Synthesis, molecular docking, and in vitro tests of the Mannich base derivatives of Benzimidazolvanilin as an anti-inflammatory


Inas Priasti Siwi , Hayun , Arry Yanuar






Vol. 10 No. 4 (2024): April


Benzimidazolvanilin, Mannich base, Anti-inflammatory, Molecular docking

Research Articles


How to Cite

Siwi, I. P., Hayun, & Yanuar, A. (2024). Synthesis, molecular docking, and in vitro tests of the Mannich base derivatives of Benzimidazolvanilin as an anti-inflammatory . Jurnal Penelitian Pendidikan IPA, 10(4), 1760–1769.


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Benzimidazole and vanillin are compounds which can act as anti-inflammatory. However, the potential of these two compounds is still low. The structure of benzimidazole and vanillin was combined with Mannich base substitution in order to increase potency and selectivity as an anti-inflammatory. In this study, benzimidazolvanillin (1) and its Mannich base derivatives were synthesized. The synthesized compounds (2a-d) show anti-inflammatory activity based on in vitro tests by using protein denaturation inhibition test. Furthermore, the IC50 obtained ranged from 210 -243 μM. This potency is lower than diclofenac (IC50: 2,99μM). In molecular docking, the results of the inhibition constant ratio between COX-1 / COX-1 as well as the interaction of the ligand and target protein show that benzimimidazolvanillin and its Mannich-base derivatives could be anti-inflammatory drug candidates that should be investigated further.


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Author Biographies

Inas Priasti Siwi, University of Indonesia

Hayun, University of Indonesia, Jakarta

Arry Yanuar, University of Indonesia, Jakarta


Copyright (c) 2024 Inas Priasti Siwi, Hayun, Arry Yanuar

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