Detection of Resistance Gene from Aeromonas hydrophila Isolated from Catfish Farming in Jambi

Authors

titis wulandari , Tri Wiyoko , Tri Wera Agrita , Nabila Swarna Puspa Hermana , Agustin Indrawati

DOI:

10.29303/jppipa.v10i2.6868

Published:

2024-02-25

Issue:

Vol. 10 No. 2 (2024): February

Keywords:

Aeromonas hydrophila, Antibiotic, Catfish; Gene

Research Articles

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wulandari, titis, Wiyoko, T., Agrita, T. W. ., Hermana, N. S. P. ., & Indrawati, A. . (2024). Detection of Resistance Gene from Aeromonas hydrophila Isolated from Catfish Farming in Jambi. Jurnal Penelitian Pendidikan IPA, 10(2), 583–588. https://doi.org/10.29303/jppipa.v10i2.6868

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Abstract

Aeromonas hydrophila is one of the causative agents for Motile Aeromonas Septicemia (MAS) in catfish. MAS treatment is generally carried out by administering antibiotics. This study aimed to determine the antibiotic resistance profile of A. hydrophila and detect the presence of resistance genes. The samples are archived A. hydrophila isolates isolated from catfish ponds in the district of Muara Jambi, Bungo, Jambi Province, and Merangin total of 18 isolates. Testing the antibiotic resistance profile was carried out using the disk diffusion method against tetracycline, oxytetracycline, ampicillin, amoxicillin, ciprofloxacin, enrofloxacin, and streptomycin. The detected resistance genes were ampC, qnrA, qnrB, and qnrS. The results showed A. hydrophila isolates were resistant to tetracycline (100%), oxytetracycline (94.42%), ampicillin (77.70%), and amoxicillin (77.72%) and still sensitive to ciprofloxacin (83.32%), enrofloxacin (100 %), and streptomycin (100%). The ampC gene was found in 8 out of 14 isolates resistant to beta-lactam antibiotics (57.14%). The qnrA and qnrS genes were found in 3 out of 4 isolates resistant to quinolone antibiotics (75%), while qnrB was found in 1 isolate (25%). A. hydrophila isolated from catfish ponds in Jambi develops resistance to antibiotics and resistance genes found in isolates resistant to antibiotics.

References

Adesiji, Y. O., Deekshit, V. K., & Karunasagar, I. (2014). Antimicrobialâ€resistant genes associated with Salmonella spp. isolated from human, poultry, and seafood sources. Food Science & Nutrition, 2(4). https://doi.org/10.1002/fsn3.119

Alfiyanti, A., Sitasiwi, A. J., & Mardiati, S. M. (2019). Pengaruh Pemberian Ekstrak Etanol Daun Mimba (Azadirachta indica A.Juss) terhadap Berat Uterus dan Tebal Endometrium Mencit (Mus musculus L.). Buletin Anatomi Dan Fisiologi, 4(1). https://doi.org/10.14710/baf.4.1.2019.82-89

Anggraini, R., Aliza, D., & Mellisa, S. (2016). Identifikasi Bakteri Aeromonas hydrophila dengan Uji Mikrobiologi pada Ikan Lele Dumbo (Clarias gariepinus) yang dibudidayakan di Kecamatan Baitussalam Kabupaten Aceh Besar. Jurnal Ilmiah Mahasiswa Kelautan Dan Perikanan Unsyiah, 1(2).

Asaduzzaman, M., Wahab, M. A., Verdegem, M. C. J., Huque, S., Salam, M. A., & Azim, M. E. (2008). C/N ratio control and substrate addition for periphyton development jointly enhance freshwater prawn Macrobrachium rosenbergii production in ponds. Aquaculture, 280(1–4). https://doi.org/10.1016/j.aquaculture.2008.04.019

Carvalho, M. J., Martínez-Murcia, A., Esteves, A. C., Correia, A., & Saavedra, M. J. (2012). Phylogenetic diversity, antibiotic resistance and virulence traits of Aeromonas spp. from untreated waters for human consumption. International Journal of Food Microbiology, 159(3). https://doi.org/10.1016/j.ijfoodmicro.2012.09.008

de Melo, L. M. R., Almeida, D., Hofer, E., dos Reis, C. M. F., Theophilo, G. N. D., Santos, A. F. das M., & Vieira, R. H. S. D. F. (2011). Antibiotic resistance of Vibrio parahaemolyticus isolated from pond-reared Litopenaeus vannamei marketed in Natal, Brazil. Brazilian Journal of Microbiology, 42(4), 1463–1469. https://doi.org/10.1590/S1517-83822011000400032

De Schryver, P., Crab, R., Defoirdt, T., Boon, N., & Verstraete, W. (2008). The basics of bio-flocs technology: The added value for aquaculture. Aquaculture, 277(3–4), 125–137. https://doi.org/10.1016/j.aquaculture.2008.02.019

Etviliani, M., Dhengi, S., & Rume, M. I. (2021). Pengaruh Pemberian Pakan Dengan Tambahan Probiotik Terhadap Laju Pertumbuhan Dan Kelangsungan Hidup Ikan Lele Dumbo (Clarias gariepinus). Jurnal Ilmu Kelautan Dan Perikanan, 3(1). Retrieved from http://aquanipa.nusanipa.ac.id/index.php/projemen/article/download/29/41

Figueira, V., Vaz-Moreira, I., Silva, M., & Manaia, C. M. (2011). Diversity and antibiotic resistance of Aeromonas spp. in drinking and waste water treatment plants. Water Research, 45(17). https://doi.org/10.1016/j.watres.2011.08.021

H Dar, G., & Kamili, A. N. (2016). Characterization of Aeromonas sobria Isolated from Fish Rohu (Labeo rohita) Collected from Polluted Pond. Journal of Bacteriology & Parasitology, 7(3). https://doi.org/10.4172/2155-9597.1000273

Hardi, E. H. (2018). Bakteri patogen pada ikan air tawar Aeromonas hydrophila dan Pseudomonas fluorescens. Mulawarman University Press.

Hariati, M., Sitio, F., Jubaedah, D., & Syaifudin, M. (2017). Kelangsungan hidup dan pertumbuhan benih ikan lele (clarias sp.) Pada salinitas media yang berbeda survival and Growth of Juvenile Catfish (Clarias sp.) at Different Media Salinity. Jurnal Akuakultur Rawa Indonesia, 5(1). https://doi.org/10.36706/jari.v5i1.5810

Hazrat Ali, M., Sultana Chowdhury, F., Ashrafuzzaman, M., Al Nayem Chowdhury, M., Rezwan Ul Haque, M., Zinnah, K., & Mahbubur Rahman, M. (2014). Identification, pathogenicity, antibiotic and herbal sensitivity of Edwardsiella tarda causing fish disease in Bangladesh. Current Research in Microbiology and Biotechnology, 2(1), 292-297. Retrieved from https://rb.gy/blrirs

Hermawan, T. E. S., Sudaryono, A., & Prayitno, S. B. (2014). Pengaruh Padat Tebar Berbeda Terhadap Pertumbuhan dan Kelulushidupan Benih Lele (Clarias gariepinus) dalam Media Bioflok. Journal of Aquaculture Management and Technology, 2(3). Retrieved from https://ejournal3.undip.ac.id/index.php/jamt/article/view/5605/5405

Himedialab. (2018). Mueller Hinton Agar. HiMedia Laboratories, 2175(Mic).

Ho, D. T., Lee, Y., Kim, N., Roh, H., Kim, A., & Kim, D.-H. (2023). Complete Genomic Sequences of Two Aeromonas hydrophila Isolates Derived from Diseased Fish in South Korea. Microbiology Resource Announcements, 12(1). https://doi.org/10.1128/mra.00786-22

Indrawati, A., Wulandari, T., Pasaribu, F. H., & Rifai, A. B. (2020). Characterization of aeromonas hydrophila bacteria on dumbo catfish (Clarias gariepinus) from bungo jambi province, indonesia. Ecology, Environment and Conservation, 26. Retrieved from http://www.envirobiotechjournals.com/EEC/26NovSupplIssue2020/EEC-33.pdf

Janda, J. M., & Abbott, S. L. (2010). The genus Aeromonas: Taxonomy, pathogenicity, and infection. Clinical Microbiology Reviews, 23(1), 35–73. https://doi.org/10.1128/CMR.00039-09

Juanda, J., & Jayadi, E. M. (2018). Pengaruh Ekstrak Daun Mimba (Azadirachta indica A. Juss) Terhadap Mortalitas Hama Lalat Buah Cabai (Bactrocera dorsalis L.). Biota, 8(1). https://doi.org/10.20414/jb.v8i1.62

Kementrian kesehatan republik indonesia. (2020). Tetap Produktif, Cegah Dan Atasi Diabetes Mellitus. Pusat data dan informasi kementrian kesehatan RI.

Ko, W. C., Chiang, S. R., Lee, H. C., Tang, H. J., Wang, Y. Y., & Chuang, Y. C. (2003). In vitro and in vivo activities of fluoroquinolones against Aeromonas hydrophila. Antimicrobial Agents and Chemotherapy, 47(7), 2217–2222. https://doi.org/10.1128/AAC.47.7.2217-2222.2003

Lukistyowati, I., & Kurniasih. (2012). Pelacakan Gen Aerolysin dari Aeromonas hydrophila pada Ikan Mas yang Diberi Pakan Ekstrak Bawang Putih. Jurnal Veteriner, 13(1). Retrieved from https://ojs.unud.ac.id/index.php/jvet/article/view/2137/1323

Miyahara, E., Nishie, M., Takumi, S., Miyanohara, H., Nishi, J., Yoshiie, K., Oda, H., Takeuchi, M., Komatsu, M., Aoyama, K., Horiuchi, M., & Takeuchi, T. (2011). Environmental mutagens may be implicated in the emergence of drug-resistant microorganisms. In FEMS Microbiology Letters (Vol. 317, Issue 2). https://doi.org/10.1111/j.1574-6968.2011.02215.x

O’Neill, J. (2016). Antimicrobial Resistance : Tackling a crisis for the health and wealth of nations. Review on Antimicrobial Resistance. Retrieved from https://wellcomecollection.org/works/rdpck35v

Orozova, P., Chikova, V., Kolarova, V., Nenova, R., Konovska, M., & Najdenski, H. (2008). Antibiotic Resistance of Potentially Pathogenic Aeromonas Strains. Trakia Journal of Sciences, 6(C). Retrieved from http://tru.uni-sz.bg/tsj/Vol6N01_2008_supplement/Orozova.pdf

Patil, H. J., Benet-Perelberg, A., Naor, A., Smirnov, M., Ofek, T., Nasser, A., Minz, D., & Cytryn, E. (2016). Evidence of increased antibiotic resistance in phylogenetically-diverse aeromonas isolates from semi-intensive fish ponds treated with antibiotics. Frontiers in Microbiology, 7(NOV). https://doi.org/10.3389/fmicb.2016.01875

Piotrowska, M., & Popowska, M. (2014). The prevalence of antibiotic resistance genes among Aeromonas species in aquatic environments. In Annals of Microbiology (Vol. 64, Issue 3). https://doi.org/10.1007/s13213-014-0911-2

Pratama, I., Talaha, R., Rijal, M. A., & Susylowati, D. (2022). Respon Pertumbuhan dan Daya Tahan Tubuh Benih Ikan Mas Rajadanu (Cyprinus carpio L) yang Diberi Probiotik terhadap Infeksi Aeromonas hydrophila. Sainteks, 19(1). https://doi.org/10.30595/sainteks.v19i1.13288

Qabajah, M., Awwad, E., & Ashhab, Y. (2014). Molecular characterisation of Escherichia coli from dead broiler chickens with signs of colibacillosis and ready-to-market chicken meat in the West Bank. British Poultry Science, 55(4). https://doi.org/10.1080/00071668.2014.935998

Saavedra, M. J., Guedes-Novais, S., Alves, A., Rema, P., Tacão, M., Correia, A., & Martínez-Murcia, A. (2004). Resistance to β-lactam antibiotics in Aeromonas hydrophila isolated from rainbow trout (Oncorhynchus mykiss). International Microbiology, 7(3). Retrieved from https://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1139-67092004000300007

Sari, P. D. W., Mahasri, G., & Koesnoto, K. (2020). Patogenesis Gyrodactylus : penentuan derajat infestasi, pengamatan gejala klinis dan patologi insang ikan mas (Cyprinus carpio). Journal of Aquaculture and Fish Health, 9(1). https://doi.org/10.20473/jafh.v9i1.16215

Sarjito, Prayitno, S. B., & Haditomo, A. H. C. (2013). Buku Pengantar Parasit dan Penyakit Ikan. UPT UNDIP Press Semarang

Sarjito, S.-, Prayitno, S. B., Rochani, N. Q. S., Haditomo, A. H. C., Amalia, R., & Desrina, D. (2020). Potensi epibiotik campuran ekstrak daun binahong (anredera cordifolia) dan temulawak (curcuma zanthorrhiza) pada pakan untuk mengatasi infeksi aeromonas hydrophila pada ikan lele (Clarias gariepinus). Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology, 16(1). https://doi.org/10.14710/ijfst.16.1.51-58

Stratev, D., Daskalov, H., & Vashin, I. (2015). Characterisation and determination of antimicrobial resistance of β-haemolytic Aeromonas spp. isolated from common carp (Cyprinus carpio L.). Revue de Medecine Veterinaire, 166(1–2). Retrieved from https://rb.gy/rpqlc4

Wayne, P. (2006). Clinical and Laboratory Standarts Institute: methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved Standart M7-A7, CLSI, USA.

Wulandari, T., Indrawati, A., & Pasaribu, F. H. (2019). Isolasi dan Identifikasi Aeromonas hydrophila pada Ikan Lele (Clarias gariepinus) Pertambakan Muara Jambi, Provinsi Jambi. Jurnal Medik Veteriner, 2(2), 89. https://doi.org/10.20473/jmv.vol2.iss2.2019.89-95

Yano, Y., Hamano, K., Tsutsui, I., Aue-umneoy, D., Ban, M., & Satomi, M. (2015). Occurrence, molecular characterization, and antimicrobial susceptibility of Aeromonas spp. in marine species of shrimps cultured at inland low salinity ponds. Food Microbiology, 47. https://doi.org/10.1016/j.fm.2014.11.003

Yogananth, N., Bhakyaraj, R., Syed Ali, M., & Muthezhila, R. (2019). Effect of Yeast Elicitor on the Enhancement of Kaempferol from in vivo and in vitro Callus Cultures of Dregea volubilis Benth. Asian Journal of Biological Sciences, 12(2). https://doi.org/10.3923/ajbs.2019.278.283

Yu, J., Koo, B. H., Kim, D. H., Kim, D. W., & Park, S. W. (2015). Aeromonas sobria infection in farmed mud loach (Misgurnus mizolepis) in Korea, a bacteriological survey. Iranian Journal of Veterinary Research, 16(2). Retrieved from https://pubmed.ncbi.nlm.nih.gov/27175175/

Yuhana, M., Normalina, I., & Sukenda. (2008). Pemanfaatan ekstrak bawang putih Allium sativum untuk pencegahan dan pengobatan pada ikan patin Pangasionodon hypophthalmus yang diinfeksi Aeromonas hydrophila. Jurnal Akuakultur Indonesia, 7(1). Retrieved from https://rb.gy/rmx1l0

Zdanowicz, M., Mudryk, Z. J., & Perliński, P. (2020). Abundance and antibiotic resistance of Aeromonas isolated from the water of three carp ponds. Veterinary Research Communications, 44(1). https://doi.org/10.1007/s11259-020-09768-x

Author Biographies

titis wulandari, Universitas Muhammadiyah Muara Bungo

Tri Wiyoko, Universitas Muhammdiyah Muara Bungo

Tri Wera Agrita, Universitas Muhammdiyah Muara Bungo

Nabila Swarna Puspa Hermana, Institut Pertanian Bogor

Agustin Indrawati, Institut Pertanian Bogor

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Copyright (c) 2024 titis wulandari, Tri Wiyoko, Tri Wera Agrita, Nabila Swarna Puspa Hermana, Agustin Indrawati

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