Utilization Ecdysterone Hormone of Krokot (Portulaca oleracea) Extract on Molting Activity in Mud Crabs (Scylla serrate)

Authors

Gusti Abdillah Ihsan , Maheno Sri Widodo , Abd. Raheem Faqih , Akhsan Fikrillah Paricahya

DOI:

10.29303/jppipa.v10i10.8074

Published:

2024-10-25

Issue:

Vol. 10 No. 10 (2024): October : In Progress

Keywords:

Ecdysterone, Growth, Percentage Molting, Portulaca oleracea, Molting Period, Scylla serrate

Research Articles

Downloads

How to Cite

Ihsan, G. A., Widodo, M. S., Faqih, A. R., & Paricahya, A. F. (2024). Utilization Ecdysterone Hormone of Krokot (Portulaca oleracea) Extract on Molting Activity in Mud Crabs (Scylla serrate). Jurnal Penelitian Pendidikan IPA, 10(10), 7823–7831. https://doi.org/10.29303/jppipa.v10i10.8074

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Abstract

The Scylla serrate mangrove crab is one of the leading fisheries commodities in terms of exports, only behind shrimp, tuna and shellfish. Crabs have a fairly high price in foreign markets and most of the mud crabs obtained from wild catches are soft shell crabs. In terms of price, cultivating soft shell crabs is quite profitable, but most cultivators have several problems such as long maintenance periods and inconsistent molting times so that they do not experience business continuity. Applying herbal extracts via injection is an effective method because all the substances that trigger molting directly enter the body. Physiological engineering is needed to speed up the molting phase and get the same molting time. This research aims to identify the ecdysterone content in purslane leaves and determine the optimum dose of purslane leaf extract which can encourage skin molting in mud crabs. This research used a Randomized Block Design (RBD). The in silicons test results show that the compound that has the smallest binding affinity is ecdysone, namely -6.80 kcal/mol. Based on analysis of variance, the best molting percentage occurred in treatment B at 55.50%. The fastest molting period occurred in treatment C, namely on days 4 to 29 after injection. Treatments B and C had the highest survival rate, namely 100%, while in terms of increasing body weight and carapace width, the effect of all treatments was not significantly different. The water quality results for all groups were still within the optimum value, temperature 26.40 – 33.80ºC; pH 6.83 – 7.20; salinity 20.70 – 26.70 ppt and dissolved oxygen 4 – 4.52 ppm

 

References

Addisie, M. B. (2022). Evaluating Drinking Water Quality Using Water Quality Parameters and Esthetic Attributes. Air, Soil and Water Research, 15, 117862212210750. https://doi.org/10.1177/11786221221075005

Arif, Y., Singh, P., Bajguz, A., & Hayat, S. (2022). Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones. International Journal of Molecular Sciences, 23(15), 8664. https://doi.org/10.3390/ijms23158664

Bhuiyan, Md. S., Shamsuzzaman, M. M., Hossain, M. M., Mitu, S. J., & Mozumder, M. M. H. (2021). Mud crab (Scylla serrata Forsskal 1775) value chain analysis in the Khulna region of Bangladesh. Aquaculture and Fisheries, 6(3), 330–336. https://doi.org/10.1016/j.aaf.2021.01.004

Boscolo-Galazzo, F., Crichton, K. A., Barker, S., & Pearson, P. N. (2018). Temperature dependency of metabolic rates in the upper ocean: A positive feedback to global climate change? Global and Planetary Change, 170, 201–212. https://doi.org/10.1016/j.gloplacha.2018.08.017

Ceyda-Irkin, L. (2022). The Effects of Shellfish Consumption Frequency for Human Health. In Update on Malacology. IntechOpen. https://doi.org/10.5772/intechopen.100405

Chatterjee, A., Walters, R., Shafi, Z., Ahmed, O. S., Sebek, M., Gysi, D., Yu, R., Eliassi-Rad, T., Barabási, A.-L., & Menichetti, G. (2023). Improving the generalizability of protein-ligand binding predictions with AI-Bind. Nature Communications, 14(1), 1989. https://doi.org/10.1038/s41467-023-37572-z

Chen, S., Shi, C., Migaud, H., Song, C., Mu, C., Ye, Y., Wang, C., & Ren, Z. (2022). Light Spectrum Impacts on Growth, Molting, and Oxidative Stress Response of the Mud Crab Scylla paramamosain. Frontiers in Marine Science, 9, 840353. https://doi.org/10.3389/fmars.2022.840353

Eddiwan, Dahril, T., Adriman, Budijono, Efawani, & Harjoyudanto, Y. (2021). Study of Growth and Survival of Mud Crab (Scylla serrata, Forskal) with Different Salinity Levels in culture media. IOP Conference Series: Earth and Environmental Science, 934(1), 012015. https://doi.org/10.1088/1755-1315/934/1/012015

Fazhan, H., Azra, M. N., Halim, S. A., Naimullah, M., Abualreesh, M. H., Shu-Chien, A. C., Wang, Y., Fujaya, Y., Syahnon, M., Ma, H., Waiho, K., & Ikhwanuddin, M. (2022). Species composition, abundance, size distribution, sex ratios, and movement of Scylla mud crabs within the mangrove ecosystem at Setiu Wetland, Terengganu, Malaysia. Frontiers in Marine Science, 9, 899789. https://doi.org/10.3389/fmars.2022.899789

Friedman, R. (2022). Computational studies of protein–drug binding affinity changes upon mutations in the drug target. WIREs Computational Molecular Science, 12(1), e1563. https://doi.org/10.1002/wcms.1563

Fujaya, Y., Rukminasari, N., Alam, N., Rusdi, M., Fazhan, H., & Waiho, K. (2020). Is limb autotomy really efficient compared to traditional rearing in soft-shell crab (Scylla olivacea) production? Aquaculture Reports, 18, 100432. https://doi.org/10.1016/j.aqrep.2020.100432

Fujaya, Y., Trijuno, D. D., Nikhlani, A., Cahyono, I., & Hasnidar, H. (2013). The Use of Mulberry (Morus alba) Extract in the Mass Production of Blue Swimming Crab (Portunus pelagicus L.) Larvae to Overcome the Mortality Rate Due to Molting Syndrome. Aquatic Science and Technology, 2(1), 1. https://doi.org/10.5296/ast.v2i1.4048

Gajula, S. N. R., Asgar Vora, S., G. Dikundwar, A., & Sonti, R. (2023). In Vitro Drug Metabolism Studies Using Human Liver Microsomes. In U. Ahmad (Ed.), Dosage Forms—Innovation and Future Perspectives. IntechOpen. https://doi.org/10.5772/intechopen.108246

Hastuti, Y. P., Nirmala, K., Suryani, I., & Prasetiyo, S. L. (2019). Environmental characteristics of mangrove forest as a reference for development of mud Crab Scylla serrata cultivation: A case study in Mojo Village, Ulujami, Pemalang. IOP Conference Series: Earth and Environmental Science, 278(1), 012035. https://doi.org/10.1088/1755-1315/278/1/012035

Holleman, G. A., Hooge, I. T. C., Kemner, C., & Hessels, R. S. (2020). The ‘Real-World Approach’ and Its Problems: A Critique of the Term Ecological Validity. Frontiers in Psychology, 11, 721. https://doi.org/10.3389/fpsyg.2020.00721

Indarjo, A., Salim, G., Zein, M., Septian, D., & Bija, S. (2020). The population and mortality characteristics of mangrove crab (Scylla serrata) in the mangrove ecosystem of Tarakan City, Indonesia. Biodiversitas Journal of Biological Diversity, 21(8). https://doi.org/10.13057/biodiv/d210855

Karniati, R., Sulistiyono, N., Amelia, R., Slamet, B., Bimantara, Y., & Basyuni, M. (2021). Mangrove ecosystem in North Sumatran (Indonesia) forests serves as a suitable habitat for mud crabs (Scylla serrata and S. olivacea). Biodiversitas Journal of Biological Diversity, 22(3). https://doi.org/10.13057/biodiv/d220353

Knigge, T., LeBlanc, G. A., & Ford, A. T. (2021). A Crab Is Not a Fish: Unique Aspects of the Crustacean Endocrine System and Considerations for Endocrine Toxicology. Frontiers in Endocrinology, 12, 587608. https://doi.org/10.3389/fendo.2021.587608

Lafont, R., Balducci, C., & Dinan, L. (2021). Ecdysteroids. Encyclopedia, 1(4), 1267–1302. https://doi.org/10.3390/encyclopedia1040096

Li, G., Zhu, G., Liu, J., Wang, Z., Long, H., Zhang, R., & Yu, K. (2024). Effects of stable and fluctuating soil water on the agronomic and biological performance of root vegetables. Frontiers in Plant Science, 15, 1325078. https://doi.org/10.3389/fpls.2024.1325078

Liu, J., Shi, C., Ye, Y., Ma, Z., Mu, C., Ren, Z., Wu, Q., & Wang, C. (2022). Effects of Temperature on Growth, Molting, Feed Intake, and Energy Metabolism of Individually Cultured Juvenile Mud Crab Scylla paramamosain in the Recirculating Aquaculture System. Water, 14(19), 2988. https://doi.org/10.3390/w14192988

Lubis, A. S., Efrizal, E., Syaifullah, S., Rusnam, R., Nurmiati, N., & Puari, A. T. (2023). Growth performance and survival rate of spiny lobster Panulirus homarus (Linnaeus, 1758) with formulated feeding enriched by spinach extract. Biodiversitas Journal of Biological Diversity, 24(11). https://doi.org/10.13057/biodiv/d241121

Mariani, L., Trivellato, B., Martini, M., & Marafioti, E. (2022). Achieving Sustainable Development Goals Through Collaborative Innovation: Evidence from Four European Initiatives. Journal of Business Ethics, 180(4), 1075–1095. https://doi.org/10.1007/s10551-022-05193-z

Mégevand, L., Kreienbühl, P., Theuerkauff, D., Lignot, J.-H., & Sucré, E. (2022). Individual metabolism and behaviour as complementary endpoints to better understand mangrove crab community variations linked to wastewater inputs. Ecotoxicology and Environmental Safety, 236, 113487. https://doi.org/10.1016/j.ecoenv.2022.113487

Ortega, P., & Vitorino, H. A. (2022). HSP70 in Gills and Hepatopancreas of Mangrove Crabs Ucides cordatus: Comparison between Contaminated and Pristine Environments. Ecologies, 3(3), 249–256. https://doi.org/10.3390/ecologies3030019

Ortiz, J., Arístegui, J., Goldenberg, S. U., Fernández-Méndez, M., Taucher, J., Archer, S. D., Baumann, M., & Riebesell, U. (2024). Phytoplankton physiology and functional traits under artificial upwelling with varying Si:N. Frontiers in Marine Science, 10, 1319875. https://doi.org/10.3389/fmars.2023.1319875

Rahma, K., & Nurcahyanti, O. (2021). Therapeutic Effect of Red Spinach (Amaranthus tricolor L.) Extract on Pancreatic MDA Levels Rats (Rattus norvegicus) Exposed to MLD-STZ. Journal of Biomedicine and Translational Research, 7(3), 129–133. https://doi.org/10.14710/jbtr.v7i3.12536

Rao, V., & Poonia, A. (2023). Protein characteristics, amino acid profile, health benefits and methods of extraction and isolation of proteins from some pseudocereals—A review. Food Production, Processing and Nutrition, 5(1), 37. https://doi.org/10.1186/s43014-023-00154-z

Rinaldy, H., Iskandar, J., Cahyandito, M. F., & Sunardi, S. (2023). The potential feasibility of mangrove crab (Scylla serrata) silvofishery aquaculture. Biodiversitas Journal of Biological Diversity, 24(6). https://doi.org/10.13057/biodiv/d240630

Sarintang, Muslimin, & Faisal Suddin, A. (2020). Economic analysis of captivity maize hybrid supporting food self-sufficiency in South Sulawesi. IOP Conference Series: Earth and Environmental Science, 484(1), 012130. https://doi.org/10.1088/1755-1315/484/1/012130

Shi, A., Ma, H., Shi, X., Zhou, W., Pan, W., Song, Y., Chen, Q., Yu, X., Niu, C., Yang, Y., Cheng, Y., & Yang, X. (2023). Effects of microbe-derived antioxidants on growth, digestive and aminotransferase activities, and antioxidant capacities in the hepatopancreas of Eriocheir sinensis under ammonia nitrogen stress. Aquaculture and Fisheries, S2468550X22001927. https://doi.org/10.1016/j.aaf.2022.12.002

Silva, J. A. (2023). Wastewater Treatment and Reuse for Sustainable Water Resources Management: A Systematic Literature Review. Sustainability, 15(14), 10940. https://doi.org/10.3390/su151410940

Sousa, R. D., Bragança, L., Da Silva, M. V., & Oliveira, R. S. (2024). Challenges and Solutions for Sustainable Food Systems: The Potential of Home Hydroponics. Sustainability, 16(2), 817. https://doi.org/10.3390/su16020817

Srijaya, T. C., Pradeep, P. J., Hassan, A., Chatterji, A., Shaharom, F., & Jeffs, A. (2014). Oxygen consumption in trilobite larvae of the mangrove horseshoe crab (Carcinoscorpius rotundicauda; Latreille, 1802): Effect of temperature, salinity, pH, and light–dark cycle. International Aquatic Research, 6(1), 60. https://doi.org/10.1007/s40071-014-0060-z

Sukardi, T., Setiadi, B. R., & Yudantoko, A. (2021). The benefit of running maintenance habituation before learning of machining process begins. Jurnal Pendidikan Vokasi, 11(2), 222–231. https://doi.org/10.21831/jpv.v11i3.44018

Susanto, G. N., Murwani, S., & Zulhafid, M. (2015). Effect of Food Types and Habitats on the Value of Female Maturity Index (Fmi) and Gonado Somatic Index (Gsi) In The Egg Production Of Mangrove CRABS, Scylla serrata. KnE Life Sciences, 2(1), 354. https://doi.org/10.18502/kls.v2i1.173

Syeed, M. M. M., Hossain, M. S., Karim, M. R., Uddin, M. F., Hasan, M., & Khan, R. H. (2023). Surface water quality profiling using the water quality index, pollution index and statistical methods: A critical review. Environmental and Sustainability Indicators, 18, 100247. https://doi.org/10.1016/j.indic.2023.100247

Thirukanthan, C. S., Azra, M. N., Seman, N. J. A., Agos, S. M., Arifin, H., Aouissi, H. A., Lananan, F., & Gao, H. (2023). A scientometric review of climate change and research on crabs. Journal of Sea Research, 193, 102386. https://doi.org/10.1016/j.seares.2023.102386

Widigdo, B., Rukisah, R., Laga, A., Hakim, A. A., & Wardiatno, Y. (2017). Carapace length-weight and width-weight relationships of Scylla serrata in Bulungan District, North Kalimantan, Indonesia. Biodiversitas Journal of Biological Diversity, 18(4), 1316–1323. https://doi.org/10.13057/biodiv/d180405

Author Biographies

Gusti Abdillah Ihsan, Brawijaya University

Maheno Sri Widodo, Brawijaya University, Malang

Abd. Raheem Faqih, Brawijaya University, Malang

Akhsan Fikrillah Paricahya, Brawijaya University, Malang

License

Copyright (c) 2024 Gusti Abdillah Ihsan, Maheno Sri Widodo, Abd. Raheem Faqih, Akhsan Fikrillah Paricahya

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with Jurnal Penelitian Pendidikan IPA, agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC-BY License). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Penelitian Pendidikan IPA.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).