Simultaneous Detection of Glucose and Acetoacetate in Artificial Urine Samples Using 3D-Connector Microfluidic Paper Based Analytical Devices
DOI:
10.29303/jppipa.v10i12.7825Published:
2024-12-18Downloads
Abstract
Diabetic patients sometimes experience both hyperglycemia and hyperketonemia simultaneously. This condition requires proper management of glucose and ketone body biomarkers. The fluctuating intensity of these biomarkers necessitates regular monitoring. This study aims to develop concept non-enzymatic measurement method by integrating µPADs (microfluidic paper-based analytical devices) for detecting glucose and acetoacetate in artificial urine samples. The method uses silver nanoparticle formation for glucose detection and Schiff base reaction as well as the Rothera test for acetoacetate detection. Optimal conditions found include the glucose detection zone with volume ratio of AgNO3 500 mM : starch 3% (w/v) (1 : 1), and the acetoacetate detection zone with glycine 900 mM in phosphate buffer pH 9.4. The artificial urine sample combination consists of glucose, acetoacetate, and acetone with volume ratio of (1 : 1 : 1). The 3D-Connector for glucose uses NaOH 10 M : starch 3% (w/v) with volume ratio of (1 : 3), while for acetoacetate, sodium nitroprusside 15% (w/w) in DMF 5% (v/v). Validity for glucose measurement shows linearity (R² = 0.9664), precision (RSD = 4.56%), accuracy (88.75 – 99.62%), LOD (1.61 mM), and LOQ (5.37 mM). Conversely, acetoacetate measurement shows linearity (R² = 0.9636), precision (RSD = 1.24%), accuracy (99.44 – 99.73%), LOD (1.41 mM), and LOQ (4.69 mM).
Keywords:
Biomarkers Diabetes Non-enzymatic Paper-based devicesReferences
Al-Jaf, S. H., & Omer, K. M. (2022). Enhancing of Detection Resolution Via Designing of a Multi-Functional 3D Connector between Sampling and Detection Zones in Distance-Based Microfluidic Paper-Based Analytical Device: Multi-Channel Design for Multiplex Analysis. Microchimica Acta, 189(482), 1–10. https://doi.org/10.1007/s00604-022-05585-y
Al-Jaf, S. H., & Omer, K. M. (2023). Accuracy Improvement Via Novel Ratiometry Design in Distance-Based Microfluidic Paper Based Analytical Device: Instrument-Free Point of Care Testing. RSC Advances, 13(23), 15704–15713. https://doi.org/10.1039/d3ra01601c
Berber, N., & Arslan, M. (2020). Preparation and Characterization of Some Schiff Base Compounds. Adiyaman University Journal of Science, 10(1), 179–188. https://doi.org/10.37094/adyujsci.633080
Chen, Z., Wright, C., Dincel, O., Chi, T. Y., & Kameoka, J. (2020). A Low-Cost Paper Glucose Sensor with Molecularly Imprinted Polyaniline Electrode. Sensors (Switzerland), 20(4), 1–11. https://doi.org/10.3390/s20041098
Crawford, S. G., Coker, R. H., & Rea, L. D. (2024). Preliminary Comparisons between a Point-of-Care Ketometer and Reference Method Using Steller Sea Lion Pup Whole Blood and Plasma. Conservation Physiology, 12(1), 1–10. https://doi.org/10.1093/conphys/coad104
El-Shishtawy, R. M., Angari, Y. M. A., Alotaibi, M. M., & Almulaiky, Y. Q. (2023). Novel and Facile Colorimetric Detection of Reducing Sugars in Foods Via In Situ Formed Gelatin-Capped Silver Nanoparticles. Polymers, 15(5), 1–13. https://doi.org/10.3390/polym15051086
Fahmi, A. L., ’Aisy, K. R., Wulandari, I. O., Sulistyarti, H., & Sabarudin, A. (2024). Non-Enzymatic Determination of Glucose in Artificial Urine Using 3D-µPADs Through Silver Nanoparticles Formation. Indonesian Journal of Chemistry, 24(5), 1481. https://doi.org/10.22146/ijc.95588
Fall, B., Sall, D. D., Hémadi, M., Diaw, A. K. D., Fall, M., Randriamahazaka, H., & Thomas, S. (2023). Highly Efficient Non-Enzymatic Electrochemical Glucose Sensor Based on Carbon Nanotubes Functionalized by Molybdenum Disulfide and Decorated with Nickel Nanoparticles (GCE/CNT/MoS2/NiNPs). Sensors and Actuators Reports, 5, 1–11. https://doi.org/10.1016/j.snr.2022.100136
Fu, L.-M., Hsu, J.-H., Shih, M.-K., Hsieh, C.-W., Ju, W.-J., Chen, Y.-W., Lee, B. H., & Hou, C.-Y. (2021). Process Optimization of Silver Nanoparticle Synthesis and Its Application in Mercury Detection. Micromachines, 12(9), 1–16. https://doi.org/10.3390/mi12091123
Galicia-Garcia, U., Benito-Vicente, A., Jebari, S., Larrea-Sebal, A., Siddiqi, H., Uribe, K. B., Ostolaza, H., & Martín, C. (2020). Pathophysiology of Type 2 Diabetes Mellitus. International Journal of Molecular Sciences, 21(17), 1–34. https://doi.org/10.3390/ijms21176275
Gonzales, W. V., Mobashsher, A. T., & Abbosh, A. (2019). The Progress of Glucose Monitoring—A Review of Invasive to Minimally and Non-Invasive Techniques, Devices and Sensors. Sensors (Switzerland), 19, 1–45. https://doi.org/10.3390/s19040800
Hassan, E. M., Mushtaq, H., Mahmoud, E. E., Chhibber, S., Saleem, S., Issa, A., Nitesh, J., Jama, A. B., Khedr, A., Boike, S., Mir, M., Attallah, N., Surani, S., & Khan, S. A. (2022). Overlap of Diabetic Ketoacidosis and Hyperosmolar Hyperglycemic State. World Journal of Clinical Cases, 10(32), 11702–11711. https://doi.org/10.12998/wjcc.v10.i32.11702
Hemmati, S., Retzlaff-Roberts, E., Scott, C., & Harris, M. T. (2019). Artificial Sweeteners and Sugar Ingredients as Reducing Agent for Green Synthesis of Silver Nanoparticles. Journal of Nanomaterials, 2019, 1–16. https://doi.org/10.1155/2019/9641860
Hernández-Ramírez, D., Mendoza-Huizar, L. H., Galán-Vidal, C. A., Aguilar-Lira, G. Y., & Álvarez-Romero, G. A. (2022). Development of a Non-Enzymatic Glucose Sensor Based on Fe2O3 Nanoparticles-Carbon Paste Electrodes. Journal of The Electrochemical Society, 169(6), 1–7. https://doi.org/10.1149/1945-7111/ac735c
Hillock, M. F., Jarmon, C., Metropulos, A. E., King, R., Tchernodrinski, S., & Principe, D. R. (2024). Diabetic Ketoacidosis Masked by Both Euglycemia and a Primary Metabolic Alkalosis. Oxford Medical Case Reports, 2024(7), 288–290. https://doi.org/10.1093/omcr/omae071
Hiraoka, R., Kuwahara, K., Wen, Y.-C., Yen, T.-H., Hiruta, Y., Cheng, C.-M., & Citterio, D. (2020). Paper-Based Device for Naked Eye Urinary Albumin/Creatinine Ratio Evaluation. ACS Sensors, 5(4), 1110–1118. https://doi.org/10.1021/acssensors.0c00050
Ibrahim, F. M., & Abdalhadi, S. M. (2021). Performance of Schiff Bases Metal Complexes and Their Ligand in Biological Activity: A Review. Al-Nahrain Journal of Science, 24(1), 1–10. https://doi.org/10.22401/ANJS.24.1.01
Iqbal, M., Zafar, H., Mahmood, A., Niazi, M. B. K., & Aslam, M. W. (2020). Starch-Capped Silver Nanoparticles Impregnated Into Propylamine-Substituted PVA Films with Improved Antibacterial and Mechanical Properties for Wound-Bandage Applications. Polymers, 12(9), 1–17. https://doi.org/10.3390/POLYM12092112
Kausar, F., Sarwar, M., Aslam, A., Jamil, A., Sultana, N., & Shaffqat, G. (2023). Role of Capillary Blood Ketone Assay in Management of Diabetic Ketoacidosis in Paediatric Intensive Care Unit. Pakistan Armed Forces Medical Journal, 73(5), 1448–1451. https://doi.org/10.51253/pafmj.v73i5.9116
Loiseau, A., Asila, V., Boitel-Aullen, G., Lam, M., Salmain, M., & Boujday, S. (2019). Silver-Based Plasmonic Nanoparticles for and Their Use in Biosensing. Biosensors, 9(2), 1–39. https://doi.org/10.3390/bios9020078
Mukhopadhyay, M., Subramanian, S. G., Durga, K. V., Sarkar, D., & DasGupta, S. (2022). Laser Printing Based Colorimetric Paper Sensors for Glucose and Ketone Detection: Design, Fabrication, and Theoretical Analysis. Sensors and Actuators B: Chemical, 371, 1–10. https://doi.org/10.1016/j.snb.2022.132599
Nasser, S., Vialichka, V., Biesiekierska, M., Balcerczyk, A., & Pirola, L. (2020). Effects of Ketogenic Diet and Ketone Bodies on the Cardiovascular System: Concentration Matters. World Journal of Diabetes, 11(12), 584–595. https://doi.org/10.4239/wjd.v11.i12.584
Nguyen, H. T., Nguyen, T. D., Nguyen, D. P., Thai, N. T. T., & Nguyen, T. H. (2022). Synthesis Efficiency of Silver Nanoparticles by Light-Emitting Diode and Microwave Irradiation Using Starch as a Reducing Agent. Nanotechnology for Environmental Engineering, 7(1), 297–306. https://doi.org/10.1007/s41204-022-00231-7
Nguyen, N. P. U., Dang, N. T., Doan, L., & Nguyen, T. T. H. (2023). Synthesis of Silver Nanoparticles: From Conventional to ‘Modern’ Methods—A Review. Processes, 11(9), 1–17. https://doi.org/10.3390/pr11092617
Poian, A. T. D., & Castanho, M. A. R. B. (2021). Integrative Human Biochemistry. In Integrative Human Biochemistry. Cham: Springer International Publishing.
Ponsanti, K., Tangnorawich, B., Ngernyuang, N., & Pechyen, C. (2020). A Flower Shape-Green Synthesis and Characterization of Silver Nanoparticles (AgNPs) with Different Starch as a Reducing Agent. Journal of Materials Research and Technology, 9(5), 11003–11012. https://doi.org/10.1016/j.jmrt.2020.07.077
Ramteke, P., Deb, A., Shepal, V., & Bhat, M. K. (2019). Hyperglycemia Associated Metabolic and Molecular Alterations in Cancer Risk, Progression, Treatment, and Mortality. Cancers, 11(9), 1–23. https://doi.org/10.3390/cancers11091402
Rather, R. A., Sarwara, R. K., Das, N., & Pal, B. (2019). Impact of Reducing and Capping Agents on Carbohydrates for the Growth of Ag and Cu Nanostructures and Their Antibacterial Activities. Particuology, 43, 219–226. https://doi.org/10.1016/j.partic.2018.01.004
Sanchez-Castañeda, M. P., Cañon-Ibarra, A. F., Cruz-Posada, A. F., Sanchez, L. T., Pinzon, M. I., & Villa, C. C. (2020). Synthesis and Characterization of Starch Stabilized Ag Nanoparticles Effect of the Crystalline Structure of Starch. Journal of Physics: Conference Series, 1541(1), 1–6. https://doi.org/10.1088/1742-6596/1541/1/012003
Sayakulu, N. F., & Soloi, S. (2022). The Effect of Sodium Hydroxide (NaOH) Concentration on Oil Palm Empty Fruit Bunch (OPEFB) Cellulose Yield. Journal of Physics: Conference Series, 2314(1), 012017. https://doi.org/10.1088/1742-6596/2314/1/012017
Susana, E., Ramli, K., Purnamasari, P. D., & Apriantoro, N. H. (2023). Non-Invasive Classification of Blood Glucose Level Based on Photoplethysmography Using Time–Frequency Analysis. Information (Switzerland), 14(3), 1–18. https://doi.org/10.3390/info14030145
Taha, M. F., Ashraf, H., & Caesarendra, W. (2020). A Brief Description of Cyclic Voltammetry Transducer-Based Non-Enzymatic Glucose Biosensor Using Synthesized Graphene Electrodes. Applied System Innovation, 3(3), 1–33. https://doi.org/10.3390/asi3030032
Tarmizi, Z. I., Burhanuddin, M. F. B., Shahrul, M., Mokhtar, M. A. M., Zhe, J. C., Taib, S. H. M., & Sukri, S. N. A. (2022). Preparation and Characterization of Acetylated Starch Mediated Silver Nanoparticles: The Effect of Solution Ratio and Time-Varying Exposure. Journal of Physics: Conference Series, 2259(1), 012005. https://doi.org/10.1088/1742-6596/2259/1/012005
Wu, B., Xue, Q., Fan, Y., Cui, D., Sun, Y., Xu, B. B., Wei, H., Li, H., Wasnik, P., Sridhar, D., Algadi, H., Guo, Z., & Annamareddy, S. H. K. (2023). Multifunctional Polymer Nanocomposites for Non-Enzymatic Glucose Detection: A Brief Review. ES Food & Agroforestry, 12, 1–11. https://doi.org/10.30919/esfaf859
Yu, P., Deng, M., Yang, Y., Nie, B., & Zhao, S. (2020). 3D Microfluidic Devices in a Single Piece of Paper for the Simultaneous Determination of Nitrite and Thiocyanate. Sensors (Switzerland), 20(15), 1–15. https://doi.org/10.3390/s20154118
Zhang, Z., Yang, G., He, M., Qi, L., Li, X., & Chen, J. (2022). Synthesis of Silver Nanoparticles and Detection of Glucose via Chemical Reduction with Nanocellulose as Carrier and Stabilizer. International Journal of Molecular Sciences, 23(23), 1–13. https://doi.org/10.3390/ijms232315345
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Copyright (c) 2024 Ahmad Luthfi Fahmi, Kamila Rohadatul ‘Aisy, Ika Oktavia Wulandari, Hermin Sulistyarti, Akhmad Sabarudin
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