular Ecology Resources, 17(2), 288–299. https://doi.org/10.1111/1755-0998.12528

Duong, T.-Y., Tran, L. V. D., Nguyen, N.-T. T., Jamaluddin, J. A. F., & Azizah, M. N. S. (2020). Unravelling taxonomic ambiguity of the Mastacembelidae in the Mekong Delta (Vietnam) through DNA barcoding and morphological approaches. Tropical Zoology, 33(2), 63–76. https://doi.org/10.4081/tz.2020.72

Fietri, W. A., Rajak, A., & Sumarmin, R. (2021). Analisis Filogenetik Familia Ikan Kerapu Serranidae Berdasarkan Penandaan Chytocrome Oxydase I (COI) dari Pasar Ikan Lokal di Indonesia. Jurnal Biologi Papua, 13(2), 129–136. https://doi.org/10.31957/jbp.1471

Francis, C. M., Borisenko, A. V., Ivanova, N. V., Eger, J. L., Lim, B. K., Guillén-Servent, A., Kruskop, S. V., Mackie, I., & Hebert, P. D. N. (2010). The Role of DNA Barcodes in Understanding and Conservation of Mammal Diversity in Southeast Asia. PLoS ONE, 5(9), e12575. https://doi.org/10.1371/journal.pone.0012575

Frankham, R. (1997). Do island populations have less genetic variation than mainland populations? Heredity, 78(3), 311–327. https://doi.org/10.1038/hdy.1997.46

Gholamhosseini, A., Razbanian, M., Esmaeili, H. R., & Eagderi, S. (2022). Molecular systematics and morphological variation in the Mesopotamian spiny eel Mastacembelus mastacembelus (Teleostei: Mastacembelidae). The European Zoological Journal, 89(1), 546–555. https://doi.org/10.1080/24750263.2022.2057604

Grant, W., & Bowen, B. (1998). Shallow population histories in deep evolutionary lineages of marine fishes: insights from sardines and anchovies and lessons for conservation. Journal of Heredity, 89(5), 415–426. https://doi.org/10.1093/jhered/89.5.415

Hebert, P. D. N., Cywinska, A., Ball, S. L., & DeWaard, J. R. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences, 270(1512), 313–321. https://doi.org/10.1098/rspb.2002.2218

Hendiari, I. G. A. D., Sartimbul, A., Arthana, I. W., & Kartika, G. R. A. (2020). Keragaman genetik ikan lemuru (Sardinella lemuru) di wilayah perairan Indonesia. Acta Aquatica: Aquatic Sciences Journal, 7(1), 28. https://doi.org/10.29103/aa.v7i1.2405

Ketchum, R. N., Dieng, M. M., Vaughan, G. O., Burt, J. A., & Idaghdour, Y. (2016). Levels of genetic diversity and taxonomic status of Epinephelus species in United Arab Emirates fish markets. Marine Pollution Bulletin, 105(2), 540–545. https://doi.org/10.1016/j.marpolbul.2015.11.042

Kottelat, M., Whitten, J. A., Kartikasari, N. S., & Wirjoatmodji, S. (1993). Freshwater Fishes of Western Indonesia. Jakarta: Periplus Editions (HK) Ltd.

Kusuma, W. E., Sufaichusan, I., Lestari, B. F. H. A., & Widyawati, Y. (2023). Identifikasi Molekuler dan Posisi Filogenetik Ikan Sili (Mastacembelidae: Macrognathus) dari Sungai Brantas, Jawa Timur, berdasarkan DNA mitokondria Gen COI. Jurnal Sumberdaya Akuatik Indopasifik, 7(2), 161–174. https://doi.org/10.46252/jsai-fpik-unipa.2023.Vol.7.No.2.308

Lamadi, A. (2023). Integrative taxonomy and reproductive biology of Hulu’u fish (Eleotridae: Giuris margaritaceus) from Lake Limboto. Universitas Brawijaya.

Meilana, L., Wardiatno, Y., Butet, N. A., & Krisanti, M. (2016). Morphological Character And Molecular Identification With Coi Gene Marker Of Horseshoe Crabs (Tachypleus gigas) At Coastal Waters Of Northern Java Island. Jurnal Ilmu Dan Teknologi Kelautan Tropis, 8(1), 145–158. https://doi.org/10.29244/jitkt.v8i1.12651

Murdy, E. O., Kottelat, M., Whitten, A. J., Kartikasari, N., & Wirjoatmodjo, S. (1994). Freshwater Fishes of Western Indonesia and Sulawesi. Copeia, 3, 830. https://doi.org/10.2307/1447208

Nuryanto, A., Bhagawati, D., & Abulias, M. N. (2017). Fish diversity at Cileumeuh River in District of Majenang, Cilacap Regency, Central Java. Jurnal Iktiologi Indonesia, 12(2), 147–153. Retrieved from https://jurnal-iktiologi.org/index.php/jii/article/view/120

Parenti, L. R., & Nelson, J. S. (1995). Fishes of the World. In Copeia. John Wiley and Sons. https://doi.org/10.2307/1446836

Pramono, T. B., Arfiati, D., Widodo, M. S., & Yanuhar, U. (2019). Genetic characteristics of senggaringan fish (Mystus singaringan) from Klawing river, Brantas River and Thailand as the basis of conservation and domestication. Aquaculture, Aquarium, Conservation & Legislation, 12(4), 996–1004. Retrieved from http://www.bioflux.com.ro/docs/2019.996-1004.pdf

Prehadi, P., Sembiring, A., Kurniasih, E. M., Rahmad, R., Arafat, D., Subhan, B., & Madduppa, H. H. (2014). DNA barcoding and phylogenetic reconstruction of shark species landed in Muncar fisheries landing site in comparison with Southern Java fishing port. Biodiversitas Journal of Biological Diversity, 16(1), 55–61. https://doi.org/10.13057/biodiv/d160107

Rahma, Y. A. (2021). Kajian Morfologi Ikan Sili Pada Nasi Boranan Makanan Khas Daerah Lamongan Provinsi Jawa Timur. Jurnal Matematika Dan Sains, 1(1), 23–30. https://doi.org/10.55273/jms.v1i1.78

Saleky, D., & Dailami, M. (2021). Konservasi Genetik Ikan Kakap Putih (Lates calcarifer, Bloch, 1790) Melalui Pendekatan DNA Barcoding dan Analisis Filogenetik di Sungai Kumbe Merauke Papua. Jurnal Kelautan Tropis, 24(2), 141–150. https://doi.org/10.14710/jkt.v24i2.10760

Sentosa, A. arifi., & Adikusuma, A. (2011). Resource conservation resource conservation of berod fish (Mastacembelus sp.) in The Central Part Of The Cimanuk River, Sumedang. Proceedings of the National Forum for Encouraging Fish Resources, 3(1), 1–15. Retrieved from https://shorturl.at/ujflJ

Simbolon, A. R., & Aji, L. P. (2021). Molecular Identification and Philogenetic Structure of Mollusca (Gastropods and Bivalvians) in Biak Waters, Papua. BAWAL Widya Riset Perikanan Tangkap, 13(1), 11–21. https://doi.org/10.15578/bawal.13.1.2021.%25p

Slocombe, L., Al-Khalili, J. S., & Sacchi, M. (2021). Quantum and classical effects in DNA point mutations: Watson–Crick tautomerism in AT and GC base pairs. Physical Chemistry Chemical Physics, 23(7), 4141–4150. https://doi.org/10.1039/D0CP05781A

Symonová, R., & Suh, A. (2019). Nucleotide composition of transposable elements likely contributes to AT/GC compositional homogeneity of teleost fish genomes. Mobile DNA, 10(1), 49. https://doi.org/10.1186/s13100-019-0195-y

Tapia, H. J., Bárcenas-Argüello, M. L., Terrazas, T., & Arias, S. (2017). Phylogeny and Circumscription of Cephalocereus (Cactaceae) Based on Molecular and Morphological Evidence. Systematic Botany, 42(4), 1–15. https://doi.org/10.1600/036364417X696546

Thirumaraiselvi, R., & Thangaraj, M. (2015). Genetic Diversity Analysis of Indian Salmon, Eleutheronema tetradactylum from South Asian Countries Based on Mitochondrial COI Gene Sequences. Notulae Scientia Biologicae, 7(4), 417–422. https://doi.org/10.15835/nsb.7.4.9668

Thomsen, P. F., & Willerslev, E. (2015). Environmental DNA – An emerging tool in conservation for monitoring past and present biodiversity. Biological Conservation, 183(11), 4–18. https://doi.org/10.1016/j.biocon.2014.11.019

Triandiza, T., Kusnadi, A., Sari, N., Pesilette, R. N., Ainarwowan, A., Suparmo, & Sapulete, S. (2021). Genetic diversity of small giant clam Tridacna maxima in Kur Island, Biak Island and Manado and their implications to conservation. Jurnal Penelitian Perikanan Indonesia, 26(3), 167–179. Retrieved from http://ejournal-balitbang.kkp.go.id/index.php/jppi/article/view/9400

Ukenye, E. A., Taiwo, I. A., & Anyanwu, P. E. (2019). Morphological and genetic variation in Tilapia guineensis in West African coastal waters: A mini review. Biotechnology Reports, 24. https://doi.org/10.1016/j.btre.2019.e00362

Yunus, A., & Mulya, M. B. (2018). Morphological Study and Abundance of Sili Fish (Family: Mastacembelidae) in the Seruai River, Namu Suro Village, Deli Serdang Regency. Irwanmay, 14, 1–13. https://doi.org/10.15900/j.cnki.zylf1995.2018.02.001

Author Biographies

Ifa Sufaichusan, Universitas Brawijaya

Maheno Sri Widodo, Universitas Brawijaya

Ating Yuniarti, Universitas Brawijaya

Bela Fatma Hani Ayu Lestari, Universitas Brawijaya

Wahyu Endra Kusuma, Universitas Brawijaya

License

Copyright (c) 2024 Ifa Sufaichusan, Maheno Sri Widodo, Ating Yuniarti, Bela Fatma Hani Ayu Lestari, Wahyu Endra Kusuma

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).