Dermapen Action on the Skin of White Rats Wistar Strain (Rattus norvegicus) to Evaluate the Efficacy of Avocado Fruit Ethanol Extract Cream (Persea americana Mill.) in Healing Wounds

Authors

Tan Suyono , Rica Mucmaini , Chrismis Novalinda Ginting , Irza Haicha Pratama

DOI:

10.29303/jppipa.v9i12.6181

Published:

2023-12-20

Issue:

Vol. 9 No. 12 (2023): December

Keywords:

Avocado, Dermapen, Ethanol extract, Healing wounds, Skin

Research Articles

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How to Cite

Suyono, T. ., Mucmaini, R. ., Ginting, C. N. ., & Pratama, I. H. . (2023). Dermapen Action on the Skin of White Rats Wistar Strain (Rattus norvegicus) to Evaluate the Efficacy of Avocado Fruit Ethanol Extract Cream (Persea americana Mill.) in Healing Wounds. Jurnal Penelitian Pendidikan IPA, 9(12), 11697–11704. https://doi.org/10.29303/jppipa.v9i12.6181

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Abstract

Wounds disrupt anatomical and skin functions. Thus, proper treatment and healing are needed. Saponins, tannins, and flavonoids in natural medicinal extracts accelerate wound healing. Plants and natural substances are usually employed in topical therapy. This study tested the efficacy of ethanol extract cream from the avocado pulp (Persea americana Mill.) in treating dermapen-induced lesions on white rats (Rattus norvegicus) Wistar strain. This is a lab study or an experiment. Mouse samples were divided into four treatment groups of five mice each. This study also found that avocado flesh extract (Persea americana Mill.) significantly improved dermapen scar healing compared to base cream. The treatment groups given avocado pulp extract (Persea americana Mill.) exhibited higher healing percentages than the control group: 60.5, 95.7, 97.3, and 100%. Researchers found that 15% avocado flesh extract cream (Persea americana Mill.) healed dermapen scars best. This study found that avocado flesh extract cream (Persea americana Mill.) accelerates skin regeneration and increases fibroblasts more than base creams. The highest and densest number of fibroblast cells was found in treatment group 3 healing wounds. Fibroblasts indicate active dermapen wound healing.

References

AL-kahfaji, M. H. A. M. (2022). Human Skin Infection A Review Study. Biomedicine and Chemical Sciences, 1(4), 254–258. https://doi.org/10.48112/bcs.v1i4.259

Alster, T. S., & Graham, P. M. (2018). Microneedling: A Review and Practical Guide. Dermatologic Surgery, 44(3), 397–404. https://doi.org/10.1097/DSS.0000000000001248

Amer, M., Farag, F., Amer, A., ElKot, R., & Mahmoud, R. (2018). Dermapen in the Treatment of Wrinkles in Cigarette Smokers and Skin Aging Effectively. Journal of Cosmetic Dermatology, 17(6), 1200–1204. https://doi.org/10.1111/jocd.12748

Atiyeh, B. S., Ghanem, O. A., & Chahine, F. (2021). Microneedling: Percutaneous Collagen Induction (PCI) Therapy for Management of Scars and Photoaged Skin—Scientific Evidence and Review of the Literature. Aesthetic Plastic Surgery, 45(1), 296–308. https://doi.org/10.1007/s00266-020-01927-4

Bu, L., Dai, O., Zhou, F., Liu, F., Chen, J. F., Peng, C., & Xiong, L. (2020). Traditional Chinese Medicine Formulas, Extracts, and Compounds Promote Angiogenesis. Biomedicine and Pharmacotherapy, 132(September), 110855. https://doi.org/10.1016/j.biopha.2020.110855

Depkes. (1995). Materia Medika Indonesia (V & VI). Jakarta: Kementrian Kesehatan RI.

Duke, J. A., Bogenschutz-Godwin, M. J., DuCellier, J., Duke, P.-A. K., & Kumar, R. (2022). Handbook of Medicinal Herbs Second Edition (Kindle Edi, Vol. 5, Issue 1). Florida: CRC Press. https://doi.org/10.1097/00004850-199001000-00014

Fam, V. W., Charoenwoodhipong, P., Sivamani, R. K., Holt, R. R., Keen, C. L., & Hackman, R. M. (2022). Plant-Based Foods for Skin Health: A Narrative Review. Journal of the Academy of Nutrition and Dietetics, 122(3), 614–629. https://doi.org/10.1016/j.jand.2021.10.024

Fares, M. M., Radaydeh, S. K., & AlAmeen, H. M. (2023). Green Tannins/Avocado Oil Composites; Suncare and Skincare Materials. Arabian Journal of Chemistry, 16(6), 104764. https://doi.org/10.1016/j.arabjc.2023.104764

Ferreira, S. M., Falé, Z., & Santos, L. (2022). Sustainability in Skin Care: Incorporation of Avocado Peel Extracts in Topical Formulations. Molecules, 27(6), 1–16. https://doi.org/10.3390/molecules27061782

Ghomi, E. R., Khalili, S., Khorasani, S. N., Neisiany, R. E., & Ramakrishna, S. (2019). Wound Dressings: Current Advances and Future Directions. Journal of Applied Polymer Science, 136(27), 1–12. https://doi.org/10.1002/app.47738

Ghozali, I. (2018). Aplikasi Analisis Multivariate dengan Program IBM SPSS 25. Semarang: Badan Penerbit Universitas Diponegoro.

Guan, L. L., Lim, H. W., & Mohammad, T. F. (2021). Sunscreens and Photoaging: A Review of Current Literature. American Journal of Clinical Dermatology, 22(6), 819–828. https://doi.org/10.1007/s40257-021-00632-5

Hannon, B. A., Edwards, C. G., Thompson, S. V., Reeser, G. E., Burd, N. A., Holscher, H. D., Teran-Garcia, M., & Khan, N. A. (2020). Single Nucleotide Polymorphisms Related to Lipoprotein Metabolism Are Associated with Blood Lipid Changes following Regular Avocado Intake in a Randomized Control Trial among Adults with Overweight and Obesity. Journal of Nutrition, 150(6), 1379–1387. https://doi.org/10.1093/jn/nxaa054

Hoseinkhani, Z., Norooznezhad, F., Rastegari-Pouyani, M., & Mansouri, K. (2020). Medicinal Plants Extracts with Antiangiogenic Activity: Where Is the Link? Advanced Pharmaceutical Bulletin, 10(3), 370–378. https://doi.org/10.34172/apb.2020.045

Iriarte, C., Awosika, O., Rengifo-Pardo, M., & Ehrlich, A. (2017). Review of Applications of Microneedling in Dermatology. Clinical, Cosmetic and Investigational Dermatology, 10, 289–298. https://doi.org/10.2147/CCID.S142450

Juhasz, M. L. W., & Cohen, J. L. (2020). Microneedling for the Treatment of Scars: An Update for Clinicians. Clinical, Cosmetic and Investigational Dermatology, 13, 997–1003. https://doi.org/10.2147/CCID.S267192

Kaban, V. E., Nasri, N., Syahputra, H. D., Fitri, R., Rani, Z., & Lubis, M. F. (2022). Formulasi Sediaan Gel dari Ekstrak Metanol Biji Alpukat (Persea americana Mill.) sebagai Penyembuh Luka Sayat pada Tikus Jantan (Rattus norvegicus). Herbal Medicine Journal, 5(2), 48–54. https://doi.org/10.58996/hmj.v5i2.50

Kupnik, K., PrimožiÄ, M., Kokol, V., Knez, Ž., & Leitgeb, M. (2023). Enzymatic, Antioxidant, and Antimicrobial Activities of Bioactive Compounds from Avocado (Persea americana L.) Seeds. Plants, 12(5). https://doi.org/10.3390/plants12051201

Lister, I. N. E., Amiruddin, H. L., Fachrial, E., & Girsang, E. (2021). Anti-Aging Effectiveness of Avocado Peel Extract Ointment (Persea americana Mill.) against Hydration, Collagen, and Elasticity Levels in Wistar Rat. Journal of Pharmaceutical Research International, February, 173–184. https://doi.org/10.9734/jpri/2021/v33i32b31760

Marini, M., Nurnabhilah, E., & Pratiwi, D. (2020). Formulasi dan Evaluasi Sediaan Krim Ekstrak Etanol Buah Alpukat (Persea americana Mill). HERBAPHARMA: Journal of Herbs and Farmacological, 2(1), 31–35.

Megawati, S., Ummah, U. C., & Setiawan, A. A. (2020). Formulasi dan Uji Efektivitas Penyembuhan Luka Sayat Salep Ekstrak Metanol Bunga Ginje (Thevetia Peruviana) terhadap Kelinci Jantan New Zealand White. Jurnal Farmasi Udayana, Desember, 180–186. https://doi.org/10.24843/jfu.2020.v09.i03.p06

Nilforoushzadeh, M. A., Amirkhani, M. A., Zarrintaj, P., Moghaddam, A. S., Mehrabi, T., Alavi, S., & Sisakht, M. M. (2018). Skin Care and Rejuvenation by Cosmeceutical Facial Mask. Journal of Cosmetic Dermatology, 17(5), 693–702. https://doi.org/10.1111/jocd.12730

Notoatmodjo, S. (2018). Metodologi Penelitian Kesehatan (3rd ed.). Jakarta: Rineka Cipta.

Pamungkas, W. T., & Wahyuningsih, S. S. (2022). Efek Pemberian Ekstrak Etanol Daun Alpukat (Persea americana Mill) terhadap Penyembuhan Luka Bakar pada Kelinci (Oryctolagus Cunicullus). Indonesian Journal on Medical Science, 9(1), 33–39. https://doi.org/10.55181/ijms.v9i1.348

Prastika, D. D., Setiawan, B., Saputro, A. L., Yudaniayanti, I. S., Wibawati, P. A., & Fikri, F. (2020). Effect of Shrimp Chitosan Topically on Collagen Density as Excision Wound Healing Parameter in Albino Rats. Jurnal Medik Veteriner, 3(1), 101–107. https://doi.org/10.20473/jmv.vol3.iss1.2020.101-107

Russell, W. M. (1995). The Development of the Three RS Concept. Alternatives to Laboratory Animals: ATLA, 23(3), 298–304. https://doi.org/10.1177/026119299502300306

Salman, H. A., & Mohammed, Z. K. (2020). Treatment of Acne Scar Using Automated Microneedling Device of 2.5 Mm versus 1.5 Mm A Split Face Study. Iraqi Postgraduate Medical Journal, 19(2), 110–118. Retrieved from https://iraqjournals.com/article_167093_0.html

Schmitt, L., Marquardt, Y., Amann, P., Heise, R., Huth, L., Wagner-Schiffler, S., Huth, S., & Baron, J. M. (2018). Comprehensive Molecular Characterization of Microneedling Therapy in A Human Three-Dimensional Skin Model. PLoS ONE, 13(9), 1–11. https://doi.org/10.1371/journal.pone.0204318

Sonenblum, S. E., Patel, R., Phrasavath, S., Xu, S., & Bates-Jensen, B. M. (2023). Using Technology to Detect Erythema Across Skin Tones. Advances in Skin and Wound Care, 36(10), 524–533. https://doi.org/10.1097/ASW.0000000000000043

Suwarno, B., & Nugroho, A. (2023). Kumpulan Variabel-Variabel Penelitian Manajemen Pemasaran (Definisi & Artikel Publikasi) (1st ed.). Bogor: Halaman Moeka Publishing.

Trujillo-Mayol, I., Casas-Forero, N., Pastene-Navarrete, E., Silva, F. L., & Alarcón-Enos, J. (2021). Fractionation and Hydrolyzation of Avocado Peel Extract: Improvement of Antibacterial Activity. Antibiotics, 10(1), 1–22. https://doi.org/10.3390/antibiotics10010023

Vincent, C., Mesa, T., & Munneâ€Bosch, S. (2020). Identification of A New Variety of Avocados (Persea americana Mill. Cv. Bacon) with High Vitamin E and Impact of Cold Storage on Tocochromanols Composition. Antioxidants, 9(5), 1–13. https://doi.org/10.3390/antiox9050403

Author Biographies

Tan Suyono, Faculty of Medicine, Dentistry and Health Sciences, Prima Indonesia University, Medan, Indonesia

Rica Mucmaini, Faculty of Medicine, Dentistry and Health Sciences, Prima Indonesia University, Medan, Indonesia

Chrismis Novalinda Ginting, Faculty of Medicine, Dentistry and Health Sciences, Prima Indonesia University, Medan, Indonesia

Irza Haicha Pratama, Faculty of Medicine, Dentistry and Health Sciences, Prima Indonesia University, Medan, Indonesia

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Copyright (c) 2023 Tan Suyono, Rica Mucmaini, Chrismis Novalinda Ginting, Irza Haicha Pratama

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